With the rapid development of China's agricultural production and the improvement of people's living standards and health awareness,people's demands for food quality and nutrition have become increasingly ...With the rapid development of China's agricultural production and the improvement of people's living standards and health awareness,people's demands for food quality and nutrition have become increasingly strong. Agricultural products that meet various functional needs such as nutrition,health care and safety are increasingly favored by the general public. Se,one of the trace elements necessary for the human body,has begun to be applied and developed in medicine and other life sciences. Developing Se-enriched agricultural relying on Guangxi's unique Se-enriched soil resources is of great significance to enhance people's quality of life,increase the added value of agricultural products,and promote the transformation and upgrading of modern agriculture in the region. Starting with the development status and advantages of industrial development of Se-enriched agriculture in Guangxi,the main problems existing in the current industrial development of Se-enriched agriculture in Guangxi were summarized,and specific countermeasures were put forward in this paper,so as to provide theoretical support for the development of Se-enriched agriculture in Guangxi.展开更多
This study was conducted to investigate the effect of Se-enriched lactobacillus on antibody of Newcastle Disease Virus(NDV)of broilers.One thousand day-old AA broiler chickens were selected from a chicken farm of Jing...This study was conducted to investigate the effect of Se-enriched lactobacillus on antibody of Newcastle Disease Virus(NDV)of broilers.One thousand day-old AA broiler chickens were selected from a chicken farm of Jinghai County,and the chickens were divided randomly into two groups,which respectively had five repeat groups with 100 chickens each,one named basal diet group,and the other named Se-enriched lactobacillus group.Corn soybean meal diet was selected for basal diet group,while 2%se-enriched lactobacillus was added into corn soybean meal diet for the other group.The method of feeding was free diet,drinking,and routine immunization program was adopted.This test period was 42 d.Two chickens were selected randomly in each repeat for slaughtering and collecting serums on the 14th,28th and 42nd d.Assessments on the effect of Se-enriched lactobacillus in antibody titer of NDV of broilers were made by hemagglutination-inhibition test.The results indicated that antibody titer of NDV was increased significantly by Se-enriched lactobacillus.展开更多
Developing a safe and effective dietary selenium(Se)source to mitigate lead(Pb)toxicity is important for public health.In this study,Se-containing peptides(SePEPs)were extracted and purified from Pleurotus eryngii to ...Developing a safe and effective dietary selenium(Se)source to mitigate lead(Pb)toxicity is important for public health.In this study,Se-containing peptides(SePEPs)were extracted and purified from Pleurotus eryngii to investigate their digestion and absorption characteristics as well as their potential to alleviate lead toxicity.A Caco-2 monolayer model was established to explore the transcellular transport pathways of SePEPs after digestion.Additionally,a co-culture model of Caco-2 and NCTC1469 cells,alongside a chronic Pb exposure mouse model,was employed to elucidate the mechanisms through which SePEPs mitigate lead-induced oxidative liver damage,complemented by molecular docking analyses.The findings demonstrated that SePEPs exhibited excellent processing and digestive stability,with the digested SePEPs(D-SePEPs)showing an apparent permeability coefficient(Papp)higher than 1.0×10^(-5) cm/s,indicating favorable bioavailability.Primarily absorbed via endocytosis by intestinal epithelial cells,D-SePEPs transport was significantly inhibited by wortmannin.Postabsorption,SePEPs significantly upregulated the expression of Nrf2,HO-1,GCLC,GCLM,and NQO1 proteins in the liver,enhancing the activity of antioxidant enzymes,particularly GSH-Px,thereby alleviating Pb-induced hepatic oxidative damage.Moreover,SePEPs intervention significantly decreased the contents of TNF-αand IL-6 in the liver of mice,thereby alleviating Pb-induced hepatic inflammation.Molecular docking results further revealed that specific sequences GFSSeMPGLKQDLVLPR and PDGVSeMLVEGK from SePEPs competed with Nrf2 for the Keap1 protein active pocket,thereby activating the Keap1-Nrf2 pathway.These results suggested that SePEPs can be considered a potential Se-enriched functional food to counteract lead toxicity.展开更多
