通过开源多物理场模拟与设计集成软件平台SU2(Stanford University Unstructured),使用格点型有限体积格式,研究了来流攻角12°,雷诺数为Re=2×10^6下NACA0012半矩形翼扰流问题。通过涡向量Rortex/Liutex揭示了方形翼尖纯刚体...通过开源多物理场模拟与设计集成软件平台SU2(Stanford University Unstructured),使用格点型有限体积格式,研究了来流攻角12°,雷诺数为Re=2×10^6下NACA0012半矩形翼扰流问题。通过涡向量Rortex/Liutex揭示了方形翼尖纯刚体旋转涡的形成与发展,次涡与主涡的合并,并与涡量进行了比较。结果表明:钝体矩形翼翼尖的两个锋利奇异边产生Rortex/Liutex涡,且Rortex/Liutex表示的次涡与主涡的合并与相互作用要晚于涡量;此外,除了奇异边外的翼尖侧面,虽具有较高涡量,但贡献了流体的剪切或拉伸。展开更多
[Objective] Using molecular biotechnology to clone the sus scrofa GPX2 gene. [Method] Using total RNA of sus scrofa duodenum as template, degenerated primer pairs were designed according to the homology alignment anal...[Objective] Using molecular biotechnology to clone the sus scrofa GPX2 gene. [Method] Using total RNA of sus scrofa duodenum as template, degenerated primer pairs were designed according to the homology alignment analysis of GPX2 gene of human, rat, mouse, dog and cattle. A sus scrofa GPX2 gene sequence of 330 bp was obtained by RT-PCR application method. Primes were designed respectively according to the known sequence, sus scrofa GPX2 gene was isolated and cloned by 3-RACE and 5-RACE method and analyzed the gene sequence. [Result] A mRNA sequence of 924 bp was successfully cloned and isolated in this research. This sequence contained complete 3＇end and had higher sequence homology with human,mouse,cattle and dog GPX2 gene, and there was codon called TGA which encoding Sec on the position of No. 114-116 gene. [Conclusion] Sequence alignment analysis showed that the cloned gene was sus scrofa GPX2 gene （ NCBI GenBank database, the sequence number was D098982）.展开更多
文摘通过开源多物理场模拟与设计集成软件平台SU2(Stanford University Unstructured),使用格点型有限体积格式,研究了来流攻角12°,雷诺数为Re=2×10^6下NACA0012半矩形翼扰流问题。通过涡向量Rortex/Liutex揭示了方形翼尖纯刚体旋转涡的形成与发展,次涡与主涡的合并,并与涡量进行了比较。结果表明:钝体矩形翼翼尖的两个锋利奇异边产生Rortex/Liutex涡,且Rortex/Liutex表示的次涡与主涡的合并与相互作用要晚于涡量;此外,除了奇异边外的翼尖侧面,虽具有较高涡量,但贡献了流体的剪切或拉伸。
文摘[Objective] Using molecular biotechnology to clone the sus scrofa GPX2 gene. [Method] Using total RNA of sus scrofa duodenum as template, degenerated primer pairs were designed according to the homology alignment analysis of GPX2 gene of human, rat, mouse, dog and cattle. A sus scrofa GPX2 gene sequence of 330 bp was obtained by RT-PCR application method. Primes were designed respectively according to the known sequence, sus scrofa GPX2 gene was isolated and cloned by 3-RACE and 5-RACE method and analyzed the gene sequence. [Result] A mRNA sequence of 924 bp was successfully cloned and isolated in this research. This sequence contained complete 3＇end and had higher sequence homology with human,mouse,cattle and dog GPX2 gene, and there was codon called TGA which encoding Sec on the position of No. 114-116 gene. [Conclusion] Sequence alignment analysis showed that the cloned gene was sus scrofa GPX2 gene （ NCBI GenBank database, the sequence number was D098982）.
