Developing flexible bioelectronics is essential to the realization of artificial intelligence devices and biomedical applications, such as wearables, but their potential is limited by sustainable energy supply. An enz...Developing flexible bioelectronics is essential to the realization of artificial intelligence devices and biomedical applications, such as wearables, but their potential is limited by sustainable energy supply. An enzymatic biofuel cell(BFC) is promising for power supply, but its use is limited by the challenges of incorporating multiple enzymes and rigid platforms. This paper shows the first example of screen-printable nanocomposite inks engineered for a single-enzyme-based energy-harvesting device and a self-powered biosensor driven by glucose on bioanode and biocathode. The anode ink is modified with naphthoquinone and multiwalled carbon nanotubes(MWCNTs), whereas the cathode ink is modified with Prussian blue/MWCNT hybrid before immobilizing with glucose oxidase. The flexible bioanode and the biocathode consume glucose. This BFC yields an open circuit voltage of 0.45 V and a maximum power density of 266 μW cm-2. The wearable device coupled with a wireless portable system can convert chemical energy into electric energy and detect glucose in artificial sweat. The self-powered sensor can detect glucose concentrations up to 10 mM. Common interfering substances,including lactate, uric acid, ascorbic acid, and creatinine, have no effect on this self-powered biosensor. Additionally, the device can endure multiple mechanical deformations. New advances in ink development and flexible platforms enable a wide range of applications, including on-body electronics, self-sustainable applications, and smart fabrics.展开更多
CuO nanoparticles were successfully synthesized via a two-jet electrospun method,and then screen-printed on silver-carbon electrodes,forming CuO-modified Ag-C(CuO/Ag-C)disposable strip electrodes.In natural environmen...CuO nanoparticles were successfully synthesized via a two-jet electrospun method,and then screen-printed on silver-carbon electrodes,forming CuO-modified Ag-C(CuO/Ag-C)disposable strip electrodes.In natural environment condition for glucose detection,the obtained CuO/Ag-C electrodes show a high sensitivity of 540 nA·mM^(-1)·cm^(-2),and a low limit of detection(0.68 mM)in a wide linear response range of 0.68 mM and 3 mM(signal/noise=3),respectively.In addition,the CuO/Ag-C electrodes also exhibit excellent anti-interference,air stability and repeatability.As a result,the fabrication of CuO nanoparticles via an electrospun process and the technique of screen-printed electrodes are of great significance for glucose detection.展开更多
Wearable flexible sensor devices have the characteristics of lightweight and miniaturization.Currently,power supply and detection components limit the portability of wearable flexible sensor devices.Meanwhile,conventi...Wearable flexible sensor devices have the characteristics of lightweight and miniaturization.Currently,power supply and detection components limit the portability of wearable flexible sensor devices.Meanwhile,conventional liquid electrolytes are unsuitable for the integration of sensing devices.To address these constraints,wearable biofuel cells and flexible electrochromic displays have been introduced,which can improve integration with other devices,safety,and color-coded display data.Meanwhile,electrode chips prepared through screen printing technology can further improve portability.In this work,a wearable sensor device with screen-printed chips was constructed and used for non-invasive detection of glucose.Agarose gel electrolytes doped with PDA-CNTs were prepared,and the mechanical strength and moisture retention were significantly improved compared with traditional gel electrolytes.Glucose in interstitial fluid was non-invasive extracted to the skin surface using reverse iontophoresis.As a biofuel for wearable biofuel cells,glucose drives self-powered sensor and electrochromic display to produce color change,allowing for visually measurement of glucose levels in body fluids.Accurate detection results can be visualized by reading the RGB value with a cell phone.展开更多
Even in small concentrations,toxic metals like lead,cadmium,and mercury are dangerous to the environment and human health.Environmental monitoring depends on precisely identifying these heavy metals,particularly cadmi...Even in small concentrations,toxic metals like lead,cadmium,and mercury are dangerous to the environment and human health.Environmental monitoring depends on precisely identifying these heavy metals,particularly cadmium ions(Cd(Ⅱ)).In this study,we present a novel screen-printed carbon electrode(SPCE)modified with single crystallineα-Fe_(2)O_(3)nano-hexagons that functions as a sensor for detecting Cd(Ⅱ).The performance of the fabricated sensor was thoroughly assessed and compared with unmodified SPCE using the voltammetric method.The crystalline structure of the synthesizedα-Fe_(2)O_(3)nano-hexagons was confirmed through XRD,and surface analysis revealed an average diameter and thickness of 86 nm and 9 nm,respectively.Theα-Fe_(2)O_(3)modified SPCE yields a 7-fold enhanced response(at pH 5.0 vs.Ag/AgCl)to Cd(Ⅱ)than bare SPCE.The modified electrode effectively detects Cd(Ⅱ)with a linear response range of up to 333.0μmol/L and a detection limit of 0.65 nmol/L under ideal circumstances.This newly fabricated sensor offers significant potential for environmental monitoring applications by providing outstanding practicality,anti-interference ability,and repeatability for detecting Cd(Ⅱ)in water samples.展开更多
A novel electrochemical method for the rapid detection of organophosphorus pesticide residues was realized on a dual-channel screen-printed electrode （DSPE） that was integrated with a portable smartphone-controlled ...A novel electrochemical method for the rapid detection of organophosphorus pesticide residues was realized on a dual-channel screen-printed electrode （DSPE） that was integrated with a portable smartphone-controlled potentiostat. The two carbon working channels of DSPE were first modified by electrodepositing of Prussian blue. The channels were then modified with acetylcholinesterase （ACHE） via Nation. The inhibition ratio of AChE was detected by comparing the electrical current of acetylthiocholine （ATCh） that was catalyzed by the enzyme electrodes with （channel 1） and without （channel 2） organophosphorus pesticide. Inhibition ratios were related with the negative logarithm of the organophosphorus pesticide （trichlorfon, oxamyl, and isocarbophos） concentrations at optimum experimental conditions （pH 6.9 of electrolyte, 0.2V working potential, 2.5μL AChE modification amount, and 15 min inhibition time）. The linear equations were 1%=32.301gC＋ 253.3 （R=0.9750） for isocarbophos, I% = 35.991gC＋ 270.1 （R = 0.9668） for chlorpyrifos, and 1% = 33.701gC＋ 250.5 （R = 0.9606） for trichlorfon. The detection limits were calculated as 10-7 g/mL. Given that the inhibition ratios were only related with pesticide concentration and not with pesticide species, the proposed electrodes and electrometer can rapidly detect universal organophosphorus pesticides and assess pesticide pollution.展开更多
