目的找出与间变胶质瘤癫痫起病相关的基因改变。方法从The Cancer Genome Atlas(TCGA)网站下载胶质瘤全基因组测序数据库,其中包含间变胶质瘤243例,其中119例是以癫痫症状起病,其他124例是以非癫痫症状起病,运用非配对t检验来筛选癫痫...目的找出与间变胶质瘤癫痫起病相关的基因改变。方法从The Cancer Genome Atlas(TCGA)网站下载胶质瘤全基因组测序数据库,其中包含间变胶质瘤243例,其中119例是以癫痫症状起病,其他124例是以非癫痫症状起病,运用非配对t检验来筛选癫痫起病组和非癫痫起病组的差异基因,中国脑胶质瘤基因组图谱计划(CGGA)作为验证,Kaplan-Meier曲线被用来检测预后的分布结果。结果在TCGA中,本研究发现在间变胶质瘤中,以癫痫起病的患者预后要好于非癫痫起病的患者(P=0.04)。在癫痫起病组和非癫痫起病组,902个基因表达量有差别,将902个基因带入DAVID网站(https://david.ncifcrf.gov)进行分析,调控多种细胞组织生长通路在这两组差别最大,主要是7个基因(STAT5B、AFG3L2、FTO、APP、GDF5、MBD5、PIK3CA),在中国脑胶质瘤基因组图谱(CGGA)进行验证,只有STAT5B是有意义的,但是STAT5B不能够预测患者预后。结论间变胶质瘤中,有术前癫痫的患者的生存期明显好于无术前癫痫的患者。STAT5B高表达的患者不容易发生术前癫痫。STAT5B在肿瘤中表达量决定患者是否以癫痫起病。展开更多
Background: The late detection of endometrial carcinoma (EC) at an advanced stage often results in a poorpatient prognosis. It is hence important to identify reliable biomarkers to facilitate early detection of EC. Si...Background: The late detection of endometrial carcinoma (EC) at an advanced stage often results in a poorpatient prognosis. It is hence important to identify reliable biomarkers to facilitate early detection of EC. Signaltransducer and activator of transcription (STAT) family members play an important role in several tumors, however,their impact on EC development and progression remains unclear. Methods: Machine learning methods were used toinvestigate the importance of STAT5B in EC. Results: Hence, we explored the UALCAN data mining platform andfound that while STAT1 and STAT2 were upregulated, STAT5A, STAT5B, and STAT6 were downregulated in EC.This high expression of STAT5B and STAT6 predicted favorable clinical outcomes, whereas the increased expressionof STAT1 and STAT2 predicted poor clinical outcomes. Subsequent pathway enrichment analysis revealed that theSTAT family was mainly involved in apoptosis pathway activation, cell cycle disruption, and epithelial–mesenchymaltransition. Drug sensitivity analysis demonstrated that STAT5A/5B expression was negatively correlated with drugresistance in EC. Further, the expression of STAT5B mRNA and protein was correlated with severalclinicopathological characteristics. Tumor Immune Estimation Resource (TIMER) analysis revealed that STAT5Bexpression was positively correlated with the abundance of infiltrating CD8+ T cells and neutrophils while its copynumber variation was associated with the overall immune cell infiltration. The data on the correlations betweenSTAT5B expression and related genes in uterine corpus endometrial carcinoma (UCEC) in cBio Cancer Portalshowed the closest correlation of STAT5B expression with that of KIAA0753 (also known as moonraker and OFIP),followed by COL27A1 in EC. Pathway enrichment analysis further showed that STAT5B-related genes were involvedin the mitogen-activated protein kinase (MAPK) and Ras signaling pathways. Conclusion: Collectively, our findingsprovided new insights into the role of the STAT family in EC. It also highlighted new targets for future research ondiagnostic and prognostic markers and STAT5B as a novel marker for drug sensitivity screening.展开更多
文摘目的找出与间变胶质瘤癫痫起病相关的基因改变。方法从The Cancer Genome Atlas(TCGA)网站下载胶质瘤全基因组测序数据库,其中包含间变胶质瘤243例,其中119例是以癫痫症状起病,其他124例是以非癫痫症状起病,运用非配对t检验来筛选癫痫起病组和非癫痫起病组的差异基因,中国脑胶质瘤基因组图谱计划(CGGA)作为验证,Kaplan-Meier曲线被用来检测预后的分布结果。结果在TCGA中,本研究发现在间变胶质瘤中,以癫痫起病的患者预后要好于非癫痫起病的患者(P=0.04)。在癫痫起病组和非癫痫起病组,902个基因表达量有差别,将902个基因带入DAVID网站(https://david.ncifcrf.gov)进行分析,调控多种细胞组织生长通路在这两组差别最大,主要是7个基因(STAT5B、AFG3L2、FTO、APP、GDF5、MBD5、PIK3CA),在中国脑胶质瘤基因组图谱(CGGA)进行验证,只有STAT5B是有意义的,但是STAT5B不能够预测患者预后。结论间变胶质瘤中,有术前癫痫的患者的生存期明显好于无术前癫痫的患者。STAT5B高表达的患者不容易发生术前癫痫。STAT5B在肿瘤中表达量决定患者是否以癫痫起病。
文摘Background: The late detection of endometrial carcinoma (EC) at an advanced stage often results in a poorpatient prognosis. It is hence important to identify reliable biomarkers to facilitate early detection of EC. Signaltransducer and activator of transcription (STAT) family members play an important role in several tumors, however,their impact on EC development and progression remains unclear. Methods: Machine learning methods were used toinvestigate the importance of STAT5B in EC. Results: Hence, we explored the UALCAN data mining platform andfound that while STAT1 and STAT2 were upregulated, STAT5A, STAT5B, and STAT6 were downregulated in EC.This high expression of STAT5B and STAT6 predicted favorable clinical outcomes, whereas the increased expressionof STAT1 and STAT2 predicted poor clinical outcomes. Subsequent pathway enrichment analysis revealed that theSTAT family was mainly involved in apoptosis pathway activation, cell cycle disruption, and epithelial–mesenchymaltransition. Drug sensitivity analysis demonstrated that STAT5A/5B expression was negatively correlated with drugresistance in EC. Further, the expression of STAT5B mRNA and protein was correlated with severalclinicopathological characteristics. Tumor Immune Estimation Resource (TIMER) analysis revealed that STAT5Bexpression was positively correlated with the abundance of infiltrating CD8+ T cells and neutrophils while its copynumber variation was associated with the overall immune cell infiltration. The data on the correlations betweenSTAT5B expression and related genes in uterine corpus endometrial carcinoma (UCEC) in cBio Cancer Portalshowed the closest correlation of STAT5B expression with that of KIAA0753 (also known as moonraker and OFIP),followed by COL27A1 in EC. Pathway enrichment analysis further showed that STAT5B-related genes were involvedin the mitogen-activated protein kinase (MAPK) and Ras signaling pathways. Conclusion: Collectively, our findingsprovided new insights into the role of the STAT family in EC. It also highlighted new targets for future research ondiagnostic and prognostic markers and STAT5B as a novel marker for drug sensitivity screening.
