To investigate the formation mechanism of stalk mechanical strength,clarify the mechanical mechanisms underlying stalk rot resistance,and establish core mechanical indicators for evaluating stalk strength,this study u...To investigate the formation mechanism of stalk mechanical strength,clarify the mechanical mechanisms underlying stalk rot resistance,and establish core mechanical indicators for evaluating stalk strength,this study utilized six maize varieties with different levels of stalk rot resistance including ZD619,HXD88,XZY801,XY696,LS1,and ZH431 as test materials.The density,moisture content,bending strength,rind puncture strength,and elastic modulus of maize stalks at different growth stages were determined,and their intrinsic relationships with stalk rot resistance were analyzed.Our results indicated that the stalk density,moisture content,bending strength,and rind puncture strength were responsive to stalk rot resistance.However,these indicators were in significant correlations with stalk rot resistance only after the physiological maturity of maize.In particular,stalk rind puncture strength exhibited the closest correlation with stalk rot resistance at the late stage of physiological maturity.Stalk density directly affected bending strength and rind puncture strength,and it indirectly affected the elastic modulus.The elastic modulus encompassing both load and displacement can improve the reliability of stalk strength evaluation,serving as the best indicator of stalk toughness.The attenuation amplitude of the elastic modulus was always the largest whether it was caused by the aggravation of the disease,the growth stage delay,or the internode position rise,which significantly improved the sensitivity of stalk strength evaluation.The elastic modulus of maize stalks was correlated with stalk rot resistance from the silking stage to late physiological maturity stage,which not only effectively improved the accuracy of stalk strength evaluation but also was beneficial to improving the efficiency of maize variety selection.In conclusion,the elastic modulus of maize stalks can be used to evaluate the maize stalk strength truly and accurately.Especially in the region with a high stalk rot incidence,it was beneficial to improving the breeding efficiency of the maize varieties suitable for mechanical harvesting.展开更多
Pythium stalk rot(PSR)is a destructive disease of maize,severely affecting yield and grain quality.The identification of quantitative trait loci(QTL)or genes for resistance to PSR forms the basis of diseaseresistant h...Pythium stalk rot(PSR)is a destructive disease of maize,severely affecting yield and grain quality.The identification of quantitative trait loci(QTL)or genes for resistance to PSR forms the basis of diseaseresistant hybrids breeding.In this study,a major QTL,Resistance to Pythium stalk rot 1(RPSR1),was identified from a set of recombinant inbred lines derived from MS71 and POP.Using a recombinant progeny testing strategy,RPSR1 was fine-mapped in a 472 kb interval.Through candidate gene expression,gene knock-down and knock-out studies,a leucine-rich repeat receptor-like kinase gene,PEP RECEPTOR 2(ZmPEPR2),was assigned as a PSR resistance gene.These results provide insights into the genetic architecture of resistance to PSR in maize,which should facilitate breeding maize for resistance to stalk rot.展开更多
Gibberella stalk rot(GSR)caused by Fusarium graminearum is one of the most devastating diseases of maize,seriously impacting maize yield and quality,as well as the ability to use technology of mechanical harvesting.En...Gibberella stalk rot(GSR)caused by Fusarium graminearum is one of the most devastating diseases of maize,seriously impacting maize yield and quality,as well as the ability to use technology of mechanical harvesting.Environmental conditions including photoperiod affect crop disease resistance.However,the mechanism underlying photoperiod-regulated maize GSR resistance remains unexplored.We found in this study that GSR resistance is regulated by the ZmPIF4.1(Phytochrome-Interacting Factor4)-ZmPTI1c(Pto-Interacting 1)-ZmMYB31 module coupled with photoperiod.The functional analysis of zmpti1c mutant indicated that ZmPTI1c negatively regulates maize GSR resistance.Short day promoted the disease progression in both zmpti1c and wild-type plants.ZmPTI1c promoter contains multiple predicted cis-acting elements for light responses.Yeast one-hybrid assay(Y1H),Electrophoretic mobility shift analysis(EMSA),and Dual-luciferase(LUC)reporter assays demonstrated that ZmPIF4.1 binds to the G-box in ZmPTI1c promoter and activates its expression.Moreover,expression levels of ZmPIF4 and ZmPTI1c were significantly higher under short day than under long day.ZmPTI1c interacted with and phosphorylated ZmMYB31.GSR resistance in zmmyb31 mutant was significantly increased than in wild type,indicating that ZmMYB31 also negatively regulated GSR resistance.Furthermore,ZmMYB31 suppressed the transcriptional activation of ZmPTI1c by ZmPIF4.1.Overall,ZmPIF4.1-ZmPTI1c-ZmMYB31negatively regulates maize immunity to GSR,which is likely modulated by photoperiod.展开更多
