Plantshave evolvedvariousmechanismsto interact withmicroorganisms,which help them acquire nutrients from the soil and enhance their tolerance to environmental stresses.One of the most widespread mutualistic interactio...Plantshave evolvedvariousmechanismsto interact withmicroorganisms,which help them acquire nutrients from the soil and enhance their tolerance to environmental stresses.One of the most widespread mutualistic interactions is arbuscular mycorrhizal(AM)symbiosis,which is formed by 80%-90%of terrestrial plants in association with AM fungi.In AM symbiosis,plants acquire mineral nutrients from the fungi in exchange for fatty acids and sugars that are produced during photosynthesis(Jiang et al.,2017).展开更多
A steady rise in the overall population is creating an overburden on crops due to their global demand.On the other hand,given the current climate change and population growth,agricultural practices established during ...A steady rise in the overall population is creating an overburden on crops due to their global demand.On the other hand,given the current climate change and population growth,agricultural practices established during the Green Revolution are no longer viable.Consequently,innovative practices are the prerequisite of the time struggle with the rising global food demand.The potential of nanotechnology to reduce the phytotoxic effects of these ecological restrictions has shown significant promise.Nanoparticles(NPs)typically enhance plant resilience to stressors by fortifying the physical barrier,optimizing photosynthesis,stimulating enzymatic activity for defense,elevating the concentration of stress-resistant compounds,and activating the expression of genes associated with defense mechanisms.In this review,we thoroughly cover the uptake and translocations of NPs crops and their potential valuable functions in enhancing plant growth and development at different growth stages.Additionally,we addressed how NPs improve plant resistance to biotic and abiotic stress.Generally,this review presents a thorough understanding of the significance of NPs in plants and their prospective value for plant antioxidant and crop development.展开更多
The study aimed to address the issue of elevated residual stress levels in dissimilar girth welds of cast steel joints.To achieve this,the hybrid welding technology,which yields high welding speeds while simultaneousl...The study aimed to address the issue of elevated residual stress levels in dissimilar girth welds of cast steel joints.To achieve this,the hybrid welding technology,which yields high welding speeds while simultaneously reducing residual stresses,has been introduced.This study utilizes a numerical simulation method to investigate the temperature and residual stress field in the hybrid welding of G20Mn5 casting-Q355 low-alloy steel welded pipe.A com-parison of the findings of this study with those of other welding processes revealed the technological advantages of hybrid welding.The research outcomes show that due to geometric discontinuities and material differences,the temperature field of the joint exhibits uneven distribution characteristics,and the peak temperatures on the Q355 steel side exceeds those on the G20Mn5 steel side.An evident stress gra-dient is present in the residual stress field of the joint post-welding,with peak stress located at the weld root on the Q355 steel.Compared with arc welding,the hybrid welding leads to decreased residual stresses and deformation,with high stress outside the heat-affected zone diminishing rapidly.Furthermore,it significantly improves the welding efficiency.This study elucidates the distribution and underlying causes of thermal and residual stress fields in dissimilar girth welds.This serves as a foundation for the application of hybrid welding technology in welded cast steel joints.展开更多
Wear is a prevalent issue across various industries. Spherical fused tungsten carbide (sFTC) reinforced nickel-aluminum bronze (NAB) matrix composite surface deposits have shown remarkable potential in mitigating wear...Wear is a prevalent issue across various industries. Spherical fused tungsten carbide (sFTC) reinforced nickel-aluminum bronze (NAB) matrix composite surface deposits have shown remarkable potential in mitigating wear by approximately 80%. However, the performance of these sFTC/NAB composite surface deposits is determined by their residual stress state, and the precise macroscopic and microscopic residual stresses within these composites have yet to be clearly established. To address this gap, we employed neutron diffraction to measure the residual stresses in the sFTC/NAB composite surface deposits and re-melted NAB samples produced via laser melt injection. Significant residual stresses were determined. The maximum tensile macro residual stress appears approximately 1-1.5 mm below the composite layer. Residual stresses accumulate with an increasing number of laser process tracks. The maximum tensile macro residual stress in the three-track samples reaches about 350 MPa. Preheating the base plate significantly reduces the levels of macroscopic residual stress. The WC phase displayed significant compressive thermal misfit residual stress magnitude, while the Cu matrix exhibited tensile thermal misfit residual stress. Preheating the base plate does not reduce microscopic thermal misfit residual stress levels. In addition, a finite element model was built to investigate temperature and residual stresses in the re-melted NAB samples. The predicted temperature history and residual stress agree with the experimental results.展开更多
Glutathione S-transferases (GSTs) represent a large and diverse enzyme family ubiquitously distributed across the plant kingdom. These proteins catalyze the conjugation of glutathione (GSH) with electrophilic substrat...Glutathione S-transferases (GSTs) represent a large and diverse enzyme family ubiquitously distributed across the plant kingdom. These proteins catalyze the conjugation of glutathione (GSH) with electrophilic substrates in response to various stress conditions. Beyond their role in stress adaptation, certain GSTs are integral regulators of plant growth and development, contributing to a range of physiological processes. Most GST proteins exhibit dual enzymatic activities, functioning as both transferases and peroxidases, which enables their involvement in diverse cellular processes, including detoxification and stress responses. Recent advancements, particularly in X-ray crystallography, have enabled detailed structural analysis of GST proteins, significantly enhancing our understanding of their biological functions. This review offers a comprehensive overview of the classification and structural characteristics of GSTs in plants. It also highlights recent findings on their roles in plant growth and development, cell signaling, catalytic transport, and stress tolerance. Furthermore, key scientific challenges related to GSTs are discussed, focusing on their potential applications in agriculture. These insights aim to facilitate the screening of functional GST genes and support molecular breeding efforts across diverse crop species.展开更多
Landslides frequently occurred in Jurassic red strata in the Three Gorges Reservoir(TGR)region in China.The Jurassic strata consist of low mechanical strength and poor permeability of weak silty mudstone layer,which m...Landslides frequently occurred in Jurassic red strata in the Three Gorges Reservoir(TGR)region in China.The Jurassic strata consist of low mechanical strength and poor permeability of weak silty mudstone layer,which may cause slope instability during rainfall.In order to understand the strength behavior of Jurassic silty mudstone shear zone,the so-called Shizibao landslide located in Guojiaba Town,Zigui County,Three Gorges Reservoir(TGR)in China is selected as a case study.The shear strength of the silty mudstone shear zone is strongly influenced by both the water content and the normal stress.Therefore,a series of drained ring shear tests were carried out by varying the water contents(7%,12%,17%,and 20%,respectively)and normal stresses(200,300,400,and 500 kPa,respectively).The result revealed that the residual friction coefficient and residual friction angle were power function relationships with water content and normal stress.The peak cohesion of the silty mudstone slip zone increased with water content to a certain limit,above which the cohesion decreased.In contrast,the residual cohesion showed the opposite trend,indicating the cohesion recovery above a certain limit of water content.However,both the peak and residual friction angle of the silty mudstone slip zone were observed to decrease steadily with increased water content.Furthermore,the macroscopic morphological features of the shear surface showed that the sliding failure was developed under high normal stress at low water content,while discontinuous sliding surface and soil extrusion were occurred when the water content increased to a saturated degree.The localized liquefaction developed by excess pore water pressure reduced the frictional force within the shear zone.Finally,the combined effects of the slope excavation and precipitation ultimately lead to the failure of the silty mudstone slope;however,continuous rainfall is the main factor triggering sliding.展开更多
