Syringin is found in the root of Acanthopanax senticosus(Rupr.Maxim.)Harms and belongs to the lignin chemical compound with many biological activities.In this study,we employed commercially available starting material...Syringin is found in the root of Acanthopanax senticosus(Rupr.Maxim.)Harms and belongs to the lignin chemical compound with many biological activities.In this study,we employed commercially available starting materials and accomplished the total synthesis of syringin in 5 steps with an overall yield of 58%.Palladium-catalyzed C(O)–C bond activation and subsequent cross coupling reaction is the key to construct syringin and its natural analogues.展开更多
Spinal cord injury typically causes corticospinal tract disruption.Although the disrupted corticospinal tract can self-regenerate to a certain degree,the underlying mechanism of this process is still unclear.N6-methyl...Spinal cord injury typically causes corticospinal tract disruption.Although the disrupted corticospinal tract can self-regenerate to a certain degree,the underlying mechanism of this process is still unclear.N6-methyladenosine(m^(6)A)modifications are the most common form of epigenetic regulation at the RNA level and play an essential role in biological processes.However,whether m^(6)A modifications participate in corticospinal tract regeneration after spinal cord injury remains unknown.We found that expression of methyltransferase 14 protein(METTL14)in the locomotor cortex was high after spinal cord injury and accompanied by elevated m^(6)A levels.Knockdown of Mettl14 in the locomotor cortex was not favorable for corticospinal tract regeneration and neurological recovery after spinal cord injury.Through bioinformatics analysis and methylated RNA immunoprecipitation-quantitative polymerase chain reaction,we found that METTL14 regulated Trib2 expression in an m^(6)A-regulated manner,thereby activating the mitogen-activated protein kinase pathway and promoting corticospinal tract regeneration.Finally,we administered syringin,a stabilizer of METTL14,using molecular docking.Results confirmed that syringin can promote corticospinal tract regeneration and facilitate neurological recovery by stabilizing METTL14.Findings from this study reveal that m^(6)A modification is involved in the regulation of corticospinal tract regeneration after spinal cord injury.展开更多
Codonopsis pilosula is a major Qi-tonifying medicinal herb,and its active composition is analyzed systematically.However,the relationship between its production origins and commodity specification grades with the acti...Codonopsis pilosula is a major Qi-tonifying medicinal herb,and its active composition is analyzed systematically.However,the relationship between its production origins and commodity specification grades with the active composition of C.pilosula lacks systematic research.This study integrates the HPLC and UV-Vis methodologies to evaluate the quality of C.pilosula from commodity specification grades and different origins,and it explores the correlation between ecological factors and production origins with active components.Here,network pharmacology is used to determine that lobetyolin,syringin,and tangshenoside I have potential efficacy in treating pulmonary fibrosis and oxidative stress.The HPLC and UV-Vis methods were employed to quantitatively analyse the levels of five active compounds from different origins and commodity specification grades.Ecological factors were collected from the different production origins with ArcGIS,and correlation analysis was conducted between these factors and the active components of C.pilosula to identify the key ecological influences that drive the accumulation of active compounds.Results showed that network pharmacology analyses indicated that the active components of C.pilosula,including lobetyolin,syringin,and tangshenoside I,bind to targets and exhibit antioxidant and antipulmonary fibrosis effects.Differences in the contents of active components across three commodity specification grades were not significant.The contents of active components in C.pilosula showed differences with varying origins,with the most variation observed in soluble sugar content,and notable variations are also observed in the levels of lobetyolin,syringin,and tangshenoside I,which could serve as potential biomarkers for different origins.Additionally,ecological factors influenced the accumulation of C.pilosula’s active components.The contents of soluble sugars and tangshenoside I were positively correlated with temperature and precipitation.Our study evaluated the active components of C.pilosula,and findings show that lobetyolin,syringin,and tangshenoside I have potential efficacy in treating pulmonary fibrosis and oxidative stress.The differences in the quality of C.pilosula across varying commodity specification grades are not significant.The different contents of C.pilosula across varying origins are significant,with soluble sugars and glycosides serving as potential markers for distinguishing C.pilosula from different origins.Moreover,ecological factors drove the accumulation of C.pilosula components.Soluble sugars and tangshenoside I content were particularly influenced by temperature and precipitation.Sand content and electrical conductivity significantly correlated with syringin,whereas organic carbon negatively influenced total flavonoids.This research provides a theoretical basis for the selection of the C.pilosula growing area and lays a foundation for the study of the C.pilosula quality standard.展开更多
基金supported by the Shanghai Institute of Materia Medica,the Chinese Academy of Sciences,the National Natural Science Foundation of China(21772211,21920102003)the Institutes for Drug Discovery and Development,Chinese Academy of Sciences(CASIMM0120163006)+2 种基金the Science and Technology Commission of Shanghai Miunicipality(17JC1405000,18431907100)the Program of Shanghai Academic Research Leader(19xD1424600)the National Science&Technology Major Project"Key New Drug Creation and Manufacturing Program",China(2018ZX09711002-006).