A sensitive and specific immunosensor for the detection of the hormones cortisol and lactate in human or animal biological fluids, such as sweat and saliva, was devised using the label-free electrochemical chronoamper...A sensitive and specific immunosensor for the detection of the hormones cortisol and lactate in human or animal biological fluids, such as sweat and saliva, was devised using the label-free electrochemical chronoamperometric technique. By using these fluids instead of blood,the biosensor becomes noninvasive and is less stressful to the end user, who may be a small child or a farm animal.Electroreduced graphene oxide(e-RGO) was used as a synergistic platform for signal amplification and template for bioconjugation for the sensing mechanism on a screenprinted electrode. The cortisol and lactate antibodies were bioconjugated to the e-RGO using covalent carbodiimide chemistry. Label-free electrochemical chronoamperometric detection was used to analyze the response to the desired biomolecules over the wide detection range. A detection limit of 0.1 ng mL^(-1) for cortisol and 0.1 mM for lactate was established and a correlation between concentration and current was observed. A portable, handheld potentiostat assembled with Bluetooth communication and battery operation enables the developed system for point-of-care applications. A sandwich-like structure containing the sensing mechanisms as a prototype was designed to secure the biosensor to skin and use capillary action to draw sweat or other fluids toward the sensing mechanism. Overall, the immunosensor shows remarkable specificity, sensitivity as well as the noninvasive and point-of-care capabilities and allows the biosensor to be used as a versatile sensing platform in both developed and developing countries.展开更多
Monitoring the concentration of antibiotics in body fluids is essential to optimizing the therapy and minimizing the risk of bacteria resistance,which can be made with electrochemical sensors tailored with appropriate...Monitoring the concentration of antibiotics in body fluids is essential to optimizing the therapy and minimizing the risk of bacteria resistance,which can be made with electrochemical sensors tailored with appropriate materials.In this paper,we report on sensors made with screen-printed electrodes(SPE)coated with fullerene(C60),reduced graphene oxide(rGO)and Nafion(NF)(C60-rGO-NF/SPE)to determine the antibiotic metronidazole(MTZ).Under optimized conditions,the C60-rGO-NF/SPE sensor exhibited a linear response in square wave voltammetry for MTZ concentrations from 2.5×10^(-7) to 34×10^(-6) mol/L,with a detection limit of 2.1×10^(-7) mol/L.This sensor was also capable of detecting MTZ in serum and urine,with recovery between 94%and 100%,which are similar to those of the standard chromatographic method(HPLC-UV).Because the C60-rGO-NF/SPE sensor is amenable to mass production and allows for MTZ determination with simple principles of detection,it fulfills the requirements of therapeutic drug monitoring programs.展开更多
The electrochemical oxidation behavior and voltammetric assay of gemifloxacin were investigated using differential-pulse and cyclic voltammetry on a screen-printed carbon electrode.The effects of pH,scan rates,and con...The electrochemical oxidation behavior and voltammetric assay of gemifloxacin were investigated using differential-pulse and cyclic voltammetry on a screen-printed carbon electrode.The effects of pH,scan rates,and concentration of the drug on the anodic peak current were studied.Voltammograms of gemifloxacin in Tris-HCl buffer（pH 7.0） exhibited a well-defined single oxidation peak.A differential-pulse voltammetric procedure for the quantitation of gemifloxacin has been developed and suitably validated with respect to linearity,limits of detection and quantification,accuracy,precision,specificity,and robustness.The calibration was linear from 0.5 to 10.0 μM,and the limits of detection and quantification were 0.15 and 5.0 μM.Recoveries ranging from 96.26% to 103.64% were obtained.The method was successfully applied to the determination of gemifloxacin in pharmaceutical tablets without any pre-treatment.Excipients present in the tablets did not interfere in the assay.展开更多
An amperometric biosensor based on screen-printed electrodes （SPEs） was developed for the determination of organophosphorus pesticides in water samples. The extent of acetylcholinesterase （AChE） deactivation was d...An amperometric biosensor based on screen-printed electrodes （SPEs） was developed for the determination of organophosphorus pesticides in water samples. The extent of acetylcholinesterase （AChE） deactivation was determined and quantified for pesticide concentrations in water samples. An enzyme immobilization adsorption procedure and polyacrylamide gel matrix polymerization were used for fabrication of the biosensor, with minimal losses in enzyme activity. The optimal conditions for enzyme catalytic reaction on the SPEs surfaces were acetylthiocholine chloride （ATChCl） concentration of 5 mmol/L, pH 7 and reaction time of 4 min. The detection limits for three organophosphorus pesticides （dichlorvos, monocrotophs and parathion） were in the range of 4 to 7 μg/L when an AChE amount of 0.1 U was used for immobilization.展开更多
The screen-printed nanoporous TiO2 thin film was employed to fabricate dye-sensitized solid-state solar cells using CuI as hole-transport materials. The solar cell based on nanoporous TiO2 thin film with large pores f...The screen-printed nanoporous TiO2 thin film was employed to fabricate dye-sensitized solid-state solar cells using CuI as hole-transport materials. The solar cell based on nanoporous TiO2 thin film with large pores formed by the addition of polystyrene balls with diameter of 200 nm to the TiO2 paste exhibits photovoltaic performance enhancement, which is attributed to the good contact of CuI with surface of dye-sensitized thin film due to easy penetration of CuI in the film with large pores.展开更多