Heat shock transcription factors(Hsfs)have important roles during plant growth and development and responses to abiotic stresses.The identification and func-tion of Hsf genes have been thoroughly studied in various he...Heat shock transcription factors(Hsfs)have important roles during plant growth and development and responses to abiotic stresses.The identification and func-tion of Hsf genes have been thoroughly studied in various herbaceous plant species,but not woody species,especially Phoebe bournei,an endangered,unique species in China.In this study,17 members of the Hsf gene family were identi-fied from P.bournei using bioinformatic methods.Phyloge-netic analysis indicated that PbHsf genes were grouped into three subfamilies:A,B,and C.Conserved motifs,three-dimensional structure,and physicochemical properties of the PbHsf proteins were also analyzed.The structure of the PbHsf genes varied in the number of exons and introns.Pre-diction of cis-acting elements in the promoter region indi-cated that PbHsf genes are likely involved in responses to plant hormones and stresses.A collinearity analysis dem-onstrated that expansions of the PbHsf gene family mainly take place via segmental duplication.The expression levels of PbHsf genes varied across different plant tissues.On the basis of the expression profiles of five representative PbHsf genes during heat,cold,salt,and drought stress,PbHsf pro-teins seem to have multiple functions depending on the type of abiotic stress.This systematic,genome-wide investigation of PbHsf genes in P.bournei and their expression patterns provides valuable insights and information for further func-tional dissection of Hsf proteins in this endangered,unique species.展开更多
Large 2219 Al-Cu alloy aerospace integral components suffer from long-term stress relaxation aging(SRA)due to complex temperature and stress loads during aging treatment/forming and service process,which makes it diff...Large 2219 Al-Cu alloy aerospace integral components suffer from long-term stress relaxation aging(SRA)due to complex temperature and stress loads during aging treatment/forming and service process,which makes it difficult to ensure their appropriate residual stress and excellent mechanical and service prop-erties.However,the research is limited to a thorough understanding of macroscopic and microscopic features and underlying mechanisms of the long-term SRA under multivariable aging conditions.There-fore,this study investigated macroscopic and microscopic features of long-term SRA under different tem-peratures(120 ℃ to 190 ℃),initial stress levels(100 MPa to 250 MPa)and durations(0 h to 50 h)through stress relaxation curves,metallographic traits,Vickers hardness,tensile performance,disloca-tions and phases of precipitation.On the basis of experimental outcomes,the comprehensive mecha-nisms beneath SRA were unraveled through dislocation theory,multiphase strengthening mechanisms and thermodynamics,where the interplays of stress relaxation behavior with age-hardening response were taken into consideration.The results showed elevations in the rate of stress reduction as the tem-perature and initial stress rose.At an initial stress greater than the yield stress of alloy,a marked in-crease in stress relaxation was found,and the mechanisms transform from the intragranular motion of dislocations and diffusion of grain boundaries to the intragranular and intergranular motion of disloca-tions and migration of grain boundaries.The stress reduction rate rose sharply when the temperature exceeded 175 ℃,and the dislocation movement mechanisms transform from gliding to climbing of dislo-cations.Stress relaxation is in nature progressive transformation of strain from elastic into a permanently inelastic state via the motion of dislocations,leading to the decrease of movable dislocations and the increase of immovable dislocations with more stable configurations.The age hardening is mainly deter-mined by precipitation strengthening,supplementarily by dislocation strengthening,and obvious stress orientation effect(SOE)of G.P.zones and θ"phases degenerates strengthening effect.The interplay be-tween stress relaxation behavior and age-hardening response influences the thermal-mechanical coupling SRA of 2219 Al-Cu alloy,which depends fundamentally on the motion of dislocations and their interplay with precipitated phases.This is a thermal activation process concerning the interplay between internal(age-hardening resistance)stress and external(initial)stress.The initial energy of elastic strain offers Gibbs free energy as the SRA driver,and a steady state of stress relaxation is attained with the lowest energy of elastic strain.These findings provide valuable insights into exploring innovative aging treat-ment/forming for optimizing residual stress,mechanical performance and service property in a synergistic manner.展开更多
In crop plants, various environmental stresses affect the balance of carbon, nitrogen, and phosphorus(C:N:P), leading to biochemical and physiological alterations and reductions in yield. Silicon(Si) is a beneficial e...In crop plants, various environmental stresses affect the balance of carbon, nitrogen, and phosphorus(C:N:P), leading to biochemical and physiological alterations and reductions in yield. Silicon(Si) is a beneficial element that alleviates plant stress. Most studies involving silicon have focused on physiological responses, such as improvements in photosynthetic processes, water use efficiency, and antioxidant defense systems. But recent research suggests that stressed plants facing either limited or excessive resources(water, light, nutrients, and toxic elements), strategically employ Si to maintain C:N:P homeostasis, thereby minimizing biomass losses. Understanding the role of Si in mitigating the impact of abiotic stresses on plants by regulating C:N:P homeostasis holds great potential for advancing sustainable agricultural practices in crop production. This review presents recent advances in characterizing the influence of environmental stresses on C:N:P homeostasis, as well as the role of Si in preserving C:N:P equilibrium and attenuating biological damage associated with abiotic stress. It underscores the beneficial effects of Si in sustaining C:N:P homeostasis and increasing yield via improved nutritional efficiency and stress mitigation.展开更多
Boulder spacing in mountain rivers and near-wake flow zones within the boulder array is very useful for fish habitat and growth of aquatic organisms.The present study aims to investigate how the boulder array and spac...Boulder spacing in mountain rivers and near-wake flow zones within the boulder array is very useful for fish habitat and growth of aquatic organisms.The present study aims to investigate how the boulder array and spacing influence the near-bed flow structures in a gravel-bed stream.Boulders are staggered over a gravel-bed stream with three different inter-boulder spacing namely(a)large(b)medium and(c)small spacing.An acoustic Doppler velocimeter was used for flow measurements in a rectangular channel and the results were compared with those acquired from numerical simulation.The time-averaged velocity profiles at the near-wake flow zones of boulders experience maximum flow retardation which is an outcome of the boulder-induced form roughness.The ratio of velocity differences associated to form and skin roughness and its positive magnitude reveals the dominance of form roughness closest to the boulders.Form roughness computed is 1.75 to 2 times higher than the skin roughness at the near-wake flow region.In particular,the collective immobile boulders placed at different inter-boulder spacings developed high and low bed shear stresses closest to the boulders.The low bed shear stresses characterised by a secondary peak developed at the trough location of the boulders is attributed to the skin shear stress.Further,the spatial averaging of time-averaged flow quantities gives additional impetus to present an improved illustration of fluid shear stresses.The formation of form-induced shear stress is estimated to be 17%to 23%of doubleaveraged Reynolds shear stress and partially compensates for the damping of time-averaged Reynolds shear stress in the interfacial sub-layer.The quadrant analysis of spatial velocity fluctuations depicts that the form-induced shear stresses are dominant in the interfacial sub-layer and have no significance above the gravel-bed surface.展开更多
