Taizhou Bridge is the first kilometer level three-pylon two-span suspension bridge in the world and the structural complexity has significant effects on the seismic performance of the bridge. Shaking table test of Tai...Taizhou Bridge is the first kilometer level three-pylon two-span suspension bridge in the world and the structural complexity has significant effects on the seismic performance of the bridge. Shaking table test of Taizhou Bridge is arranged to investigate the effects of non-uniform ground motion input, collision between main and side spans and optimal seismic structural system. It's very important and difficult to design and manufacture the scaled down model of Taizhou Bridge used during the shaking table test. The key point is that the girder and pylons are very hard to be manufactured if the similarity ratio is strictly followed. Based on the finite element method (FEM) analysis, a simplified scaled down model is designed and the bending stiffness of the girder and pylon are strictly simulated, and the torsion stiffness and axial stiffness are not strictly simulated. The inner forces and displacements of critical sections, points of simplified model and theoretical model are compared by FEM analysis, and it's found out that the difference between the seismic responses is relatively small. So, the simplified model can be used to conduct the shaking table test by the FEM verification.展开更多
A down-scaled operational oceanographic system is developed for the coastal waters of Korea using a re- gional ocean modeling system （ROMS）. The operational oceanographic modeling system consists of at- mospheric an...A down-scaled operational oceanographic system is developed for the coastal waters of Korea using a re- gional ocean modeling system （ROMS）. The operational oceanographic modeling system consists of at- mospheric and hydrodynamic models. The hydrodynamic model, ROMS, is coupled with wave, sediment transport, and water quality modules. The system forecasts the predicted results twice a day on a 72 h basis, including sea surface elevation, currents, temperature, salinity, storm surge height, and wave information for the coastal waters of Korea. The predicted results are exported to the web-GIS-based coastal informa- tion system for real-time dissemination to the public and validation with real-time monitoring data using visualization technologies. The ROMS is two-way coupled with a simulating waves nearshore model, SWAN, for the hydrodynamics and waves, nested with the meteorological model, WRE for the atmospheric surface forcing, and externally nested with the eutrophication model, CE-QUAL-ICM, for the water quality. The op- erational model, ROMS, was calibrated with the tidal surface observed with a tide-gage and verified with current data observed by bottom-mounted ADCP or AWAC near the coastal waters of Korea. To validate the predicted results, we used real-time monitoring data derived from remote buoy system, HF-radar, and geostationary ocean color imager （GOCI）. This down-scaled operational coastal forecasting system will be used as a part of the Korea operational oceanographic system （KOOS） with other operational oceanographic systems.展开更多
目的通过构建15 L缩小模型验证重组人干扰素(recombinant human interferon,rhIFN)α2b工作代菌种在关键代次的传代稳定性,以确保rhIFNα2b在生产过程中的稳定性和有效性。方法将rhIFNα2b冻干菌种复苏并进行传代培养,分别对第0、10、20...目的通过构建15 L缩小模型验证重组人干扰素(recombinant human interferon,rhIFN)α2b工作代菌种在关键代次的传代稳定性,以确保rhIFNα2b在生产过程中的稳定性和有效性。方法将rhIFNα2b冻干菌种复苏并进行传代培养,分别对第0、10、20、30、40、50代菌液保存甘油菌。选取传代稳定性研究中保存的各代次甘油菌样品,在模拟生产条件的15 L缩小模型发酵系统中进行发酵-表达试验,通过实时监测菌体生长曲线,结合终点检测指标(产物表达量、生物学活性、质粒稳定性、遗传完整性以及微生物纯度等)进行全面比对,以判断限传代次是否可覆盖现有培养工艺。结果rhIFNα2b工作代菌种第0、10、20、30、40和50代甘油菌在连续传代培养后,表现出良好的稳定性和一致性。各代次菌种在LB平板上均呈现典型的大肠埃希菌菌落形态,无杂菌污染;抗生素抗性未发生改变;革兰染色结果均为革兰阴性;生化反应特性符合大肠埃希菌典型特征;目标基因表达量始终高于20%,且无下降趋势;发酵活性维持在1.0×10^(8)IU/L以上;质粒酶切图谱与理论图谱一致,序列未发生变化,质粒丢失率<30%,表现出较好的遗传稳定性。结论rhIFNα2b工作代菌种在缩小模型模拟生产规模发酵时发酵培养的稳定性良好,现有工艺条件下菌种的限传代次能够有效覆盖变更后的生产需求,为工艺优化提供了重要的数据支撑。展开更多
充分利用气温、降水量空间统计分析的结果,通过在模型中引入坡度(SL)、坡向(SD)变量,对祁连山中东部地区气温、降水量空间变化模式进行尺度下移,得到了该地区具有多尺度特性的高空间分辨率气温、降水量的分析模型:T=a+bH-KT cos SD sin ...充分利用气温、降水量空间统计分析的结果,通过在模型中引入坡度(SL)、坡向(SD)变量,对祁连山中东部地区气温、降水量空间变化模式进行尺度下移,得到了该地区具有多尺度特性的高空间分辨率气温、降水量的分析模型:T=a+bH-KT cos SD sin SL,P=(a+blnH+cln Y)(1+Kp sin SL); 进而计算了该地区7月份和年平均气温的分布式模型和年降水量分布式模型,得到了相应的栅格地图．验证结果表明这两个模型可以与山地冰川和森林分布区的气候分析资料很好地匹配．根据年降水量栅格地图的统计分析,将本区域内的最大降水高度带确定在4500 m．展开更多
基金National Science and Technology Support Program of China(No.2009BAG15B01)Key Programs for Science and Technology Development of Chinese Transportation Industry(No.2008-353-332-190)+2 种基金Natural Science Foundation of China(No.50708074)the Ministry of Science and Technology of China(No.SLDRCE08-B-04)Kwang-Hua Fund for College of Civil Engineering,Tongji University
文摘Taizhou Bridge is the first kilometer level three-pylon two-span suspension bridge in the world and the structural complexity has significant effects on the seismic performance of the bridge. Shaking table test of Taizhou Bridge is arranged to investigate the effects of non-uniform ground motion input, collision between main and side spans and optimal seismic structural system. It's very important and difficult to design and manufacture the scaled down model of Taizhou Bridge used during the shaking table test. The key point is that the girder and pylons are very hard to be manufactured if the similarity ratio is strictly followed. Based on the finite element method (FEM) analysis, a simplified scaled down model is designed and the bending stiffness of the girder and pylon are strictly simulated, and the torsion stiffness and axial stiffness are not strictly simulated. The inner forces and displacements of critical sections, points of simplified model and theoretical model are compared by FEM analysis, and it's found out that the difference between the seismic responses is relatively small. So, the simplified model can be used to conduct the shaking table test by the FEM verification.
