OBJECTIVE:To evaluate the therapeutic effects of Xiahuo Pingwei San(夏藿平胃散,XHPWS)on ulcerative colitis(UC)in mice and to explore the underlying mechanisms through a network pharmacology approach.METHODS:Ultra-perf...OBJECTIVE:To evaluate the therapeutic effects of Xiahuo Pingwei San(夏藿平胃散,XHPWS)on ulcerative colitis(UC)in mice and to explore the underlying mechanisms through a network pharmacology approach.METHODS:Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS)was utilized to identify the chemical composition and authenticate the active constituents of XHPWS,ensuring rigorous quality control across batches.A dextran sulfate sodium(DSS)-induced UC model was established in C57BL/6 mice,which were treated with XHPWS in vivo.The efficacy against UC was assessed by measuring parameters such as body weight,disease activity index(DAI)scores,and colon length.Levels of inflammatory cytokines,including interleukin-6(IL-6),interleukin-1β(IL-1β),and tumor necrosis factor-alpha(TNF-α),in colonic tissue were evaluated using enzymelinked immunosorbent assay(ELISA).Histological analysis of colon sections was conducted using hematoxylin and eosin staining.A network pharmacology approach was employed to explore the mechanisms of XHPWS and to predict its potential targets in UC treatment.Predicted protein expressions in colonic tissue were validated using immune-ohistochemistry(IHC)and Western blotting techniques.RESULTS:XHPWS effectively alle via ted DSS-induced UC symptoms in mice,as evidenced by restored body weight,reduced colon shortening,and decreased DAI scores.Histopathological examination revealed that XHPWS significantly reduced intestinal inflammatory infiltration,restored intestinal epithelial permeability,and increased goblet cell count.Network pharmacology analysis identified 63 active compounds in XHPWS and suggested that it might target 35 potential proteins associated with UC treatment.Functional enrichment analysis indicated that the protective mechanism of XHPWS could be related to the advanced glycation end products-receptor for advanced glycation end products(AGE-RAGE)signaling pathway.Notably,quercetin,kaempferol,wogonin,and nobiletin,the main components of XHPWS,showed strong correlations with the core targets.Additionally,experimental validation demonstrated that XHPWS significantly decreased levels of inflammatory cytokines interleukin 6(IL-6),interleukin 1 beta(IL-1β),and tumor necrosis factor alpha(TNF-α)in UC mice,while downregulating the expression of proteins related to the AGE-RAGE pathway.CONCLUSION:Our study demonstrated that XHPWS effectively alle via tes colitis symptoms and inflammation in UC mice,potentially through the regulation of the AGE-RAGE pathway.These findings provide strong evidence for the therapeutic potential of XHPWS in UC treatment,thereby broadening its clinical applications.展开更多
采用种子乳液聚合技术在聚丁二烯(PB)乳胶粒子上接枝共聚苯乙烯(St)和丙烯腈(AN)合成了一系列PB质量分数为40%的PB g SAN弹性体·研究了共聚单体中叔 十二烷基硫醇(TDDM)质量分数对SAN共聚物接枝行为、弹性体粒子结构及其动态力学...采用种子乳液聚合技术在聚丁二烯(PB)乳胶粒子上接枝共聚苯乙烯(St)和丙烯腈(AN)合成了一系列PB质量分数为40%的PB g SAN弹性体·研究了共聚单体中叔 十二烷基硫醇(TDDM)质量分数对SAN共聚物接枝行为、弹性体粒子结构及其动态力学性能的影响·结果发现,随着单体中TDDM质量分数的增加,SAN在PB上的接枝率和接枝效率降低,弹性体粒子中SAN包容结构的尺寸增大,在低温区的动态剪切模量下降·将其与SAN树脂共混后发现在共聚单体中引入TDDM后提高了弹性体粒子的增韧效率·展开更多
The study of the electrical structure and fluid content of the southern San Andreas Fault(SSAF)plays a significant role in understanding the geological processes and earthquake genesis.The paper analyzes the Bahr skew...The study of the electrical structure and fluid content of the southern San Andreas Fault(SSAF)plays a significant role in understanding the geological processes and earthquake genesis.The paper analyzes the Bahr skew and G-B decomposition from magnetotelluric sounding data in the SSAF to determine the strike direction is north-eastward 135°.Using the Nonlinear Conjugate Gradient algorithm,a reliable 2D electrical structure model was obtained.The Mission Creek and Banning faults,components of the SSAF,exhibit high conductivity within the crust,whereas the Eastern Transverse Ranges to the northeast of the fault show high resistivity characteristics.By integrating the modified Archie's law,the relations between conductivity,temperature,salinity,and fluid content were established,leading to conductivity-temperature and conductivity-fluid content relationships.Combining the results from the electrical structure model and fluid model of the SSAF,it is inferred that the fluid content in the high-conductivity crust reach up to 20%.When the fluid salinity is 10 wt%,the fluid content required to achieve the same high bulk conductivity reduces to 2%.A comparison of the electrical structure and focal depth of the Tan-Lu Fault Zone reveals that the collision between the low-viscosity fluids of the Mission Creek and Banning faults and the rigid Eastern Transverse Ranges contributes to the earthquake in the SSAF.展开更多