In view of the problems that the drilling depth can not be adjusted and the amount of liquid injection can not be accurately modulated in the local test device of maize variety breeding and disease resistance,combinin...In view of the problems that the drilling depth can not be adjusted and the amount of liquid injection can not be accurately modulated in the local test device of maize variety breeding and disease resistance,combining the test technical requirements of drilling,liquid injection and sealing of the penultimate radial pitch of maize straw from the ground,a quantitative adjustable liquid injection device for maize stalk center borehole was designed.Its structure,working principle,key technical parameters and practical application effect were elaborated in detail.The field experiment demonstrated that the quantitative adjustable liquid injection device for maize stalk center borehole could meet the requirements of the local test of maize stalk rot.展开更多
Carbonaceous cathode materials were prepared by a low-cost and facile molten salt carbonization of lotus stalks in molten carbonates at 850℃for aqueous zinc-ion hybrid supercapacitors(ZHSCs).The lotus stalk-derived c...Carbonaceous cathode materials were prepared by a low-cost and facile molten salt carbonization of lotus stalks in molten carbonates at 850℃for aqueous zinc-ion hybrid supercapacitors(ZHSCs).The lotus stalk-derived carbon by carbonization of one hour displayed excellent capacitive performance benefits from the comprehensive effect of hierarchically porous structure with large SSA,more mesopores,good electrical conductivity and high heteroatom doping.Coin-type ZHSCs deliver 164.4 F·g^(-1)at 0.2 A·g^(-1)and 70.0 F·g^(-1)at 20 A·g^(-1)with capacitance retention of 42.6%assembled with carbonic cathode and Zn@Zn_(3)(PO_(4))_(2)anode using 2 M ZnSO4 solution as electrolyte.Moreover,coin-type ZHSCs deliver the maximum energy density of 65.0 Wh·kg^(-1)at 168.7 W·kg^(-1)and the maximum power density of 11.4 kW·kg^(-1)at 12.7 Wh·kg^(-1).Thanks to the multifunctional Zn_(3)(PO_(4))_(2)interphase as Zn^(2+)-transfer ionic conductor and physical barrier.Moreover,coin-type ZHSCs exhibit outstanding recyclability with capacitance retention of 97.5%and coulombic efficiency of 100%after 10000 charge-discharge cycles at 1 A·g^(-1).展开更多
Anaerobic digestion has been defined as a competitive approach to facilitate the recycling of corn stalks.However,few studies have focused on the role of direct interspecies electron transfer(DIET)pathway in the acidi...Anaerobic digestion has been defined as a competitive approach to facilitate the recycling of corn stalks.However,few studies have focused on the role of direct interspecies electron transfer(DIET)pathway in the acidification stage under the addition of different particle sizes of zero-valent iron(ZVI).In this study,three types of ZVI,namely iron filings,iron powder and nanoscale iron,were investigated,respectively,to enhance its high-value conversion.Variations in volatile fatty acids(VFAs)and methane(CH4)production associated with the underlyingmechanisms were emphatically determined.Results indicated that the addition of ZVI could increase the concentration of VFAs,with the most outstanding performance observed with the use of nanoscale iron.Importantly,the conversion of propionic acid to acetic acid was driven by adding ZVI with no between-group differences in acidizing phase.Conversely,the substrate was more fully utilized when supplied with iron powder compared with other groups in methanogenic phase,thereby displaying the maximumCH4 yield with a value of 263.1 mL/(g total solids(TS)).However,adding nanoscale iron could signally shorten the digestion time(T80),saving 7 days in comparison to the group of iron powder.展开更多