We report the fabrication of disposable and flexible Screen-Printed Electrodes (SPEs). This new type of screen-printed electrochemical platform consists of Ag nanoparticles (AgNPs) and graphite composite. For this pur...We report the fabrication of disposable and flexible Screen-Printed Electrodes (SPEs). This new type of screen-printed electrochemical platform consists of Ag nanoparticles (AgNPs) and graphite composite. For this purpose, silver nanoparticles were first synthesized by a chemical reduction method. The morphology and structure of the AgNPs were analyzed using a Scanning Electron Microscope (SEM) and UV-Visible spectroscopy. Graphite was chosen as the working electrode material for the fabrication of a thick-film. The fabrication of a screen-printed hydrogen peroxide biosensor consisting of three electrodes on a polyethylene terephthalate (PET) substrate was performed with a spraying approach (working, counter and reference: enzyme electrode, graphite, pseudo reference: Ag/AgCl). This biosensor was fabricated by immobilizing the peroxidase enzyme (HRP) in a Titania sol-gel membrane which was obtained through a vapor deposition method. The biosensor had electrocatalytic activity in the reduction of H2O2 with linear dependence on H2O2 concentration in the range of 10-5 to 10-3 M;the detection limit was 4.5 × 10-6 M.展开更多
In this study, gold nanoparticles and thermochromic composite films modified screen-printed carbon electrodes (TM-AuNPsSPCEs) were developed as a platform for the simultaneous detection of protein and temperature. The...In this study, gold nanoparticles and thermochromic composite films modified screen-printed carbon electrodes (TM-AuNPsSPCEs) were developed as a platform for the simultaneous detection of protein and temperature. The TM-AuNPs composited film had better sensitivity resulting from the gold nanoparticles amplification effect. A phase transition model analysis of TM-AuNPs films found that the TM-AuNPs films had three-phase transition intervals (<45℃, 45℃ to 80℃ and >80℃) which accommodated the temperature requirements for protein denaturation. When used to detect different concentrations of haemoglobin (Hb) solution, the TM-AuNPs modified SPCEs had a better sensitivity in detecting the different concentrations in comparison to TM and AuNP modified SPCEs which showed no clear sensitivity towards the different Hb concentrations. The dual detection and excellent sensitivity show a good application prospect for the study of the TM-AuNPs composite film.展开更多
Supercapacitor diode is a novel ion device that performs both supercapacitor energy storage and ion diode rectification functions.However,previously reported devices are limited by their large size and complex process...Supercapacitor diode is a novel ion device that performs both supercapacitor energy storage and ion diode rectification functions.However,previously reported devices are limited by their large size and complex processes.In this work,we demonstrate a screen-printed micro supercapacitor diode(MCAPode)that based on the insertion of a finger mode with spinel ZnCo_(2)O_(4) as cathode and activated carbon as anode for the first time,and featuring an excellent area specific capacitance(1.21 mF cm^(-2)at 10 mV s^(-1))and high rectification characteristics(rectification ratioⅠof 11.99 at 40 mV s^(-1)).Taking advantage of the ionic gel electrolyte,which provides excellent stability during repeated flexing and at high temperatures.In addition,MCAPode exhibits excellent electrochemical performance and rectification capability in"AND"and"OR"logic gates.These findings provide practical solutions for future expansion of micro supercapacitor diode applications.展开更多
The large-scale touch position sensor as a key human-machine interface toolkit holds immense significance in smart city and home construction.However,prior alternatives suffer from high power consumption,material limi...The large-scale touch position sensor as a key human-machine interface toolkit holds immense significance in smart city and home construction.However,prior alternatives suffer from high power consumption,material limitations,and implementation costs.Herein,a self-powered and scalable touch position strategy that integrates contact electrification with a screen-printing technique is proposed.Simply,high-impedance electrodes with stagger patterns are screen-printed onto various substrates before being covered with a dielectric layer.The locating mechanism originates from the touch-generated triboelectric charge shunt effect in the electrodes.The screen-printing parameters that affect the positional accuracy are discussed in detail.Leveraging this strategy,we realize a tailorable and large-scale triboelectric touch position sensor(LTTPS)that offers flexibility,self-powered capability,and a minimized signal channel,making it suitable for various practical scenarios.Demonstrations include an intelligent bookshelf mat with book management functionality,a rollable and foldable film-like keyboard,and a 4 m2 walk-tracking carpet.The LTTPS in this work provides an appealing alternative for large-scale touch positioning and enriches human-machine interaction.展开更多
The goal of this work is aimed to improve the power conversion efficiency of single crystalline silicon-based photovoltaic （PV） cells by using the solar spectral conversion principle, which employed a down-convertin...The goal of this work is aimed to improve the power conversion efficiency of single crystalline silicon-based photovoltaic （PV） cells by using the solar spectral conversion principle, which employed a down-converting phosphor to convert a high-energy ultraviolet photon to the less energetic red-emitting photons to improve the spectral response of Si solar cells. In this study, the surface of silicon solar cells was coated with a red-emitting KCaGd（PO4）2：Eu3＋ phosphor by using the screen-printing technique. In addition to the investigation on the microstructure using scanning electron microscopy （SEM）, we measured the short circuit current （Isc）, open circuit voltage （Voc）, and power conversion efficiency （η） of spectral-conversion cells and compared with those of bare solar cells as a reference. Preliminary experimental results revealed that in an optimized PV cell, an enhancement of （0.64＋0.01）% （from 16.03% to 16.67%） in Δη of a Si-based PV cell was achieved.展开更多