BACKGROUND Abnormal iron metabolism plays a critical role in paclitaxel(PTX)resistance in esophageal cancer cells.Qige San(QG)is a traditional Chinese herbal formula that is reported to improve short-term therapeutic ...BACKGROUND Abnormal iron metabolism plays a critical role in paclitaxel(PTX)resistance in esophageal cancer cells.Qige San(QG)is a traditional Chinese herbal formula that is reported to improve short-term therapeutic effects of esophageal cancer.AIM To investigate the effects and regulatory mechanisms involved in QG-targeted PTX-resistant esophageal cancer cells.METHODS Cell viability was assessed using the Cell Counting Kit-8 assay.Ferroptosis was evaluated by analyzing lipid reactive oxygen species accumulation and the Fe2+concentration in PTX-resistant esophageal cancer cells.Expression of ferroptosis regulators was measured by western blot.Network pharmacology analysis was employed to identify potential targets of QG in PTX-resistant esophageal cancer cells.RESULTS Treatment with QG significantly suppressed the viability,proliferation,and migration of PTX-resistant esophageal cancer cells and simultaneously induced ferroptosis.The network pharmacology analysis identified the phosphoinositide 3-kinase(PI3K)/protein kinase B signaling pathway as the potential target of QG in PTX-resistant esophageal cancer cells.Activation of the PI3K pathway notably reversed the ferroptosis of PTX-resistant esophageal cancer cells that was induced by QG.CONCLUSION QG could repress the resistance of esophageal cancer cells to PTX via targeting the PI3K signaling pathway.展开更多
In the last two decades,seismic resilience(SR)has been developed as a main concept for the assessment of the structural vulnerabilities of buildings and city centres.In particular,historical centers consist of adjacen...In the last two decades,seismic resilience(SR)has been developed as a main concept for the assessment of the structural vulnerabilities of buildings and city centres.In particular,historical centers consist of adjacent buildings organized in blocks with common characteristics and similar typologies.The paper proposes a meth-odology to quantify SR for urban regions,by overcoming the state of the art studies that focus on assessing the SR for singular buildings.In this regard,the presented methodology may calculate the SR of blocks of buildings for the assessment of recovery investments of historical city centers.The main idea is to assess the level of vulnerability by accurate 3D surveys and visual inspections in order to select empirical fragility curves.The proposed methodology was herein applied to the city center of San Marino,designated by UNESCO as a world heritage site.展开更多
Background:Atopic dermatitis is a chronic inflammatory skin disorder characterized by recurrent eczema-like rashes and severe itching.Taxi San is an external herbal formulation with the effects of clearing heat,drying...Background:Atopic dermatitis is a chronic inflammatory skin disorder characterized by recurrent eczema-like rashes and severe itching.Taxi San is an external herbal formulation with the effects of clearing heat,drying dampness,detoxifying,and relieving itching,making it suitable for treating acute and subacute dermatitis or eczema.Objectives:To evaluate the clinical efficacy of topical Taxi San in treating atopic dermatitis patients with dampness-heat syndrome and its inhibitory effect against Staphylococcus aureus(S.aureus)colonization.Methods:50 patients with atopic dermatitis were enrolled from the Dermatology Department of Shaanxi Provincial Hospital of Traditional Chinese Medicine,with