The plant natural product scopolin,a coumarin secondary metabolite,has been extensively exploited in flavor,cosmetic,medicine,and other industrial fields.Melilotus albus,a leguminous rotation crop,contains high concen...The plant natural product scopolin,a coumarin secondary metabolite,has been extensively exploited in flavor,cosmetic,medicine,and other industrial fields.Melilotus albus,a leguminous rotation crop,contains high concentrations of coumarin.The transcriptional regulatory network that controls the flow through the scopolin biosynthesis pipeline in M.albus remains poorly understood.MabHLH11 encodes a basic helix–loop–helix(bHLH)transcription factor whose transcription is positively associated with scopolin accumulation and with the expression of MaMYB4,the bHLH partner of the MYB-bHLH complex.Phylogenetic analysis grouped MabHLH11 in the TRANSPARENT TESTA 8(TT8)clade of the bHLH IIIf subgroup.The MabHLH11 protein contained an MYB-interacting region and physically interacted with MaMYB4 in yeast and tobacco leaves.Co-overexpression of MabHLH11 with MaMYB4 in M.albus additively increased the expression of UDP-glucosyltransferase(MaUGT79)and induced more scopolin accumulation than occurred under the expression of MabHLH11 alone.MabHLH11 directly targeted the promoter of MaUGT79 and the activation of MabHLH11 was strengthened by the presence of MaMYB4.Thus,MaMYB4 enhanced the function of MabHLH11 in upregulating scopolin biosynthesis in M.albus,providing a theoretical basis for scalable production of a high-value plant natural product.展开更多
Objective To study the effects and mechanisms of scopolin isolated from the stems of Erycibe obtusifolia Benth in arthritis-associated inflammation and angiogenesis.Methods Adjuvant-induced arthritic rat,an animal mod...Objective To study the effects and mechanisms of scopolin isolated from the stems of Erycibe obtusifolia Benth in arthritis-associated inflammation and angiogenesis.Methods Adjuvant-induced arthritic rat,an animal model for human RA was used in this study for examining the potential remedial effect of scopolin.The swelling in both inoculated and non-inoculated paws,body weights and articular index(AI)scores were detected to evaluate the severity of the arthritis.Histologic assessment of tissue sections from rat ankles was also performed.Furthermore,the blood vessel density in the synovial tissues was quantitatively evaluated.In addition,expressions of VEGF,FGF-2,TNF-α,IL-1β and IL-6 in rat synovial tissues were determined by immunohistochemistry assay in an attempt to explain the mechanisms of scopolin for suppressing arthritis.Results Scopolin dose-dependently inhibited both inoculated and non-inoculated paw swelling in rat AIA.The mean AI scores of scopolin treated groups were also dose-dependently lower than that of model group.In addition,compared with the weights of model group,the mean body weights of rats treated with scopolin(50,100 mg·kg-1)were higher from day 13 to 22,perhaps indicative of healthier animals.The histologic architecture of the joint was highly abnormal in the model group rats,while high dose of scopolin treated rats preserved a nearly normal histologic architecture of the joint.Moreover,the new blood vessels were reduced dose-dependently in the synovial tissue of rat AIA treated with scopolin.Further,scopolin reduced the overexpression of IL-6,VEGF and FGF-2 in rat synovial tissues.Conclusions Scopolin is capable of reducing clinical symptoms of rat AIA by inhibiting inflammation and angiogenesis,and this compound may be a potent therapeutic agent for angiogenesis related diseases and can serve as structural base for screening for more potent synthetic analogs.展开更多
从新疆产毛节兔唇花[Lagochilus lanatonodus(C.Y.Wu et Hsuan)]中分离出8个化合物,经波谱鉴定分别为:β-sitosterol,Docosyl ester,Erythrodiol,Acetovanillone or apocynin,Androsin,Neolloydosin,Scopoletin和Scopolin,除β-sitoste...从新疆产毛节兔唇花[Lagochilus lanatonodus(C.Y.Wu et Hsuan)]中分离出8个化合物,经波谱鉴定分别为:β-sitosterol,Docosyl ester,Erythrodiol,Acetovanillone or apocynin,Androsin,Neolloydosin,Scopoletin和Scopolin,除β-sitosterol外,其它化合物均从该属植物中首次分到。展开更多
基金the National Natural Science Foundation of China(32061143035,32271752)Inner Mongolia Seed Industry Science and Technology Innovation Major Demonstration Project(2022JBGS0040)the Fundamental Research Funds for the Central Universities(lzujbky-2022-ey17).