Se-enriched peptides serve various physiological functions and are crucial sources of Se.This study found the Se content of yellowfin tuna dark meat was 18.34 mg/kg(dry weight),making it an ideal natural source of Se-...Se-enriched peptides serve various physiological functions and are crucial sources of Se.This study found the Se content of yellowfin tuna dark meat was 18.34 mg/kg(dry weight),making it an ideal natural source of Se-enriched peptides.We used five different enzymatic treatments to prepare Se-enriched protein hydrolysates from dark meat.Among them,the trypsin-generated protein hydrolysate showed the highestα-glucosidase inhibition activity.It was then fractionated by ultrafiltration followed by RP-HPLC,and the sequences of Se-enriched peptide obtained were identified from the most active fraction by LC-MS/MS.Subsequently,KPLSeCPK was screened by in silico analyses and validated by theα-glucosidase activity inhibition experiment(IC_(50)=0.828 mM).Molecular docking and inhibition kinetics assay further demonstrated the interaction between KPLSeCPK andα-glucosidase,suggesting its potential as a novel hyperglycemic inhibitor.Moreover,KPLSeCPK lessened IR and boosted antioxidant capability of IR-HepG2 cells.Thus,KPLSeCPK provides a valuable reference for efficiently utilizing natural organic Se.展开更多
AIM： To study the protective effects and mechanisms of Se-enriched lactobacillus on liver injury caused by carbon tetrachloride （CCl4） in mice. METHODS： Seventy-two ICR mice were randomly divided into four groups...AIM： To study the protective effects and mechanisms of Se-enriched lactobacillus on liver injury caused by carbon tetrachloride （CCl4） in mice. METHODS： Seventy-two ICR mice were randomly divided into four groups： normal group, CCl4-induced model group, low Se-enriched lactobacillus treatment group （L-Se group）, and high Se-enriched lactobacillus treatment group （H-Se group）. During a 3-wk experimental period, the common complete diet was orally provided daily for normal group and model group, and the mice in L-Se and H-Se groups were given a diet with 2 and 4 mg of organoselenium from Se-enriched lactobacillus per kg feed, respectively. From the 2nd wk of experiment, the model group, L-Se group, and H-Se group received abdominal cavity injection of olive oil solution containing 500 mL/L CCl4 （0.07 mL/100 g body mass） to induce liver injury, and the normal group was given olive oil on every other day for over 2 wk. In the first 2 wk post injection with CCl4, mice in each group were killed. The specimens of blood, liver tissue, and macrophages in abdominal cavity fluid were taken. Then the activities of the following liver tissue injury-associated enzymes including glutathione peroxidase （GSH-Px）, superoxide dismutase （SOD）, alanine aminotransferase （ALT） and aspartate aminotransferase （AST） as well as malondialdehyde （MDA） content were assayed. Changes of phagocytic rate and phagocytic index in macrophages were observed with Wright-Giemsa stain. Plasma TNF-α level was measured by radioimmunoassay. The level of intracellular free Ca^2＋ （[Ca^2＋]i） in hepatocytes was detected under a laser scanning confocal microscope. RESULTS： During the entire experimental period, the AST and ALT activities in liver were greatly enhanced by CCl4 and completely blunted by both low and high doses of Se-enriched lactobacillus. The Se-enriched lactobacillus- protected liver homogenate GSH-Px and SOD activities were higher or significantly higher than those in model group and were close to those in normal group. CCl4 significantly increased MDA content in liver homogenates, while administration of Se-enriched lactobacillus prevented MDA elevation. Phagocytic rate and phagocytic index of macrophages decreased after CCl4 treatment compared to those in normal control, but they were dramatically rescued by Se-enriched lactobacillus, showing a greatly higher phagocytic function compared to model group. CCl4 could significantly elevate plasma TNF-α and hepatocyte [Ca^2＋]i level, which were also obviously prevented by Se-enriched lactobacillus. CONCLUSION： Se-enriched lactobacillus can intervene in CCl4-induced liver injury in mice by enhancing macrophage function activity to keep normal and beneficial effects, elevating antioxidant-enzyme activities and reducing lipid peroxidation reaction, inhibiting excessive release of TNF-α, preventing the dramatic elevation of [Ca^2＋]i in hepatocytes.展开更多
