[Objective] The research aimed to provide reference for classification of pomegranate cultivars and studies of genetic relationship among pomegranate cultivars.[Method] The electron microscope scanning was used to com...[Objective] The research aimed to provide reference for classification of pomegranate cultivars and studies of genetic relationship among pomegranate cultivars.[Method] The electron microscope scanning was used to comparatively observe leaf epidermal structures of 4 pomegranate cultivars.[Result] The upper epidermal structures of 4 pomegranate cultivars were similar and showed reticular structure .However, the differences existed in lower epidermis,such as cell shape,cell size and arrangement mode of cell as well as stomatal density,while the structures of leaf vein in lower epidermis of 4 pomegranate cultivars were similar.[Conclusion] The research provided morphological references for studying heterosis of pomegranate to some extent.展开更多
Changes of calmodulin (CaM) distribution in the embryo sac of rice (Oryza sativa subsp. Japonica) at various stages before and after fertilization have been investigated by using immunogold electron microscopy. Before...Changes of calmodulin (CaM) distribution in the embryo sac of rice (Oryza sativa subsp. Japonica) at various stages before and after fertilization have been investigated by using immunogold electron microscopy. Before pollination, both cytoplasm and vacuoles of the egg cell, synergids and central cell were labeled by gold particles. A small amount of gold particles were localized in the nucleus, endoplasmic reticulum, mitochondria and dictyosomes. From pollination to fertilization, CaM amount increased in these cells, especially rich in the starch of amyloplasts. Increase of gold particles in the central cell began about 2 h earlier than that in the egg cell. There was no distinct difference of CaM amount between the degenerated and the persistent synergids. It is interesting to observe an obvious change of CaM distribution form during pollination and fertilization from scattered single particles to clustered particles, and back again to single particles after the fertilization finished. CaM was also localized extracellularly in the embryo sac wall as well as in the wall and intercellular space of nucellus cells. The extracellular CaM also changes in its amount and form after pollination. These results suggest that CaM, either intra- or extra-cellular, may play important roles in fertilization and zygote formation.展开更多
[Objective]The experiment aimed to explore a new way for observing surface structure of Nostoc sphaeroides Kutzing. [Method] The scanning electron microscope was used to observe the epidermal ultrastructure of wild an...[Objective]The experiment aimed to explore a new way for observing surface structure of Nostoc sphaeroides Kutzing. [Method] The scanning electron microscope was used to observe the epidermal ultrastructure of wild and cultured Nostoc sphaeroides Kutzing. [ Result] The epidermis of wild and cultured Nostoc sphaeroides Kutzing showed mixture structure of fibril colloid which was reticular arranged. The difference between wild and cultured Nostoc sphaeroides Kutzing was that the outer epidermis of cultured Nostoc sphaeroides Kutzing had trichome distribution but the wild Nostoc sphaeroides Kutzing did not has such distribution. The obsevation results of under smaller than 10 μm by scanning electron microscope was touched thick and showed many folds and distortions. [ Conclusion] The scanning electron microscope was an effective way to study development of Nostoc sphaeroides Kutzing colony and it was worth popularizing.展开更多
[ Objective] The paper was to observe Helicotylenchus digonicus by scanning electron microscope （SEM）. [ Method ] H. digonicus collected from Changxing of Zhejiang Province was observed under scanning electron micro...[ Objective] The paper was to observe Helicotylenchus digonicus by scanning electron microscope （SEM）. [ Method ] H. digonicus collected from Changxing of Zhejiang Province was observed under scanning electron microscope, and its morphological structure was confmned under optical microscope. [ Resuit]The nematode specimens fixed by glutaraldehyde and osmium tetroxide and prepared by critical point drying were unmodified and unshrinkable with clear mor- phological structure, which could be scanned very well by scanning electron microscope. [ Conclusion ] The study provided reference for study and control of H. digonicus.展开更多