文摘The plant natural product scopolin,a coumarin secondary metabolite,has been extensively exploited in flavor,cosmetic,medicine,and other industrial fields.Melilotus albus,a leguminous rotation crop,contains high concentrations of coumarin.The transcriptional regulatory network that controls the flow through the scopolin biosynthesis pipeline in M.albus remains poorly understood.MabHLH11 encodes a basic helix–loop–helix(bHLH)transcription factor whose transcription is positively associated with scopolin accumulation and with the expression of MaMYB4,the bHLH partner of the MYB-bHLH complex.Phylogenetic analysis grouped MabHLH11 in the TRANSPARENT TESTA 8(TT8)clade of the bHLH IIIf subgroup.The MabHLH11 protein contained an MYB-interacting region and physically interacted with MaMYB4 in yeast and tobacco leaves.Co-overexpression of MabHLH11 with MaMYB4 in M.albus additively increased the expression of UDP-glucosyltransferase(MaUGT79)and induced more scopolin accumulation than occurred under the expression of MabHLH11 alone.MabHLH11 directly targeted the promoter of MaUGT79 and the activation of MabHLH11 was strengthened by the presence of MaMYB4.Thus,MaMYB4 enhanced the function of MabHLH11 in upregulating scopolin biosynthesis in M.albus,providing a theoretical basis for scalable production of a high-value plant natural product.
文摘Objective To study the effects and mechanisms of scopolin isolated from the stems of Erycibe obtusifolia Benth in arthritis-associated inflammation and angiogenesis.Methods Adjuvant-induced arthritic rat,an animal model for human RA was used in this study for examining the potential remedial effect of scopolin.The swelling in both inoculated and non-inoculated paws,body weights and articular index(AI)scores were detected to evaluate the severity of the arthritis.Histologic assessment of tissue sections from rat ankles was also performed.Furthermore,the blood vessel density in the synovial tissues was quantitatively evaluated.In addition,expressions of VEGF,FGF-2,TNF-α,IL-1β and IL-6 in rat synovial tissues were determined by immunohistochemistry assay in an attempt to explain the mechanisms of scopolin for suppressing arthritis.Results Scopolin dose-dependently inhibited both inoculated and non-inoculated paw swelling in rat AIA.The mean AI scores of scopolin treated groups were also dose-dependently lower than that of model group.In addition,compared with the weights of model group,the mean body weights of rats treated with scopolin(50,100 mg·kg-1)were higher from day 13 to 22,perhaps indicative of healthier animals.The histologic architecture of the joint was highly abnormal in the model group rats,while high dose of scopolin treated rats preserved a nearly normal histologic architecture of the joint.Moreover,the new blood vessels were reduced dose-dependently in the synovial tissue of rat AIA treated with scopolin.Further,scopolin reduced the overexpression of IL-6,VEGF and FGF-2 in rat synovial tissues.Conclusions Scopolin is capable of reducing clinical symptoms of rat AIA by inhibiting inflammation and angiogenesis,and this compound may be a potent therapeutic agent for angiogenesis related diseases and can serve as structural base for screening for more potent synthetic analogs.
文摘从新疆产毛节兔唇花[Lagochilus lanatonodus(C.Y.Wu et Hsuan)]中分离出8个化合物,经波谱鉴定分别为:β-sitosterol,Docosyl ester,Erythrodiol,Acetovanillone or apocynin,Androsin,Neolloydosin,Scopoletin和Scopolin,除β-sitosterol外,其它化合物均从该属植物中首次分到。