bilateral symmetrical lesions selected as target sites.The control-side lesions were treated with boric acid solution wet compresses,while the treatment-side lesions received Taxi San solution wet compresses,both administered twice daily for 14 d.Clinical efficacy was evaluated using the Scoring Atopic Dermatitis(SCORAD),Investigator Global Assessment(IGA),Dermatology Life Quality Index/Children’s Dermatology Life Quality Index(DLQI/CDLQI),adverse events(AEs)and S.aureus colonization density,which were compared between the groups.The antibacterial efficacy of Taxi San was further investigated through in vitro antibacterial tests.Results:After 14 d of treatment with Taxi San,erythema and pimples were reduced on the treated sides.Additionally,the SCORAD,IGA,and DLQI/CDLQI scores showed significant decreases(P<0.05).S.aureus colonization on the treated sides declined markedly from 78%to 4.76%.Compared to the control sides,the reduction in S.aureus colonization following 14 d of Taxi San treatment was statistically significant(P<0.05).Furthermore,in vitro antibacterial assays demonstrated that the minimum inhibitory concentration of Taxi San against the seven tested S.aureus strains was 0.125 g/mL.Conclusions:Taxi San effectively reduces S.aureus colonization and ameliorates clinical symptoms in atopic dermatitis patients with dampness-heat syndrome,demonstrating high therapeutic potential and safety.展开更多
现代安防监控系统主要是指数字高清监控系统,一般可以分解为三大部分:前端数字高清摄像机、存储设备、传输设备。现在前端摄像机越来越倾向高清化、IP化,而存储及传输设备以数字视频的压缩、传输、存储和播放为核心,实现分级存储、集中...现代安防监控系统主要是指数字高清监控系统,一般可以分解为三大部分:前端数字高清摄像机、存储设备、传输设备。现在前端摄像机越来越倾向高清化、IP化,而存储及传输设备以数字视频的压缩、传输、存储和播放为核心,实现分级存储、集中管理。本文就安防监控系统实际的应用中采用数据挖掘技术,对如何架设规模化的SAN(Storage Area Network)存储区域网络来保存庞大的录像资料进行选择并提出推荐意见。展开更多
BACKGROUND Colorectal cancer(CRC)is a malignant tumor characterized by high global incidence and mortality rates.Contemporary therapeutic modalities remain limited by suboptimal efficacy and adverse effects,thereby ne...BACKGROUND Colorectal cancer(CRC)is a malignant tumor characterized by high global incidence and mortality rates.Contemporary therapeutic modalities remain limited by suboptimal efficacy and adverse effects,thereby necessitating the pursuit of more efficacious treatment strategies.Within traditional Chinese medicine,spleen deficiency is regarded as a central pathogenic mechanism in CRC,persisting throughout the entire disease course.AIM To elucidate the mechanism by which modified Yigong San confers therapeutic efficacy against CRC,potentially exerting its effects through apoptosis regulation mediated by the enhancer of zeste homolog 2(EZH2)/methyltransferase-like 3(METTL3)/SRY-box transcription factor 4(SOX4)axis.METHODS In the clinical study,CRC tissues and corresponding adjacent normal samples that fulfilled inclusion criteria were procured.Quantitative reverse transcription polymerase chain reaction was employed to determine the transcriptional expression of EZH2 and METTL3 mRNA.For in vitro experimentation,SW-480 cells were allocated into five experimental conditions:Control,control+serum,control+negative control,control+overexpressing-EZH2,and control+overexpressing-EZH2+serum.The mRNA expression levels of EZH2,METTL3,SOX4,B-cell lymphoma 2,and Bax across groups were quantified via quantitative reverse transcription polymerase chain reaction,while protein levels were assessed using western blot analysis.The presence of EZH2 binding sites within the METTL3 promoter region was verified through chromatin immunoprecipitation polymerase chain reaction.The optimal concentration of drug-containing serum(5%,10%,15%)was determined using the Cell Counting Kit-8 assay.Cell migratory ability was evaluated via scratch assays,and apoptotic activity was quantified by flow cytometry.RESULTS The clinical findings demonstrated significantly elevated transcriptional levels of METTL3 and EZH2 mRNA in tumor tissues compared to their adjacent normal counterparts(P<0.05).In vitro,cells treated with modified Yigong San exhibited a substantial downregulation of EZH2,METTL3,SOX4,B-cell lymphoma 2,and Bax mRNA and protein levels(P<0.05),relative to the control group.Apoptotic rates were markedly increased,while migratory capacity was significantly attenuated.Furthermore,in EZH2-overexpressing cells treated with modified Yigong San,similar reductions in both mRNA and protein levels of the aforementioned targets were observed(P<0.05),concomitant with enhanced apoptosis and reduced migration.Chromatin immunoprecipitation polymerase chain reaction analysis confirmed EZH2 occupancy at specific loci within the METTL3 promoter.CONCLUSION Modified Yigong San exhibits both preventive and therapeutic potential against CRC,likely mediated through the regulation of apoptosis via the EZH2/METTL3/SOX4 signaling pathway.展开更多