Point-of-care testing(POCT)technology is highly desirable for clinical diagnosis,healthcare monitoring,food safety inspection,and environment surveillance,because it enables rapid detection anywhere,anytime,and by any...Point-of-care testing(POCT)technology is highly desirable for clinical diagnosis,healthcare monitoring,food safety inspection,and environment surveillance,because it enables rapid detection anywhere,anytime,and by anyone.Electrochemiluminescence(ECL)has been widely used in chemo-/bio analysis due to its advantages such as high sensitivity,simplicity,rapidity and easy to control,and is now attracting increasing attention for POCT applications.However,to realize the accurate on-site quantitation,it is still challenging to develop portable devices which can precisely collect,analyze,transmit and display the ECL signals.This review will focus on how to develop a portable ECL device by summarizing recent examples and analyzing their key components part by part.Then the possible solutions to the existing challenges in the development and applications of portable ECL devices are summarized and discussed in detail,followed by offering future perspectives.We attempted to provide an appealing viewpoint to inspire interested researchers to comprehend and explore portable ECL sensing systems for practical applications and even commercialization.展开更多
An amperometric biosensor based on acetylcholinesterase (ACHE) is assembled by simple adsorption of the AChE on 7,7,8,8-tetracyanoquinodimethane (TCNQ) modified screen-printed electrodes.This biosensor is used to dete...An amperometric biosensor based on acetylcholinesterase (ACHE) is assembled by simple adsorption of the AChE on 7,7,8,8-tetracyanoquinodimethane (TCNQ) modified screen-printed electrodes.This biosensor is used to detect the inhibitory effect of organophosphorus pesticides on AChE activity.The detection of organophosphorus pesticides is done with acetylthiocboline chloride (ATCh) as substrate.In order to obtain the optimized response to substrate,the influencing factors of the biosensor are investigated,including temperature,pH,incubation time,substrate concentration and AChE concentration. The measurements were performed after inhibition by immersing the enzyme electrode into the parathion methyl solution. Under the optimized conditions,that is,500 U/ml AChE concentration,pH 7.2,10 min incubation time,2 mmol/L substrate concentration and temperature of 37℃,from 5×10^(-8) mol/L to 5×10^(-5) mol/L is close to linear (regression equation: y(%)=124.055+15.7991gx,R^2=0.99644),which corresponds to 8.7%～56.1% AChE inhibition.With the optimized conditions, the lowest detectable amount of parathion methyl is 13×10^(-9).展开更多
A rapid,simple,disposable and inexpensive acetylcholinesterase (ACHE) amperometric biosensor for the detection of organophosphorus pesticides was developed by simple adsorption of the enzyme on screen-printed electrod...A rapid,simple,disposable and inexpensive acetylcholinesterase (ACHE) amperometric biosensor for the detection of organophosphorus pesticides was developed by simple adsorption of the enzyme on screen-printed electrodes.The biosensor consisted of an Ag/AgCl reference electrode and a graphite working electrode.The mixture of graphite and the 7,7,8,8-tetracyanoquinodimethane (TCNQ) was printed on electrodes.The detection of organophosphorus pesticides was done with acetylthiocholine chloride (ATCh) as substrate.The biosensor was used to detect the inhibitory effect of organophosphorus pesticides on AChE activity.The 1μl of enzyme solution containing 0.1 U AChE and 1% bovine serum albumin (BSA) were simply dropped on the working electrode surface.The biosensor operated at a potential of 300 mV vs. Ag/AgCl in a pH 7.2 0.1 mol/L phosphate buffer and 0.1 mol/L KCl.We obtained a calibration plot of the percentage inhibition versus the logarithm of parathion methyl concentration following an incubation time of 10 mix in parathion methyl solution. The lowest detectable amount of parathion methyl was 0.026 ppm.The amperometric biosensor based on acetylcholinesterase was disposable and low cost (about 1 yuan RMB).展开更多
In this work,a solely gravity and capillary force-driven flow chemiluminescence(GCF-CL)paper-based microfuidic device has been proved for the first time as a new platforn for inex-pensive,usable,mini mally instrumente...In this work,a solely gravity and capillary force-driven flow chemiluminescence(GCF-CL)paper-based microfuidic device has been proved for the first time as a new platforn for inex-pensive,usable,mini mally instrumented dynamic chemiluninescence(CL)detection of chromium(Ⅲ)[Cr(Ⅲ)],where an appropriate angle of inclination between the loading and detection zones on the paper produces a rapid flow of CL prompt solution through the paper charnel.For this study,we use a cost-effective paper device that is manufactured by a simple wax screen-printing method,while the signal generated from the Cr(Ⅲ)-catalyzed oxidation of luminol by H_(2)O_(2) is recorded by a low-cost and luggable CCD camera.A series of GCF-CL affecting factors have been evaluated carefully.At optimal conditions,two linear relationships between GCF-CL intensities and the logarithms of Cr(Ⅲ)concentrations are obtained in the concentration mnges of 0.025-35 mg/L and 50-500 mg/L separately,with the detection limit of 0.0245mg/L for a les than 30s assay,and relative standard deviations(RSDs)of 38%,4.5%and 2.3%for 0.75,5 and 50 mg/L of Cr(Ⅲ)(n=8).The above results indicate that the GCF-CL paper-based microfluidic device possesses a receivable sensitivity,dynamic range,storage stability and reproducibility.Finally,the developed GCF-CL is utilized for Cr(Ⅲ)detection in real water samples.展开更多
