In this work,response surface methodology(RSM)was employed to model and optimize electrodialysis process for mercury(Hg(II))removal from seaweed extracts.Box-Behnken design(BBD)was utilized to evaluate the effects and...In this work,response surface methodology(RSM)was employed to model and optimize electrodialysis process for mercury(Hg(II))removal from seaweed extracts.Box-Behnken design(BBD)was utilized to evaluate the effects and the interaction of influential variables such as operating voltage,influent flow rate,initial concentration of Hg(II)on the removal rate of Hg(II).The developed regression model for removal rate response was validated by analysis of variance,and presented a good agreement of the experimental data with the quadratic equation with high value coefficient of determination value(R2=0.9913,RAdj 2=0.9678).The optimum operating parameters were determined as 7.17V operating voltage,72.54L h−1 influent flow rate and 5.04mgL−1 initial concentration of mercury.Hg(II)removal rate of 76.45%was acquired under the optimum conditions,which showed good agreement with model-predicted(75.81%)result.The results revealed that electrodialysis can be considered as a promising strategy for removal of Hg(II)from seaweed extracts.展开更多
Electrocoagulation(EC)is a technique commonly used in wastewater treatment to remove biological and chemical contaminants,but the process has the potential to be used in clarifying plant extracts for the isolation and...Electrocoagulation(EC)is a technique commonly used in wastewater treatment to remove biological and chemical contaminants,but the process has the potential to be used in clarifying plant extracts for the isolation and identification of secondary metabolites.Seaweed extracts contain copious amounts of chlorophyll and other pigments that obscure the characterization of secondary metabolites such as phenolic acids and flavonoids.In place of conventional methods that utilize solvents,EC can potentially be applied to clarify and fractionate extracts.In this research,an EC duration of 30 min(22 V,0.3-0.5A)with aluminum electrodes resulted in a significant decrease,about 76%,of chlorophyll and 70%of carotenoids from seaweed extract measured at 666 nm and 410 nm.The decrease in extract green and yellow color intensity also mirrored a decrease in total phenolic content(TPC)of the extract from 54±1.55 mg GAE/g DW to 3.2±0.01 mg GAE/g DW after 30 min of EC.However,the phenolic acid profile of the extract after electrocoagulation via HPLC-RP indicated the removal of an interference probably caused by polymeric compounds from the extract,thus leaving the simple phenolic acids in solution for detection.The major phenolic acids detected in seaweed crude extract were p-coumaric,o-coumaric,ferulic and syringic acid.Flavonoids detected included catechin,epicatechin,quercetin-3-glucoside and rutin.The results of this study show the potential of replacing conventional plant extract purification methods with a green method that requires no additional solvent.展开更多
Background:Dietary supplementation with a fucoidan-rich Ascophyllum nodosum extract(ANE),possessing an in vitro anti-Salmonella Typhimurium activity could be a promising on-farm strategy to control Salmonella infectio...Background:Dietary supplementation with a fucoidan-rich Ascophyllum nodosum extract(ANE),possessing an in vitro anti-Salmonella Typhimurium activity could be a promising on-farm strategy to control Salmonella infection in pigs.The objectives of this study were to:1)evaluate the anti-S.Typhimurium activity of ANE(containing 46.6%fucoidan,18.6%laminarin,10.7%mannitol,4.6%alginate)in vitro,and;2)compare the effects of dietary supplementation with ANE and Zinc oxide(ZnO)on growth performance,Salmonella shedding and selected gut parameters in naturally infected pigs.This was established post-weaning(newly weaned pig experiment)and following regrouping of post-weaned pigs and experimental re-infection with S.Typhimurium(challenge experiment).Results:In the in vitro assay,increasing ANE concentrations led to a linear reduction in S.Typhimurium counts(P<0.05).In the newly weaned pig