Objective: The aim of this work is to compare between intraoperative frozen section and scrape smear cytology in the diagnosis of ovarian neoplasm. Method: This study was performed between March 2011 and March 2012, o...Objective: The aim of this work is to compare between intraoperative frozen section and scrape smear cytology in the diagnosis of ovarian neoplasm. Method: This study was performed between March 2011 and March 2012, on 50 patients presented with ovarian mass. Gross examination of the tumor removed was done by inspection and palpation. The specimen was then cut with a sharp knife into two halves. The area was scraped with a sharp scalpel or the end of a glass slide, depending upon the type of tissue. A semifluid drop thus obtained was spread over a glass slide. One to four slides per case were taken from different representative areas. The slides were labelled and immediately put into 95% ethyl alcohol and stained with hematoxylin-eosin. The specimens were then fixed in formalin. Paraffin blocks of the sections were processed in the routine way and sections were stained with hematoxylin and eosin (H and E). Assessment of the overall accuracy of the intraoperative diagnosis was classified as concordant or discordant. Results: The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of scraping technique in the diagnosis of benign ovarian masses were 100%, 95.2%, 96.7%, 100% and 98% respectively. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of scraping technique in the diagnosis of border line ovarian masses were 100%, 93.4%, 25%, 100% and 94% respectively. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of scraping technique in the diagnosis of malignant ovarian masses were 80%, 100%, 100%, 88.2% and 92% respectively. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of frozen section in the diagnosis of benign ovarian masses were 100%, 100%, 100%, 100% and 100% respectively. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of frozen section in the diagnosis of borderline ovarian masses were 100%, 95.9%, 33.3%, 100% and 96% respectively. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of frozen section in the diagnosis of malignant ovarian masses were 90%, 100%, 100%, 93.8% and 96% respectively. Conclusion: Frozen section is more accurate than smear preparations in the intraoperative assessment of ovarian tumors in this study. However, the cytology preparations were helpful in supporting the histological diagnoses, and in some cases, provided additional useful information. Thus, cytology has a complementary role to frozen section in the intraoperative assessment of ovarian lesions. At the centers where the facilities of frozen section are not available, intraoperative scrape cytology is a useful tool for intraoperative diagnosis of tumor.展开更多
AIM:To explore the ocular features of corona virus disease(COVID)-19 and severe acute respiratory syndrome coronavirus(SARS-CoV)-2 detection in tears and conjunctival scrapes in non-severe COVID-19 patients.METHODS:Th...AIM:To explore the ocular features of corona virus disease(COVID)-19 and severe acute respiratory syndrome coronavirus(SARS-CoV)-2 detection in tears and conjunctival scrapes in non-severe COVID-19 patients.METHODS:This is a multicenter observational clinical study with no intervention conducted from Jan 25th to March 1st,2020.Clinical data and samples of tears and conjunctival scraping were collected in consecutive laboratory-confirmed,non-severe COVID-19 patients from three hospitals.COVID-19 virus was analyzed by real-time reverse transcriptase polymerase chain reaction(RT-PCR)kits.RESULTS:Totally 255 laboratory-confirmed,non-severe COVID-19 patients were recruited for ocular manifestation investigation.Of them,54.9%were females,with a mean age of 49.4 y.None of the patients has evidence of uveitis;11 patients(4.3%)complained of mild asthenopia;2(0.8%)had mild conjunctival congestion and serous secretion.Twenty-five of them had performed tears and conjunctival scrape for COVID-19 virus detection,with 4 yield possible positive results in the nucleoprotein gene.One of them were asymptomatic with normal chest CT and positive pharyngeal swab result.CONCLUSION:Ocular manifestations are neither common nor specific in non-severe COVID-19 patients.Meanwhile,COVID-19 virus nucleotides can be detected in the tears and conjunctival scrape samples,warranting further research on the transmissibility by the ocular route.展开更多
