[Objectives] This study was conducted to establish the genetic marker Bi-PASA of STCH gene in Jinfen white pig and New Shanxi black pig. [Methods] The polymorphism of STCH gene was detected in Jinfen white pig and New...[Objectives] This study was conducted to establish the genetic marker Bi-PASA of STCH gene in Jinfen white pig and New Shanxi black pig. [Methods] The polymorphism of STCH gene was detected in Jinfen white pig and New Shanxi black pig by bidirectional PCR amplification of specific alleles. [Results] There were two alleles(A, B) and three genotypes(AA, AB and BB) in Jinfen white pig and New Shanxi black pig. The PopGene32 analysis showed that the polymorphic site of STCH gene in Jinfen white pig accorded with the Hardy-Weinberg equilibrium(P>0.05), while that of new Shanxi black pig did not meet the Hardy-Weinberg equilibrium(P<0.01). The results of chi-square test showed that the polymorphic site of STCH gene was significantly different in genotype distribution between the two pig breeds(P<0.01). [Conclusions] The establishment of the genetic marker Bi-PASA of STCH gene in Jinfen white pig and new Shanxi black pig could provide basic biological data for improving disease resistance of pigs and establishing disease-resistant populations.展开更多
基金Supported by Doctoral Scientific Research Staring Foundation(YQ-20190010)Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(2019L0878)
文摘[Objectives] This study was conducted to establish the genetic marker Bi-PASA of STCH gene in Jinfen white pig and New Shanxi black pig. [Methods] The polymorphism of STCH gene was detected in Jinfen white pig and New Shanxi black pig by bidirectional PCR amplification of specific alleles. [Results] There were two alleles(A, B) and three genotypes(AA, AB and BB) in Jinfen white pig and New Shanxi black pig. The PopGene32 analysis showed that the polymorphic site of STCH gene in Jinfen white pig accorded with the Hardy-Weinberg equilibrium(P>0.05), while that of new Shanxi black pig did not meet the Hardy-Weinberg equilibrium(P<0.01). The results of chi-square test showed that the polymorphic site of STCH gene was significantly different in genotype distribution between the two pig breeds(P<0.01). [Conclusions] The establishment of the genetic marker Bi-PASA of STCH gene in Jinfen white pig and new Shanxi black pig could provide basic biological data for improving disease resistance of pigs and establishing disease-resistant populations.
