The influence of low-vacuum helium cold plasma treatment on the rooting percentage,root growth and physiochemical properties of zoysiagrass stolon cuttings was studied.Zoysiagrass stolon cuttings were pre-treated with...The influence of low-vacuum helium cold plasma treatment on the rooting percentage,root growth and physiochemical properties of zoysiagrass stolon cuttings was studied.Zoysiagrass stolon cuttings were pre-treated with 0,100,200,300 and 400 W of cold plasma for 15 s.The cold plasma positively stimulated rooting and improved the root growth of the zoysiagrass stolon cuttings,and the 300 W treatment produced the best effect.The rooting percentage and root growth parameters,including the root length,total root surface area,total root volume,average root diameter,and root dry weight,significantly improved in response to the cold plasma treatment.In addition,the water uptake and relative conductivity of the stolon cuttings increased significantly in response to the cold plasma treatment.The results revealed that cold plasma-stimulated rooting and root growth appear to be a consequence of the improvement in permeability and water absorbing capacity of zoysiagrass stolon cuttings.The results of the present study will provide inspiration and support for the application of cold plasma in the vegetative propagation of plants.展开更多
The strawberry axillary meristem can develop into a branch crown(a flowering shoot)or a stolon(a horizontal stem that produces daughter plants),with gibberellin promoting stolon fate.Despite its importance for plant a...The strawberry axillary meristem can develop into a branch crown(a flowering shoot)or a stolon(a horizontal stem that produces daughter plants),with gibberellin promoting stolon fate.Despite its importance for plant architecture,asexual reproduction,and perennial growth,the regulatory mechanism governing this fate decision remains poorly understood.In this study,we found that the juvenile-to-adult transition of the axillary meristem is marked by induction of GA20ox4 expression and the onset of stolon formation.RNA sequencing of staged meristems identified the zinc-finger protein ZFP6,which is strongly coexpressed with GA20ox4.CRISPR-mediated knockout of ZFP6 abolished GA20ox4 expression and eliminated stolon formation,a phenotype rescued by exogenous gibberellin,demonstrating that ZFP6 acts to activate gibberellin biosynthesis and promote stolon formation.Genetic analysis of mutants in the redlight receptor PhyB,together with mutants in gibberellin biosynthesis and signaling,further revealed that red light and PhyB promote axillary meristem maturation,a previously unrecognized developmental stage that precedes fate determination.Taken together,these findings illuminate how developmental stage and environmental cues converge to regulate axillary meristem maturation and fate determination,and identify a stage-specific regulator controlling the switch to stolon formation.展开更多
基金supported by the Jiangsu Provincial Public Institutions Program for Research Conditions and Building Capacity(No.BM2015019)the Foundation of Jiangsu Key Laboratory for the Research and Utilization of Plant Resources(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,No.JSPKLB201606)National Natural Science Foundation of China(No.31572155)
文摘The influence of low-vacuum helium cold plasma treatment on the rooting percentage,root growth and physiochemical properties of zoysiagrass stolon cuttings was studied.Zoysiagrass stolon cuttings were pre-treated with 0,100,200,300 and 400 W of cold plasma for 15 s.The cold plasma positively stimulated rooting and improved the root growth of the zoysiagrass stolon cuttings,and the 300 W treatment produced the best effect.The rooting percentage and root growth parameters,including the root length,total root surface area,total root volume,average root diameter,and root dry weight,significantly improved in response to the cold plasma treatment.In addition,the water uptake and relative conductivity of the stolon cuttings increased significantly in response to the cold plasma treatment.The results revealed that cold plasma-stimulated rooting and root growth appear to be a consequence of the improvement in permeability and water absorbing capacity of zoysiagrass stolon cuttings.The results of the present study will provide inspiration and support for the application of cold plasma in the vegetative propagation of plants.
基金supported by the National Science Foundation Award(IOS1935169)to Z.L.
文摘The strawberry axillary meristem can develop into a branch crown(a flowering shoot)or a stolon(a horizontal stem that produces daughter plants),with gibberellin promoting stolon fate.Despite its importance for plant architecture,asexual reproduction,and perennial growth,the regulatory mechanism governing this fate decision remains poorly understood.In this study,we found that the juvenile-to-adult transition of the axillary meristem is marked by induction of GA20ox4 expression and the onset of stolon formation.RNA sequencing of staged meristems identified the zinc-finger protein ZFP6,which is strongly coexpressed with GA20ox4.CRISPR-mediated knockout of ZFP6 abolished GA20ox4 expression and eliminated stolon formation,a phenotype rescued by exogenous gibberellin,demonstrating that ZFP6 acts to activate gibberellin biosynthesis and promote stolon formation.Genetic analysis of mutants in the redlight receptor PhyB,together with mutants in gibberellin biosynthesis and signaling,further revealed that red light and PhyB promote axillary meristem maturation,a previously unrecognized developmental stage that precedes fate determination.Taken together,these findings illuminate how developmental stage and environmental cues converge to regulate axillary meristem maturation and fate determination,and identify a stage-specific regulator controlling the switch to stolon formation.
