Purpose-Rail corrugation is still one of the unsolved challenges in the railway industry,and the abnormal vibration and high-frequency noise caused by it constitute serious adverse effects on the operating environment...Purpose-Rail corrugation is still one of the unsolved challenges in the railway industry,and the abnormal vibration and high-frequency noise caused by it constitute serious adverse effects on the operating environment.How to control corrugation has been an important research theme,and understanding corrugation evolution features is the necessary prerequisite.This study aims to investigate the specific evolution characteristics of corrugation from the contact stick-slip perspective.Design/methodology/approach-The formation and development processes of corrugation are analyzed by using a self-designed scale-down test device.Specifically,the contact stick-slip characteristics under different creepage conditions are analyzed and the formation mechanism of corrugation is summarized.On the basis of corrugation formation,the trend of corrugation development is further emphasized to completely describe the whole process of corrugation evolution.Findings-The results show that,under the determined vertical load condition,the contact interface appears the creep force-creepage negative slope phenomenon in the transverse direction.The cause of short-pitch corrugation on the rail wheel surface under the smaller angles of attack may be related to the inherent vibration frequency of the test device,and the cause of corrugation on the rail wheel surface under the larger angles of attack is mainly related to the stick-slip vibration induced by contact creep saturation.Originality/value-This research explores the evolution characteristics of corrugation by adopting a selfdesigned scale-down test device,and elucidates the mechanism of corrugation in detail.展开更多
Bioprocess scale-down simulators are used to investigate the phenomena occurring in industrial scale cultivations. The aim is to simulate the large-scale conditions, characterized by long mixing times introducing loca...Bioprocess scale-down simulators are used to investigate the phenomena occurring in industrial scale cultivations. The aim is to simulate the large-scale conditions, characterized by long mixing times introducing local gradients into the bioreactor, in laboratory scale bioreactor. We have expanded the current physiological knowledge about the consequences of such cultivation conditions from well-known mixed-acid fermentation to amino acid metabolism. Several interesting deviations were observed for amino acids derived from pyruvate when a STR-PFR scale-down simulator and a regular STR fed-batch cultivation were compared. In this article we show that the cultivation of Escherichia coli K-12 substrain W3110 in a scale-down two-compartment bioreactor has consequences on the free amino acid pools. Repeated occurrence of high glucose zones affects the biosynthesis of amino acids derived from pyruvate. This is interesting since the pyruvate based amino acids are precursors of the branched chain amino acid pathway, which is further disturbed by an unbalanced regulation caused by a frameshift mutation in the ilvG gene of E. coli K-12 strains. As a consequence the formation of non-typical amino acid norvaline was detected in the two compartment bioreactor cultivation.展开更多
基金funded by the Science and Technology Research Project of Universities in Hebei Province(No.QN2025314)Youth Specialization Fund for State Key Laboratory(No.50110010766)Shanghai Key Laboratory of Rail Infrastructure Durability and System Safety(No.R202405).
文摘Purpose-Rail corrugation is still one of the unsolved challenges in the railway industry,and the abnormal vibration and high-frequency noise caused by it constitute serious adverse effects on the operating environment.How to control corrugation has been an important research theme,and understanding corrugation evolution features is the necessary prerequisite.This study aims to investigate the specific evolution characteristics of corrugation from the contact stick-slip perspective.Design/methodology/approach-The formation and development processes of corrugation are analyzed by using a self-designed scale-down test device.Specifically,the contact stick-slip characteristics under different creepage conditions are analyzed and the formation mechanism of corrugation is summarized.On the basis of corrugation formation,the trend of corrugation development is further emphasized to completely describe the whole process of corrugation evolution.Findings-The results show that,under the determined vertical load condition,the contact interface appears the creep force-creepage negative slope phenomenon in the transverse direction.The cause of short-pitch corrugation on the rail wheel surface under the smaller angles of attack may be related to the inherent vibration frequency of the test device,and the cause of corrugation on the rail wheel surface under the larger angles of attack is mainly related to the stick-slip vibration induced by contact creep saturation.Originality/value-This research explores the evolution characteristics of corrugation by adopting a selfdesigned scale-down test device,and elucidates the mechanism of corrugation in detail.
文摘Bioprocess scale-down simulators are used to investigate the phenomena occurring in industrial scale cultivations. The aim is to simulate the large-scale conditions, characterized by long mixing times introducing local gradients into the bioreactor, in laboratory scale bioreactor. We have expanded the current physiological knowledge about the consequences of such cultivation conditions from well-known mixed-acid fermentation to amino acid metabolism. Several interesting deviations were observed for amino acids derived from pyruvate when a STR-PFR scale-down simulator and a regular STR fed-batch cultivation were compared. In this article we show that the cultivation of Escherichia coli K-12 substrain W3110 in a scale-down two-compartment bioreactor has consequences on the free amino acid pools. Repeated occurrence of high glucose zones affects the biosynthesis of amino acids derived from pyruvate. This is interesting since the pyruvate based amino acids are precursors of the branched chain amino acid pathway, which is further disturbed by an unbalanced regulation caused by a frameshift mutation in the ilvG gene of E. coli K-12 strains. As a consequence the formation of non-typical amino acid norvaline was detected in the two compartment bioreactor cultivation.