The production of mushrooms enriched with essential elements, e.g. selenium, for human health is an interesting strategy to improve the functional foods supply. The selenium is an essential mineral and makes part of s...The production of mushrooms enriched with essential elements, e.g. selenium, for human health is an interesting strategy to improve the functional foods supply. The selenium is an essential mineral and makes part of structure of enzymes involved in the oxidative metabolism. However, the selenium effect on the activity of respiratory and lignocellulolytic enzymes has not been considered. The understanding of this effect is important to determine the selenium concentration that increases the mushroom productivity and the degradation rate of the substrate. In this study, it was observed reduction of the respiratory activity of Lentinula edodes (Berk.) Pegler, the shiitake mushroom, in function of the increasing of the sodium selenite concentration in the substrate (p < 0.05). Selenium did not inhibit the activity of the hydrolytic enzymes (cellulase and xylanase), but it increased the activity of the oxidative enzyme (laccase). Respiratory activity of L. edodes has a negative correlation with sodium selenite concentration added in substrate. Thus is important to define the ideal dose of selenium to be added to the substrate for increasing lignocellulosic residues degradation and, consequently, guarantee a higher production of Se-enriched mushrooms.展开更多
Selenium(Se)-enriched Brassica napus L.is a valuable organic Se supplement.In this study,the fermentation broth enriched with organic Se(FFS)was prepared using Lactobacillus plantarum to ferment the substrate of Se-en...Selenium(Se)-enriched Brassica napus L.is a valuable organic Se supplement.In this study,the fermentation broth enriched with organic Se(FFS)was prepared using Lactobacillus plantarum to ferment the substrate of Se-enriched Brassica napus L.Significant increases were observed after fermentation in total sugars,reducing sugars,soluble proteins,total phenolic content(TPC),and total flavonoid content(TFC).The organic Se was retained at a concentration of 54.75 mg/g in the freeze-dried sample.Principal component analysis and cluster analysis showed good separation between the FFS and unfermented(FS)groups.Fragrant 2-ethyloxetane had the highest content among all volatiles,while sinapine had the highest content among all phenolic compounds.The fermentation process showed remarkable improvement in the abundance and concentration of volatile compounds and phenolic contents,making FFS exhibit strong antioxidant activity and inhibitory capacity againstα-glucosidase activity.The bioaccessibility of phenolic compounds was significantly greater in FFS compared to FS.ADMET analysis revealed that the majority of phenolic compounds contained in FFS did not exhibit mutagenicity toxicity,hepatotoxicity,skin sensitization,or blood-brain barrier penetration,indicating a favorable level of biosafety.Overall,our study provides a new insight into the further utilization of Se-enriched Brassica napus L.in foods.展开更多
The speciation of Se varies among different plants.Predominant Se species in the protein digestion products of Cardamine violifolia(H-CVP),soybean(H-SBP),and cabbage(H-CBP)are SeCys_(2),SeMet,and Se(VI),respec-tively....The speciation of Se varies among different plants.Predominant Se species in the protein digestion products of Cardamine violifolia(H-CVP),soybean(H-SBP),and cabbage(H-CBP)are SeCys_(2),SeMet,and Se(VI),respec-tively.This study aims to elucidate the neuroprotective effects,bioavailability and transport mechanism of Se in Se-enriched proteins with different Se species.Compared to low-Se protein digestion products,Se-enriched protein digestion products significantly reduced Aβ_(1-42) induced apoptosis in PC-12 cells at the same protein matrix,especially H-CVP(increased by 