Purpose-The experiments of this study investigated the effect of the subgrade degree of saturation on the value of the stresses generated on the surface and the middle(vertical and lateral stresses).The objectives of ...Purpose-The experiments of this study investigated the effect of the subgrade degree of saturation on the value of the stresses generated on the surface and the middle(vertical and lateral stresses).The objectives of this study can be identified by studying the effect of subgrade layer degree of saturation variation,load amplitude and load frequency on the transmitted stresses through the ballast layer to the subgrade layer and the stress distribution inside it and investigating the excess pore water pressure development in the clay layer in the case of a fully saturated subgrade layer and the change in matric suction in the case of an unsaturated subgrade layer.Design/methodology/approach-Thirty-six laboratory experiments were conducted using approximately half-scale replicas of real railways,with an iron box measuring 1.5×1.031.0 m.Inside the box,a 0.5 m thick layer of clay soil representing the base layer was built.Above it is a 0.2 m thick ballast layer made of crushed stone,and on top of that is a 0.8 m long rail line supported by three 0.9 m(0.1×0.1 m)slipper beams.The subgrade layer has been built at the following various saturation levels:100,80,70 and 60%.Experiments were conducted with various frequencies of 1,2 and 4 Hz with load amplitudes of 15,25 and 35 kN.Findings-The results of the study demonstrated that as the subgrade degree of saturation decreased from 100 to 60%,the ratio of stress in the lateral direction to stress in the vertical direction generated in the middle of the subgrade layer decreased as well.On average,this ratio changed from approximately 0.75 to approximately 0.65.Originality/value-The study discovered that as the test proceeded and the number of cycles increased,the value of negative water pressure(matric suction)in the case of unsaturated subgrade soils declined.The frequency of loads had no bearing on the ratio of decline in matric suction values,which was greater under a larger load amplitude than a lower one.As the test progressed(as the number of cycles increased),the matric suction dropped.For larger load amplitudes,there is a greater shift in matric suction.The change in matric suction is greater at higher saturation levels than it is at lower saturation levels.Furthermore,it is seen that the load frequency value has no bearing on how the matric suction changes.For all load frequencies and subgrade layer saturation levels,the track panel settlement rises with the load amplitude.Higher load frequency and saturation levels have a greater impact.展开更多
High-purity(HP)magnesium(Mg)has emerged as a promising biomaterial for supporting functional bone tissue.Our previous study found that mechanical stresses and the surrounding fibrotic tissue(subcuta-neous)both play cr...High-purity(HP)magnesium(Mg)has emerged as a promising biomaterial for supporting functional bone tissue.Our previous study found that mechanical stresses and the surrounding fibrotic tissue(subcuta-neous)both play crucial roles in the degradation of HP Mg.However,due to challenges in the degradation and regeneration process in vivo,it remains unclear how stress affects HP Mg degradation in bone en-vironments,limiting its further application.In this study,novel loading devices were designed and the effects of tensile and compressive stresses on HP Mg degradation in vivo and in vitro bone environments were quantitatively analyzed.In addition,bone osteointegration around HP Mg was explored preliminar-ily.Tensile stress increases the degradation rate of HP Mg in vivo and in vitro.HP Mg degradation in vivo is more sensitive to stress factors than in vitro,but the sensitivity decreases with corrosion time.The volume loss rate of HP Mg is multilinear with the applied stress and degradation time.The volume of bone tissue surrounding HP Mg is larger in the no-stress group compared to the stressed groups,which is more pronounced with increasing implantation time.These results provide valuable insights for optimiz-ing the design of HP Mg-based implants considering load conditions.This will help to achieve a balance between the degradation rate of the implant and the regeneration rate of the surrounding bone.展开更多
The present study establishes a new estimation model using an artificial neural network(ANN) to predict the mechanical properties of the AISI 1035 alloy.The experiments were designed based on the L16 orthogonal array ...The present study establishes a new estimation model using an artificial neural network(ANN) to predict the mechanical properties of the AISI 1035 alloy.The experiments were designed based on the L16 orthogonal array of the Taguchi method.A proposed numerical model for predicting the correlation of mechanical properties was supplemented with experimental data.The quenching process was conducted using a cooling medium called “nanofluids”.Nanoparticles were dissolved in a liquid phase at various concentrations(0.5%,1%,2.5%,and 5% vf) to prepare the nanofluids.Experimental investigations were done to assess the impact of temperature,base fluid,volume fraction,and soaking time on the mechanical properties.The outcomes showed that all conditions led to a noticeable improvement in the alloy's hardness which reached 100%,the grain size was refined about 80%,and unwanted residual stresses were removed from 50 to 150 MPa.Adding 5% of CuO nanoparticles to oil led to the best grain size refinement,while adding 2.5% of Al_(2)O_(3) nanoparticles to engine oil resulted in the greatest compressive residual stress.The experimental variables were used as the input data for the established numerical ANN model,and the mechanical properties were the output.Upwards of 99% of the training network's correlations seemed to be positive.The estimated result,nevertheless,matched the experimental dataset exactly.Thus,the ANN model is an effective tool for reflecting the effects of quenching conditions on the mechanical properties of AISI 1035.展开更多
Water-coupled charge blasting is a promising technique to efficiently break rock masses.In this study,numerical models of double boreholes with water-coupled charge are established using LS-DYNA and are calibrated by ...Water-coupled charge blasting is a promising technique to efficiently break rock masses.In this study,numerical models of double boreholes with water-coupled charge are established using LS-DYNA and are calibrated by the tests of rock masses subjected to explosion loads to examine its performance.The crack levels of rock mass induced by water-coupled charge blasting and air-coupled charge blasting are first compared.It is found that water-coupled charge blasting is more appropriate to fracture deep rock mass than air-coupled charge blasting.In addition,the effects of rock properties,water-coupled charge coefficients,and borehole connection angles on the performance of water-coupled charge blasting are investigated.The results show that rock properties and water-coupled charge coefficients can greatly influence the crack and fragmentation levels of rock mass induced by water-coupled charge blasting under uniform and non-uniform in-situ stresses.However,changing borehole-connection angles can only affect crack and fragmentation levels of rock mass under non-uniform in-situ stresses but barely affect those under uniform in-situ stresses.A formula is finally proposed by considering the above-mentioned factors to provide the design suggestion of water-coupled charge blasting to fracture rock mass with different in-situ stresses.展开更多
