This study presented an improved CTAB method for extracting DNA from leaves of Populus euphratica Oliv. and Populus pruinosa Schrenk. based on the conventional CTAB method. The results showed that preventing DNA from ...This study presented an improved CTAB method for extracting DNA from leaves of Populus euphratica Oliv. and Populus pruinosa Schrenk. based on the conventional CTAB method. The results showed that preventing DNA from browning is a key step to obtain the high-quality DNA during DNA extraction, and under the condition of grinding in the presence of liquid nitrogen, adding such three antioxidants as PVP dry powder, Vc and β-mercaptoethanol could prevent DNA from browning effectively. The total DNA extracted by the improved CTAB method was subjected to PCR detection which proved that it totally satisfied the requirements of subsequent study.展开更多
The aims of this study were to explore the interspecific differences of Populus euphratica Oliv.and Populus pruinosa Schrenk populations and the intraspecific differences of males and females within the same species i...The aims of this study were to explore the interspecific differences of Populus euphratica Oliv.and Populus pruinosa Schrenk populations and the intraspecific differences of males and females within the same species in flowering phenological traits,and the effects of temperatures on flowering phenological traits in different growth years(2001–2003 and 2013–2015).The results showed that P.euphratica population flowered earlier than P.pruinosa population.Moreover,flowering phenological period of population,number of days of flowering phenological period per population,number of days of flowering phenological period per plant and average number of days of flowering period per plant of P.euphratica population were less than those of P.pruinosa population.The differences between male and female within the same species indicated that the flowering periods of males P.euphratica and P.pruinosa populations were earlier than those of female plants.For both species,flowering phenological traits were significantly and negatively correlated with the average temperatures in previous ten days,previous one month and previous three months of flowering.Both species are sensitive to temperature changes and adjust to the changes by advancing the start of flowering and prolonging the duration of flowering.展开更多
Global warming causes an unstable response in tree radial growth at high latitudes in the Northern Hemisphere.Additionally,different climatic responses of different age groups of trees have been found due to their dif...Global warming causes an unstable response in tree radial growth at high latitudes in the Northern Hemisphere.Additionally,different climatic responses of different age groups of trees have been found due to their different physiological mechanisms.In this study,the response stability and growth trend of three age groups(young<100 a,middle 100-200 a,old≥200 a)of Picea schrenkiana(Schrenk spruce)to climate change and the causes of the different responses in different age groups were analyzed in the relatively dry climate of the eastern Tianshan Mountains.The results showed that:(1)With the abrupt increase in temperature in 1989,the annual mean minimum temperature became the dominant radial growth-limiting factor of the three age groups of Schrenk spruce.(2)The radial growth of the middle and young groups was more sensitive than that of the old group based on growth-climate correlation analysis.(3)The radial growth of the different age groups had different responses to climate factors,and all age groups were unstable on time scales.(4)The trend of the linear regression simulation of the basal area increment(BAI)indicated that the Schrenk spruce had the same growth trends in different age groups with growth first increased and then decreased;however,the decreased growth rate was higher in the middle and young age groups than in the old age group after the abrupt increase in temperature.Therefore,we should pay active attention to the impact of drought on Schrenk spruce in the eastern Tianshan Mountains and should particularly strengthen the conservation and management of the middle and young age groups.展开更多
The dried roots of Berberis heteropoda Schrenk have traditionally been used to treat acute gastroenteritis and dysentery. The aim of this study was to confirm the antibacterial activity of an extract of Berberis heter...The dried roots of Berberis heteropoda Schrenk have traditionally been used to treat acute gastroenteritis and dysentery. The aim of this study was to confirm the antibacterial activity of an extract of Berberis heteropoda Schrenk root in vitro and its therapeutic effects on rats with diarrhea-predominant irritable bowel syndrome(D-IBS) in vivo, as well as to identify the related signaling pathways. A water extract of Berberis heteropoda Schrenk root(BHS) inhibited the growth of S. aureus, E. coli, P. aeruginosa and S. faecalis. BHS potentially damaged the structure of the bacterial cell membrane and decreased the activity of some membranous enzymes, eventually killing the S. aureus, E. coli, P. aeruginosa and