Chalkiness characters affect not only the grain appearance,milling,eating and cooking qualities but also the grain nutritional quality in rice,thus it is one of the most important traits in rice. It is very important ...Chalkiness characters affect not only the grain appearance,milling,eating and cooking qualities but also the grain nutritional quality in rice,thus it is one of the most important traits in rice. It is very important for us to investigate the relation of the chalkiness formation and the development of endosperm structure and starch granule of different rice varieties. Here,we have investigated the chalkiness characters such as chalkiness rate,chalkiness degree and chalkiness area in 15 japonica rice varieties from southern Henan. Furthermore,the endosperm structure and starch granules of rice grain were also observed with scanning electron microscope. The results showed that the 15 japonica varieties have a significantly linear relationship between the chalkiness rate and chalkiness degree. Among the varieties,the biggest difference is the chalkiness rate,the second is the chalkiness area,and the last is the chalkiness degree. Moreover,there is a certain correlation between the distribution of starch granules,the arrangement of endosperm cells and the occurrence of grain chalkiness in the different rice varieties. For the same variety,the starch granules of chalky and non-chalky grains have obvious difference,while the starch granules from the transparent part of chalky rice and non-chalky rice do not have significant difference. The results would provide useful references for the improvement of grain quality in rice.展开更多
In this work,an old scanning electron microscope(SEM)is refurbished to enhance its image processing capability.How to digitally sample and process an analog image is also presented.An NI PCI-6259 multiple input/output...In this work,an old scanning electron microscope(SEM)is refurbished to enhance its image processing capability.How to digitally sample and process an analog image is also presented.An NI PCI-6259 multiple input/output data acquisition(DAQ)board is used to acquire signals originally being sent to an analog display,and then convert the signals into a digital image.Two output channels are used for raster scan of the horizontal and verticle axes of the image buffer,while one input channel is used to read the brightness signals at various coordinate points.Synchronous method is used to maximize the DAQ speed.Finally,the digitally buffered images are read out to display and saved in a hard drive.The hardware and software designs of this work are explained in great detail,which can serve as a very good example for fast synchronous DAQ,advanced virtual instrument design and structural driver programming with LabVIEW.展开更多
This paper reports the ultrastructural changes of trichomonas vaginaiis (T. vag. ) under the action of s-(-)usnic acid sodium in vitro. These changes can be shown by the following results:At first, the degranulation o...This paper reports the ultrastructural changes of trichomonas vaginaiis (T. vag. ) under the action of s-(-)usnic acid sodium in vitro. These changes can be shown by the following results:At first, the degranulation of the rough endoplasmic reticulum (RER) took place, the polyribosomes disaggregated. And then, the Golgi complex and the endoplasmic reticulum dilated. The cytoplasmic matrix presented and inhomogeneous apperance. Finally, the biomembrane loosed and fractured.The cell nuclei presented the karyorrhexis.展开更多
SnS,a well-known van der Waals chalcogenide,is susceptible to oxidation in high-temperature or highhumidity environments,significantly impacting its functional performance and device stability.Conversely,oxidation can...SnS,a well-known van der Waals chalcogenide,is susceptible to oxidation in high-temperature or highhumidity environments,significantly impacting its functional performance and device stability.Conversely,oxidation can be used as an effective strategy for surface engineering,allowing for structure modulation or design,property tuning and application exploration.However,there is currently a gap in understanding the relationship between the oxidation behavior of SnS,the structure of its oxidized surface,and the dependence on oxidation temperature.In this study,we systematically investigated the evolution of SnS surfaces under thermal oxidation using electron microscopy.The microstructure evolution(e.g.,surface structures,phases,defects,and interface)of SnS during high-temperature oxidation has been fully characterized and studied based on cross-sectional samples.Various surface heterostructures were constructed,including SnO_(2)/SnS,SnO_(2)/SnS_(2)/SnS,and SnO_(2)/Sn_(2)S_(3)/SnS,offering significant potential for the surface functionalization of SnS-based systems.Accordingly,oxidation mechanisms at different stages were elucidated based on the detailed and clear picture of microstructures.This research not only deepens our understanding of the fundamental science of SnS oxidation but also provides valuable insights for preventing and developing surface oxidation engineering in SnS and other van der Waals chalcogenides/materials.展开更多