基金the Guangdong Provincial Basic and Applied Basic Research Project:Mechanistic Study on the Regulation of Inflammatory Microenvironment and Improvement of Ulcerative Colitis by Lingnan Traditional Medicine Ficus Pandurata Hance through Wilms'Tumor 1-associating Protein-Mediated RNA Methyltransferase Promoting Toll Like Receptor 4 m6A Modification(2023A1515011699)the Zhongshan Medical Research Project:Mechanistic Study on the Action of Xiahuo Pingwei San in the Treatment of Ulcerative Colitis(2022A020446)。
文摘OBJECTIVE:To evaluate the therapeutic effects of Xiahuo Pingwei San(夏藿平胃散,XHPWS)on ulcerative colitis(UC)in mice and to explore the underlying mechanisms through a network pharmacology approach.METHODS:Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS)was utilized to identify the chemical composition and authenticate the active constituents of XHPWS,ensuring rigorous quality control across batches.A dextran sulfate sodium(DSS)-induced UC model was established in C57BL/6 mice,which were treated with XHPWS in vivo.The efficacy against UC was assessed by measuring parameters such as body weight,disease activity index(DAI)scores,and colon length.Levels of inflammatory cytokines,including interleukin-6(IL-6),interleukin-1β(IL-1β),and tumor necrosis factor-alpha(TNF-α),in colonic tissue were evaluated using enzymelinked immunosorbent assay(ELISA).Histological analysis of colon sections was conducted using hematoxylin and eosin staining.A network pharmacology approach was employed to explore the mechanisms of XHPWS and to predict its potential targets in UC treatment.Predicted protein expressions in colonic tissue were validated using immune-ohistochemistry(IHC)and Western blotting techniques.RESULTS:XHPWS effectively alle via ted DSS-induced UC symptoms in mice,as evidenced by restored body weight,reduced colon shortening,and decreased DAI scores.Histopathological examination revealed that XHPWS significantly reduced intestinal inflammatory infiltration,restored intestinal epithelial permeability,and increased goblet cell count.Network pharmacology analysis identified 63 active compounds in XHPWS and suggested that it might target 35 potential proteins associated with UC treatment.Functional enrichment analysis indicated that the protective mechanism of XHPWS could be related to the advanced glycation end products-receptor for advanced glycation end products(AGE-RAGE)signaling pathway.Notably,quercetin,kaempferol,wogonin,and nobiletin,the main components of XHPWS,showed strong correlations with the core targets.Additionally,experimental validation demonstrated that XHPWS significantly decreased levels of inflammatory cytokines interleukin 6(IL-6),interleukin 1 beta(IL-1β),and tumor necrosis factor alpha(TNF-α)in UC mice,while downregulating the expression of proteins related to the AGE-RAGE pathway.CONCLUSION:Our study demonstrated that XHPWS effectively alle via tes colitis symptoms and inflammation in UC mice,potentially through the regulation of the AGE-RAGE pathway.These findings provide strong evidence for the therapeutic potential of XHPWS in UC treatment,thereby broadening its clinical applications.
文摘采用种子乳液聚合技术在聚丁二烯(PB)乳胶粒子上接枝共聚苯乙烯(St)和丙烯腈(AN)合成了一系列PB质量分数为40%的PB g SAN弹性体·研究了共聚单体中叔 十二烷基硫醇(TDDM)质量分数对SAN共聚物接枝行为、弹性体粒子结构及其动态力学性能的影响·结果发现,随着单体中TDDM质量分数的增加,SAN在PB上的接枝率和接枝效率降低,弹性体粒子中SAN包容结构的尺寸增大,在低温区的动态剪切模量下降·将其与SAN树脂共混后发现在共聚单体中引入TDDM后提高了弹性体粒子的增韧效率·
基金supported by the National Natural Science Foundation of China(42167023)the China Postdoctoral Science Foundation(2024MD753947)+1 种基金the National Natural Science Foundation of Inner Mongolia(2023QN04007,2022MS04009)the Basic Scientific Research Project of Institutions of Higher Learning in Inner Mongolia(JY20230090).