experiment(12 replicates/treatment),high ANE supplementation increased gain to feed ratio,similar to ZnO supplementation,and reduced faecal Salmonella counts on d 21 compared to the low ANE and control groups(P<0.05).The challenge experiment included thirty-six pigs from the previous experiment that remained on their original dietary treatments(control and high ANE groups with the latter being renamed to ANE group)apart from the ZnO group which transitioned onto a control diet on d 21(ZnO-residual group).These dietary treatments had no effect on performance,faecal scores,Salmonella shedding or colonic and caecal Salmonella counts(P>0.05).ANE supplementation decreased the Enterobacteriaceae counts compared to the control.Enterobacteriaceae counts were also reduced in the ZnO-residual group compared to the control(P<0.05).ANE supplementation decreased the expression of interleukin 22 and transforming growth factor beta 1 in the ileum compared to the control(P<0.05).Conclusions:ANE supplementation was associated with some beneficial changes in the composition of the colonic microbiota,Salmonella shedding,and the expression of inflammatory genes associated with persistent Salmonella infection.展开更多
Microalgae play a key role as primary colonizers of soil,enhancing plant growth and improving soil health.Seed priming is a widely used method to improve seedling performance,counteract soilrelated stresses,and boost ...Microalgae play a key role as primary colonizers of soil,enhancing plant growth and improving soil health.Seed priming is a widely used method to improve seedling performance,counteract soilrelated stresses,and boost plant productivity.Here we investigated the impact of priming dwarf pea seeds with live cells or disrupted cell mass of two microalgae,Desmodesmus sp.MAS1 and Heterochlorella sp.MAS3,on plant growth response and rhizosphere health.Plant growth metrics,rhizosphere health parameters,and nutrient status indicators were investigated 21 days after sowing in two different soils(designated as A and B)with varying pH.Results revealed that priming significantly improved the biochemistry of rhizosphere in soil B(pH 8),with over 30%increases in leaf count and fresh weight compared to soil A(pH 6).While flowering rates remained low,priming with strain MAS1 significantly enhanced chlorophyll(20%),indole-3-acetic acid(61%),and dehydrogenase activity(50%).Furthermore,strain MAS1 boosted nutrient availability in the rhizosphere,with a 30%–60%increase in carbon and nitrogen levels,promoting exopolysaccharide release.Our findings thus demonstrate the potential of seed priming with microalgae in modulating rhizosphere health,thereby enhancing plant growth and productivity.展开更多
基金financially supported by the Key Research Project of Shandong Province (No. 2017CXGC 1004)the National Natural Science Foundation of China (No. 21878178)+1 种基金the Shandong Science and Technology Development Plan (No. 2018GGX107001)the Young Tai- shan Scholars Program of Shandong Province
文摘In this work,response surface methodology(RSM)was employed to model and optimize electrodialysis process for mercury(Hg(II))removal from seaweed extracts.Box-Behnken design(BBD)was utilized to evaluate the effects and the interaction of influential variables such as operating voltage,influent flow rate,initial concentration of Hg(II)on the removal rate of Hg(II).The developed regression model for removal rate response was validated by analysis of variance,and presented a good agreement of the experimental data with the quadratic equation with high value coefficient of determination value(R2=0.9913,RAdj 2=0.9678).The optimum operating parameters were determined as 7.17V operating voltage,72.54L h−1 influent flow rate and 5.04mgL−1 initial concentration of mercury.Hg(II)removal rate of 76.45%was acquired under the optimum conditions,which showed good agreement with model-predicted(75.81%)result.The results revealed that electrodialysis can be considered as a promising strategy for removal of Hg(II)from seaweed extracts.
基金Provided by the Natural Sciences and Engineering Research Council(NSERC)through the RPGPIN-2018-06296 grant.