Electron microscope investigation on the red sea bream ( Pagrosomus major ), bastard halibut ( Paralichthys olivaceus ) and stone flounder ( Kareius bicoloratus ) in North China revealed virus infection in the bodies ...Electron microscope investigation on the red sea bream ( Pagrosomus major ), bastard halibut ( Paralichthys olivaceus ) and stone flounder ( Kareius bicoloratus ) in North China revealed virus infection in the bodies of the dead and diseased fish. These viruses included the lymphocystis disease virus (LDV), parvovirus, globular virus, and a kind of baculavirus which was not discovered and reported before and is now tentatively named baculavirus of stone flounder ( Kareius bicoloratus ).展开更多
Lithium-rich layered oxides(LrLOs) deliver extremely high specific capacities and are considered to be promising candidates for electric vehicle and smart grid applications. However, the application of LrLOs needs fur...Lithium-rich layered oxides(LrLOs) deliver extremely high specific capacities and are considered to be promising candidates for electric vehicle and smart grid applications. However, the application of LrLOs needs further understanding of the structural complexity and dynamic evolution of monoclinic and rhombohedral phases, in order to overcome the issues including voltage decay, poor rate capability, initial irreversible capacity loss and etc. The development of aberration correction for the transmission electron microscope and concurrent progress in electron spectroscopy, have fueled rapid progress in the understanding of the mechanism of such issues. New techniques based on the transmission electron microscope are first surveyed, and the applications of these techniques for the study of the structure, migration of transition metal, and the activation of oxygen of LrLOs are then explored in detail, with a particular focus on the mechanism of voltage decay.展开更多
Specimens of Mugil soiuy were collected from the coastal waters of Bohai Sea in Tianjin. The gillfilaments were cut with fine scissors and soaked for 24h in a fixative consisting of 2.5% glutaralde-hyde in 0.1 mol lph...Specimens of Mugil soiuy were collected from the coastal waters of Bohai Sea in Tianjin. The gillfilaments were cut with fine scissors and soaked for 24h in a fixative consisting of 2.5% glutaralde-hyde in 0.1 mol lphosporic acid buffer. After three washes in a buffer solution the tissue was fixedin a mixture of 1% osmium tetraoxide at 4℃ for 1h. The tissue was dehydrated in graded ethandethanols and dried. The tissue was examined and photographed with an SEM at an accelerating voltage展开更多
It is well known that β-BBO is a new type nonlinear optical crystal discovered in our institute and now β-BBO is widely used in the fields of laser and nonlinear optics. However, there are still some inclusions in ...It is well known that β-BBO is a new type nonlinear optical crystal discovered in our institute and now β-BBO is widely used in the fields of laser and nonlinear optics. However, there are still some inclusions in β-BBO crystal grown by flux method. Using AEM technique, we have studied the shape, composition and structure of inclusions which is very helpful to the explanation of the formation of inclusions.展开更多
Object To detect the healing between artificial bladder and tissue. Method:Healing between 4 artificial bladder and surrounding tissue was observed by scanning electronic microscope. Result 4 prosthesis all could heal...Object To detect the healing between artificial bladder and tissue. Method:Healing between 4 artificial bladder and surrounding tissue was observed by scanning electronic microscope. Result 4 prosthesis all could heal with tissue,but healing extent was different during different time. Conclusion:This artificial bladder could perform a good connection with tissue and the structure of nylon, surface area of prosthesis, time and infection maybe influenced the healing.展开更多