Gamma-aminobutyric acid(GABA),widely existing in different organisms,is rapidly accumulated in plants in response to environmental stresses.The main biosynthesis and degradation pathways of GABA constitute the GABA sh...Gamma-aminobutyric acid(GABA),widely existing in different organisms,is rapidly accumulated in plants in response to environmental stresses.The main biosynthesis and degradation pathways of GABA constitute the GABA shunt,which is tied to the tricarboxylic acid(TCA)cycle.GABA transaminase(GABA-T)and succinate semialdehyde dehydrogenase(SSADH)are two essential enzymes for the GABA degradation pathway.While there are abundant studies on GABA shunt in higher plants at the physiological and genetic levels,research on its role in microalgae remains limited.This study aimed at exploring the function of GABA-T and SSADH genes in Isochrysis zhanjiangensis,an important diet microalga,under different stresses.We cloned two GABA-T genes,IzGABA-T1 and IzGABA-T2,and one SSADH gene IzSSADH from Isochrysis zhanjiangensis and conducted heterologous expression experiments.The results showed that the overexpression of IzGABA-T1 or IzGABA-T2 enhanced the survival rates of yeast transformants under heat or NaCl stress,while the overexpression of IzSSADH improved yeast tolerance to NaCl stress but had no obvious effect on heat stress.Additionally,the results of quantitative real-time polymerase chain reaction(qPCR)showed that IzGABA-T1 transcription increased in the HT(salinity 25,35℃)and LS(salinity 15,25℃)groups.At 24 h,the IzGABA-T2 transcriptions increased in the HT,LS,and HS(salinity 35,25℃)groups,but their transcription levels decreased in all groups at 48 h.IzSSADH transcription increased in the LS group.These results suggest that IzGABA-T1,IzGABA-T2,and IzSSADH are associated with temperature and salinity stresses and possess a certain preference for different stresses.展开更多
Genes in the glycogen synthase kinase 3(GSK3)family are essential in regulating plant response to stressful conditions.This study employed bioinformatics to uncover the GSK3 gene family from the sunflower genome datab...Genes in the glycogen synthase kinase 3(GSK3)family are essential in regulating plant response to stressful conditions.This study employed bioinformatics to uncover the GSK3 gene family from the sunflower genome database.The expressions of GSK3 genes in different tissues and stress treatments,such as salt,drought,and cold,were assessed using transcriptome sequencing and quantitative real-time PCR(qRT-PCR).The study results revealed that the 12 GSK3 genes of sunflower,belonging to four classes(Classes I–IV),contained the GSK3 kinase domain and 11–13 exons.The majority of GSK3 genes were highly expressed in the leaf axil and flower,while their expression levels were relatively lower in the leaf.As a result of salt stress,six of the GSK3 genes(HaSK11,HaSK22,HaSK23,HaSK32,HaSK33,and HaSK41)displayed a notable increase in expression,while HaSK14 and HaSK21 experienced a significant decrease.With regard to drought stress,five of the GSK3 genes(HaSK11,HaSK13,HaSK21,HaSK22,and HaSK33)experienced a remarkable rise in expression.When exposed to cold stress,seven of the GSK3 genes(HaSK11,HaSK12,HaSK13,HaSK32,HaSK33,HaSK41,and HaSK42)showed a substantial increase,whereas HaSK21 and HaSK23 had a sharp decline.This research is of great importance in understanding the abiotic resistance mechanism of sunflowers and developing new varieties with improved stress resistance.展开更多
Reverse shoulder arthroplasty (RSA) is an effective treatment for rotator cuff tears. Despite its advantages, complications occur at a high rate. Complications requiring revision include a high rate of base plate fail...Reverse shoulder arthroplasty (RSA) is an effective treatment for rotator cuff tears. Despite its advantages, complications occur at a high rate. Complications requiring revision include a high rate of base plate failure, 38% of which are due to instability. The primary stability the base plate ensures is a crucial factor and, thus, is the subject of much debate in clinical studies and biomechanical research. This study is aimed to provide data that will contribute to the base plate’s pri-mary stability and glenoid longevity by clarifying the stresses at the scapular fossa and base plate interface associated with elevation after RSA. A 3D finite element model was created from the DICOM data for the scapulohumeral joint and SMR shoulder system. For loading conditions, 30 N was applied for each posi-tion with abduction angles of 0, 45, 90, and 135 degrees. A three-dimensional fi-nite element analysis was performed using the static implicit method with LS-DYNA. The von Mises stresses in the scapular fossa were found not to exceed the yield stress on the bone even after elevation to an abduction angle of 135 de-grees after RSA. It is rough to uniformly compare the yield stress and the von Mises stress, but it was inferred that the possibility of fracture is low unless a large external force is applied. A maximum von Mises stress showed 0 degrees of abduction, suggesting that the lowered position is in a more severe condition than the elevated position. If better improvement is desired, it may be necessary to devise ways to reduce the stress on the upper screw. .展开更多
Rolling path squeezes and rolling residual stresses of large diameter circular saw body for wood, generated by rolling pressure from 10 up to 120 bar were examined. X-ray diffraction, Barkhausen noise (BN) and Full Wi...Rolling path squeezes and rolling residual stresses of large diameter circular saw body for wood, generated by rolling pressure from 10 up to 120 bar were examined. X-ray diffraction, Barkhausen noise (BN) and Full Width of the peak at a Half Maximum (FWHM) (o) methods for evaluation of residual stresses were used. Dependencies of a tangential rolling residual stresses inside rolling paths upon rolling pressure p (bar) and rolling area A (mm2) were evaluated. The rolling pressure, as large as 60 bar, resulting in the rolling squeeze as high as 0.04 mm2, and, tangential residual compression stresses inside a rolling path, as large as ?TI = ?822 MPa, was considered to be the largest for the practical application.展开更多
Stress calculation formulae for a ring have been obtained by using Airy stress function of the plane strain field with the decomposition of the solutions for normal stresses of Airy biharmonic equation into two parts ...Stress calculation formulae for a ring have been obtained by using Airy stress function of the plane strain field with the decomposition of the solutions for normal stresses of Airy biharmonic equation into two parts when it is loaded under two opposite inside forces along a diameter. One part should fulfill a constraint condition about normal stress distribution along the circumference at an energy valley to do the minimum work. Other part is a stress residue constant. In order to verify these formulae and the computed results, the computed contour lines of equi-maximal shear stresses were plotted and quite compared with that of photo-elasticity test results. This constraint condition about normal stress distribution along circumference is confirmed by using Greens’ theorem. An additional compression exists along the circumference of the loaded ring, explaining the divorcement and displacement of singularity points at inner and outer boundaries.展开更多
In order to examine the effects of the decrease of future precipitation on the eco-physiological characteristics of sea buckthorn (Hippophae rhamnoides Linn.) in Huangfuchuan Watershed in Nei Mongol, a water gradient ...In order to examine the effects of the decrease of future precipitation on the eco-physiological characteristics of sea buckthorn (Hippophae rhamnoides Linn.) in Huangfuchuan Watershed in Nei Mongol, a water gradient experiment was conducted based on the four specially designed water supply levels, including normal precipitation, slight drought, drought and extreme drought. Results of ANOVE showed that different water gradients had a significant effect on (1) microhabitat factors, such as soil water content and soil temperature; (2) gas exchange, such as net photosynthetic rate, stomatal conductance and transpiration rate; (3) resource use efficiency; and (4) leaf water potential. Water use efficiency of H rhamnoides could increase under moderate water stress, i.e. drought condition, while its net photosynthetic rate and transpiration rate decreased. All kinds of eco-physiological characteristics proved H. rhamnoides seedlings under all water supplies were affected by water stress more or less and that mechanism of intrinsic physiological regulation in seedlings under the extreme drought conditions had the appearance of turbulence to a certain extent. Therefore, H rhamnoides seedlings in Huangfuchuan Watershed could not acclimate to extreme drought conditions.展开更多
基金supported by the National Key R&D Program of China(2022YFF1001800)the National Natural Science Foundation of China(32088102)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB0630103).
