Objective:To conduct a systemic evaluation of the medicinal value of seeds which include macroscopic and microscopic characterization,physiochomical evaluation,preliminary phylochemical screening and experimental anti...Objective:To conduct a systemic evaluation of the medicinal value of seeds which include macroscopic and microscopic characterization,physiochomical evaluation,preliminary phylochemical screening and experimental antipyretic activity.Methods:Saraca asoca seed was studied for pharmacognostical,phytochemical and other recommended methods for standardizations.Also,the acetone extract of the seeds was evaluated for acute toxicity study and antipyretic activity using Brewer's yeast induced pyrexia in Wislar rats at oral doses of 300 mg/kg and 500 mg/kg.Results:After phytochemical screening,the acetone extract showed the presence of saponin,tannins and flavonoids which inhibit pyrexia.The therapeutic efficacy achieved at both the dose levels of the research drug and standard drug aspirin(100 mg/kg)showed significant(P<0.01)antipyretic activity when compared to the control group.The highly significant antipyretic effect exhibited at the dose of 500 mg/kg was also found to be sustainable in nature.Conclusions:The antipyretic effect of the acetone extract showed significant results in rats at the dose of 500 mg/kg after following the standard pharmacognostical and phylochemieal met hods.展开更多
Forest trees in general and those belonging to family Fabaceae in particular, have proved to be recalcitrant for propagation through tissue culture. Saraca asoca (Roxb.) Willd. (Family-Caesalpinaceae) is one such tree...Forest trees in general and those belonging to family Fabaceae in particular, have proved to be recalcitrant for propagation through tissue culture. Saraca asoca (Roxb.) Willd. (Family-Caesalpinaceae) is one such tree which has become vulnerable in nature due to over exploitation of its bark. Four nutrient media [MS (Murashige and Skoog Medium), WPM (Woody Plant Medium), B5 (Gamborg’s Medium) and NN (Nitsch and Nitsch Medium, 1969)] and five doses of BA (N6-Benzyladenine) (0, 2.2, 4.4, 8.8 and 17.8 μM) and their all possible interactions were tested for shoot induction and proliferation from nodal segments of 3-year-old plants. B5 medium supplemented with 2.2 μM BA was screened out as the most suitable medium shoot induction, proliferation and elongation of regenerated shoots. In order to enhance shoot number, the nitrogen source in B5 medium was modified and five strengths of KNO3 (0.25×, 0.5×, 1.0×, 1.25× and 1.5×) were tested. The different strengths of KNO3 (Potassium nitrate) had statistically significant effect on number of shoots and on 0.25× strength of KNO3, maximum number of shoots (1.92) were obtained. The modified strengths of KNO3 did not significantly affect the elongation of shoots. Effect of 5 durations (quick dip, transfer of shoots after 1 day, after 3 days, after 5 days and after 7 days) of pulse treatment with 200 μM IBA (Indole-3-butyric acid) in 1/2 strength MS liquid medium was tested. Thereafter, the shoots were transferred to semi-solid half strength MS medium supplemented with 0.2 μM IBA and 3.96 μM phloroglucinol. Pulse treatment of 5 days duration resulted in 37.5% in vitro rooting of shoots. Plantlets were hardened in soilrite soaked with half strength MS medium in culture room and later shifted to a soil mixture in shade house.展开更多
The present study reports an unprecedented biogenic method for the synthesis of silver nanoparticles(AgNPs)using leaf extract of Saraca indica and characterized their antibacterial activity.We have also focused on the...The present study reports an unprecedented biogenic method for the synthesis of silver nanoparticles(AgNPs)using leaf extract of Saraca indica and characterized their antibacterial activity.We have also focused on the biosynthesis mechanism of AgNPs.Plant leaf extract has water soluble organic materials which help in the reduction of silver ions and stabilization of AgNPs.Aqueous solution of silver nitrate was treated with leaf extract of Saraca indica for the formation of AgNPs.The surface plasmon resonance was occurred at 412 nm.The size distribution profile of synthesized AgNPs was analysed by Dynamic Light Scattering(DLS).Transmission Electron Microscopy(TEM)has been done for the measurement of particle size and their morphology.The role of phytochemicals in the reduction of silver ions and defining the framework in which AgNPs are covered and provide steadiness can be determined through Fourier Transform Infrared Spectroscopy(FTIR).TEM micrograph reveals that the size of AgNPs was obtained in the range of 13-50 nm with spherical morphology.X-ray Diffraction(XRD)pattern of the AgNPs exhibited 2θvalues corresponding to the silver nanocrystals.Furthermore,the antibacterial activity of synthesized AgNPs against E.coli DH5αwas investigated by growth curve and inhibition zone analysis.It was observed that the 20µg/ml concentration of biogenic AgNPs recorded as minimal inhibitory concentration(MIC)against E.coli DH5α.展开更多
