[Objective] The paper was to study the antagonistic effect of Russula virescens （Schaeff.） Ft. against Bortrytis cinerea. [Method] Using the confrontation culture method, the antagonistic effect of mycelium, ferment...[Objective] The paper was to study the antagonistic effect of Russula virescens （Schaeff.） Ft. against Bortrytis cinerea. [Method] Using the confrontation culture method, the antagonistic effect of mycelium, fermentation broth and mycelial extract of R. virescens against B. cinerea was studied. [Result] The antagonistic ef- fect of R. vivesscens against B. cinerea was mainly manifested as hyperparasitism of mycelium and inhibition effect of metabolites. The inhibition rate of confrontation growth was between 46.9% and 52.3%, and hyperparasitism phenomenon was obvi- ous. The fermentation broth of R. virescens cultured by PDB medium had the strongest growth inhibition effect, and the growth inhibition rate against B. cinerea was the highest of 61.59%. The thermal stability of fermentation broth was good, the mycelial extract of R. virescens had no inhibition effect against the growth of B. cinerea. [Conclusion] The study provided theoretical basis for subsequent differentiation and drug sensitivity of B. cinerea.展开更多
A new water-soluble heteropolysaccharide with a molecular weight of 15 k Da was isolated from the fruiting bodies of Boletus reticulatus Schaeff.Structural characterization results revealed that B.reticulatus Schaeff ...A new water-soluble heteropolysaccharide with a molecular weight of 15 k Da was isolated from the fruiting bodies of Boletus reticulatus Schaeff.Structural characterization results revealed that B.reticulatus Schaeff polysaccharide(BRS-X)had a backbone of 1,6-linkedα-D-galactose and 1,2,6-linkedα-D-galactose which branches were mainly composed of a terminal 4-linkedβ-D-glucose and the ratio of D-galactose and D-glucose was 5:1.Bioactivity assays indicated that BRS-X displayed a strong proliferative activity in T cells and B cells and promoted the secretion of immunoglobulin G(Ig G),Ig E,Ig D and Ig M.In addition,BRS-X could facilitate the proliferation and phagocytosis of RAW264.7 cells and could significantly inhibit the growth of tumors in S180-bearing mice.The results of transcriptome sequencing analysis illustrated that total 46 genes enriched in MAPK and total 34 genes enriched in PI3 K/Akt signaling pathways in BRS-X group.The protein VEGF and VEGFR expression were significantly reduced under the treatment with BRS-X.These findings provide a scientific basis for the edible and medicinal value of BRS-X.展开更多
[目的]优化野蘑菇三萜类化合物的最佳提取工艺。[方法]以野蘑菇菌种培养所得的菌丝体为原料,采用超声波辅助、异丙醇-硫酸铵双水相体系提取野蘑菇中的三萜类化合物,并建立检测野蘑菇中三萜类化合物的紫外分光光度法(UV法)。对影响三萜...[目的]优化野蘑菇三萜类化合物的最佳提取工艺。[方法]以野蘑菇菌种培养所得的菌丝体为原料,采用超声波辅助、异丙醇-硫酸铵双水相体系提取野蘑菇中的三萜类化合物,并建立检测野蘑菇中三萜类化合物的紫外分光光度法(UV法)。对影响三萜类化合物提取率的主要因素--异丙醇体积浓度、硫酸铵质量浓度、提取时间进行单因素试验,在此基础上根据Box-Benhnken试验设计原理利用响应面法对提取工艺进行优化。[结果]野蘑菇菌丝体内三萜类化合物的提取最佳工艺条件为异丙醇体积浓度45%,硫酸铵浓度0.175 g/mL,超声波提取时间35 min(320 W 4℃)。在该条件下,三萜提取率为最高。[结论]该研究为野蘑菇中三萜类化合物的提取及进一步开发利用奠定了基础。展开更多
基金Supported by Applied Foundation Project of Science and Technology Department of Sichuan Province (2008JY0021-2)~~
文摘[Objective] The paper was to study the antagonistic effect of Russula virescens （Schaeff.） Ft. against Bortrytis cinerea. [Method] Using the confrontation culture method, the antagonistic effect of mycelium, fermentation broth and mycelial extract of R. virescens against B. cinerea was studied. [Result] The antagonistic ef- fect of R. vivesscens against B. cinerea was mainly manifested as hyperparasitism of mycelium and inhibition effect of metabolites. The inhibition rate of confrontation growth was between 46.9% and 52.3%, and hyperparasitism phenomenon was obvi- ous. The fermentation broth of R. virescens cultured by PDB medium had the strongest growth inhibition effect, and the growth inhibition rate against B. cinerea was the highest of 61.59%. The thermal stability of fermentation broth was good, the mycelial extract of R. virescens had no inhibition effect against the growth of B. cinerea. [Conclusion] The study provided theoretical basis for subsequent differentiation and drug sensitivity of B. cinerea.
基金supported by the Open Project Program of Irradiation Preservation Technology Key Laboratory of Sichuan Province,Sichuan Institute of Atomic Energy(FZBC2020009)the Open Research Fund Program of Departmental and Municipal Co-construction of Crops Genetic Improvement of Hill Land Key Laboratory of Sichuan Province(2021CGIHL02)+2 种基金Science and Technology Support Project of Nanchong Science and Technology Bureau of Sichuan Province(20YFZJ0053 and 20YFZJ0054)the Sericulture Innovation Team of Sichuan Province(SCCXTD-2021-17)Laboratory of Sichuan Province(2021CGIHL02)。
文摘A new water-soluble heteropolysaccharide with a molecular weight of 15 k Da was isolated from the fruiting bodies of Boletus reticulatus Schaeff.Structural characterization results revealed that B.reticulatus Schaeff polysaccharide(BRS-X)had a backbone of 1,6-linkedα-D-galactose and 1,2,6-linkedα-D-galactose which branches were mainly composed of a terminal 4-linkedβ-D-glucose and the ratio of D-galactose and D-glucose was 5:1.Bioactivity assays indicated that BRS-X displayed a strong proliferative activity in T cells and B cells and promoted the secretion of immunoglobulin G(Ig G),Ig E,Ig D and Ig M.In addition,BRS-X could facilitate the proliferation and phagocytosis of RAW264.7 cells and could significantly inhibit the growth of tumors in S180-bearing mice.The results of transcriptome sequencing analysis illustrated that total 46 genes enriched in MAPK and total 34 genes enriched in PI3 K/Akt signaling pathways in BRS-X group.The protein VEGF and VEGFR expression were significantly reduced under the treatment with BRS-X.These findings provide a scientific basis for the edible and medicinal value of BRS-X.
文摘[目的]优化野蘑菇三萜类化合物的最佳提取工艺。[方法]以野蘑菇菌种培养所得的菌丝体为原料,采用超声波辅助、异丙醇-硫酸铵双水相体系提取野蘑菇中的三萜类化合物,并建立检测野蘑菇中三萜类化合物的紫外分光光度法(UV法)。对影响三萜类化合物提取率的主要因素--异丙醇体积浓度、硫酸铵质量浓度、提取时间进行单因素试验,在此基础上根据Box-Benhnken试验设计原理利用响应面法对提取工艺进行优化。[结果]野蘑菇菌丝体内三萜类化合物的提取最佳工艺条件为异丙醇体积浓度45%,硫酸铵浓度0.175 g/mL,超声波提取时间35 min(320 W 4℃)。在该条件下,三萜提取率为最高。[结论]该研究为野蘑菇中三萜类化合物的提取及进一步开发利用奠定了基础。