文摘Plantshave evolvedvariousmechanismsto interact withmicroorganisms,which help them acquire nutrients from the soil and enhance their tolerance to environmental stresses.One of the most widespread mutualistic interactions is arbuscular mycorrhizal(AM)symbiosis,which is formed by 80%-90%of terrestrial plants in association with AM fungi.In AM symbiosis,plants acquire mineral nutrients from the fungi in exchange for fatty acids and sugars that are produced during photosynthesis(Jiang et al.,2017).
基金The authors extend their gratitude to the Deanship of Scientific Research(DSR),King Faisal University,Saudi Arabia,for funding the publication of this work(Project number:KFU250560).
文摘A steady rise in the overall population is creating an overburden on crops due to their global demand.On the other hand,given the current climate change and population growth,agricultural practices established during the Green Revolution are no longer viable.Consequently,innovative practices are the prerequisite of the time struggle with the rising global food demand.The potential of nanotechnology to reduce the phytotoxic effects of these ecological restrictions has shown significant promise.Nanoparticles(NPs)typically enhance plant resilience to stressors by fortifying the physical barrier,optimizing photosynthesis,stimulating enzymatic activity for defense,elevating the concentration of stress-resistant compounds,and activating the expression of genes associated with defense mechanisms.In this review,we thoroughly cover the uptake and translocations of NPs crops and their potential valuable functions in enhancing plant growth and development at different growth stages.Additionally,we addressed how NPs improve plant resistance to biotic and abiotic stress.Generally,this review presents a thorough understanding of the significance of NPs in plants and their prospective value for plant antioxidant and crop development.
基金The SEU Innovation Capability Enhancement Plan for Doctoral Students(No.CXJH_SEU 24115)Marine Economic Development Project of Guangdong Province(No.GDNRC[2022]25).
文摘The study aimed to address the issue of elevated residual stress levels in dissimilar girth welds of cast steel joints.To achieve this,the hybrid welding technology,which yields high welding speeds while simultaneously reducing residual stresses,has been introduced.This study utilizes a numerical simulation method to investigate the temperature and residual stress field in the hybrid welding of G20Mn5 casting-Q355 low-alloy steel welded pipe.A com-parison of the findings of this study with those of other welding processes revealed the technological advantages of hybrid welding.The research outcomes show that due to geometric discontinuities and material differences,the temperature field of the joint exhibits uneven distribution characteristics,and the peak temperatures on the Q355 steel side exceeds those on the G20Mn5 steel side.An evident stress gra-dient is present in the residual stress field of the joint post-welding,with peak stress located at the weld root on the Q355 steel.Compared with arc welding,the hybrid welding leads to decreased residual stresses and deformation,with high stress outside the heat-affected zone diminishing rapidly.Furthermore,it significantly improves the welding efficiency.This study elucidates the distribution and underlying causes of thermal and residual stress fields in dissimilar girth welds.This serves as a foundation for the application of hybrid welding technology in welded cast steel joints.
文摘Wear is a prevalent issue across various industries. Spherical fused tungsten carbide (sFTC) reinforced nickel-aluminum bronze (NAB) matrix composite surface deposits have shown remarkable potential in mitigating wear by approximately 80%. However, the performance of these sFTC/NAB composite surface deposits is determined by their residual stress state, and the precise macroscopic and microscopic residual stresses within these composites have yet to be clearly established. To address this gap, we employed neutron diffraction to measure the residual stresses in the sFTC/NAB composite surface deposits and re-melted NAB samples produced via laser melt injection. Significant residual stresses were determined. The maximum tensile macro residual stress appears approximately 1-1.5 mm below the composite layer. Residual stresses accumulate with an increasing number of laser process tracks. The maximum tensile macro residual stress in the three-track samples reaches about 350 MPa. Preheating the base plate significantly reduces the levels of macroscopic residual stress. The WC phase displayed significant compressive thermal misfit residual stress magnitude, while the Cu matrix exhibited tensile thermal misfit residual stress. Preheating the base plate does not reduce microscopic thermal misfit residual stress levels. In addition, a finite element model was built to investigate temperature and residual stresses in the re-melted NAB samples. The predicted temperature history and residual stress agree with the experimental results.
基金funded by National Natural Science Foundation of China(grant no.32301870 to Chen Lin)Natural Science Foundation of Jiangsu Province(grant no.BK20230568 to Chen Lin)+3 种基金the Jiangsu Provincial Agricultural Science and Technology Independent Innovation Fund(grant no.CX(24)3124 to Chen Lin)Outstanding Ph.D.Programin Yangzhou(grant no.YZLYJFJH2022YXBS147 to Chen Lin)the General Project of Basic Scientific Research to colleges and universities in Jiangsu Province(grant no.22KJB210019 toChen Lin)the Priority Academic Program Development of Jiangsu Higher Education Institutions is greatly acknowledged.
文摘Glutathione S-transferases (GSTs) represent a large and diverse enzyme family ubiquitously distributed across the plant kingdom. These proteins catalyze the conjugation of glutathione (GSH) with electrophilic substrates in response to various stress conditions. Beyond their role in stress adaptation, certain GSTs are integral regulators of plant growth and development, contributing to a range of physiological processes. Most GST proteins exhibit dual enzymatic activities, functioning as both transferases and peroxidases, which enables their involvement in diverse cellular processes, including detoxification and stress responses. Recent advancements, particularly in X-ray crystallography, have enabled detailed structural analysis of GST proteins, significantly enhancing our understanding of their biological functions. This review offers a comprehensive overview of the classification and structural characteristics of GSTs in plants. It also highlights recent findings on their roles in plant growth and development, cell signaling, catalytic transport, and stress tolerance. Furthermore, key scientific challenges related to GSTs are discussed, focusing on their potential applications in agriculture. These insights aim to facilitate the screening of functional GST genes and support molecular breeding efforts across diverse crop species.
基金funded by the National Science Foundation of China(CN)(Nos.42090054,41922055,41931295)the Key Research and Development Program of Hubei Province of China(No.2020BCB079)。
文摘Landslides frequently occurred in Jurassic red strata in the Three Gorges Reservoir(TGR)region in China.The Jurassic strata consist of low mechanical strength and poor permeability of weak silty mudstone layer,which may cause slope instability during rainfall.In order to understand the strength behavior of Jurassic silty mudstone shear zone,the so-called Shizibao landslide located in Guojiaba Town,Zigui County,Three Gorges Reservoir(TGR)in China is selected as a case study.The shear strength of the silty mudstone shear zone is strongly influenced by both the water content and the normal stress.Therefore,a series of drained ring shear tests were carried out by varying the water contents(7%,12%,17%,and 20%,respectively)and normal stresses(200,300,400,and 500 kPa,respectively).The result revealed that the residual friction coefficient and residual friction angle were power function relationships with water content and normal stress.The peak cohesion of the silty mudstone slip zone increased with water content to a certain limit,above which the cohesion decreased.In contrast,the residual cohesion showed the opposite trend,indicating the cohesion recovery above a certain limit of water content.However,both the peak and residual friction angle of the silty mudstone slip zone were observed to decrease steadily with increased water content.Furthermore,the macroscopic morphological features of the shear surface showed that the sliding failure was developed under high normal stress at low water content,while discontinuous sliding surface and soil extrusion were occurred when the water content increased to a saturated degree.The localized liquefaction developed by excess pore water pressure reduced the frictional force within the shear zone.Finally,the combined effects of the slope excavation and precipitation ultimately lead to the failure of the silty mudstone slope;however,continuous rainfall is the main factor triggering sliding.