Maize stalk lignin and cellulose contents are linked to lodging resistance,disease resistance,feed quality and ethanol conversion efficiency.After the six-leaf stage of maize(V6),these constituents are biosynthesized ...Maize stalk lignin and cellulose contents are linked to lodging resistance,disease resistance,feed quality and ethanol conversion efficiency.After the six-leaf stage of maize(V6),these constituents are biosynthesized and accumulated under the control of related enzymes and genes.However,the key enzymes,critical MYB transcription factors,and their dynamic alterations pattern under natural field circumstances are still unknown.Hence,we selected five cultivars with significant differences in lignocellulose content and lodging resistance as testing materials,performed field experiments for two years,and investigated the dynamics of lignin and cellulose content,related enzyme concentrations,and gene expression levels in the 3^(rd) and 5^(th) internodes above the ground after V6.The results showed that lignin and cellulose content increased after V6,stabilizing during the silking stage.This study identified COMT(caffeic acid 3-Omethyltransferase),TAL(tyrosine ammonia-lyase)and PAL(phenylalanine ammonia-lyase)as the key enzymes of lignin biosynthesis,while ZmCOMT,ZmCesA10 and ZmCesA8 were identified as essential genes.ZmMYB8,ZmMYB31 and ZmMYB39 were involved in regulating the expression of genes related to lignin synthesis,with ZmMYB31 potentially acting as a key negative regulator,while ZmMYB39 and ZmMYB8 acting as positive regulators.The study also found that around 14 d after V6 was a critical stage for regulating lignocellulose synthesis in the 3^(rd) to 5^(th) basal internode.This provides a theoretical foundation for developing regulatory techniques and breeding new cultivars to enhance lodging and disease resistance as well as the utility of maize stalks.展开更多
Maize stalk rot reduces grain yield and quality.Information about the genetics of resistance to maize stalk rot could help breeders design effective breeding strategies for the trait.Genomic prediction may be a more e...Maize stalk rot reduces grain yield and quality.Information about the genetics of resistance to maize stalk rot could help breeders design effective breeding strategies for the trait.Genomic prediction may be a more effective breeding strategy for stalk-rot resistance than marker-assisted selection.We performed a genome-wide association study(GWAS)and genomic prediction of resistance in testcross hybrids of 677 inbred lines from the Tuxpe?o and non-Tuxpe?o heterotic pools grown in three environments and genotyped with 200,681 single-nucleotide polymorphisms(SNPs).Eighteen SNPs associated with stalk rot shared genomic regions with gene families previously associated with plant biotic and abiotic responses.More favorable SNP haplotypes traced to tropical than to temperate progenitors of the inbred lines.Incorporating genotype-by-environment(G×E)interaction increased genomic prediction accuracy.展开更多
The demand for particleboard is increasing along with economic and population growth.However,two major barriers to the manufacture of particleboard are a shortage of raw materials(woodchips)and the emission of formald...The demand for particleboard is increasing along with economic and population growth.However,two major barriers to the manufacture of particleboard are a shortage of raw materials(woodchips)and the emission of formaldehyde from conventional adhesives.Agricultural by-products such as corn stalks contain an abundance of renewable lignocellulosic fiber.This study evaluates the effect of citric acid as a natural adhesive and fire retardant addition on the physical,mechanical,and fire retardancy properties of particleboards fabricated from corn stalks.A cost-effective and inorganic salt,calcium carbonate,was tested to enhance the fire retardancy.Ammonium dihydrogen phosphate was also considered as a comparative control.Particleboards with the addition of calcium carbonate was pretreated with sodium chloride.The particleboards were pressed for 10 min at 200℃.Japanese Industrial Standard JIS A 5908:2022 was used as the benchmark for the physical and mechanical tests.Fire retardancy was dynamically tested by simulating a Bushfire Attack Level of 19 kW/m^(2).The particleboard with 25 wt%citric acid had superior mechanical properties and complied with the JIS A 5908 standard for Type 13 base particleboard.Particleboard with the addition of calcium carbonate(5%and 10%)showed significantly delayed pyrolysis time.展开更多
