目的采用酵母双杂交法,从小鼠胚胎干细胞cDNA文库中筛选与Stra8(stimulated by retinoic acid8,Stra8)相互作用的蛋白。通过对相互作用蛋白的筛选和研究,探讨Stra8在胚胎干细胞分化中的作用机制。方法以pGBKT7-Stra8为诱饵质粒,从小鼠...目的采用酵母双杂交法,从小鼠胚胎干细胞cDNA文库中筛选与Stra8(stimulated by retinoic acid8,Stra8)相互作用的蛋白。通过对相互作用蛋白的筛选和研究,探讨Stra8在胚胎干细胞分化中的作用机制。方法以pGBKT7-Stra8为诱饵质粒,从小鼠胚胎干细胞cDNA文库中筛选出与Stra8相互作用的阳性克隆,对验证后的阳性克隆进行测序及生物信息学分析,进一步采用直接酵母双杂交进行相互作用的验证。结果经过筛选、测序、同源性分析及直接酵母双杂交验证,发现了Setd6及DEK 2种与Stra8相互作用的蛋白。结论应用酵母双杂交系统,筛选得到了与Stra8相互作用的蛋白Setd6及DEK,它们可能与Stra8一起调控胚胎干细胞的增殖与分化,对Stra8相互作用蛋白的筛选有助于揭示其在胚胎干细胞中的作用机制。展开更多
Stra8(stimulated by retinoic acid gene8)是一种生殖细胞特异性表达并受视黄酸诱导的基因,它可有效促进生殖细胞启动减数分裂。Stra8是减数分裂起始的调节蛋白,参与了雌性胚胎期和雄性出生后生殖细胞的减数分裂起始过程。胚胎期睾丸...Stra8(stimulated by retinoic acid gene8)是一种生殖细胞特异性表达并受视黄酸诱导的基因,它可有效促进生殖细胞启动减数分裂。Stra8是减数分裂起始的调节蛋白,参与了雌性胚胎期和雄性出生后生殖细胞的减数分裂起始过程。胚胎期睾丸减数分裂起始受阻是由于视黄酸降解酶CYP26B1和发育相关调节蛋白Nanos2抑制Stra8表达的结果。Stra8与生殖细胞减数分裂起始的研究有助于人们对精子生成和卵子发育的深入理解。展开更多
With the continuous transformation and upgrading of China's economic development, "mass entrepreneurship and innovation" has become the consensus of social development. In this context, how to do a good ...With the continuous transformation and upgrading of China's economic development, "mass entrepreneurship and innovation" has become the consensus of social development. In this context, how to do a good job in the education and teaching of the discipline of public administration, so that the education concept and teaching mode of the discipline can meet the strategic demands of the development of the new era and the social needs of talent training, which has become a major issue faced by colleges and universities and teachers in the process of education and teaching. Therefore, through the analysis of the current training mode of public administration talents in colleges and universities, this paper explores the specific strategies for the establishment and optimization of the practical teaching system of public administration talents under the background of innovation and entrepreneurship.展开更多
文摘目的采用酵母双杂交法,从小鼠胚胎干细胞cDNA文库中筛选与Stra8(stimulated by retinoic acid8,Stra8)相互作用的蛋白。通过对相互作用蛋白的筛选和研究,探讨Stra8在胚胎干细胞分化中的作用机制。方法以pGBKT7-Stra8为诱饵质粒,从小鼠胚胎干细胞cDNA文库中筛选出与Stra8相互作用的阳性克隆,对验证后的阳性克隆进行测序及生物信息学分析,进一步采用直接酵母双杂交进行相互作用的验证。结果经过筛选、测序、同源性分析及直接酵母双杂交验证,发现了Setd6及DEK 2种与Stra8相互作用的蛋白。结论应用酵母双杂交系统,筛选得到了与Stra8相互作用的蛋白Setd6及DEK,它们可能与Stra8一起调控胚胎干细胞的增殖与分化,对Stra8相互作用蛋白的筛选有助于揭示其在胚胎干细胞中的作用机制。
文摘Stra8(stimulated by retinoic acid gene8)是一种生殖细胞特异性表达并受视黄酸诱导的基因,它可有效促进生殖细胞启动减数分裂。Stra8是减数分裂起始的调节蛋白,参与了雌性胚胎期和雄性出生后生殖细胞的减数分裂起始过程。胚胎期睾丸减数分裂起始受阻是由于视黄酸降解酶CYP26B1和发育相关调节蛋白Nanos2抑制Stra8表达的结果。Stra8与生殖细胞减数分裂起始的研究有助于人们对精子生成和卵子发育的深入理解。
文摘With the continuous transformation and upgrading of China's economic development, "mass entrepreneurship and innovation" has become the consensus of social development. In this context, how to do a good job in the education and teaching of the discipline of public administration, so that the education concept and teaching mode of the discipline can meet the strategic demands of the development of the new era and the social needs of talent training, which has become a major issue faced by colleges and universities and teachers in the process of education and teaching. Therefore, through the analysis of the current training mode of public administration talents in colleges and universities, this paper explores the specific strategies for the establishment and optimization of the practical teaching system of public administration talents under the background of innovation and entrepreneurship.