32.4%relative to the control group).These results indicated that Se enrichment significantly enhances the neuroprotective effects of plant-derived proteins.In addition,H-SBP exerted neuroprotective effects by reducing caspase-9 activity by 42%,whereas H-CVP significantly decreased caspase-3 activity by 29%.The bioavailability of total Se in H-CVP,H-SBP and H-CBP were 29.07%,44.90%and 26.11%,respectively.Se species might also influence the bioavailability of Se,thereby affecting its neuro-protective activity.Furthermore,the transport of the three Se-enriched proteins was energy-dependent and some active transport carriers may be involved in their transmembrane transport.The novel organic cation and plasma membrane monoamine transporter families were participated the absorption of Se while multidrug resistance related protein families regulated the effluxion of Se in H-SBP and H-CVP group.For the H-CBP,organic anion transporters and P-protein families influenced the absorption and effluxion of Se.This study provides a basis for further studies on the roles of different Se species in the neuroprotective effects and bioavailability of Se-enriched proteins.展开更多
Cardamine violifolia is a kind of Selenium(Se)-enriched plant found in China.In this study,Se-enriched protein was extracted and purified form C.violifolia.The changes in total Se,Se species and molecular weight distr...Cardamine violifolia is a kind of Selenium(Se)-enriched plant found in China.In this study,Se-enriched protein was extracted and purified form C.violifolia.The changes in total Se,Se species and molecular weight distribution of protein were explored during in vitro simulated gastrointestinal digestion.The THT fluorescence staining experiment and PC12 cell model were performed to screen for active digestion product.Highperformance liquid chromatography tandem mass spectrometry(HPLC-MS/MS)was used to identify active Seenriched peptides.The results showed that the bioaccessibility of organic Se was higher than that of inorganic Se,and the SP3 fraction in digestive products exhibited notable Aβ1-42 aggregation inhibitory activity and maintained a high cell viability of 73.01%in Aβ1-42-induced PC12 cells.The Se-enriched peptides present in the SP3 fraction,identified by HPLC-MS/MS as KSeCSeCPK,VQRSeMDQ,VESeCLKK,KSSeCSK,VERSeCK,CSeCGAP,STTSeMY and KHSeCAA,may have potential applications in neuroprotection,providing a scientific basis for the prevention and intervention of neurodegenerative diseases.展开更多
Hyperuricemia and selenium(Se)deficiency have become world wide health issues,necessitating the development of new technologies for the prevention of hyperuricemia and daily Se supplementation.This study explored a fe...Hyperuricemia and selenium(Se)deficiency have become world wide health issues,necessitating the development of new technologies for the prevention of hyperuricemia and daily Se supplementation.This study explored a feasible way by providing new lactic acid bacteria(LAB)with both Se-enrichment and purine-lowering functions.The expected LAB were firstly screened out and identified from fermented tea grown in seleniferous areas by a five-step screening method.Among thirty-six candidate strains exhibiting superior Se-enriched ability,seven of them showed excellent uric acid absorption,in vitro xanthine oxidase and uric acid oxidase activities.In particular,Lactobacillus plantarum L123 and Lactococcus lactis L126 degraded inosine and guanosine to hypoxanthine and guanine with degradation rates of 100%,96.88%?0.33%and 93.61%?1.06%,81.15%?1.83%,respectively,and metabolized the substrates of hypoxanthine,xanthine and uric acid into the final product allantoin.Their clearing rates of three free radicals(DPPH,·OH and ABTS+·free radical)were higher than 94%after reaction for 30 min.More than 85%of L123 and 50%of L126 could survive at pH 1.5 for 3 h,while their Se-enriched counterparts showed more enhanced acid tolerance.Both L123 and L126 with or without Se-enrichment tolerated 0.5%bile salt with survival rates above 80%.These Novel LAB provide a promising avenue for the development of new health food and pharmaceutical products that combine Se supplementation with hyperuricemia treatment.展开更多
基金Supported by Science and Technology Plan Project of Guangxi Zhuang Autonomous Region(GK-AA17202037,GK-AB16380164,2015GXNSFAA139101)Science and Technology Project of Xixiangtang,Nanning(201710304)