Scrap vulcanized rubber is amongst a bigger waste polymers. It does not decompose easily owing to its cross linked structure. Modification of scrap tires powder by the grafting with 2-acrylamido-2-methylpropanesulfoni...Scrap vulcanized rubber is amongst a bigger waste polymers. It does not decompose easily owing to its cross linked structure. Modification of scrap tires powder by the grafting with 2-acrylamido-2-methylpropanesulfonic acid (AMPS) is described. The grafting is achieved through free radical initiation. The effects of different amount of monomer and initiator were examined. Also the influence of reaction time and temperature were investigated. The grafted waste rubber was characterized by FT/IR, SEM and DSC measurements. The proposed mechanism of the grafting reaction is discussed. From DSC and SEM studies of WR-g-AMPS compared with PAMPS and WR, the results show that the particle size and crystallinity were enhanced for the grafted copolymers. The obtained modified scraped tires will used as an ion exchanger for the future applications.展开更多
OBJECTIVE:To reveal the effects of Guasha(scraping therapy) on the histomorphology of scraped skins and on the expressions of calcitonin gene-related peptide(CGRP) and substance P(SP).METHODS:50 rats,as experimental s...OBJECTIVE:To reveal the effects of Guasha(scraping therapy) on the histomorphology of scraped skins and on the expressions of calcitonin gene-related peptide(CGRP) and substance P(SP).METHODS:50 rats,as experimental subjects,were randomly divided into 5 groups according to different observation time points.Dorsal setae were shaved for the exposure of skin on both sides of the spine.Even reinforcing and reducing method was applied to the rats in Guasha groups on the site equivalent to bladder meridian of human body on one side of the spine,and the skin was scraped from top to bottom until rash of measles occurred.The skin tissues with rash of measles were taken down after perfusion.The corresponding sites of rats in group A were also taken down as control.The tissues were made to sections and used for immunofluorescence histochemical staining and HE staining of antibodies such as SP and CGRP.RESULTS:After Guasha,there were significant differences in appearance,hair follicle and blood vessel on local skin scraped on the back of rats when compared with control;In different time points,the differences reduced.There was no significant difference in expression of CGRP and SP when compared local skin scraped on the back of rats in different time points.CONCLUSION:Guasha didn't significantly change the morphology of nerve fibers inside local skin,and the histomorphology of hair follicle,blood vessel and etc.However,after Guasha they basically returned to normal level within five days.展开更多
目的:通过证据图谱系统梳理和展现刮痧干预颈肩腰腿痛临床研究的证据全貌,为临床和后续研究提供参考。方法:系统检索中国知网、万方、维普、中国生物医学文献服务系统、PubMed、Web of Science、Cochrane Library、Embase数据库中刮痧...目的:通过证据图谱系统梳理和展现刮痧干预颈肩腰腿痛临床研究的证据全貌,为临床和后续研究提供参考。方法:系统检索中国知网、万方、维普、中国生物医学文献服务系统、PubMed、Web of Science、Cochrane Library、Embase数据库中刮痧干预颈肩腰腿痛的随机对照试验相关文献,检索时限为建库至2024年11月10日,根据Cochrane系统评价手册对纳入文献进行方法学质量评价,采用图表结合的形式展现证据分布特点。结果:初次检索共获得5093篇文献,通过NoteExpress软件查重剔除3580篇重复文献,阅读题录剔除1032篇,阅读全文剔除251篇,最终纳入230篇文献,包括中文文献228篇,英文文献2篇,发文量总体呈波动上升趋势。聚类#0颈椎病、#1疼痛、#2艾灸、#3护理从出现年份一直延续至今,其中,聚类#0颈椎病跨度时间最长,从2003年一直持续到2024年。“铜砭刮痧”“疼痛”“温通刮痧”“功能障碍”是2021年、2022年的突现关键词,研究热度持续至今,“疼痛”突现强度最强。纳入的研究中共涉及17种病症,排名前3位的为颈椎病、腰椎间盘突出症、肩关节周围炎。在纳入文献的随机对照试验中,对疾病进行中医辨证分型的研究较少,仅有36篇,占15.65%,共涉及12种分型,以气滞血瘀型、寒湿痹阻型、肝肾不足型为主。刮痧工具以水牛角刮痧板最多(18.70%),其次为创新工具铜砭刮痧板(15.65%)、温通刮痧罐(13.04%)。应用方法以循经刮痧最多(58.26%),其次为穴位刮痧(22.61%)、全息理论(2.61%)。在纳入文献中,干预频次以每周1~2次最多,共124篇(53.91%),每周5~7次最少,共17篇(7.39%),有20篇文献未明确刮痧干预频率。结论:刮痧在干预颈肩腰腿痛的临床研究中应用广泛且具有一定疗效,刮痧工具与应用理论也在不断创新,但文献质量较差,存在样本量较小、干预频次与周期不一、忽略中医辨证分型、缺乏客观指标等缺点,未来需开展更多高质量、大样本、规范化的临床研究,为循证医学与临床干预提供更多参考。展开更多