The construction of advanced laboratories for precision instruments, such as electron microscopes, involves unique challenges that are influenced by the specific environmental conditions required for optimal functiona...The construction of advanced laboratories for precision instruments, such as electron microscopes, involves unique challenges that are influenced by the specific environmental conditions required for optimal functionality. These include mitigating interference from magnetic fields and vibrations, which are critical for maintaining the precision and accuracy of the instruments used. This study aims to offer enhanced project management strategies and detailed construction solutions that address the environmental and technical needs specific to electron microscopy labs, thereby facilitating effective lab operations and extending the lifecycle of high-end precision instruments. Case studies of existing laboratory constructions, onsite investigations, and comprehensive reviews of the technical and environmental requirements provide the basis for a best practice for constructing sophisticated electron microscopy labs. The approach integrates both pre-construction planning and post-construction adjustments to create optimal operational environments. The findings suggest that successful lab constructions are those that incorporate thorough onsite assessments, strategic location choices, and the use of advanced construction materials and techniques specifically designed to counteract environmental challenges like magnetic and vibration interferences. Actionable guidelines for both planning and executing the construction of electron microscope labs highlighted in this tutorial are intended as an important resource to troubleshoot or upgrade existing lab facilities and to consult in preparation of future lab construction projects.展开更多
BACKGROUND: Primary intrahepatic cholelithiasis is usually combined with biliary tract infection. This research was undertaken to investigate the relationship between intrahepatic stones and biliary tract infection. M...BACKGROUND: Primary intrahepatic cholelithiasis is usually combined with biliary tract infection. This research was undertaken to investigate the relationship between intrahepatic stones and biliary tract infection. METHODS: Thirty-five bile samples and 30 stones specimens were cultured for bacteria and 12 stones specimens were examined with a scan electron microscope (SEM) or a transmission electron microscope (TEM). RESULT: 94.2% bile samples and 96.7% stones specimens were positive in bacteria culture. Bacteria were found in stones under SEM and TEM. CONCLUSION: Bacteria in stones are associated with the infection of the biliary tract.展开更多
AIM： To evaluate the effect of propolis administration on the healing of colon anastomosis with light and transmission electron microscopes. METHODS： Forty-eight Wistar-AIbino female rats were divided into two group...AIM： To evaluate the effect of propolis administration on the healing of colon anastomosis with light and transmission electron microscopes. METHODS： Forty-eight Wistar-AIbino female rats were divided into two groups and had colon resection and anastomosis. In group Ⅰ, rats were fed with standard rat chow pre- and postoperatively. The rats in group Ⅱ were fed with standard rat chow and began receiving oral supplementation of propolis 100 mg/kg per day beginning 7 d before the operation and continued until they were sacrificed. Rats were sacrificed 1, 3, 7 and 14 d after operation, and anastomotic bursting pressures measured. After the resection of anastomotic segments, histopathological examination was performed with light and transmission electron microscopes by two blinded histologists and photographed. RESULTS： The colonic bursting pressures of the propolis group were statistically significantly better than the control group. UItrastructural histopathological analysis of the colon anastomosis revealed that propotis accelerated the phases of the healing process and stimulated mature granulation tissue formation and collagen synthesis of fibroblasts. CONCLUSION： Bursting pressure measurements and ultra structural histopathological evaluation showed that administration of propolis accelerated the healing of colon anastomosis following surgical excision.展开更多
In-situ observation in high voltage electron microscope(HVEM) was used to investigate transformation from amorphous La(OH)3 to nanophase La2O3. Electron diffraction pattern indicates that the transition temperature fr...In-situ observation in high voltage electron microscope(HVEM) was used to investigate transformation from amorphous La(OH)3 to nanophase La2O3. Electron diffraction pattern indicates that the transition temperature from La(OH)3 to nanophase La2O3 is 400 ℃. Particle sizes for nanophase La2O3 are from 20 to 50 nm.展开更多