文摘With the rapid development of China's agricultural production and the improvement of people's living standards and health awareness,people's demands for food quality and nutrition have become increasingly strong. Agricultural products that meet various functional needs such as nutrition,health care and safety are increasingly favored by the general public. Se,one of the trace elements necessary for the human body,has begun to be applied and developed in medicine and other life sciences. Developing Se-enriched agricultural relying on Guangxi's unique Se-enriched soil resources is of great significance to enhance people's quality of life,increase the added value of agricultural products,and promote the transformation and upgrading of modern agriculture in the region. Starting with the development status and advantages of industrial development of Se-enriched agriculture in Guangxi,the main problems existing in the current industrial development of Se-enriched agriculture in Guangxi were summarized,and specific countermeasures were put forward in this paper,so as to provide theoretical support for the development of Se-enriched agriculture in Guangxi.
基金National Natural Science Foundation of China(31101867)Project of Tianjin Agricultural University Affiliated Animal Hospital(ZH004903)。
文摘This study was conducted to investigate the effect of Se-enriched lactobacillus on antibody of Newcastle Disease Virus(NDV)of broilers.One thousand day-old AA broiler chickens were selected from a chicken farm of Jinghai County,and the chickens were divided randomly into two groups,which respectively had five repeat groups with 100 chickens each,one named basal diet group,and the other named Se-enriched lactobacillus group.Corn soybean meal diet was selected for basal diet group,while 2%se-enriched lactobacillus was added into corn soybean meal diet for the other group.The method of feeding was free diet,drinking,and routine immunization program was adopted.This test period was 42 d.Two chickens were selected randomly in each repeat for slaughtering and collecting serums on the 14th,28th and 42nd d.Assessments on the effect of Se-enriched lactobacillus in antibody titer of NDV of broilers were made by hemagglutination-inhibition test.The results indicated that antibody titer of NDV was increased significantly by Se-enriched lactobacillus.
基金supported by the National Natural Science Foundation of China[grant number 32072213]China Scholarship Council.
文摘Developing a safe and effective dietary selenium(Se)source to mitigate lead(Pb)toxicity is important for public health.In this study,Se-containing peptides(SePEPs)were extracted and purified from Pleurotus eryngii to investigate their digestion and absorption characteristics as well as their potential to alleviate lead toxicity.A Caco-2 monolayer model was established to explore the transcellular transport pathways of SePEPs after digestion.Additionally,a co-culture model of Caco-2 and NCTC1469 cells,alongside a chronic Pb exposure mouse model,was employed to elucidate the mechanisms through which SePEPs mitigate lead-induced oxidative liver damage,complemented by molecular docking analyses.The findings demonstrated that SePEPs exhibited excellent processing and digestive stability,with the digested SePEPs(D-SePEPs)showing an apparent permeability coefficient(Papp)higher than 1.0×10^(-5) cm/s,indicating favorable bioavailability.Primarily absorbed via endocytosis by intestinal epithelial cells,D-SePEPs transport was significantly inhibited by wortmannin.Postabsorption,SePEPs significantly upregulated the expression of Nrf2,HO-1,GCLC,GCLM,and NQO1 proteins in the liver,enhancing the activity of antioxidant enzymes,particularly GSH-Px,thereby alleviating Pb-induced hepatic oxidative damage.Moreover,SePEPs intervention significantly decreased the contents of TNF-αand IL-6 in the liver of mice,thereby alleviating Pb-induced hepatic inflammation.Molecular docking results further revealed that specific sequences GFSSeMPGLKQDLVLPR and PDGVSeMLVEGK from SePEPs competed with Nrf2 for the Keap1 protein active pocket,thereby activating the Keap1-Nrf2 pathway.These results suggested that SePEPs can be considered a potential Se-enriched functional food to counteract lead toxicity.