文摘Objective: The aim of this work is to compare between intraoperative frozen section and scrape smear cytology in the diagnosis of ovarian neoplasm. Method: This study was performed between March 2011 and March 2012, on 50 patients presented with ovarian mass. Gross examination of the tumor removed was done by inspection and palpation. The specimen was then cut with a sharp knife into two halves. The area was scraped with a sharp scalpel or the end of a glass slide, depending upon the type of tissue. A semifluid drop thus obtained was spread over a glass slide. One to four slides per case were taken from different representative areas. The slides were labelled and immediately put into 95% ethyl alcohol and stained with hematoxylin-eosin. The specimens were then fixed in formalin. Paraffin blocks of the sections were processed in the routine way and sections were stained with hematoxylin and eosin (H and E). Assessment of the overall accuracy of the intraoperative diagnosis was classified as concordant or discordant. Results: The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of scraping technique in the diagnosis of benign ovarian masses were 100%, 95.2%, 96.7%, 100% and 98% respectively. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of scraping technique in the diagnosis of border line ovarian masses were 100%, 93.4%, 25%, 100% and 94% respectively. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of scraping technique in the diagnosis of malignant ovarian masses were 80%, 100%, 100%, 88.2% and 92% respectively. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of frozen section in the diagnosis of benign ovarian masses were 100%, 100%, 100%, 100% and 100% respectively. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of frozen section in the diagnosis of borderline ovarian masses were 100%, 95.9%, 33.3%, 100% and 96% respectively. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of frozen section in the diagnosis of malignant ovarian masses were 90%, 100%, 100%, 93.8% and 96% respectively. Conclusion: Frozen section is more accurate than smear preparations in the intraoperative assessment of ovarian tumors in this study. However, the cytology preparations were helpful in supporting the histological diagnoses, and in some cases, provided additional useful information. Thus, cytology has a complementary role to frozen section in the intraoperative assessment of ovarian lesions. At the centers where the facilities of frozen section are not available, intraoperative scrape cytology is a useful tool for intraoperative diagnosis of tumor.
基金Supported by the National Natural Science Foundation of China(No.81300798)Natural Science Foundation of Hunan Province(No.2018JJ3737)。
文摘AIM:To explore the ocular features of corona virus disease(COVID)-19 and severe acute respiratory syndrome coronavirus(SARS-CoV)-2 detection in tears and conjunctival scrapes in non-severe COVID-19 patients.METHODS:This is a multicenter observational clinical study with no intervention conducted from Jan 25th to March 1st,2020.Clinical data and samples of tears and conjunctival scraping were collected in consecutive laboratory-confirmed,non-severe COVID-19 patients from three hospitals.COVID-19 virus was analyzed by real-time reverse transcriptase polymerase chain reaction(RT-PCR)kits.RESULTS:Totally 255 laboratory-confirmed,non-severe COVID-19 patients were recruited for ocular manifestation investigation.Of them,54.9%were females,with a mean age of 49.4 y.None of the patients has evidence of uveitis;11 patients(4.3%)complained of mild asthenopia;2(0.8%)had mild conjunctival congestion and serous secretion.Twenty-five of them had performed tears and conjunctival scrape for COVID-19 virus detection,with 4 yield possible positive results in the nucleoprotein gene.One of them were asymptomatic with normal chest CT and positive pharyngeal swab result.CONCLUSION:Ocular manifestations are neither common nor specific in non-severe COVID-19 patients.Meanwhile,COVID-19 virus nucleotides can be detected in the tears and conjunctival scrape samples,warranting further research on the transmissibility by the ocular route.