Three types of osteoblast-like cells with different cnfigurations could be ob-tained through culturing fetal chicken calvaria in vitro. They were spindle-shaped cells,globular cells, and polygonal or squamous cells. W...Three types of osteoblast-like cells with different cnfigurations could be ob-tained through culturing fetal chicken calvaria in vitro. They were spindle-shaped cells,globular cells, and polygonal or squamous cells. With passage of culture time, there werechanges in configuration so that the spindle-shaped cells and the globular cells turnedgradually into squamous cells, in quantity which increased greatly to produce confluenceand multi-layer formation of cells, and in function as evidenced by emergence ofintracytoplasmic granules, reflecting collagen synthesis.展开更多
A diplomonad flagellate Hexamita capsularis sp.nov.from the posterior intestine of the fish(Xenocypris dividi)from Liangzi Lake,Hubei Province,is described.The flagellate was oval,withsmooth surface and homogenous cyt...A diplomonad flagellate Hexamita capsularis sp.nov.from the posterior intestine of the fish(Xenocypris dividi)from Liangzi Lake,Hubei Province,is described.The flagellate was oval,withsmooth surface and homogenous cytoplasm,6.21±0.28μm in length,3.43±0.48μm in width and with anelliptical capsule.Two recurrent flagellae arranged between two nuclei ran longitudinally to the posteriorend.Fibrillar bands connected the accessory organelles of two cytostomal tubes,the body depressed atthe posterior end of the body.展开更多
文摘[Objective] The research aimed to provide reference for classification of pomegranate cultivars and studies of genetic relationship among pomegranate cultivars.[Method] The electron microscope scanning was used to comparatively observe leaf epidermal structures of 4 pomegranate cultivars.[Result] The upper epidermal structures of 4 pomegranate cultivars were similar and showed reticular structure .However, the differences existed in lower epidermis,such as cell shape,cell size and arrangement mode of cell as well as stomatal density,while the structures of leaf vein in lower epidermis of 4 pomegranate cultivars were similar.[Conclusion] The research provided morphological references for studying heterosis of pomegranate to some extent.
文摘Changes of calmodulin (CaM) distribution in the embryo sac of rice (Oryza sativa subsp. Japonica) at various stages before and after fertilization have been investigated by using immunogold electron microscopy. Before pollination, both cytoplasm and vacuoles of the egg cell, synergids and central cell were labeled by gold particles. A small amount of gold particles were localized in the nucleus, endoplasmic reticulum, mitochondria and dictyosomes. From pollination to fertilization, CaM amount increased in these cells, especially rich in the starch of amyloplasts. Increase of gold particles in the central cell began about 2 h earlier than that in the egg cell. There was no distinct difference of CaM amount between the degenerated and the persistent synergids. It is interesting to observe an obvious change of CaM distribution form during pollination and fertilization from scattered single particles to clustered particles, and back again to single particles after the fertilization finished. CaM was also localized extracellularly in the embryo sac wall as well as in the wall and intercellular space of nucellus cells. The extracellular CaM also changes in its amount and form after pollination. These results suggest that CaM, either intra- or extra-cellular, may play important roles in fertilization and zygote formation.
文摘[Objective]The experiment aimed to explore a new way for observing surface structure of Nostoc sphaeroides Kutzing. [Method] The scanning electron microscope was used to observe the epidermal ultrastructure of wild and cultured Nostoc sphaeroides Kutzing. [ Result] The epidermis of wild and cultured Nostoc sphaeroides Kutzing showed mixture structure of fibril colloid which was reticular arranged. The difference between wild and cultured Nostoc sphaeroides Kutzing was that the outer epidermis of cultured Nostoc sphaeroides Kutzing had trichome distribution but the wild Nostoc sphaeroides Kutzing did not has such distribution. The obsevation results of under smaller than 10 μm by scanning electron microscope was touched thick and showed many folds and distortions. [ Conclusion] The scanning electron microscope was an effective way to study development of Nostoc sphaeroides Kutzing colony and it was worth popularizing.