基金funded by the National Natural Science Foundation of China(32360578).
文摘Se-enriched peptides serve various physiological functions and are crucial sources of Se.This study found the Se content of yellowfin tuna dark meat was 18.34 mg/kg(dry weight),making it an ideal natural source of Se-enriched peptides.We used five different enzymatic treatments to prepare Se-enriched protein hydrolysates from dark meat.Among them,the trypsin-generated protein hydrolysate showed the highestα-glucosidase inhibition activity.It was then fractionated by ultrafiltration followed by RP-HPLC,and the sequences of Se-enriched peptide obtained were identified from the most active fraction by LC-MS/MS.Subsequently,KPLSeCPK was screened by in silico analyses and validated by theα-glucosidase activity inhibition experiment(IC_(50)=0.828 mM).Molecular docking and inhibition kinetics assay further demonstrated the interaction between KPLSeCPK andα-glucosidase,suggesting its potential as a novel hyperglycemic inhibitor.Moreover,KPLSeCPK lessened IR and boosted antioxidant capability of IR-HepG2 cells.Thus,KPLSeCPK provides a valuable reference for efficiently utilizing natural organic Se.
基金Supported by the Special Programs of State Science and Technology Ministry of China During the 10~(th) 5-Year Plan Period, No. 2002BA518 A12, and Open Foundation from Key Laboratory of Resource Biotechnology of Jiangsu Province, China, No. KJS00033
文摘AIM： To study the protective effects and mechanisms of Se-enriched lactobacillus on liver injury caused by carbon tetrachloride （CCl4） in mice. METHODS： Seventy-two ICR mice were randomly divided into four groups： normal group, CCl4-induced model group, low Se-enriched lactobacillus treatment group （L-Se group）, and high Se-enriched lactobacillus treatment group （H-Se group）. During a 3-wk experimental period, the common complete diet was orally provided daily for normal group and model group, and the mice in L-Se and H-Se groups were given a diet with 2 and 4 mg of organoselenium from Se-enriched lactobacillus per kg feed, respectively. From the 2nd wk of experiment, the model group, L-Se group, and H-Se group received abdominal cavity injection of olive oil solution containing 500 mL/L CCl4 （0.07 mL/100 g body mass） to induce liver injury, and the normal group was given olive oil on every other day for over 2 wk. In the first 2 wk post injection with CCl4, mice in each group were killed. The specimens of blood, liver tissue, and macrophages in abdominal cavity fluid were taken. Then the activities of the following liver tissue injury-associated enzymes including glutathione peroxidase （GSH-Px）, superoxide dismutase （SOD）, alanine aminotransferase （ALT） and aspartate aminotransferase （AST） as well as malondialdehyde （MDA） content were assayed. Changes of phagocytic rate and phagocytic index in macrophages were observed with Wright-Giemsa stain. Plasma TNF-α level was measured by radioimmunoassay. The level of intracellular free Ca^2＋ （[Ca^2＋]i） in hepatocytes was detected under a laser scanning confocal microscope. RESULTS： During the entire experimental period, the AST and ALT activities in liver were greatly enhanced by CCl4 and completely blunted by both low and high doses of Se-enriched lactobacillus. The Se-enriched lactobacillus- protected liver homogenate GSH-Px and SOD activities were higher or significantly higher than those in model group and were close to those in normal group. CCl4 significantly increased MDA content in liver homogenates, while administration of Se-enriched lactobacillus prevented MDA elevation. Phagocytic rate and phagocytic index of macrophages decreased after CCl4 treatment compared to those in normal control, but they were dramatically rescued by Se-enriched lactobacillus, showing a greatly higher phagocytic function compared to model group. CCl4 could significantly elevate plasma TNF-α and hepatocyte [Ca^2＋]i level, which were also obviously prevented by Se-enriched lactobacillus. CONCLUSION： Se-enriched lactobacillus can intervene in CCl4-induced liver injury in mice by enhancing macrophage function activity to keep normal and beneficial effects, elevating antioxidant-enzyme activities and reducing lipid peroxidation reaction, inhibiting excessive release of TNF-α, preventing the dramatic elevation of [Ca^2＋]i in hepatocytes.