文摘The study of the electrical structure and fluid content of the southern San Andreas Fault(SSAF)plays a significant role in understanding the geological processes and earthquake genesis.The paper analyzes the Bahr skew and G-B decomposition from magnetotelluric sounding data in the SSAF to determine the strike direction is north-eastward 135°.Using the Nonlinear Conjugate Gradient algorithm,a reliable 2D electrical structure model was obtained.The Mission Creek and Banning faults,components of the SSAF,exhibit high conductivity within the crust,whereas the Eastern Transverse Ranges to the northeast of the fault show high resistivity characteristics.By integrating the modified Archie's law,the relations between conductivity,temperature,salinity,and fluid content were established,leading to conductivity-temperature and conductivity-fluid content relationships.Combining the results from the electrical structure model and fluid model of the SSAF,it is inferred that the fluid content in the high-conductivity crust reach up to 20%.When the fluid salinity is 10 wt%,the fluid content required to achieve the same high bulk conductivity reduces to 2%.A comparison of the electrical structure and focal depth of the Tan-Lu Fault Zone reveals that the collision between the low-viscosity fluids of the Mission Creek and Banning faults and the rigid Eastern Transverse Ranges contributes to the earthquake in the SSAF.
基金Supported by Zhejiang Traditional Chinese Medicine Administration,No.2024ZL944.
文摘BACKGROUND Abnormal iron metabolism plays a critical role in paclitaxel(PTX)resistance in esophageal cancer cells.Qige San(QG)is a traditional Chinese herbal formula that is reported to improve short-term therapeutic effects of esophageal cancer.AIM To investigate the effects and regulatory mechanisms involved in QG-targeted PTX-resistant esophageal cancer cells.METHODS Cell viability was assessed using the Cell Counting Kit-8 assay.Ferroptosis was evaluated by analyzing lipid reactive oxygen species accumulation and the Fe2+concentration in PTX-resistant esophageal cancer cells.Expression of ferroptosis regulators was measured by western blot.Network pharmacology analysis was employed to identify potential targets of QG in PTX-resistant esophageal cancer cells.RESULTS Treatment with QG significantly suppressed the viability,proliferation,and migration of PTX-resistant esophageal cancer cells and simultaneously induced ferroptosis.The network pharmacology analysis identified the phosphoinositide 3-kinase(PI3K)/protein kinase B signaling pathway as the potential target of QG in PTX-resistant esophageal cancer cells.Activation of the PI3K pathway notably reversed the ferroptosis of PTX-resistant esophageal cancer cells that was induced by QG.CONCLUSION QG could repress the resistance of esophageal cancer cells to PTX via targeting the PI3K signaling pathway.
文摘In the last two decades,seismic resilience(SR)has been developed as a main concept for the assessment of the structural vulnerabilities of buildings and city centres.In particular,historical centers consist of adjacent buildings organized in blocks with common characteristics and similar typologies.The paper proposes a meth-odology to quantify SR for urban regions,by overcoming the state of the art studies that focus on assessing the SR for singular buildings.In this regard,the presented methodology may calculate the SR of blocks of buildings for the assessment of recovery investments of historical city centers.The main idea is to assess the level of vulnerability by accurate 3D surveys and visual inspections in order to select empirical fragility curves.The proposed methodology was herein applied to the city center of San Marino,designated by UNESCO as a world heritage site.
基金supported by the Shaanxi Provincial Research and Innovation Team for Atopic Dermatitis of Traditional Chinese Medicine(No.TZKN-CXTD-02)the Key Industry Innovation Chain Project of Shaanxi Province(No.2021ZDLSF04-12).
