In the present study, the interaction of human p100 protein with signal transducer and activator of transcription-6 (STAT-6) was investigated. It was proved that the staphylococcal nuclease (SN)-like and tudor (TD) do...In the present study, the interaction of human p100 protein with signal transducer and activator of transcription-6 (STAT-6) was investigated. It was proved that the staphylococcal nuclease (SN)-like and tudor (TD) domains containing in p100 protein acting as a adaptor to recruit STAT-6 to the basal transcription machinery, enhanced the STAT-6 mediated transcription activity. The interaction between STAT-6 and the p100 protein was mediated by the full-length of the SN-like domain, whereas individual fragments of SN-like domain showed no binding activity to STAT-6. In line with these results, the SN-like domain was directly engaged in the enhancement of STAT-6 mediated activation of gene transcription in vivo. Yet the TD domain had no ability to increase the transcription activation, but it was still required for the sufficient activation of transcription.展开更多
目的:探讨辛芷鼻敏胶囊对变应性鼻炎(Allergic rhinitis,AR)大鼠的血清中白介素13(Interleukin-13,IL-13)和鼻粘膜组织的信号转导和转录激活因子-6(Signaltransducers and activators of transcription-6,STAT6)mRNA及蛋白表达,明确药...目的:探讨辛芷鼻敏胶囊对变应性鼻炎(Allergic rhinitis,AR)大鼠的血清中白介素13(Interleukin-13,IL-13)和鼻粘膜组织的信号转导和转录激活因子-6(Signaltransducers and activators of transcription-6,STAT6)mRNA及蛋白表达,明确药效机制。方法:40只大鼠随机分为空白对照组、模型组、对照组、实验组,用卵清蛋白建立AR大鼠模型,通过对造模的大鼠灌服辛芷鼻敏胶囊及与同类药物对照,用酶联免疫吸附技术(Enzyme labeled immunosorbent assay,ELISA)定量测定各组大鼠血清IL-13水平,用原位杂交(ISH)、免疫组化方法半定量检测鼻粘膜中STAT6mRNA、STAT6蛋白含量的表达。结果:实验组大鼠血清的IL-13表达水平和鼻粘膜中STAT6mRNA、STAT6蛋白含量的表达比例明显低于模型组及对照组(P<0.05)。结论:辛芷鼻敏胶囊可能降低STAT6mRNA、STAT6蛋白含量表达,抑制大鼠AR发病过程中IL-13的表达水平,进而减少嗜酸性粒细胞浸润而控制症状。展开更多
文摘In the present study, the interaction of human p100 protein with signal transducer and activator of transcription-6 (STAT-6) was investigated. It was proved that the staphylococcal nuclease (SN)-like and tudor (TD) domains containing in p100 protein acting as a adaptor to recruit STAT-6 to the basal transcription machinery, enhanced the STAT-6 mediated transcription activity. The interaction between STAT-6 and the p100 protein was mediated by the full-length of the SN-like domain, whereas individual fragments of SN-like domain showed no binding activity to STAT-6. In line with these results, the SN-like domain was directly engaged in the enhancement of STAT-6 mediated activation of gene transcription in vivo. Yet the TD domain had no ability to increase the transcription activation, but it was still required for the sufficient activation of transcription.
文摘目的:探讨辛芷鼻敏胶囊对变应性鼻炎(Allergic rhinitis,AR)大鼠的血清中白介素13(Interleukin-13,IL-13)和鼻粘膜组织的信号转导和转录激活因子-6(Signaltransducers and activators of transcription-6,STAT6)mRNA及蛋白表达,明确药效机制。方法:40只大鼠随机分为空白对照组、模型组、对照组、实验组,用卵清蛋白建立AR大鼠模型,通过对造模的大鼠灌服辛芷鼻敏胶囊及与同类药物对照,用酶联免疫吸附技术(Enzyme labeled immunosorbent assay,ELISA)定量测定各组大鼠血清IL-13水平,用原位杂交(ISH)、免疫组化方法半定量检测鼻粘膜中STAT6mRNA、STAT6蛋白含量的表达。结果:实验组大鼠血清的IL-13表达水平和鼻粘膜中STAT6mRNA、STAT6蛋白含量的表达比例明显低于模型组及对照组(P<0.05)。结论:辛芷鼻敏胶囊可能降低STAT6mRNA、STAT6蛋白含量表达,抑制大鼠AR发病过程中IL-13的表达水平,进而减少嗜酸性粒细胞浸润而控制症状。