S. faecalis bacteria. Oral administration of BHS(low, middle and high dose group, L, M and H) significantly alleviated the abdominal pain, diarrhea, and depression-like symptoms of D-IBS rats, and the efficacy index ranged from 30% to 60%, indicating that the BHS treatment was effective. BHS(L, M and H) alleviated the abnormal pathological changes in the brain, as evidenced by HE staining. The expression of CHAT, 5-HT, C-FOS and CGRP was reduced by the BHS treatment(L, M and H). Our findings provide novel insights into the use of the natural product BHS to inhibit pathogenic bacteria by destroying the bacterial structure, indicating that BHS possesses certain biological activities. Furthermore, BHS has the potential to alleviate diarrhea, abdominal pain and depression-like behaviors in D-IBS rats by regulating the brain-gut peptide levels.展开更多
To study the historical temperature variation in Yili of Xinjiang,the tree-ring chronologies of maximum density(MXD)and tree-ring width(TRW)were established with the Schrenk Spruce(Picea schrenkiana Fisch.et Mey)colle...To study the historical temperature variation in Yili of Xinjiang,the tree-ring chronologies of maximum density(MXD)and tree-ring width(TRW)were established with the Schrenk Spruce(Picea schrenkiana Fisch.et Mey)collected from six sites to analyze the characteristics and ability of response to climate change.The results suggest that the MXD chronology of the Schrenk Spruce from different sampling sites responded to climate change well and were positively correlated with the mean maximum temperature and the mean temperature from April to August in the study area.The mean maximum temperature anomaly from April to August,spanning the years from 1848 to 2000,was reconstructed by three MXD chronologies which were selected by stepwise regression.The reconstructed function was stable and explained 56.2%of the variance.The reconstructed results indicated a cold span of 153 years in spring and summer from the beginning of the 1950s to the beginning of the 1970s in the Yili valley.No strongly increasing tendency was detected in the mean maximum temperature anomaly from April to August during this reconstructed period.展开更多
全球气候变暖和大气CO_(2)浓度(Ca)急剧上升已成为不争的事实,并对森林生态系统有着深远的影响,全面理解森林生态系统对全球变化的响应至关重要。中亚干旱区特有树种雪岭云杉(Picea schrenkiana Fisch. et Mey)树轮稳定碳同位素(δ^(13...全球气候变暖和大气CO_(2)浓度(Ca)急剧上升已成为不争的事实,并对森林生态系统有着深远的影响,全面理解森林生态系统对全球变化的响应至关重要。中亚干旱区特有树种雪岭云杉(Picea schrenkiana Fisch. et Mey)树轮稳定碳同位素(δ^(13)C)和水分利用效率(iWUE)对气候变暖和大气CO2浓度急剧上升的响应缺乏研究。本研究利用天山西部伊犁河流域的雪岭云杉树轮样本,使用树木年代学方法和树轮稳定碳同位素技术,建立树轮宽度年表、稳定碳同位素(δ^(13)C)以及内禀水分利用效率(iWUE)序列,分析了树轮δ13C和iWUE的长期变化特征,探讨了树轮δ13C和iWUE对气候的响应规律以及iWUE与树轮宽度的关系。结果表明,主要的气候因子并没有强烈地限制树木径向生长;夏季平均气温对树轮δ^(13)C分馏有重要影响,iWUE的长期变化受到全球Ca增加和升温趋势影响,但直接影响iWUE年际变化的主要因素是饱和水汽压亏缺(VPD)。全球升温和Ca增加导致了iWUE的持续升高,但并没有导致雪岭云杉树木径向生长的明显增加。展开更多
基金Supported by Natural Science Foundation of China(31160110)Open Project of Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin(BRYB1003)the Principle Fund of Tarim University(TDZKSS201419)~~
文摘This study presented an improved CTAB method for extracting DNA from leaves of Populus euphratica Oliv. and Populus pruinosa Schrenk. based on the conventional CTAB method. The results showed that preventing DNA from browning is a key step to obtain the high-quality DNA during DNA extraction, and under the condition of grinding in the presence of liquid nitrogen, adding such three antioxidants as PVP dry powder, Vc and β-mercaptoethanol could prevent DNA from browning effectively. The total DNA extracted by the improved CTAB method was subjected to PCR detection which proved that it totally satisfied the requirements of subsequent study.
基金financially supported by the National Natural Sciences Foundation of China(30060007)the National Basic Research Program of China(2012CB723204)the National Programs for Science and Technology Development of China(2013BAC10B01)
文摘The aims of this study were to explore the interspecific differences of Populus euphratica Oliv.and Populus pruinosa Schrenk populations and the intraspecific differences of males and females within the same species in flowering phenological traits,and the effects of temperatures on flowering phenological traits in different growth years(2001–2003 and 2013–2015).The results showed that P.euphratica population flowered earlier than P.pruinosa population.Moreover,flowering phenological period of population,number of days of flowering phenological period per population,number of days of flowering phenological period per plant and average number of days of flowering period per plant of P.euphratica population were less than those of P.pruinosa population.The differences between male and female within the same species indicated that the flowering periods of males P.euphratica and P.pruinosa populations were earlier than those of female plants.For both species,flowering phenological traits were significantly and negatively correlated with the average temperatures in previous ten days,previous one month and previous three months of flowering.Both species are sensitive to temperature changes and adjust to the changes by advancing the start of flowering and prolonging the duration of flowering.