文摘[ Objective] The paper was to observe Helicotylenchus digonicus by scanning electron microscope （SEM）. [ Method ] H. digonicus collected from Changxing of Zhejiang Province was observed under scanning electron microscope, and its morphological structure was confmned under optical microscope. [ Resuit]The nematode specimens fixed by glutaraldehyde and osmium tetroxide and prepared by critical point drying were unmodified and unshrinkable with clear mor- phological structure, which could be scanned very well by scanning electron microscope. [ Conclusion ] The study provided reference for study and control of H. digonicus.
基金Supported by Key Project of Science and Technology in Henan Province(152102110100,152102110036)National Natural Science Foundation of China(U1604110,U1404319,31270727,31600992)+3 种基金Nanhu Scholars Program for Young Scholars of XYNU(2016056)Major Science and Technology Project in Henan Province(121100110200)Students Scientific Research Fund of Xinyang Normal University(2015-DXS-158)Fund of Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains(2016020)
文摘Chalkiness characters affect not only the grain appearance,milling,eating and cooking qualities but also the grain nutritional quality in rice,thus it is one of the most important traits in rice. It is very important for us to investigate the relation of the chalkiness formation and the development of endosperm structure and starch granule of different rice varieties. Here,we have investigated the chalkiness characters such as chalkiness rate,chalkiness degree and chalkiness area in 15 japonica rice varieties from southern Henan. Furthermore,the endosperm structure and starch granules of rice grain were also observed with scanning electron microscope. The results showed that the 15 japonica varieties have a significantly linear relationship between the chalkiness rate and chalkiness degree. Among the varieties,the biggest difference is the chalkiness rate,the second is the chalkiness area,and the last is the chalkiness degree. Moreover,there is a certain correlation between the distribution of starch granules,the arrangement of endosperm cells and the occurrence of grain chalkiness in the different rice varieties. For the same variety,the starch granules of chalky and non-chalky grains have obvious difference,while the starch granules from the transparent part of chalky rice and non-chalky rice do not have significant difference. The results would provide useful references for the improvement of grain quality in rice.
文摘In this work,an old scanning electron microscope(SEM)is refurbished to enhance its image processing capability.How to digitally sample and process an analog image is also presented.An NI PCI-6259 multiple input/output data acquisition(DAQ)board is used to acquire signals originally being sent to an analog display,and then convert the signals into a digital image.Two output channels are used for raster scan of the horizontal and verticle axes of the image buffer,while one input channel is used to read the brightness signals at various coordinate points.Synchronous method is used to maximize the DAQ speed.Finally,the digitally buffered images are read out to display and saved in a hard drive.The hardware and software designs of this work are explained in great detail,which can serve as a very good example for fast synchronous DAQ,advanced virtual instrument design and structural driver programming with LabVIEW.
文摘This paper reports the ultrastructural changes of trichomonas vaginaiis (T. vag. ) under the action of s-(-)usnic acid sodium in vitro. These changes can be shown by the following results:At first, the degranulation of the rough endoplasmic reticulum (RER) took place, the polyribosomes disaggregated. And then, the Golgi complex and the endoplasmic reticulum dilated. The cytoplasmic matrix presented and inhomogeneous apperance. Finally, the biomembrane loosed and fractured.The cell nuclei presented the karyorrhexis.