文摘The production of mushrooms enriched with essential elements, e.g. selenium, for human health is an interesting strategy to improve the functional foods supply. The selenium is an essential mineral and makes part of structure of enzymes involved in the oxidative metabolism. However, the selenium effect on the activity of respiratory and lignocellulolytic enzymes has not been considered. The understanding of this effect is important to determine the selenium concentration that increases the mushroom productivity and the degradation rate of the substrate. In this study, it was observed reduction of the respiratory activity of Lentinula edodes (Berk.) Pegler, the shiitake mushroom, in function of the increasing of the sodium selenite concentration in the substrate (p < 0.05). Selenium did not inhibit the activity of the hydrolytic enzymes (cellulase and xylanase), but it increased the activity of the oxidative enzyme (laccase). Respiratory activity of L. edodes has a negative correlation with sodium selenite concentration added in substrate. Thus is important to define the ideal dose of selenium to be added to the substrate for increasing lignocellulosic residues degradation and, consequently, guarantee a higher production of Se-enriched mushrooms.
基金supported by the National Natural Science Foundation of China(U21A20274,31972041)the Agricultural Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2021-OCRI)+1 种基金Earmarked Fund for China Agriculture Research System(CARS-12)Support Enterprise Technology Innovation and Development Projects(2021BLB151)。
文摘Selenium(Se)-enriched Brassica napus L.is a valuable organic Se supplement.In this study,the fermentation broth enriched with organic Se(FFS)was prepared using Lactobacillus plantarum to ferment the substrate of Se-enriched Brassica napus L.Significant increases were observed after fermentation in total sugars,reducing sugars,soluble proteins,total phenolic content(TPC),and total flavonoid content(TFC).The organic Se was retained at a concentration of 54.75 mg/g in the freeze-dried sample.Principal component analysis and cluster analysis showed good separation between the FFS and unfermented(FS)groups.Fragrant 2-ethyloxetane had the highest content among all volatiles,while sinapine had the highest content among all phenolic compounds.The fermentation process showed remarkable improvement in the abundance and concentration of volatile compounds and phenolic contents,making FFS exhibit strong antioxidant activity and inhibitory capacity againstα-glucosidase activity.The bioaccessibility of phenolic compounds was significantly greater in FFS compared to FS.ADMET analysis revealed that the majority of phenolic compounds contained in FFS did not exhibit mutagenicity toxicity,hepatotoxicity,skin sensitization,or blood-brain barrier penetration,indicating a favorable level of biosafety.Overall,our study provides a new insight into the further utilization of Se-enriched Brassica napus L.in foods.
基金supported financially by the National Natural Science Foundation of China(No.32172197,32272314)program of“Collab-orative Innovation Center of Food Safety and Quality Control in Jiangsu Province”,China.