基金supported by the National Natural Science Foundation of China(Projects No.41861006 and 41630750)the Scientific Research Program of Higher Education Institutions of Gansu Province(2018C-02)the Research Ability Promotion Program for Young Teachers of Northwest Normal University(NWNU-LKQN2019-4)。
文摘Global warming causes an unstable response in tree radial growth at high latitudes in the Northern Hemisphere.Additionally,different climatic responses of different age groups of trees have been found due to their different physiological mechanisms.In this study,the response stability and growth trend of three age groups(young<100 a,middle 100-200 a,old≥200 a)of Picea schrenkiana(Schrenk spruce)to climate change and the causes of the different responses in different age groups were analyzed in the relatively dry climate of the eastern Tianshan Mountains.The results showed that:(1)With the abrupt increase in temperature in 1989,the annual mean minimum temperature became the dominant radial growth-limiting factor of the three age groups of Schrenk spruce.(2)The radial growth of the middle and young groups was more sensitive than that of the old group based on growth-climate correlation analysis.(3)The radial growth of the different age groups had different responses to climate factors,and all age groups were unstable on time scales.(4)The trend of the linear regression simulation of the basal area increment(BAI)indicated that the Schrenk spruce had the same growth trends in different age groups with growth first increased and then decreased;however,the decreased growth rate was higher in the middle and young age groups than in the old age group after the abrupt increase in temperature.Therefore,we should pay active attention to the impact of drought on Schrenk spruce in the eastern Tianshan Mountains and should particularly strengthen the conservation and management of the middle and young age groups.
基金grants from the National Natural Science Foundation of China (Nos.81760645,81860773,81873185 and U1703108)Xinjiang Science Fund for Distinguished Young Scholar Project (No.2018Q003)Natural Science Foundation of Jiangsu province (No.BK20181327)。
文摘The dried roots of Berberis heteropoda Schrenk have traditionally been used to treat acute gastroenteritis and dysentery. The aim of this study was to confirm the antibacterial activity of an extract of Berberis heteropoda Schrenk root in vitro and its therapeutic effects on rats with diarrhea-predominant irritable bowel syndrome(D-IBS) in vivo, as well as to identify the related signaling pathways. A water extract of Berberis heteropoda Schrenk root(BHS) inhibited the growth of S. aureus, E. coli, P. aeruginosa and S. faecalis. BHS potentially damaged the structure of the bacterial cell membrane and decreased the activity of some membranous enzymes, eventually killing the S. aureus, E. coli, P. aeruginosa and S. faecalis bacteria. Oral administration of BHS(low, middle and high dose group, L, M and H) significantly alleviated the abdominal pain, diarrhea, and depression-like symptoms of D-IBS rats, and the efficacy index ranged from 30% to 60%, indicating that the BHS treatment was effective. BHS(L, M and H) alleviated the abnormal pathological changes in the brain, as evidenced by HE staining. The expression of CHAT, 5-HT, C-FOS and CGRP was reduced by the BHS treatment(L, M and H). Our findings provide novel insights into the use of the natural product BHS to inhibit pathogenic bacteria by destroying the bacterial structure, indicating that BHS possesses certain biological activities. Furthermore, BHS has the potential to alleviate diarrhea, abdominal pain and depression-like behaviors in D-IBS rats by regulating the brain-gut peptide levels.
基金Supported by the National Basic Research Program of China(Grant No.2009CB421307)Research Foundation of Chinese Desert Meteorology(Grant No.SQJ2006013)National Natural Science Foundation of China(Grant Nos.30270227 and J0630965)
文摘To study the historical temperature variation in Yili of Xinjiang,the tree-ring chronologies of maximum density(MXD)and tree-ring width(TRW)were established with the Schrenk Spruce(Picea schrenkiana Fisch.et Mey)collected from six sites to analyze the characteristics and ability of response to climate change.The results suggest that the MXD chronology of the Schrenk Spruce from different sampling sites responded to climate change well and were positively correlated with the mean maximum temperature and the mean temperature from April to August in the study area.The mean maximum temperature anomaly from April to August,spanning the years from 1848 to 2000,was reconstructed by three MXD chronologies which were selected by stepwise regression.The reconstructed function was stable and explained 56.2%of the variance.The reconstructed results indicated a cold span of 153 years in spring and summer from the beginning of the 1950s to the beginning of the 1970s in the Yili valley.No strongly increasing tendency was detected in the mean maximum temperature anomaly from April to August during this reconstructed period.
文摘全球气候变暖和大气CO_(2)浓度(Ca)急剧上升已成为不争的事实,并对森林生态系统有着深远的影响,全面理解森林生态系统对全球变化的响应至关重要。中亚干旱区特有树种雪岭云杉(Picea schrenkiana Fisch. et Mey)树轮稳定碳同位素(δ^(13)C)和水分利用效率(iWUE)对气候变暖和大气CO2浓度急剧上升的响应缺乏研究。本研究利用天山西部伊犁河流域的雪岭云杉树轮样本,使用树木年代学方法和树轮稳定碳同位素技术,建立树轮宽度年表、稳定碳同位素(δ^(13)C)以及内禀水分利用效率(iWUE)序列,分析了树轮δ13C和iWUE的长期变化特征,探讨了树轮δ13C和iWUE对气候的响应规律以及iWUE与树轮宽度的关系。结果表明,主要的气候因子并没有强烈地限制树木径向生长;夏季平均气温对树轮δ^(13)C分馏有重要影响,iWUE的长期变化受到全球Ca增加和升温趋势影响,但直接影响iWUE年际变化的主要因素是饱和水汽压亏缺(VPD)。全球升温和Ca增加导致了iWUE的持续升高,但并没有导致雪岭云杉树木径向生长的明显增加。