基金financially supported by the National Natural Science Foundation of China(Nos.11904039,52125103,52071041,U21A2054 and 12104071)。
文摘SnS,a well-known van der Waals chalcogenide,is susceptible to oxidation in high-temperature or highhumidity environments,significantly impacting its functional performance and device stability.Conversely,oxidation can be used as an effective strategy for surface engineering,allowing for structure modulation or design,property tuning and application exploration.However,there is currently a gap in understanding the relationship between the oxidation behavior of SnS,the structure of its oxidized surface,and the dependence on oxidation temperature.In this study,we systematically investigated the evolution of SnS surfaces under thermal oxidation using electron microscopy.The microstructure evolution(e.g.,surface structures,phases,defects,and interface)of SnS during high-temperature oxidation has been fully characterized and studied based on cross-sectional samples.Various surface heterostructures were constructed,including SnO_(2)/SnS,SnO_(2)/SnS_(2)/SnS,and SnO_(2)/Sn_(2)S_(3)/SnS,offering significant potential for the surface functionalization of SnS-based systems.Accordingly,oxidation mechanisms at different stages were elucidated based on the detailed and clear picture of microstructures.This research not only deepens our understanding of the fundamental science of SnS oxidation but also provides valuable insights for preventing and developing surface oxidation engineering in SnS and other van der Waals chalcogenides/materials.
文摘Electron microscope investigation on the red sea bream ( Pagrosomus major ), bastard halibut ( Paralichthys olivaceus ) and stone flounder ( Kareius bicoloratus ) in North China revealed virus infection in the bodies of the dead and diseased fish. These viruses included the lymphocystis disease virus (LDV), parvovirus, globular virus, and a kind of baculavirus which was not discovered and reported before and is now tentatively named baculavirus of stone flounder ( Kareius bicoloratus ).
基金finically supported by the National Key Research and Development Program of China (Grant No. 2016YFB0100100)Strategic Priority Research Program of Chinese Academy of Sciences (CAS, Grant No. XDA09010101)Ningbo Key Science and Technology Projects "Industrial Application Development of Graphene" (Grant No. 2014S10008)
文摘Lithium-rich layered oxides(LrLOs) deliver extremely high specific capacities and are considered to be promising candidates for electric vehicle and smart grid applications. However, the application of LrLOs needs further understanding of the structural complexity and dynamic evolution of monoclinic and rhombohedral phases, in order to overcome the issues including voltage decay, poor rate capability, initial irreversible capacity loss and etc. The development of aberration correction for the transmission electron microscope and concurrent progress in electron spectroscopy, have fueled rapid progress in the understanding of the mechanism of such issues. New techniques based on the transmission electron microscope are first surveyed, and the applications of these techniques for the study of the structure, migration of transition metal, and the activation of oxygen of LrLOs are then explored in detail, with a particular focus on the mechanism of voltage decay.
文摘Specimens of Mugil soiuy were collected from the coastal waters of Bohai Sea in Tianjin. The gillfilaments were cut with fine scissors and soaked for 24h in a fixative consisting of 2.5% glutaralde-hyde in 0.1 mol lphosporic acid buffer. After three washes in a buffer solution the tissue was fixedin a mixture of 1% osmium tetraoxide at 4℃ for 1h. The tissue was dehydrated in graded ethandethanols and dried. The tissue was examined and photographed with an SEM at an accelerating voltage
文摘It is well known that β-BBO is a new type nonlinear optical crystal discovered in our institute and now β-BBO is widely used in the fields of laser and nonlinear optics. However, there are still some inclusions in β-BBO crystal grown by flux method. Using AEM technique, we have studied the shape, composition and structure of inclusions which is very helpful to the explanation of the formation of inclusions.
文摘Object To detect the healing between artificial bladder and tissue. Method:Healing between 4 artificial bladder and surrounding tissue was observed by scanning electronic microscope. Result 4 prosthesis all could heal with tissue,but healing extent was different during different time. Conclusion:This artificial bladder could perform a good connection with tissue and the structure of nylon, surface area of prosthesis, time and infection maybe influenced the healing.
