Editor's Note:As a unique system of succession in Tibetan Buddhism,the reincarnation of Living Buddhas is governed by standardized religious rituals,historical conventions,and established principles.Since the Karm...Editor's Note:As a unique system of succession in Tibetan Buddhism,the reincarnation of Living Buddhas is governed by standardized religious rituals,historical conventions,and established principles.Since the Karma Kagyu sect pioneered the reincarnation system of Living Buddhas in the 13th century,this tradition has continued for more than 700 years.Since the Gelug sect adopted the reincarnation practice in the 16th century,resulting in major lineages such as the Dalai Lama and Panchen Erdeni reincarnations,the system has now held a history of over 400 years.Since the Yuan Dynasty(1271-1368),successive central governments of China have,without exception,strengthened administration over reincarnation matters.In the 57th year of the reign of Emperor Qianlong(1792)of the Qing Dynasty,the Golden Urn Lot-Drawing System was formally established.This system has remained in place up to the present day.Demonstrating national sovereignty,governmental authority,as well as the sacredness and impartiality of Buddhism,it has effectively prevented corruption and malpractice during the reincarnation process.展开更多
Moutan Cortex terpene glycoside is derived from the dried root bark of Paeonia suffruticosa Andr.in the Paeoniaceae family,which holds significant value as a traditional Chinese medicine.This study investigated that M...Moutan Cortex terpene glycoside is derived from the dried root bark of Paeonia suffruticosa Andr.in the Paeoniaceae family,which holds significant value as a traditional Chinese medicine.This study investigated that Moutan Cortex terpene glycoside(MCTG)improved diabetic kidney disease(DKD)by targeting sirtuin 1(SIRT1)mediated autophagy pathway.Mechanistic insights were gained using DKD model rats and human umbilical vein endothelial cells(HUVECs)to delineate how MCTG operated in the treatment of DKD.Furthermore,network pharmacology was used to identify the primary metabolic pathways affected by MCTG,with key targets being confirmed through polymerase chain reaction(PCR),Western blot,Transmission electron microscope,immunofluorescence staining and monodansylcadaverine(MDC)staining.Finally,small interfering RNA transfection testified SIRT1 in advanced glycation end-products(AGEs)-induced HUVECs injury.MCTG effectively decreased blood glucose rise in DKD rats and reduced levels of cytokines and biochemical indicators.Network pharmacology revealed that metabolism was the main pathway of Moutan Cortex,and the main targets were verified by PCR and protein experiments.Based on these results,we found that Moutan Cortex could improve DKD and SIRT1 was a potential target.Furthermore,knockdown of SIRT1 attenuated AGEs-induced the expression of Beclin 1 and microtubule-associated protein 1 light chain 3 II/I(LC3 II/I)in HUVECs.In summary,this study demonstrated that Moutan Cortex could alleviate DKD via down-regulating SIRT1-mediated autophagy pathway.展开更多
文摘Editor's Note:As a unique system of succession in Tibetan Buddhism,the reincarnation of Living Buddhas is governed by standardized religious rituals,historical conventions,and established principles.Since the Karma Kagyu sect pioneered the reincarnation system of Living Buddhas in the 13th century,this tradition has continued for more than 700 years.Since the Gelug sect adopted the reincarnation practice in the 16th century,resulting in major lineages such as the Dalai Lama and Panchen Erdeni reincarnations,the system has now held a history of over 400 years.Since the Yuan Dynasty(1271-1368),successive central governments of China have,without exception,strengthened administration over reincarnation matters.In the 57th year of the reign of Emperor Qianlong(1792)of the Qing Dynasty,the Golden Urn Lot-Drawing System was formally established.This system has remained in place up to the present day.Demonstrating national sovereignty,governmental authority,as well as the sacredness and impartiality of Buddhism,it has effectively prevented corruption and malpractice during the reincarnation process.
基金supported by grants from the National Natural Science Foundation of China(82474093,81973536)Jiangsu Province“Blue and Green Project”(184080H10240)+2 种基金Graduate Research Innovation Program of Jiangsu(KYCX23_0871)the National Natural Science Foundation of the Youth Science Fund Project(81703775)Health Research Program of Wuxi Municipal Health Commission(Q202107).
文摘Moutan Cortex terpene glycoside is derived from the dried root bark of Paeonia suffruticosa Andr.in the Paeoniaceae family,which holds significant value as a traditional Chinese medicine.This study investigated that Moutan Cortex terpene glycoside(MCTG)improved diabetic kidney disease(DKD)by targeting sirtuin 1(SIRT1)mediated autophagy pathway.Mechanistic insights were gained using DKD model rats and human umbilical vein endothelial cells(HUVECs)to delineate how MCTG operated in the treatment of DKD.Furthermore,network pharmacology was used to identify the primary metabolic pathways affected by MCTG,with key targets being confirmed through polymerase chain reaction(PCR),Western blot,Transmission electron microscope,immunofluorescence staining and monodansylcadaverine(MDC)staining.Finally,small interfering RNA transfection testified SIRT1 in advanced glycation end-products(AGEs)-induced HUVECs injury.MCTG effectively decreased blood glucose rise in DKD rats and reduced levels of cytokines and biochemical indicators.Network pharmacology revealed that metabolism was the main pathway of Moutan Cortex,and the main targets were verified by PCR and protein experiments.Based on these results,we found that Moutan Cortex could improve DKD and SIRT1 was a potential target.Furthermore,knockdown of SIRT1 attenuated AGEs-induced the expression of Beclin 1 and microtubule-associated protein 1 light chain 3 II/I(LC3 II/I)in HUVECs.In summary,this study demonstrated that Moutan Cortex could alleviate DKD via down-regulating SIRT1-mediated autophagy pathway.