基金Supported by University Grants Commission.New Delhi[Grantsanctioned vide no-F.No.37-496/2009(SR)]
文摘Objective:To conduct a systemic evaluation of the medicinal value of seeds which include macroscopic and microscopic characterization,physiochomical evaluation,preliminary phylochemical screening and experimental antipyretic activity.Methods:Saraca asoca seed was studied for pharmacognostical,phytochemical and other recommended methods for standardizations.Also,the acetone extract of the seeds was evaluated for acute toxicity study and antipyretic activity using Brewer's yeast induced pyrexia in Wislar rats at oral doses of 300 mg/kg and 500 mg/kg.Results:After phytochemical screening,the acetone extract showed the presence of saponin,tannins and flavonoids which inhibit pyrexia.The therapeutic efficacy achieved at both the dose levels of the research drug and standard drug aspirin(100 mg/kg)showed significant(P<0.01)antipyretic activity when compared to the control group.The highly significant antipyretic effect exhibited at the dose of 500 mg/kg was also found to be sustainable in nature.Conclusions:The antipyretic effect of the acetone extract showed significant results in rats at the dose of 500 mg/kg after following the standard pharmacognostical and phylochemieal met hods.
文摘Forest trees in general and those belonging to family Fabaceae in particular, have proved to be recalcitrant for propagation through tissue culture. Saraca asoca (Roxb.) Willd. (Family-Caesalpinaceae) is one such tree which has become vulnerable in nature due to over exploitation of its bark. Four nutrient media [MS (Murashige and Skoog Medium), WPM (Woody Plant Medium), B5 (Gamborg’s Medium) and NN (Nitsch and Nitsch Medium, 1969)] and five doses of BA (N6-Benzyladenine) (0, 2.2, 4.4, 8.8 and 17.8 μM) and their all possible interactions were tested for shoot induction and proliferation from nodal segments of 3-year-old plants. B5 medium supplemented with 2.2 μM BA was screened out as the most suitable medium shoot induction, proliferation and elongation of regenerated shoots. In order to enhance shoot number, the nitrogen source in B5 medium was modified and five strengths of KNO3 (0.25×, 0.5×, 1.0×, 1.25× and 1.5×) were tested. The different strengths of KNO3 (Potassium nitrate) had statistically significant effect on number of shoots and on 0.25× strength of KNO3, maximum number of shoots (1.92) were obtained. The modified strengths of KNO3 did not significantly affect the elongation of shoots. Effect of 5 durations (quick dip, transfer of shoots after 1 day, after 3 days, after 5 days and after 7 days) of pulse treatment with 200 μM IBA (Indole-3-butyric acid) in 1/2 strength MS liquid medium was tested. Thereafter, the shoots were transferred to semi-solid half strength MS medium supplemented with 0.2 μM IBA and 3.96 μM phloroglucinol. Pulse treatment of 5 days duration resulted in 37.5% in vitro rooting of shoots. Plantlets were hardened in soilrite soaked with half strength MS medium in culture room and later shifted to a soil mixture in shade house.
文摘The present study reports an unprecedented biogenic method for the synthesis of silver nanoparticles(AgNPs)using leaf extract of Saraca indica and characterized their antibacterial activity.We have also focused on the biosynthesis mechanism of AgNPs.Plant leaf extract has water soluble organic materials which help in the reduction of silver ions and stabilization of AgNPs.Aqueous solution of silver nitrate was treated with leaf extract of Saraca indica for the formation of AgNPs.The surface plasmon resonance was occurred at 412 nm.The size distribution profile of synthesized AgNPs was analysed by Dynamic Light Scattering(DLS).Transmission Electron Microscopy(TEM)has been done for the measurement of particle size and their morphology.The role of phytochemicals in the reduction of silver ions and defining the framework in which AgNPs are covered and provide steadiness can be determined through Fourier Transform Infrared Spectroscopy(FTIR).TEM micrograph reveals that the size of AgNPs was obtained in the range of 13-50 nm with spherical morphology.X-ray Diffraction(XRD)pattern of the AgNPs exhibited 2θvalues corresponding to the silver nanocrystals.Furthermore,the antibacterial activity of synthesized AgNPs against E.coli DH5αwas investigated by growth curve and inhibition zone analysis.It was observed that the 20µg/ml concentration of biogenic AgNPs recorded as minimal inhibitory concentration(MIC)against E.coli DH5α.