Glass-ceramics are often used as sealants in solid oxide fuel cells (SOFC). But interfacing components, such as ferritic stainless steel and YSZ electrolyte, may vary in their requirements regarding sealing properties...Glass-ceramics are often used as sealants in solid oxide fuel cells (SOFC). But interfacing components, such as ferritic stainless steel and YSZ electrolyte, may vary in their requirements regarding sealing properties, especially in terms of thermal expansion. A bilayered glass-ceramic system was developed to overcome the mismatch in coefficients of thermal expansion (CTE) between ferritic steel and YSZ. Therefore, two different glass-ceramics with slightly different CTEs were developed, one with good bonding characteristics to the ferritic steel and the other to the YSZ electrolyte. Steel and electrolyte components were coated with a layer of their corresponding glass sealant paste and heated up to form a sandwich sample. During the heat treatment of the sealing process, the glasses are crystallized into glass-ceramics. The resulting interface between the two glass-ceramics is of special interest. Cross-sections of the sandwich samples were cut, polished and investigated using SEM. The glass-ceramics show continuous, gap-free layers and excellent bonding to both steel and YSZ. Energy release rates are measured for single and bilayered glass sealants by mechanical testing. The designed bilayered glass-ceramics fulfill the special requirements of ferritic steel and YSZ. They show excellent potential to become a new outstanding sealant for SOFCs.展开更多
基金supported by National Research Council of Thailand NRCT (grant number: N41A640129), Prince of Songkla University, Hat Yai, Thailandthe Talent Management Project of Prince of Songkla Universitythe Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ministry of Higher Education, Science, Research, and Innovation (MHESI)。
文摘Developing flexible bioelectronics is essential to the realization of artificial intelligence devices and biomedical applications, such as wearables, but their potential is limited by sustainable energy supply. An enzymatic biofuel cell(BFC) is promising for power supply, but its use is limited by the challenges of incorporating multiple enzymes and rigid platforms. This paper shows the first example of screen-printable nanocomposite inks engineered for a single-enzyme-based energy-harvesting device and a self-powered biosensor driven by glucose on bioanode and biocathode. The anode ink is modified with naphthoquinone and multiwalled carbon nanotubes(MWCNTs), whereas the cathode ink is modified with Prussian blue/MWCNT hybrid before immobilizing with glucose oxidase. The flexible bioanode and the biocathode consume glucose. This BFC yields an open circuit voltage of 0.45 V and a maximum power density of 266 μW cm-2. The wearable device coupled with a wireless portable system can convert chemical energy into electric energy and detect glucose in artificial sweat. The self-powered sensor can detect glucose concentrations up to 10 mM. Common interfering substances,including lactate, uric acid, ascorbic acid, and creatinine, have no effect on this self-powered biosensor. Additionally, the device can endure multiple mechanical deformations. New advances in ink development and flexible platforms enable a wide range of applications, including on-body electronics, self-sustainable applications, and smart fabrics.
基金Funded by Cofoe Medical Technology Co.,Ltd and the Scientific Research Start-up Funds of Hexi University(No.KYQD2022006)。
文摘CuO nanoparticles were successfully synthesized via a two-jet electrospun method,and then screen-printed on silver-carbon electrodes,forming CuO-modified Ag-C(CuO/Ag-C)disposable strip electrodes.In natural environment condition for glucose detection,the obtained CuO/Ag-C electrodes show a high sensitivity of 540 nA·mM^(-1)·cm^(-2),and a low limit of detection(0.68 mM)in a wide linear response range of 0.68 mM and 3 mM(signal/noise=3),respectively.In addition,the CuO/Ag-C electrodes also exhibit excellent anti-interference,air stability and repeatability.As a result,the fabrication of CuO nanoparticles via an electrospun process and the technique of screen-printed electrodes are of great significance for glucose detection.
基金supported by the National Natural Science Foundation of China(No.22174055)Key R&D Program of Zhenjiang City(No.NY2022012)。
文摘Wearable flexible sensor devices have the characteristics of lightweight and miniaturization.Currently,power supply and detection components limit the portability of wearable flexible sensor devices.Meanwhile,conventional liquid electrolytes are unsuitable for the integration of sensing devices.To address these constraints,wearable biofuel cells and flexible electrochromic displays have been introduced,which can improve integration with other devices,safety,and color-coded display data.Meanwhile,electrode chips prepared through screen printing technology can further improve portability.In this work,a wearable sensor device with screen-printed chips was constructed and used for non-invasive detection of glucose.Agarose gel electrolytes doped with PDA-CNTs were prepared,and the mechanical strength and moisture retention were significantly improved compared with traditional gel electrolytes.Glucose in interstitial fluid was non-invasive extracted to the skin surface using reverse iontophoresis.As a biofuel for wearable biofuel cells,glucose drives self-powered sensor and electrochromic display to produce color change,allowing for visually measurement of glucose levels in body fluids.Accurate detection results can be visualized by reading the RGB value with a cell phone.
文摘Even in small concentrations,toxic metals like lead,cadmium,and mercury are dangerous to the environment and human health.Environmental monitoring depends on precisely identifying these heavy metals,particularly cadmium ions(Cd(Ⅱ)).In this study,we present a novel screen-printed carbon electrode(SPCE)modified with single crystallineα-Fe_(2)O_(3)nano-hexagons that functions as a sensor for detecting Cd(Ⅱ).The performance of the fabricated sensor was thoroughly assessed and compared with unmodified SPCE using the voltammetric method.The crystalline structure of the synthesizedα-Fe_(2)O_(3)nano-hexagons was confirmed through XRD,and surface analysis revealed an average diameter and thickness of 86 nm and 9 nm,respectively.Theα-Fe_(2)O_(3)modified SPCE yields a 7-fold enhanced response(at pH 5.0 vs.Ag/AgCl)to Cd(Ⅱ)than bare SPCE.The modified electrode effectively detects Cd(Ⅱ)with a linear response range of up to 333.0μmol/L and a detection limit of 0.65 nmol/L under ideal circumstances.This newly fabricated sensor offers significant potential for environmental monitoring applications by providing outstanding practicality,anti-interference ability,and repeatability for detecting Cd(Ⅱ)in water samples.