基金supported by the Fujian Province Seed Industry Innovation and Industrialization Project“Innovation and Industrialization Development of Precious Tree Seed Industries(Phoebe bornei)”(ZYCX-LY-202102)the Sub-project of National Key R&D Program“Phoebe bornei Efficient Cultivation Technology”(2016YFD0600603-2).
文摘Heat shock transcription factors(Hsfs)have important roles during plant growth and development and responses to abiotic stresses.The identification and func-tion of Hsf genes have been thoroughly studied in various herbaceous plant species,but not woody species,especially Phoebe bournei,an endangered,unique species in China.In this study,17 members of the Hsf gene family were identi-fied from P.bournei using bioinformatic methods.Phyloge-netic analysis indicated that PbHsf genes were grouped into three subfamilies:A,B,and C.Conserved motifs,three-dimensional structure,and physicochemical properties of the PbHsf proteins were also analyzed.The structure of the PbHsf genes varied in the number of exons and introns.Pre-diction of cis-acting elements in the promoter region indi-cated that PbHsf genes are likely involved in responses to plant hormones and stresses.A collinearity analysis dem-onstrated that expansions of the PbHsf gene family mainly take place via segmental duplication.The expression levels of PbHsf genes varied across different plant tissues.On the basis of the expression profiles of five representative PbHsf genes during heat,cold,salt,and drought stress,PbHsf pro-teins seem to have multiple functions depending on the type of abiotic stress.This systematic,genome-wide investigation of PbHsf genes in P.bournei and their expression patterns provides valuable insights and information for further func-tional dissection of Hsf proteins in this endangered,unique species.
基金National Natural Science Foundation of China(No.52005018)Beijing Natural Science Foundation(No.3232022)+1 种基金Fundamental Research Funds for the Central Universities(No.FRF-TP-22-035A1)National Defense Basic Scientific Research Program of China(No.JCKY2020601C004).
文摘Large 2219 Al-Cu alloy aerospace integral components suffer from long-term stress relaxation aging(SRA)due to complex temperature and stress loads during aging treatment/forming and service process,which makes it difficult to ensure their appropriate residual stress and excellent mechanical and service prop-erties.However,the research is limited to a thorough understanding of macroscopic and microscopic features and underlying mechanisms of the long-term SRA under multivariable aging conditions.There-fore,this study investigated macroscopic and microscopic features of long-term SRA under different tem-peratures(120 ℃ to 190 ℃),initial stress levels(100 MPa to 250 MPa)and durations(0 h to 50 h)through stress relaxation curves,metallographic traits,Vickers hardness,tensile performance,disloca-tions and phases of precipitation.On the basis of experimental outcomes,the comprehensive mecha-nisms beneath SRA were unraveled through dislocation theory,multiphase strengthening mechanisms and thermodynamics,where the interplays of stress relaxation behavior with age-hardening response were taken into consideration.The results showed elevations in the rate of stress reduction as the tem-perature and initial stress rose.At an initial stress greater than the yield stress of alloy,a marked in-crease in stress relaxation was found,and the mechanisms transform from the intragranular motion of dislocations and diffusion of grain boundaries to the intragranular and intergranular motion of disloca-tions and migration of grain boundaries.The stress reduction rate rose sharply when the temperature exceeded 175 ℃,and the dislocation movement mechanisms transform from gliding to climbing of dislo-cations.Stress relaxation is in nature progressive transformation of strain from elastic into a permanently inelastic state via the motion of dislocations,leading to the decrease of movable dislocations and the increase of immovable dislocations with more stable configurations.The age hardening is mainly deter-mined by precipitation strengthening,supplementarily by dislocation strengthening,and obvious stress orientation effect(SOE)of G.P.zones and θ"phases degenerates strengthening effect.The interplay be-tween stress relaxation behavior and age-hardening response influences the thermal-mechanical coupling SRA of 2219 Al-Cu alloy,which depends fundamentally on the motion of dislocations and their interplay with precipitated phases.This is a thermal activation process concerning the interplay between internal(age-hardening resistance)stress and external(initial)stress.The initial energy of elastic strain offers Gibbs free energy as the SRA driver,and a steady state of stress relaxation is attained with the lowest energy of elastic strain.These findings provide valuable insights into exploring innovative aging treat-ment/forming for optimizing residual stress,mechanical performance and service property in a synergistic manner.
基金The support of the S?o Paulo State University (UNESP)。
文摘In crop plants, various environmental stresses affect the balance of carbon, nitrogen, and phosphorus(C:N:P), leading to biochemical and physiological alterations and reductions in yield. Silicon(Si) is a beneficial element that alleviates plant stress. Most studies involving silicon have focused on physiological responses, such as improvements in photosynthetic processes, water use efficiency, and antioxidant defense systems. But recent research suggests that stressed plants facing either limited or excessive resources(water, light, nutrients, and toxic elements), strategically employ Si to maintain C:N:P homeostasis, thereby minimizing biomass losses. Understanding the role of Si in mitigating the impact of abiotic stresses on plants by regulating C:N:P homeostasis holds great potential for advancing sustainable agricultural practices in crop production. This review presents recent advances in characterizing the influence of environmental stresses on C:N:P homeostasis, as well as the role of Si in preserving C:N:P equilibrium and attenuating biological damage associated with abiotic stress. It underscores the beneficial effects of Si in sustaining C:N:P homeostasis and increasing yield via improved nutritional efficiency and stress mitigation.
文摘Boulder spacing in mountain rivers and near-wake flow zones within the boulder array is very useful for fish habitat and growth of aquatic organisms.The present study aims to investigate how the boulder array and spacing influence the near-bed flow structures in a gravel-bed stream.Boulders are staggered over a gravel-bed stream with three different inter-boulder spacing namely(a)large(b)medium and(c)small spacing.An acoustic Doppler velocimeter was used for flow measurements in a rectangular channel and the results were compared with those acquired from numerical simulation.The time-averaged velocity profiles at the near-wake flow zones of boulders experience maximum flow retardation which is an outcome of the boulder-induced form roughness.The ratio of velocity differences associated to form and skin roughness and its positive magnitude reveals the dominance of form roughness closest to the boulders.Form roughness computed is 1.75 to 2 times higher than the skin roughness at the near-wake flow region.In particular,the collective immobile boulders placed at different inter-boulder spacings developed high and low bed shear stresses closest to the boulders.The low bed shear stresses characterised by a secondary peak developed at the trough location of the boulders is attributed to the skin shear stress.Further,the spatial averaging of time-averaged flow quantities gives additional impetus to present an improved illustration of fluid shear stresses.The formation of form-induced shear stress is estimated to be 17%to 23%of doubleaveraged Reynolds shear stress and partially compensates for the damping of time-averaged Reynolds shear stress in the interfacial sub-layer.The quadrant analysis of spatial velocity fluctuations depicts that the form-induced shear stresses are dominant in the interfacial sub-layer and have no significance above the gravel-bed surface.