文摘Electrocoagulation(EC)is a technique commonly used in wastewater treatment to remove biological and chemical contaminants,but the process has the potential to be used in clarifying plant extracts for the isolation and identification of secondary metabolites.Seaweed extracts contain copious amounts of chlorophyll and other pigments that obscure the characterization of secondary metabolites such as phenolic acids and flavonoids.In place of conventional methods that utilize solvents,EC can potentially be applied to clarify and fractionate extracts.In this research,an EC duration of 30 min(22 V,0.3-0.5A)with aluminum electrodes resulted in a significant decrease,about 76%,of chlorophyll and 70%of carotenoids from seaweed extract measured at 666 nm and 410 nm.The decrease in extract green and yellow color intensity also mirrored a decrease in total phenolic content(TPC)of the extract from 54±1.55 mg GAE/g DW to 3.2±0.01 mg GAE/g DW after 30 min of EC.However,the phenolic acid profile of the extract after electrocoagulation via HPLC-RP indicated the removal of an interference probably caused by polymeric compounds from the extract,thus leaving the simple phenolic acids in solution for detection.The major phenolic acids detected in seaweed crude extract were p-coumaric,o-coumaric,ferulic and syringic acid.Flavonoids detected included catechin,epicatechin,quercetin-3-glucoside and rutin.The results of this study show the potential of replacing conventional plant extract purification methods with a green method that requires no additional solvent.
基金supported by the Science Foundation Ireland(SFI)[grant number:14/IA/2548].
文摘Background:Dietary supplementation with a fucoidan-rich Ascophyllum nodosum extract(ANE),possessing an in vitro anti-Salmonella Typhimurium activity could be a promising on-farm strategy to control Salmonella infection in pigs.The objectives of this study were to:1)evaluate the anti-S.Typhimurium activity of ANE(containing 46.6%fucoidan,18.6%laminarin,10.7%mannitol,4.6%alginate)in vitro,and;2)compare the effects of dietary supplementation with ANE and Zinc oxide(ZnO)on growth performance,Salmonella shedding and selected gut parameters in naturally infected pigs.This was established post-weaning(newly weaned pig experiment)and following regrouping of post-weaned pigs and experimental re-infection with S.Typhimurium(challenge experiment).Results:In the in vitro assay,increasing ANE concentrations led to a linear reduction in S.Typhimurium counts(P<0.05).In the newly weaned pig experiment(12 replicates/treatment),high ANE supplementation increased gain to feed ratio,similar to ZnO supplementation,and reduced faecal Salmonella counts on d 21 compared to the low ANE and control groups(P<0.05).The challenge experiment included thirty-six pigs from the previous experiment that remained on their original dietary treatments(control and high ANE groups with the latter being renamed to ANE group)apart from the ZnO group which transitioned onto a control diet on d 21(ZnO-residual group).These dietary treatments had no effect on performance,faecal scores,Salmonella shedding or colonic and caecal Salmonella counts(P>0.05).ANE supplementation decreased the Enterobacteriaceae counts compared to the control.Enterobacteriaceae counts were also reduced in the ZnO-residual group compared to the control(P<0.05).ANE supplementation decreased the expression of interleukin 22 and transforming growth factor beta 1 in the ileum compared to the control(P<0.05).Conclusions:ANE supplementation was associated with some beneficial changes in the composition of the colonic microbiota,Salmonella shedding,and the expression of inflammatory genes associated with persistent Salmonella infection.
基金supported by Wine Australia with levies from Australia’s grape growers and winemakers and matching funds from the Australian Government.
文摘Microalgae play a key role as primary colonizers of soil,enhancing plant growth and improving soil health.Seed priming is a widely used method to improve seedling performance,counteract soilrelated stresses,and boost plant productivity.Here we investigated the impact of priming dwarf pea seeds with live cells or disrupted cell mass of two microalgae,Desmodesmus sp.MAS1 and Heterochlorella sp.MAS3,on plant growth response and rhizosphere health.Plant growth metrics,rhizosphere health parameters,and nutrient status indicators were investigated 21 days after sowing in two different soils(designated as A and B)with varying pH.Results revealed that priming significantly improved the biochemistry of rhizosphere in soil B(pH 8),with over 30%increases in leaf count and fresh weight compared to soil A(pH 6).While flowering rates remained low,priming with strain MAS1 significantly enhanced chlorophyll(20%),indole-3-acetic acid(61%),and dehydrogenase activity(50%).Furthermore,strain MAS1 boosted nutrient availability in the rhizosphere,with a 30%–60%increase in carbon and nitrogen levels,promoting exopolysaccharide release.Our findings thus demonstrate the potential of seed priming with microalgae in modulating rhizosphere health,thereby enhancing plant growth and productivity.