基金financially supported by the Natural Science Foundation of Zhejiang Province(No.LQ17B050002)Analysis and Measurement Foundation of Zhejiang Province(No.2015C37068)
文摘A novel electrochemical method for the rapid detection of organophosphorus pesticide residues was realized on a dual-channel screen-printed electrode （DSPE） that was integrated with a portable smartphone-controlled potentiostat. The two carbon working channels of DSPE were first modified by electrodepositing of Prussian blue. The channels were then modified with acetylcholinesterase （ACHE） via Nation. The inhibition ratio of AChE was detected by comparing the electrical current of acetylthiocholine （ATCh） that was catalyzed by the enzyme electrodes with （channel 1） and without （channel 2） organophosphorus pesticide. Inhibition ratios were related with the negative logarithm of the organophosphorus pesticide （trichlorfon, oxamyl, and isocarbophos） concentrations at optimum experimental conditions （pH 6.9 of electrolyte, 0.2V working potential, 2.5μL AChE modification amount, and 15 min inhibition time）. The linear equations were 1%=32.301gC＋ 253.3 （R=0.9750） for isocarbophos, I% = 35.991gC＋ 270.1 （R = 0.9668） for chlorpyrifos, and 1% = 33.701gC＋ 250.5 （R = 0.9606） for trichlorfon. The detection limits were calculated as 10-7 g/mL. Given that the inhibition ratios were only related with pesticide concentration and not with pesticide species, the proposed electrodes and electrometer can rapidly detect universal organophosphorus pesticides and assess pesticide pollution.
基金the Natural Sciences and Engineering Research Council of Canada (400705) for funding this study
文摘A sensitive and specific immunosensor for the detection of the hormones cortisol and lactate in human or animal biological fluids, such as sweat and saliva, was devised using the label-free electrochemical chronoamperometric technique. By using these fluids instead of blood,the biosensor becomes noninvasive and is less stressful to the end user, who may be a small child or a farm animal.Electroreduced graphene oxide(e-RGO) was used as a synergistic platform for signal amplification and template for bioconjugation for the sensing mechanism on a screenprinted electrode. The cortisol and lactate antibodies were bioconjugated to the e-RGO using covalent carbodiimide chemistry. Label-free electrochemical chronoamperometric detection was used to analyze the response to the desired biomolecules over the wide detection range. A detection limit of 0.1 ng mL^(-1) for cortisol and 0.1 mM for lactate was established and a correlation between concentration and current was observed. A portable, handheld potentiostat assembled with Bluetooth communication and battery operation enables the developed system for point-of-care applications. A sandwich-like structure containing the sensing mechanisms as a prototype was designed to secure the biosensor to skin and use capillary action to draw sweat or other fluids toward the sensing mechanism. Overall, the immunosensor shows remarkable specificity, sensitivity as well as the noninvasive and point-of-care capabilities and allows the biosensor to be used as a versatile sensing platform in both developed and developing countries.
基金The authors gratefully acknowledge the financial support granted by CNPq,INEO,CAPES and FAPESP(Grant Nos.:2018/22214-6,2017/24053-7 and 2016/0991-5).
文摘Monitoring the concentration of antibiotics in body fluids is essential to optimizing the therapy and minimizing the risk of bacteria resistance,which can be made with electrochemical sensors tailored with appropriate materials.In this paper,we report on sensors made with screen-printed electrodes(SPE)coated with fullerene(C60),reduced graphene oxide(rGO)and Nafion(NF)(C60-rGO-NF/SPE)to determine the antibiotic metronidazole(MTZ).Under optimized conditions,the C60-rGO-NF/SPE sensor exhibited a linear response in square wave voltammetry for MTZ concentrations from 2.5×10^(-7) to 34×10^(-6) mol/L,with a detection limit of 2.1×10^(-7) mol/L.This sensor was also capable of detecting MTZ in serum and urine,with recovery between 94%and 100%,which are similar to those of the standard chromatographic method(HPLC-UV).Because the C60-rGO-NF/SPE sensor is amenable to mass production and allows for MTZ determination with simple principles of detection,it fulfills the requirements of therapeutic drug monitoring programs.
文摘The electrochemical oxidation behavior and voltammetric assay of gemifloxacin were investigated using differential-pulse and cyclic voltammetry on a screen-printed carbon electrode.The effects of pH,scan rates,and concentration of the drug on the anodic peak current were studied.Voltammograms of gemifloxacin in Tris-HCl buffer（pH 7.0） exhibited a well-defined single oxidation peak.A differential-pulse voltammetric procedure for the quantitation of gemifloxacin has been developed and suitably validated with respect to linearity,limits of detection and quantification,accuracy,precision,specificity,and robustness.The calibration was linear from 0.5 to 10.0 μM,and the limits of detection and quantification were 0.15 and 5.0 μM.Recoveries ranging from 96.26% to 103.64% were obtained.The method was successfully applied to the determination of gemifloxacin in pharmaceutical tablets without any pre-treatment.Excipients present in the tablets did not interfere in the assay.
基金supported by the National Natural Science Foundation of China (No. 51008026, 40873076 and 41011130204)
文摘An amperometric biosensor based on screen-printed electrodes （SPEs） was developed for the determination of organophosphorus pesticides in water samples. The extent of acetylcholinesterase （AChE） deactivation was determined and quantified for pesticide concentrations in water samples. An enzyme immobilization adsorption procedure and polyacrylamide gel matrix polymerization were used for fabrication of the biosensor, with minimal losses in enzyme activity. The optimal conditions for enzyme catalytic reaction on the SPEs surfaces were acetylthiocholine chloride （ATChCl） concentration of 5 mmol/L, pH 7 and reaction time of 4 min. The detection limits for three organophosphorus pesticides （dichlorvos, monocrotophs and parathion） were in the range of 4 to 7 μg/L when an AChE amount of 0.1 U was used for immobilization.
基金the financial support of this work by the Major State Basic Research Development Program(No.2006CB202605)High-Tech Research and Development of China Program(No.2007AA05Z439)+1 种基金the National Nature Science Foundation of China(No.50221201)Innovative Foundation of the Center for Molecular Science,Chinese Academy of Sciences(No.CMS-CX200718).
文摘The screen-printed nanoporous TiO2 thin film was employed to fabricate dye-sensitized solid-state solar cells using CuI as hole-transport materials. The solar cell based on nanoporous TiO2 thin film with large pores formed by the addition of polystyrene balls with diameter of 200 nm to the TiO2 paste exhibits photovoltaic performance enhancement, which is attributed to the good contact of CuI with surface of dye-sensitized thin film due to easy penetration of CuI in the film with large pores.