文摘Purpose-The experiments of this study investigated the effect of the subgrade degree of saturation on the value of the stresses generated on the surface and the middle(vertical and lateral stresses).The objectives of this study can be identified by studying the effect of subgrade layer degree of saturation variation,load amplitude and load frequency on the transmitted stresses through the ballast layer to the subgrade layer and the stress distribution inside it and investigating the excess pore water pressure development in the clay layer in the case of a fully saturated subgrade layer and the change in matric suction in the case of an unsaturated subgrade layer.Design/methodology/approach-Thirty-six laboratory experiments were conducted using approximately half-scale replicas of real railways,with an iron box measuring 1.5×1.031.0 m.Inside the box,a 0.5 m thick layer of clay soil representing the base layer was built.Above it is a 0.2 m thick ballast layer made of crushed stone,and on top of that is a 0.8 m long rail line supported by three 0.9 m(0.1×0.1 m)slipper beams.The subgrade layer has been built at the following various saturation levels:100,80,70 and 60%.Experiments were conducted with various frequencies of 1,2 and 4 Hz with load amplitudes of 15,25 and 35 kN.Findings-The results of the study demonstrated that as the subgrade degree of saturation decreased from 100 to 60%,the ratio of stress in the lateral direction to stress in the vertical direction generated in the middle of the subgrade layer decreased as well.On average,this ratio changed from approximately 0.75 to approximately 0.65.Originality/value-The study discovered that as the test proceeded and the number of cycles increased,the value of negative water pressure(matric suction)in the case of unsaturated subgrade soils declined.The frequency of loads had no bearing on the ratio of decline in matric suction values,which was greater under a larger load amplitude than a lower one.As the test progressed(as the number of cycles increased),the matric suction dropped.For larger load amplitudes,there is a greater shift in matric suction.The change in matric suction is greater at higher saturation levels than it is at lower saturation levels.Furthermore,it is seen that the load frequency value has no bearing on how the matric suction changes.For all load frequencies and subgrade layer saturation levels,the track panel settlement rises with the load amplitude.Higher load frequency and saturation levels have a greater impact.
基金supported by the National Natural Science Foundation of China(Nos.T2288101,12172034,U20A20390,11827803,12202036)the Beijing Municipal Natural Science Foundation(No.7212205)+1 种基金the 111 Project(No.B13003)the Fundamental Research Funds for the Central Universities.
文摘High-purity(HP)magnesium(Mg)has emerged as a promising biomaterial for supporting functional bone tissue.Our previous study found that mechanical stresses and the surrounding fibrotic tissue(subcuta-neous)both play crucial roles in the degradation of HP Mg.However,due to challenges in the degradation and regeneration process in vivo,it remains unclear how stress affects HP Mg degradation in bone en-vironments,limiting its further application.In this study,novel loading devices were designed and the effects of tensile and compressive stresses on HP Mg degradation in vivo and in vitro bone environments were quantitatively analyzed.In addition,bone osteointegration around HP Mg was explored preliminar-ily.Tensile stress increases the degradation rate of HP Mg in vivo and in vitro.HP Mg degradation in vivo is more sensitive to stress factors than in vitro,but the sensitivity decreases with corrosion time.The volume loss rate of HP Mg is multilinear with the applied stress and degradation time.The volume of bone tissue surrounding HP Mg is larger in the no-stress group compared to the stressed groups,which is more pronounced with increasing implantation time.These results provide valuable insights for optimiz-ing the design of HP Mg-based implants considering load conditions.This will help to achieve a balance between the degradation rate of the implant and the regeneration rate of the surrounding bone.
基金Kut Technical Institute for their funding supports。
文摘The present study establishes a new estimation model using an artificial neural network(ANN) to predict the mechanical properties of the AISI 1035 alloy.The experiments were designed based on the L16 orthogonal array of the Taguchi method.A proposed numerical model for predicting the correlation of mechanical properties was supplemented with experimental data.The quenching process was conducted using a cooling medium called “nanofluids”.Nanoparticles were dissolved in a liquid phase at various concentrations(0.5%,1%,2.5%,and 5% vf) to prepare the nanofluids.Experimental investigations were done to assess the impact of temperature,base fluid,volume fraction,and soaking time on the mechanical properties.The outcomes showed that all conditions led to a noticeable improvement in the alloy's hardness which reached 100%,the grain size was refined about 80%,and unwanted residual stresses were removed from 50 to 150 MPa.Adding 5% of CuO nanoparticles to oil led to the best grain size refinement,while adding 2.5% of Al_(2)O_(3) nanoparticles to engine oil resulted in the greatest compressive residual stress.The experimental variables were used as the input data for the established numerical ANN model,and the mechanical properties were the output.Upwards of 99% of the training network's correlations seemed to be positive.The estimated result,nevertheless,matched the experimental dataset exactly.Thus,the ANN model is an effective tool for reflecting the effects of quenching conditions on the mechanical properties of AISI 1035.
基金Projects(52334003,52104111,52274249)supported by the National Natural Science Foundation of ChinaProject(2022YFC2903901)supported by the National Key R&D Project of ChinaProject(2024JJ4064)supported by the Natural Science Foundation of Hunan Province,China。
文摘Water-coupled charge blasting is a promising technique to efficiently break rock masses.In this study,numerical models of double boreholes with water-coupled charge are established using LS-DYNA and are calibrated by the tests of rock masses subjected to explosion loads to examine its performance.The crack levels of rock mass induced by water-coupled charge blasting and air-coupled charge blasting are first compared.It is found that water-coupled charge blasting is more appropriate to fracture deep rock mass than air-coupled charge blasting.In addition,the effects of rock properties,water-coupled charge coefficients,and borehole connection angles on the performance of water-coupled charge blasting are investigated.The results show that rock properties and water-coupled charge coefficients can greatly influence the crack and fragmentation levels of rock mass induced by water-coupled charge blasting under uniform and non-uniform in-situ stresses.However,changing borehole-connection angles can only affect crack and fragmentation levels of rock mass under non-uniform in-situ stresses but barely affect those under uniform in-situ stresses.A formula is finally proposed by considering the above-mentioned factors to provide the design suggestion of water-coupled charge blasting to fracture rock mass with different in-situ stresses.
基金supported by the Zhejiang Provincial Natural Science Foundation of China(No.LY22C190001)the Natural Science Foundation of Ningbo Government(No.2021J114)+3 种基金the Ningbo Science and Technology Research Projects,China(No.2019B10006)the Zhejiang Provincial Department of Education Scientific Research Project(No.Y202249030)the Earmarked Fund for CARS-49partly sponsored by K.C.Wong Magna Fund in Ningbo University.