文摘Syringin is found in the root of Acanthopanax senticosus(Rupr.Maxim.)Harms and belongs to the lignin chemical compound with many biological activities.In this study,we employed commercially available starting materials and accomplished the total synthesis of syringin in 5 steps with an overall yield of 58%.Palladium-catalyzed C(O)–C bond activation and subsequent cross coupling reaction is the key to construct syringin and its natural analogues.
基金supported by the National Natural Science Foundation of China,Nos.82030071(to JH),82272495(to YC)Science and Technology Major Project of Changsha,No.kh2103008(to JH)Graduate Students’Independent Innovative Projects of Hunan Province,No.CX20230311(to YJ)。
文摘Spinal cord injury typically causes corticospinal tract disruption.Although the disrupted corticospinal tract can self-regenerate to a certain degree,the underlying mechanism of this process is still unclear.N6-methyladenosine(m^(6)A)modifications are the most common form of epigenetic regulation at the RNA level and play an essential role in biological processes.However,whether m^(6)A modifications participate in corticospinal tract regeneration after spinal cord injury remains unknown.We found that expression of methyltransferase 14 protein(METTL14)in the locomotor cortex was high after spinal cord injury and accompanied by elevated m^(6)A levels.Knockdown of Mettl14 in the locomotor cortex was not favorable for corticospinal tract regeneration and neurological recovery after spinal cord injury.Through bioinformatics analysis and methylated RNA immunoprecipitation-quantitative polymerase chain reaction,we found that METTL14 regulated Trib2 expression in an m^(6)A-regulated manner,thereby activating the mitogen-activated protein kinase pathway and promoting corticospinal tract regeneration.Finally,we administered syringin,a stabilizer of METTL14,using molecular docking.Results confirmed that syringin can promote corticospinal tract regeneration and facilitate neurological recovery by stabilizing METTL14.Findings from this study reveal that m^(6)A modification is involved in the regulation of corticospinal tract regeneration after spinal cord injury.
基金funded by the National Key R&D Plan(2022YFC3501804)Fundamental Research Funds for the Central Public Welfare Research Institutes(No.ZZ13-YQ-049)National Natural Science Foundation of China(ZXKT22001).
文摘Codonopsis pilosula is a major Qi-tonifying medicinal herb,and its active composition is analyzed systematically.However,the relationship between its production origins and commodity specification grades with the active composition of C.pilosula lacks systematic research.This study integrates the HPLC and UV-Vis methodologies to evaluate the quality of C.pilosula from commodity specification grades and different origins,and it explores the correlation between ecological factors and production origins with active components.Here,network pharmacology is used to determine that lobetyolin,syringin,and tangshenoside I have potential efficacy in treating pulmonary fibrosis and oxidative stress.The HPLC and UV-Vis methods were employed to quantitatively analyse the levels of five active compounds from different origins and commodity specification grades.Ecological factors were collected from the different production origins with ArcGIS,and correlation analysis was conducted between these factors and the active components of C.pilosula to identify the key ecological influences that drive the accumulation of active compounds.Results showed that network pharmacology analyses indicated that the active components of C.pilosula,including lobetyolin,syringin,and tangshenoside I,bind to targets and exhibit antioxidant and antipulmonary fibrosis effects.Differences in the contents of active components across three commodity specification grades were not significant.The contents of active components in C.pilosula showed differences with varying origins,with the most variation observed in soluble sugar content,and notable variations are also observed in the levels of lobetyolin,syringin,and tangshenoside I,which could serve as potential biomarkers for different origins.Additionally,ecological factors influenced the accumulation of C.pilosula’s active components.The contents of soluble sugars and tangshenoside I were positively correlated with temperature and precipitation.Our study evaluated the active components of C.pilosula,and findings show that lobetyolin,syringin,and tangshenoside I have potential efficacy in treating pulmonary fibrosis and oxidative stress.The differences in the quality of C.pilosula across varying commodity specification grades are not significant.The different contents of C.pilosula across varying origins are significant,with soluble sugars and glycosides serving as potential markers for distinguishing C.pilosula from different origins.Moreover,ecological factors drove the accumulation of C.pilosula components.Soluble sugars and tangshenoside I content were particularly influenced by temperature and precipitation.Sand content and electrical conductivity significantly correlated with syringin,whereas organic carbon negatively influenced total flavonoids.This research provides a theoretical basis for the selection of the C.pilosula growing area and lays a foundation for the study of the C.pilosula quality standard.