文摘We report the fabrication of disposable and flexible Screen-Printed Electrodes (SPEs). This new type of screen-printed electrochemical platform consists of Ag nanoparticles (AgNPs) and graphite composite. For this purpose, silver nanoparticles were first synthesized by a chemical reduction method. The morphology and structure of the AgNPs were analyzed using a Scanning Electron Microscope (SEM) and UV-Visible spectroscopy. Graphite was chosen as the working electrode material for the fabrication of a thick-film. The fabrication of a screen-printed hydrogen peroxide biosensor consisting of three electrodes on a polyethylene terephthalate (PET) substrate was performed with a spraying approach (working, counter and reference: enzyme electrode, graphite, pseudo reference: Ag/AgCl). This biosensor was fabricated by immobilizing the peroxidase enzyme (HRP) in a Titania sol-gel membrane which was obtained through a vapor deposition method. The biosensor had electrocatalytic activity in the reduction of H2O2 with linear dependence on H2O2 concentration in the range of 10-5 to 10-3 M;the detection limit was 4.5 × 10-6 M.
文摘In this study, gold nanoparticles and thermochromic composite films modified screen-printed carbon electrodes (TM-AuNPsSPCEs) were developed as a platform for the simultaneous detection of protein and temperature. The TM-AuNPs composited film had better sensitivity resulting from the gold nanoparticles amplification effect. A phase transition model analysis of TM-AuNPs films found that the TM-AuNPs films had three-phase transition intervals (<45℃, 45℃ to 80℃ and >80℃) which accommodated the temperature requirements for protein denaturation. When used to detect different concentrations of haemoglobin (Hb) solution, the TM-AuNPs modified SPCEs had a better sensitivity in detecting the different concentrations in comparison to TM and AuNP modified SPCEs which showed no clear sensitivity towards the different Hb concentrations. The dual detection and excellent sensitivity show a good application prospect for the study of the TM-AuNPs composite film.
基金the financial support from the Key Project of National Natural Science Foundation of China(12131010)the National Natural Science Foundation of China(22279166)+2 种基金the Special Project for Marine Economy Development of Guangdong Province(GDNRC[2023]26)the International Cooperation Base of Infrared Reflection Liquid Crystal Polymers and Device(2015B050501010)the Guangdong Basic and Applied Basic Research Foundation(2022B1515120019)。
文摘Supercapacitor diode is a novel ion device that performs both supercapacitor energy storage and ion diode rectification functions.However,previously reported devices are limited by their large size and complex processes.In this work,we demonstrate a screen-printed micro supercapacitor diode(MCAPode)that based on the insertion of a finger mode with spinel ZnCo_(2)O_(4) as cathode and activated carbon as anode for the first time,and featuring an excellent area specific capacitance(1.21 mF cm^(-2)at 10 mV s^(-1))and high rectification characteristics(rectification ratioⅠof 11.99 at 40 mV s^(-1)).Taking advantage of the ionic gel electrolyte,which provides excellent stability during repeated flexing and at high temperatures.In addition,MCAPode exhibits excellent electrochemical performance and rectification capability in"AND"and"OR"logic gates.These findings provide practical solutions for future expansion of micro supercapacitor diode applications.
基金National Key Research and Development Program,Grant/Award Number:2021YFA1201602NSFC,Grant/Award Numbers:T2422003,52302219Fundamental Research Funds for the Central Universities,Grant/Award Numbers:2024CDTZCQ-012,2024CDJGF-031。
文摘The large-scale touch position sensor as a key human-machine interface toolkit holds immense significance in smart city and home construction.However,prior alternatives suffer from high power consumption,material limitations,and implementation costs.Herein,a self-powered and scalable touch position strategy that integrates contact electrification with a screen-printing technique is proposed.Simply,high-impedance electrodes with stagger patterns are screen-printed onto various substrates before being covered with a dielectric layer.The locating mechanism originates from the touch-generated triboelectric charge shunt effect in the electrodes.The screen-printing parameters that affect the positional accuracy are discussed in detail.Leveraging this strategy,we realize a tailorable and large-scale triboelectric touch position sensor(LTTPS)that offers flexibility,self-powered capability,and a minimized signal channel,making it suitable for various practical scenarios.Demonstrations include an intelligent bookshelf mat with book management functionality,a rollable and foldable film-like keyboard,and a 4 m2 walk-tracking carpet.The LTTPS in this work provides an appealing alternative for large-scale touch positioning and enriches human-machine interaction.
基金Project supported by National Science Council of Taiwan (NSC98-2113-M-009-005-MY3)
文摘The goal of this work is aimed to improve the power conversion efficiency of single crystalline silicon-based photovoltaic （PV） cells by using the solar spectral conversion principle, which employed a down-converting phosphor to convert a high-energy ultraviolet photon to the less energetic red-emitting photons to improve the spectral response of Si solar cells. In this study, the surface of silicon solar cells was coated with a red-emitting KCaGd（PO4）2：Eu3＋ phosphor by using the screen-printing technique. In addition to the investigation on the microstructure using scanning electron microscopy （SEM）, we measured the short circuit current （Isc）, open circuit voltage （Voc）, and power conversion efficiency （η） of spectral-conversion cells and compared with those of bare solar cells as a reference. Preliminary experimental results revealed that in an optimized PV cell, an enhancement of （0.64＋0.01）% （from 16.03% to 16.67%） in Δη of a Si-based PV cell was achieved.