文摘Gamma-aminobutyric acid(GABA),widely existing in different organisms,is rapidly accumulated in plants in response to environmental stresses.The main biosynthesis and degradation pathways of GABA constitute the GABA shunt,which is tied to the tricarboxylic acid(TCA)cycle.GABA transaminase(GABA-T)and succinate semialdehyde dehydrogenase(SSADH)are two essential enzymes for the GABA degradation pathway.While there are abundant studies on GABA shunt in higher plants at the physiological and genetic levels,research on its role in microalgae remains limited.This study aimed at exploring the function of GABA-T and SSADH genes in Isochrysis zhanjiangensis,an important diet microalga,under different stresses.We cloned two GABA-T genes,IzGABA-T1 and IzGABA-T2,and one SSADH gene IzSSADH from Isochrysis zhanjiangensis and conducted heterologous expression experiments.The results showed that the overexpression of IzGABA-T1 or IzGABA-T2 enhanced the survival rates of yeast transformants under heat or NaCl stress,while the overexpression of IzSSADH improved yeast tolerance to NaCl stress but had no obvious effect on heat stress.Additionally,the results of quantitative real-time polymerase chain reaction(qPCR)showed that IzGABA-T1 transcription increased in the HT(salinity 25,35℃)and LS(salinity 15,25℃)groups.At 24 h,the IzGABA-T2 transcriptions increased in the HT,LS,and HS(salinity 35,25℃)groups,but their transcription levels decreased in all groups at 48 h.IzSSADH transcription increased in the LS group.These results suggest that IzGABA-T1,IzGABA-T2,and IzSSADH are associated with temperature and salinity stresses and possess a certain preference for different stresses.
基金financed by the Anhui Provincial Central Leading Local Science and Technology Development Special Fund Project(202007d06020021)Project of Suzhou Science and Technology Bureau(2021143).
文摘Genes in the glycogen synthase kinase 3(GSK3)family are essential in regulating plant response to stressful conditions.This study employed bioinformatics to uncover the GSK3 gene family from the sunflower genome database.The expressions of GSK3 genes in different tissues and stress treatments,such as salt,drought,and cold,were assessed using transcriptome sequencing and quantitative real-time PCR(qRT-PCR).The study results revealed that the 12 GSK3 genes of sunflower,belonging to four classes(Classes I–IV),contained the GSK3 kinase domain and 11–13 exons.The majority of GSK3 genes were highly expressed in the leaf axil and flower,while their expression levels were relatively lower in the leaf.As a result of salt stress,six of the GSK3 genes(HaSK11,HaSK22,HaSK23,HaSK32,HaSK33,and HaSK41)displayed a notable increase in expression,while HaSK14 and HaSK21 experienced a significant decrease.With regard to drought stress,five of the GSK3 genes(HaSK11,HaSK13,HaSK21,HaSK22,and HaSK33)experienced a remarkable rise in expression.When exposed to cold stress,seven of the GSK3 genes(HaSK11,HaSK12,HaSK13,HaSK32,HaSK33,HaSK41,and HaSK42)showed a substantial increase,whereas HaSK21 and HaSK23 had a sharp decline.This research is of great importance in understanding the abiotic resistance mechanism of sunflowers and developing new varieties with improved stress resistance.
文摘Reverse shoulder arthroplasty (RSA) is an effective treatment for rotator cuff tears. Despite its advantages, complications occur at a high rate. Complications requiring revision include a high rate of base plate failure, 38% of which are due to instability. The primary stability the base plate ensures is a crucial factor and, thus, is the subject of much debate in clinical studies and biomechanical research. This study is aimed to provide data that will contribute to the base plate’s pri-mary stability and glenoid longevity by clarifying the stresses at the scapular fossa and base plate interface associated with elevation after RSA. A 3D finite element model was created from the DICOM data for the scapulohumeral joint and SMR shoulder system. For loading conditions, 30 N was applied for each posi-tion with abduction angles of 0, 45, 90, and 135 degrees. A three-dimensional fi-nite element analysis was performed using the static implicit method with LS-DYNA. The von Mises stresses in the scapular fossa were found not to exceed the yield stress on the bone even after elevation to an abduction angle of 135 de-grees after RSA. It is rough to uniformly compare the yield stress and the von Mises stress, but it was inferred that the possibility of fracture is low unless a large external force is applied. A maximum von Mises stress showed 0 degrees of abduction, suggesting that the lowered position is in a more severe condition than the elevated position. If better improvement is desired, it may be necessary to devise ways to reduce the stress on the upper screw. .
基金support of the Poznan Networking&Supercomputing Center(PCSS)calculation grant
文摘Rolling path squeezes and rolling residual stresses of large diameter circular saw body for wood, generated by rolling pressure from 10 up to 120 bar were examined. X-ray diffraction, Barkhausen noise (BN) and Full Width of the peak at a Half Maximum (FWHM) (o) methods for evaluation of residual stresses were used. Dependencies of a tangential rolling residual stresses inside rolling paths upon rolling pressure p (bar) and rolling area A (mm2) were evaluated. The rolling pressure, as large as 60 bar, resulting in the rolling squeeze as high as 0.04 mm2, and, tangential residual compression stresses inside a rolling path, as large as ?TI = ?822 MPa, was considered to be the largest for the practical application.
文摘Stress calculation formulae for a ring have been obtained by using Airy stress function of the plane strain field with the decomposition of the solutions for normal stresses of Airy biharmonic equation into two parts when it is loaded under two opposite inside forces along a diameter. One part should fulfill a constraint condition about normal stress distribution along the circumference at an energy valley to do the minimum work. Other part is a stress residue constant. In order to verify these formulae and the computed results, the computed contour lines of equi-maximal shear stresses were plotted and quite compared with that of photo-elasticity test results. This constraint condition about normal stress distribution along circumference is confirmed by using Greens’ theorem. An additional compression exists along the circumference of the loaded ring, explaining the divorcement and displacement of singularity points at inner and outer boundaries.
文摘In order to examine the effects of the decrease of future precipitation on the eco-physiological characteristics of sea buckthorn (Hippophae rhamnoides Linn.) in Huangfuchuan Watershed in Nei Mongol, a water gradient experiment was conducted based on the four specially designed water supply levels, including normal precipitation, slight drought, drought and extreme drought. Results of ANOVE showed that different water gradients had a significant effect on (1) microhabitat factors, such as soil water content and soil temperature; (2) gas exchange, such as net photosynthetic rate, stomatal conductance and transpiration rate; (3) resource use efficiency; and (4) leaf water potential. Water use efficiency of H rhamnoides could increase under moderate water stress, i.e. drought condition, while its net photosynthetic rate and transpiration rate decreased. All kinds of eco-physiological characteristics proved H. rhamnoides seedlings under all water supplies were affected by water stress more or less and that mechanism of intrinsic physiological regulation in seedlings under the extreme drought conditions had the appearance of turbulence to a certain extent. Therefore, H rhamnoides seedlings in Huangfuchuan Watershed could not acclimate to extreme drought conditions.