文摘Scrap vulcanized rubber is amongst a bigger waste polymers. It does not decompose easily owing to its cross linked structure. Modification of scrap tires powder by the grafting with 2-acrylamido-2-methylpropanesulfonic acid (AMPS) is described. The grafting is achieved through free radical initiation. The effects of different amount of monomer and initiator were examined. Also the influence of reaction time and temperature were investigated. The grafted waste rubber was characterized by FT/IR, SEM and DSC measurements. The proposed mechanism of the grafting reaction is discussed. From DSC and SEM studies of WR-g-AMPS compared with PAMPS and WR, the results show that the particle size and crystallinity were enhanced for the grafted copolymers. The obtained modified scraped tires will used as an ion exchanger for the future applications.
基金the National Natural Science Foundation of China(General Program)(No.81173346No.30672720No.81674079)
文摘OBJECTIVE:To reveal the effects of Guasha(scraping therapy) on the histomorphology of scraped skins and on the expressions of calcitonin gene-related peptide(CGRP) and substance P(SP).METHODS:50 rats,as experimental subjects,were randomly divided into 5 groups according to different observation time points.Dorsal setae were shaved for the exposure of skin on both sides of the spine.Even reinforcing and reducing method was applied to the rats in Guasha groups on the site equivalent to bladder meridian of human body on one side of the spine,and the skin was scraped from top to bottom until rash of measles occurred.The skin tissues with rash of measles were taken down after perfusion.The corresponding sites of rats in group A were also taken down as control.The tissues were made to sections and used for immunofluorescence histochemical staining and HE staining of antibodies such as SP and CGRP.RESULTS:After Guasha,there were significant differences in appearance,hair follicle and blood vessel on local skin scraped on the back of rats when compared with control;In different time points,the differences reduced.There was no significant difference in expression of CGRP and SP when compared local skin scraped on the back of rats in different time points.CONCLUSION:Guasha didn't significantly change the morphology of nerve fibers inside local skin,and the histomorphology of hair follicle,blood vessel and etc.However,after Guasha they basically returned to normal level within five days.
文摘目的:通过证据图谱系统梳理和展现刮痧干预颈肩腰腿痛临床研究的证据全貌,为临床和后续研究提供参考。方法:系统检索中国知网、万方、维普、中国生物医学文献服务系统、PubMed、Web of Science、Cochrane Library、Embase数据库中刮痧干预颈肩腰腿痛的随机对照试验相关文献,检索时限为建库至2024年11月10日,根据Cochrane系统评价手册对纳入文献进行方法学质量评价,采用图表结合的形式展现证据分布特点。结果:初次检索共获得5093篇文献,通过NoteExpress软件查重剔除3580篇重复文献,阅读题录剔除1032篇,阅读全文剔除251篇,最终纳入230篇文献,包括中文文献228篇,英文文献2篇,发文量总体呈波动上升趋势。聚类#0颈椎病、#1疼痛、#2艾灸、#3护理从出现年份一直延续至今,其中,聚类#0颈椎病跨度时间最长,从2003年一直持续到2024年。“铜砭刮痧”“疼痛”“温通刮痧”“功能障碍”是2021年、2022年的突现关键词,研究热度持续至今,“疼痛”突现强度最强。纳入的研究中共涉及17种病症,排名前3位的为颈椎病、腰椎间盘突出症、肩关节周围炎。在纳入文献的随机对照试验中,对疾病进行中医辨证分型的研究较少,仅有36篇,占15.65%,共涉及12种分型,以气滞血瘀型、寒湿痹阻型、肝肾不足型为主。刮痧工具以水牛角刮痧板最多(18.70%),其次为创新工具铜砭刮痧板(15.65%)、温通刮痧罐(13.04%)。应用方法以循经刮痧最多(58.26%),其次为穴位刮痧(22.61%)、全息理论(2.61%)。在纳入文献中,干预频次以每周1~2次最多,共124篇(53.91%),每周5~7次最少,共17篇(7.39%),有20篇文献未明确刮痧干预频率。结论:刮痧在干预颈肩腰腿痛的临床研究中应用广泛且具有一定疗效,刮痧工具与应用理论也在不断创新,但文献质量较差,存在样本量较小、干预频次与周期不一、忽略中医辨证分型、缺乏客观指标等缺点,未来需开展更多高质量、大样本、规范化的临床研究,为循证医学与临床干预提供更多参考。