基金The financial support from the National Key Research and Development Program of China(No.2022YFE0201800)Shenzhen Science and Technology Innovation Commission(Nos.GJHZ20210705142200001 and JCYJ20210324140004013)Guangdong Natural Science Foundation(Nos.2021A1515220020 and 2020B1212060077)is gratefully acknowledged。
文摘Point-of-care testing(POCT)technology is highly desirable for clinical diagnosis,healthcare monitoring,food safety inspection,and environment surveillance,because it enables rapid detection anywhere,anytime,and by anyone.Electrochemiluminescence(ECL)has been widely used in chemo-/bio analysis due to its advantages such as high sensitivity,simplicity,rapidity and easy to control,and is now attracting increasing attention for POCT applications.However,to realize the accurate on-site quantitation,it is still challenging to develop portable devices which can precisely collect,analyze,transmit and display the ECL signals.This review will focus on how to develop a portable ECL device by summarizing recent examples and analyzing their key components part by part.Then the possible solutions to the existing challenges in the development and applications of portable ECL devices are summarized and discussed in detail,followed by offering future perspectives.We attempted to provide an appealing viewpoint to inspire interested researchers to comprehend and explore portable ECL sensing systems for practical applications and even commercialization.
文摘An amperometric biosensor based on acetylcholinesterase (ACHE) is assembled by simple adsorption of the AChE on 7,7,8,8-tetracyanoquinodimethane (TCNQ) modified screen-printed electrodes.This biosensor is used to detect the inhibitory effect of organophosphorus pesticides on AChE activity.The detection of organophosphorus pesticides is done with acetylthiocboline chloride (ATCh) as substrate.In order to obtain the optimized response to substrate,the influencing factors of the biosensor are investigated,including temperature,pH,incubation time,substrate concentration and AChE concentration. The measurements were performed after inhibition by immersing the enzyme electrode into the parathion methyl solution. Under the optimized conditions,that is,500 U/ml AChE concentration,pH 7.2,10 min incubation time,2 mmol/L substrate concentration and temperature of 37℃,from 5×10^(-8) mol/L to 5×10^(-5) mol/L is close to linear (regression equation: y(%)=124.055+15.7991gx,R^2=0.99644),which corresponds to 8.7%～56.1% AChE inhibition.With the optimized conditions, the lowest detectable amount of parathion methyl is 13×10^(-9).
文摘A rapid,simple,disposable and inexpensive acetylcholinesterase (ACHE) amperometric biosensor for the detection of organophosphorus pesticides was developed by simple adsorption of the enzyme on screen-printed electrodes.The biosensor consisted of an Ag/AgCl reference electrode and a graphite working electrode.The mixture of graphite and the 7,7,8,8-tetracyanoquinodimethane (TCNQ) was printed on electrodes.The detection of organophosphorus pesticides was done with acetylthiocholine chloride (ATCh) as substrate.The biosensor was used to detect the inhibitory effect of organophosphorus pesticides on AChE activity.The 1μl of enzyme solution containing 0.1 U AChE and 1% bovine serum albumin (BSA) were simply dropped on the working electrode surface.The biosensor operated at a potential of 300 mV vs. Ag/AgCl in a pH 7.2 0.1 mol/L phosphate buffer and 0.1 mol/L KCl.We obtained a calibration plot of the percentage inhibition versus the logarithm of parathion methyl concentration following an incubation time of 10 mix in parathion methyl solution. The lowest detectable amount of parathion methyl was 0.026 ppm.The amperometric biosensor based on acetylcholinesterase was disposable and low cost (about 1 yuan RMB).
基金supported by the National Natural Science Foundation of China(No.81571765)Guangzhou Science and Technology Program(No.2014J4100030)Guangdong Science and Technology Program(Nos.2014A020212503 and 2016A020215143).
文摘In this work,a solely gravity and capillary force-driven flow chemiluminescence(GCF-CL)paper-based microfuidic device has been proved for the first time as a new platforn for inex-pensive,usable,mini mally instrumented dynamic chemiluninescence(CL)detection of chromium(Ⅲ)[Cr(Ⅲ)],where an appropriate angle of inclination between the loading and detection zones on the paper produces a rapid flow of CL prompt solution through the paper charnel.For this study,we use a cost-effective paper device that is manufactured by a simple wax screen-printing method,while the signal generated from the Cr(Ⅲ)-catalyzed oxidation of luminol by H_(2)O_(2) is recorded by a low-cost and luggable CCD camera.A series of GCF-CL affecting factors have been evaluated carefully.At optimal conditions,two linear relationships between GCF-CL intensities and the logarithms of Cr(Ⅲ)concentrations are obtained in the concentration mnges of 0.025-35 mg/L and 50-500 mg/L separately,with the detection limit of 0.0245mg/L for a les than 30s assay,and relative standard deviations(RSDs)of 38%,4.5%and 2.3%for 0.75,5 and 50 mg/L of Cr(Ⅲ)(n=8).The above results indicate that the GCF-CL paper-based microfluidic device possesses a receivable sensitivity,dynamic range,storage stability and reproducibility.Finally,the developed GCF-CL is utilized for Cr(Ⅲ)detection in real water samples.
文摘Glass-ceramics are often used as sealants in solid oxide fuel cells (SOFC). But interfacing components, such as ferritic stainless steel and YSZ electrolyte, may vary in their requirements regarding sealing properties, especially in terms of thermal expansion. A bilayered glass-ceramic system was developed to overcome the mismatch in coefficients of thermal expansion (CTE) between ferritic steel and YSZ. Therefore, two different glass-ceramics with slightly different CTEs were developed, one with good bonding characteristics to the ferritic steel and the other to the YSZ electrolyte. Steel and electrolyte components were coated with a layer of their corresponding glass sealant paste and heated up to form a sandwich sample. During the heat treatment of the sealing process, the glasses are crystallized into glass-ceramics. The resulting interface between the two glass-ceramics is of special interest. Cross-sections of the sandwich samples were cut, polished and investigated using SEM. The glass-ceramics show continuous, gap-free layers and excellent bonding to both steel and YSZ. Energy release rates are measured for single and bilayered glass sealants by mechanical testing. The designed bilayered glass-ceramics fulfill the special requirements of ferritic steel and YSZ. They show excellent potential to become a new outstanding sealant for SOFCs.