文摘The construction of advanced laboratories for precision instruments, such as electron microscopes, involves unique challenges that are influenced by the specific environmental conditions required for optimal functionality. These include mitigating interference from magnetic fields and vibrations, which are critical for maintaining the precision and accuracy of the instruments used. This study aims to offer enhanced project management strategies and detailed construction solutions that address the environmental and technical needs specific to electron microscopy labs, thereby facilitating effective lab operations and extending the lifecycle of high-end precision instruments. Case studies of existing laboratory constructions, onsite investigations, and comprehensive reviews of the technical and environmental requirements provide the basis for a best practice for constructing sophisticated electron microscopy labs. The approach integrates both pre-construction planning and post-construction adjustments to create optimal operational environments. The findings suggest that successful lab constructions are those that incorporate thorough onsite assessments, strategic location choices, and the use of advanced construction materials and techniques specifically designed to counteract environmental challenges like magnetic and vibration interferences. Actionable guidelines for both planning and executing the construction of electron microscope labs highlighted in this tutorial are intended as an important resource to troubleshoot or upgrade existing lab facilities and to consult in preparation of future lab construction projects.
文摘BACKGROUND: Primary intrahepatic cholelithiasis is usually combined with biliary tract infection. This research was undertaken to investigate the relationship between intrahepatic stones and biliary tract infection. METHODS: Thirty-five bile samples and 30 stones specimens were cultured for bacteria and 12 stones specimens were examined with a scan electron microscope (SEM) or a transmission electron microscope (TEM). RESULT: 94.2% bile samples and 96.7% stones specimens were positive in bacteria culture. Bacteria were found in stones under SEM and TEM. CONCLUSION: Bacteria in stones are associated with the infection of the biliary tract.
文摘AIM： To evaluate the effect of propolis administration on the healing of colon anastomosis with light and transmission electron microscopes. METHODS： Forty-eight Wistar-AIbino female rats were divided into two groups and had colon resection and anastomosis. In group Ⅰ, rats were fed with standard rat chow pre- and postoperatively. The rats in group Ⅱ were fed with standard rat chow and began receiving oral supplementation of propolis 100 mg/kg per day beginning 7 d before the operation and continued until they were sacrificed. Rats were sacrificed 1, 3, 7 and 14 d after operation, and anastomotic bursting pressures measured. After the resection of anastomotic segments, histopathological examination was performed with light and transmission electron microscopes by two blinded histologists and photographed. RESULTS： The colonic bursting pressures of the propolis group were statistically significantly better than the control group. UItrastructural histopathological analysis of the colon anastomosis revealed that propotis accelerated the phases of the healing process and stimulated mature granulation tissue formation and collagen synthesis of fibroblasts. CONCLUSION： Bursting pressure measurements and ultra structural histopathological evaluation showed that administration of propolis accelerated the healing of colon anastomosis following surgical excision.
文摘In-situ observation in high voltage electron microscope(HVEM) was used to investigate transformation from amorphous La(OH)3 to nanophase La2O3. Electron diffraction pattern indicates that the transition temperature from La(OH)3 to nanophase La2O3 is 400 ℃. Particle sizes for nanophase La2O3 are from 20 to 50 nm.
文摘Three types of osteoblast-like cells with different cnfigurations could be ob-tained through culturing fetal chicken calvaria in vitro. They were spindle-shaped cells,globular cells, and polygonal or squamous cells. With passage of culture time, there werechanges in configuration so that the spindle-shaped cells and the globular cells turnedgradually into squamous cells, in quantity which increased greatly to produce confluenceand multi-layer formation of cells, and in function as evidenced by emergence ofintracytoplasmic granules, reflecting collagen synthesis.
基金This work is a part of the M.S.thesis of Xiao Wu-han and was supported by the National Natural Science Foundation of China (Grant NO.3870489).
文摘A diplomonad flagellate Hexamita capsularis sp.nov.from the posterior intestine of the fish(Xenocypris dividi)from Liangzi Lake,Hubei Province,is described.The flagellate was oval,withsmooth surface and homogenous cytoplasm,6.21±0.28μm in length,3.43±0.48μm in width and with anelliptical capsule.Two recurrent flagellae arranged between two nuclei ran longitudinally to the posteriorend.Fibrillar bands connected the accessory organelles of two cytostomal tubes,the body depressed atthe posterior end of the body.
