Selenium(Se)is a necessary micronutrient,while Se-deficiency is common in most areas worldwide.Developing dietary Se-supplements can address this issue.Se-enriched Lentinus edodes are good sources of natural Sepolysac...Selenium(Se)is a necessary micronutrient,while Se-deficiency is common in most areas worldwide.Developing dietary Se-supplements can address this issue.Se-enriched Lentinus edodes are good sources of natural Sepolysaccharides that are promising dietary Se-supplements.However,Se-polysaccharides from Se-enriched Lentinus edodes(Se-P-LE)fruit body via enzymatic-extraction(EE)have only been sparsely studied.Thus,the EE of Se-P-LE was optimized via Box-Behnken and uniform test,and the physicochemical and bio-functional properties of Se-P-LE were investigated herein.The results showed that the optimized EE conditions were:extracting temperature of 45℃,pH of 4.5,time of 85 min and a liquid-solid ratio of 20:1 mL/g.The addition amount of cellulase,papain and pectinase were 0.80,0.65 and 0.8 g/100 g samples,respectively.Under this condition,Se-P-LE yield was 18.16±0.10%,and the Se content of Se-P-LE was 29.51±0.23μg/100 g.Characterization of Se-P-LE showed that Se-P-LE were Se-conjugated polysaccharides with pyranoglycan andβ-glycosidic linkages.Se-P-LE were composed of 8 monosaccharides with shear-thinning properties and microcrystalline/amorphous structures.The bioactivities assays showed that Se-P-LE displayed a higher DPPH and hydroxyl radical scavenging rate than ordinary polysaccharides.Besides,Se-P-LE significantly reduced mRNA expression and levels of pro-inflammation factors(NO,IL-6/1βand TNF-α)caused by LPS in RAW264.7 cell line.展开更多
基金funded by National Natural Science Foundation of China(32160565)Scientific Research Plan Projects of Shaanxi Educa-tion Department,China(21JK0469)+3 种基金Key Research and Development Plan of Shaanxi Province,China(2016TZC-N-1-3)Senior Talent Foundation of An Kang University,China(2021AYQDZR04)Crosswise Research Foundation(2017AYHY021)Key Research Plan(2022AYZD01)of An Kang University,China.
文摘Selenium(Se)is a necessary micronutrient,while Se-deficiency is common in most areas worldwide.Developing dietary Se-supplements can address this issue.Se-enriched Lentinus edodes are good sources of natural Sepolysaccharides that are promising dietary Se-supplements.However,Se-polysaccharides from Se-enriched Lentinus edodes(Se-P-LE)fruit body via enzymatic-extraction(EE)have only been sparsely studied.Thus,the EE of Se-P-LE was optimized via Box-Behnken and uniform test,and the physicochemical and bio-functional properties of Se-P-LE were investigated herein.The results showed that the optimized EE conditions were:extracting temperature of 45℃,pH of 4.5,time of 85 min and a liquid-solid ratio of 20:1 mL/g.The addition amount of cellulase,papain and pectinase were 0.80,0.65 and 0.8 g/100 g samples,respectively.Under this condition,Se-P-LE yield was 18.16±0.10%,and the Se content of Se-P-LE was 29.51±0.23μg/100 g.Characterization of Se-P-LE showed that Se-P-LE were Se-conjugated polysaccharides with pyranoglycan andβ-glycosidic linkages.Se-P-LE were composed of 8 monosaccharides with shear-thinning properties and microcrystalline/amorphous structures.The bioactivities assays showed that Se-P-LE displayed a higher DPPH and hydroxyl radical scavenging rate than ordinary polysaccharides.Besides,Se-P-LE significantly reduced mRNA expression and levels of pro-inflammation factors(NO,IL-6/1βand TNF-α)caused by LPS in RAW264.7 cell line.