文摘Background:Atopic dermatitis is a chronic inflammatory skin disorder characterized by recurrent eczema-like rashes and severe itching.Taxi San is an external herbal formulation with the effects of clearing heat,drying dampness,detoxifying,and relieving itching,making it suitable for treating acute and subacute dermatitis or eczema.Objectives:To evaluate the clinical efficacy of topical Taxi San in treating atopic dermatitis patients with dampness-heat syndrome and its inhibitory effect against Staphylococcus aureus(S.aureus)colonization.Methods:50 patients with atopic dermatitis were enrolled from the Dermatology Department of Shaanxi Provincial Hospital of Traditional Chinese Medicine,with bilateral symmetrical lesions selected as target sites.The control-side lesions were treated with boric acid solution wet compresses,while the treatment-side lesions received Taxi San solution wet compresses,both administered twice daily for 14 d.Clinical efficacy was evaluated using the Scoring Atopic Dermatitis(SCORAD),Investigator Global Assessment(IGA),Dermatology Life Quality Index/Children’s Dermatology Life Quality Index(DLQI/CDLQI),adverse events(AEs)and S.aureus colonization density,which were compared between the groups.The antibacterial efficacy of Taxi San was further investigated through in vitro antibacterial tests.Results:After 14 d of treatment with Taxi San,erythema and pimples were reduced on the treated sides.Additionally,the SCORAD,IGA,and DLQI/CDLQI scores showed significant decreases(P<0.05).S.aureus colonization on the treated sides declined markedly from 78%to 4.76%.Compared to the control sides,the reduction in S.aureus colonization following 14 d of Taxi San treatment was statistically significant(P<0.05).Furthermore,in vitro antibacterial assays demonstrated that the minimum inhibitory concentration of Taxi San against the seven tested S.aureus strains was 0.125 g/mL.Conclusions:Taxi San effectively reduces S.aureus colonization and ameliorates clinical symptoms in atopic dermatitis patients with dampness-heat syndrome,demonstrating high therapeutic potential and safety.
文摘现代安防监控系统主要是指数字高清监控系统,一般可以分解为三大部分:前端数字高清摄像机、存储设备、传输设备。现在前端摄像机越来越倾向高清化、IP化,而存储及传输设备以数字视频的压缩、传输、存储和播放为核心,实现分级存储、集中管理。本文就安防监控系统实际的应用中采用数据挖掘技术,对如何架设规模化的SAN(Storage Area Network)存储区域网络来保存庞大的录像资料进行选择并提出推荐意见。
基金Supported by Liaoning Provincial Science and Technology Department Project,No.2023JH2/101700149Open Fund Project of Liaoning University of Traditional Chinese Medicine,No.zyzx2205.
文摘BACKGROUND Colorectal cancer(CRC)is a malignant tumor characterized by high global incidence and mortality rates.Contemporary therapeutic modalities remain limited by suboptimal efficacy and adverse effects,thereby necessitating the pursuit of more efficacious treatment strategies.Within traditional Chinese medicine,spleen deficiency is regarded as a central pathogenic mechanism in CRC,persisting throughout the entire disease course.AIM To elucidate the mechanism by which modified Yigong San confers therapeutic efficacy against CRC,potentially exerting its effects through apoptosis regulation mediated by the enhancer of zeste homolog 2(EZH2)/methyltransferase-like 3(METTL3)/SRY-box transcription factor 4(SOX4)axis.METHODS In the clinical study,CRC tissues and corresponding adjacent normal samples that fulfilled inclusion criteria were procured.Quantitative reverse transcription polymerase chain reaction was employed to determine the transcriptional expression of EZH2 and METTL3 mRNA.For in vitro experimentation,SW-480 cells were allocated into five experimental conditions:Control,control+serum,control+negative control,control+overexpressing-EZH2,and control+overexpressing-EZH2+serum.The mRNA expression levels of EZH2,METTL3,SOX4,B-cell lymphoma 2,and Bax across groups were quantified via quantitative reverse transcription polymerase chain reaction,while protein levels were assessed using western blot analysis.The presence of EZH2 binding sites within the METTL3 promoter region was verified through chromatin immunoprecipitation polymerase chain reaction.The optimal concentration of drug-containing serum(5%,10%,15%)was determined using the Cell Counting Kit-8 assay.Cell migratory ability was evaluated via scratch assays,and apoptotic activity was quantified by flow cytometry.RESULTS The clinical findings demonstrated significantly elevated transcriptional levels of METTL3 and EZH2 mRNA in tumor tissues compared to their adjacent normal counterparts(P<0.05).In vitro,cells treated with modified Yigong San exhibited a substantial downregulation of EZH2,METTL3,SOX4,B-cell lymphoma 2,and Bax mRNA and protein levels(P<0.05),relative to the control group.Apoptotic rates were markedly increased,while migratory capacity was significantly attenuated.Furthermore,in EZH2-overexpressing cells treated with modified Yigong San,similar reductions in both mRNA and protein levels of the aforementioned targets were observed(P<0.05),concomitant with enhanced apoptosis and reduced migration.Chromatin immunoprecipitation polymerase chain reaction analysis confirmed EZH2 occupancy at specific loci within the METTL3 promoter.CONCLUSION Modified Yigong San exhibits both preventive and therapeutic potential against CRC,likely mediated through the regulation of apoptosis via the EZH2/METTL3/SOX4 signaling pathway.