文摘The speciation of Se varies among different plants.Predominant Se species in the protein digestion products of Cardamine violifolia(H-CVP),soybean(H-SBP),and cabbage(H-CBP)are SeCys_(2),SeMet,and Se(VI),respec-tively.This study aims to elucidate the neuroprotective effects,bioavailability and transport mechanism of Se in Se-enriched proteins with different Se species.Compared to low-Se protein digestion products,Se-enriched protein digestion products significantly reduced Aβ_(1-42) induced apoptosis in PC-12 cells at the same protein matrix,especially H-CVP(increased by 32.4%relative to the control group).These results indicated that Se enrichment significantly enhances the neuroprotective effects of plant-derived proteins.In addition,H-SBP exerted neuroprotective effects by reducing caspase-9 activity by 42%,whereas H-CVP significantly decreased caspase-3 activity by 29%.The bioavailability of total Se in H-CVP,H-SBP and H-CBP were 29.07%,44.90%and 26.11%,respectively.Se species might also influence the bioavailability of Se,thereby affecting its neuro-protective activity.Furthermore,the transport of the three Se-enriched proteins was energy-dependent and some active transport carriers may be involved in their transmembrane transport.The novel organic cation and plasma membrane monoamine transporter families were participated the absorption of Se while multidrug resistance related protein families regulated the effluxion of Se in H-SBP and H-CVP group.For the H-CBP,organic anion transporters and P-protein families influenced the absorption and effluxion of Se.This study provides a basis for further studies on the roles of different Se species in the neuroprotective effects and bioavailability of Se-enriched proteins.
基金supported financially by the National Natural Science Foundation of China(No.32172197,32272314)the Innovative Project of the State Key Laboratory of Food Science and Technology(SKLF-ZZB-202307)program of“Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province”,China.
文摘Cardamine violifolia is a kind of Selenium(Se)-enriched plant found in China.In this study,Se-enriched protein was extracted and purified form C.violifolia.The changes in total Se,Se species and molecular weight distribution of protein were explored during in vitro simulated gastrointestinal digestion.The THT fluorescence staining experiment and PC12 cell model were performed to screen for active digestion product.Highperformance liquid chromatography tandem mass spectrometry(HPLC-MS/MS)was used to identify active Seenriched peptides.The results showed that the bioaccessibility of organic Se was higher than that of inorganic Se,and the SP3 fraction in digestive products exhibited notable Aβ1-42 aggregation inhibitory activity and maintained a high cell viability of 73.01%in Aβ1-42-induced PC12 cells.The Se-enriched peptides present in the SP3 fraction,identified by HPLC-MS/MS as KSeCSeCPK,VQRSeMDQ,VESeCLKK,KSSeCSK,VERSeCK,CSeCGAP,STTSeMY and KHSeCAA,may have potential applications in neuroprotection,providing a scientific basis for the prevention and intervention of neurodegenerative diseases.
基金funded by National Natural Science Foundation of China(No.32360564)Bama County Program for Talents in Science and Technology(Nos.BaRenKe20210045,BaRenKe 20220004)Natural Science and Technology Innovation Development Multiplication Plan of Guangxi University(No.2022BZRC010).
文摘Hyperuricemia and selenium(Se)deficiency have become world wide health issues,necessitating the development of new technologies for the prevention of hyperuricemia and daily Se supplementation.This study explored a feasible way by providing new lactic acid bacteria(LAB)with both Se-enrichment and purine-lowering functions.The expected LAB were firstly screened out and identified from fermented tea grown in seleniferous areas by a five-step screening method.Among thirty-six candidate strains exhibiting superior Se-enriched ability,seven of them showed excellent uric acid absorption,in vitro xanthine oxidase and uric acid oxidase activities.In particular,Lactobacillus plantarum L123 and Lactococcus lactis L126 degraded inosine and guanosine to hypoxanthine and guanine with degradation rates of 100%,96.88%?0.33%and 93.61%?1.06%,81.15%?1.83%,respectively,and metabolized the substrates of hypoxanthine,xanthine and uric acid into the final product allantoin.Their clearing rates of three free radicals(DPPH,·OH and ABTS+·free radical)were higher than 94%after reaction for 30 min.More than 85%of L123 and 50%of L126 could survive at pH 1.5 for 3 h,while their Se-enriched counterparts showed more enhanced acid tolerance.Both L123 and L126 with or without Se-enrichment tolerated 0.5%bile salt with survival rates above 80%.These Novel LAB provide a promising avenue for the development of new health food and pharmaceutical products that combine Se supplementation with hyperuricemia treatment.