基金The project entitled Cooperation on the Development of Basic Technologies for the Yellow Sea and East China Sea Operational Oceanographic System funded by the China-Korea Joint Ocean Research Centerthe project entitled"Development of Korea Operational Oceanographic System"funded by the Ministry of Oceans and Fisheries,Koreathe project Functional Improvement of Korea Ocean Satellite Center and Development of the Marine Environment Impact Prediction Program funded by the Korea Institute of Ocean Science and Technology
文摘A down-scaled operational oceanographic system is developed for the coastal waters of Korea using a re- gional ocean modeling system （ROMS）. The operational oceanographic modeling system consists of at- mospheric and hydrodynamic models. The hydrodynamic model, ROMS, is coupled with wave, sediment transport, and water quality modules. The system forecasts the predicted results twice a day on a 72 h basis, including sea surface elevation, currents, temperature, salinity, storm surge height, and wave information for the coastal waters of Korea. The predicted results are exported to the web-GIS-based coastal informa- tion system for real-time dissemination to the public and validation with real-time monitoring data using visualization technologies. The ROMS is two-way coupled with a simulating waves nearshore model, SWAN, for the hydrodynamics and waves, nested with the meteorological model, WRE for the atmospheric surface forcing, and externally nested with the eutrophication model, CE-QUAL-ICM, for the water quality. The op- erational model, ROMS, was calibrated with the tidal surface observed with a tide-gage and verified with current data observed by bottom-mounted ADCP or AWAC near the coastal waters of Korea. To validate the predicted results, we used real-time monitoring data derived from remote buoy system, HF-radar, and geostationary ocean color imager （GOCI）. This down-scaled operational coastal forecasting system will be used as a part of the Korea operational oceanographic system （KOOS） with other operational oceanographic systems.
文摘目的通过构建15 L缩小模型验证重组人干扰素(recombinant human interferon,rhIFN)α2b工作代菌种在关键代次的传代稳定性,以确保rhIFNα2b在生产过程中的稳定性和有效性。方法将rhIFNα2b冻干菌种复苏并进行传代培养,分别对第0、10、20、30、40、50代菌液保存甘油菌。选取传代稳定性研究中保存的各代次甘油菌样品,在模拟生产条件的15 L缩小模型发酵系统中进行发酵-表达试验,通过实时监测菌体生长曲线,结合终点检测指标(产物表达量、生物学活性、质粒稳定性、遗传完整性以及微生物纯度等)进行全面比对,以判断限传代次是否可覆盖现有培养工艺。结果rhIFNα2b工作代菌种第0、10、20、30、40和50代甘油菌在连续传代培养后,表现出良好的稳定性和一致性。各代次菌种在LB平板上均呈现典型的大肠埃希菌菌落形态,无杂菌污染;抗生素抗性未发生改变;革兰染色结果均为革兰阴性;生化反应特性符合大肠埃希菌典型特征;目标基因表达量始终高于20%,且无下降趋势;发酵活性维持在1.0×10^(8)IU/L以上;质粒酶切图谱与理论图谱一致,序列未发生变化,质粒丢失率<30%,表现出较好的遗传稳定性。结论rhIFNα2b工作代菌种在缩小模型模拟生产规模发酵时发酵培养的稳定性良好,现有工艺条件下菌种的限传代次能够有效覆盖变更后的生产需求,为工艺优化提供了重要的数据支撑。
文摘充分利用气温、降水量空间统计分析的结果,通过在模型中引入坡度(SL)、坡向(SD)变量,对祁连山中东部地区气温、降水量空间变化模式进行尺度下移,得到了该地区具有多尺度特性的高空间分辨率气温、降水量的分析模型:T=a+bH-KT cos SD sin SL,P=(a+blnH+cln Y)(1+Kp sin SL); 进而计算了该地区7月份和年平均气温的分布式模型和年降水量分布式模型,得到了相应的栅格地图．验证结果表明这两个模型可以与山地冰川和森林分布区的气候分析资料很好地匹配．根据年降水量栅格地图的统计分析,将本区域内的最大降水高度带确定在4500 m．
