目的探讨斑蝥素(Cantharidin)对结直肠癌SW480细胞增殖、侵袭及凋亡的影响,并初步阐明其潜在分子机制。方法采用体外培养的SW480细胞,通过MTS法和克隆形成实验检测细胞活力与长期增殖能力;利用Transwell实验评估细胞侵袭能力;运用流式...目的探讨斑蝥素(Cantharidin)对结直肠癌SW480细胞增殖、侵袭及凋亡的影响,并初步阐明其潜在分子机制。方法采用体外培养的SW480细胞,通过MTS法和克隆形成实验检测细胞活力与长期增殖能力;利用Transwell实验评估细胞侵袭能力;运用流式细胞术分析细胞凋亡与周期分布;并采用Western Blot技术检测PI3K/AKT信号通路关键蛋白的表达及其磷酸化水平。结果与对照组相比,斑蝥素能够剂量依赖性地显著抑制SW480细胞的增殖(48 h IC50为11.63μM)、克隆形成及侵袭能力。此外,斑蝥素可诱导细胞周期阻滞于G2/M期,并显著促进细胞凋亡。研究表明,斑蝥素处理能显著抑制PI3K与AKT的磷酸化,下调总PI3K、总AKT、CDK4、MMP9及抗凋亡蛋白Bcl-2的表达,同时上调促凋亡蛋白Bax的表达。结论斑蝥素可通过抑制PI3K/AKT信号通路,进而抑制结直肠癌SW480细胞的增殖与侵袭,并诱导其凋亡。展开更多
Objective:To evaluate the quality of Lygodium japonicum(Thunb.)Sw.(L.japonicum,Hai Jin Sha)by comparing its components without stewed(W)and stewed(S)using ultra-high-performance liquid chromatography(UHPLC)and chemome...Objective:To evaluate the quality of Lygodium japonicum(Thunb.)Sw.(L.japonicum,Hai Jin Sha)by comparing its components without stewed(W)and stewed(S)using ultra-high-performance liquid chromatography(UHPLC)and chemometric analysis.Additionally,network pharmacology was employed to investigate the possible mechanisms of action of L.japonicum in the urinary calculi(UC)treatment.Methods:A fingerprinting method was established to identify components through UHPLC-tandem mass spectrometry.Chemometric techniques were used to compare the L.japonicum extraction methods.Furthermore,various network pharmacological approaches were used to identify and analyze the potential targets of the identified components in relation to UC.Results:The W and S extracts were distributed into two distinct clusters.Significant differences in the levels of protocatechuic aldehyde,caffeic acid,and p-coumaric acid were observed between S and W.Network pharmacology analysis revealed that the primary targets of L.japonicum in the UC treatment were serum albumin and epidermal growth factor receptors,with potential active components including protocatechuic acid and caffeic acid.Conclusion:This study comprehensively examined the therapeutic components of L.japonicum before and after boiling,shedding light on its potential mechanisms of action in UC treatment.These findings offer valuable insights into the development and utilization of L.japonicum resources.展开更多
文摘目的探讨斑蝥素(Cantharidin)对结直肠癌SW480细胞增殖、侵袭及凋亡的影响,并初步阐明其潜在分子机制。方法采用体外培养的SW480细胞,通过MTS法和克隆形成实验检测细胞活力与长期增殖能力;利用Transwell实验评估细胞侵袭能力;运用流式细胞术分析细胞凋亡与周期分布;并采用Western Blot技术检测PI3K/AKT信号通路关键蛋白的表达及其磷酸化水平。结果与对照组相比,斑蝥素能够剂量依赖性地显著抑制SW480细胞的增殖(48 h IC50为11.63μM)、克隆形成及侵袭能力。此外,斑蝥素可诱导细胞周期阻滞于G2/M期,并显著促进细胞凋亡。研究表明,斑蝥素处理能显著抑制PI3K与AKT的磷酸化,下调总PI3K、总AKT、CDK4、MMP9及抗凋亡蛋白Bcl-2的表达,同时上调促凋亡蛋白Bax的表达。结论斑蝥素可通过抑制PI3K/AKT信号通路,进而抑制结直肠癌SW480细胞的增殖与侵袭,并诱导其凋亡。
文摘顺序任务流(sequential task flow,STF)将对共享数据的访问表示为任务之间的依赖关系,STF运行时系统通过任务构造、依赖分析和任务依赖图(task dependence graph,TDG)生成、任务调度实现异步并行,这3个环节的开销直接影响并行程序的性能.目前以STF为核心的AceMesh运行时系统,在SW39000处理器上仅使用单主核构图、多从核执行的方式.然而,SW39000处理器离散访存性能较弱,细粒度任务构图离散访存增多,构图更容易成为瓶颈.对此,提出了一种利用多从核辅助主核进行构图的算法.首先,分析在依赖分析和TDG生成过程中的并行性,在SW39000处理器上实现了一种基于胖任务依赖图(fatTDG)的多核辅助并行构图算法PFBH(parallelized fatTDG building algorithm with helpers)并进行优化.其次,针对线程间的主存资源竞争问题,提出构图与执行并行中从核资源调节方法及参数选择.最终,在5类典型应用下进行实验测试.与单核串行构图系统相比,在细粒度任务场景下最高加速为1.75倍;与SW39000处理器上的OpenACC模型相比,AceMesh最高可达2倍加速.
基金supported by Ministry of Industry and Information Technology of the People's Republic of China 2022 Industrial Technology Basic Public Service Platform Project-Traditional Chinese Medicine Whole Industry Chain Quality and Technology Service Platform(2022-230-221)Foshan Nanhai District Key Area Science and Technology Research Project[Nanke(2023)20-18].
文摘Objective:To evaluate the quality of Lygodium japonicum(Thunb.)Sw.(L.japonicum,Hai Jin Sha)by comparing its components without stewed(W)and stewed(S)using ultra-high-performance liquid chromatography(UHPLC)and chemometric analysis.Additionally,network pharmacology was employed to investigate the possible mechanisms of action of L.japonicum in the urinary calculi(UC)treatment.Methods:A fingerprinting method was established to identify components through UHPLC-tandem mass spectrometry.Chemometric techniques were used to compare the L.japonicum extraction methods.Furthermore,various network pharmacological approaches were used to identify and analyze the potential targets of the identified components in relation to UC.Results:The W and S extracts were distributed into two distinct clusters.Significant differences in the levels of protocatechuic aldehyde,caffeic acid,and p-coumaric acid were observed between S and W.Network pharmacology analysis revealed that the primary targets of L.japonicum in the UC treatment were serum albumin and epidermal growth factor receptors,with potential active components including protocatechuic acid and caffeic acid.Conclusion:This study comprehensively examined the therapeutic components of L.japonicum before and after boiling,shedding light on its potential mechanisms of action in UC treatment.These findings offer valuable insights into the development and utilization of L.japonicum resources.