BACKGROUND Pituitary stalk interruption syndrome(PSIS)is a rare anatomical defect of the pituitary gland falling under the spectrum of holoprosencephaly phenotypes.It is characterized by a deficiency in anterior pitui...BACKGROUND Pituitary stalk interruption syndrome(PSIS)is a rare anatomical defect of the pituitary gland falling under the spectrum of holoprosencephaly phenotypes.It is characterized by a deficiency in anterior pituitary hormones,such as growth hormone,gonadotropins,and thyroid hormones.Due to the syndrome's rarity and nonspecific manifestations,there is a lack of standardized treatment strategies.Consequently,early diagnosis through imaging and on-time intervention are crucial for improving patients’outcomes.CASE SUMMARY A 30-year-old man presented with absent secondary sexual characteristics and azoospermia.Laboratory evaluation revealed a deficiency in gonadotropins,while thyroid function was mostly within normal ranges.Magnetic resonance imaging of the pituitary gland showed pituitary stalk agenesis,hypoplasia of the anterior pituitary,and ectopic posterior pituitary,leading to the diagnosis of PSIS.Initially,the patient underwent 6 mo of gonadotropin therapy without significant changes in hormone levels and secondary sexual characteristics.Pulsatile gonadotropin-releasing hormone therapy was then administered,resulting in the detection of sperm in the semen analysis within 3 mo.After 6 mo,routine semen tests showed normal semen quality.The couple faced challenges in conceiving due to abstinence and underwent three cycles of artificial insemination,which was unsuccessful.They also attempted in vitro fertilization,but unfortunately,the woman experienced a miscarriage 10 wk after the embryo transfer.CONCLUSION Early detection,accurate diagnosis,and timely treatment are crucial in improving the quality of life and fertility of PSIS patients.展开更多
BACKGROUND Pituitary stalk interruption syndrome(PSIS)is a rare disorder,often characterized by delayed growth and development,short stature,and hypogonadism as the main clinical manifestations.It is not clear whether...BACKGROUND Pituitary stalk interruption syndrome(PSIS)is a rare disorder,often characterized by delayed growth and development,short stature,and hypogonadism as the main clinical manifestations.It is not clear whether PSIS can lead to liver cirrhosis.CASE SUMMARY This paper reported a case of liver cirrhosis of unknown origin.The patient was admitted to Beijing Ditan Hospital Affiliated to Capital Medical University in November 2023.The diagnosis of PSIS complicated with liver cirrhosis was established after a series of blood tests and pituitary magnetic resonance imaging examination.CONCLUSION We also reviewed the literature from both domestic and international sources to deepen the clinical understanding of PSIS in conjunction with liver cirrhosis among medical practitioners.展开更多
[Objective] To study the correlation between the biomechanical properties of rape stalks and rape stem lodging. [Method] Through axial compression tests to the stalks of 4 different rape varieties, the change rules of...[Objective] To study the correlation between the biomechanical properties of rape stalks and rape stem lodging. [Method] Through axial compression tests to the stalks of 4 different rape varieties, the change rules of maximum stem bearing ca- pacity, maximum compressive strength, elastic modulus and moment of inertia along plant height were analyzed, as well as the effect of different varieties and water contents on the biomechanical property indices of rape stalks. [Result] The maximum loads of rape stalks presented liner decrease trend along with the increase of stem height, and all reached the maximums below the height of 50 cm. The maximum stem compressive strength and elastic modulus of the 4 varieties were increased with ascending height, but in a slow rate with small change, thus the modulus of e- lasticity could be considered as unchanged. The maximum bearing capacity, maxi- mum compressive strength and elastic modulus of dry rape stalks were higher than wet stalks, indicating that the water contents of rape stalks had significant effect on their mechanical properties. According to the actual lodging situations in filed, stalks of variety No. 1 owned the worst biomechanical properties and lodging degree, while the biomechanical properties of No. 6 and F5 were better than No. 1 and No. 9, and they also had stronger lodging-resistance. [Conclusion] The study provides parameters and bases for the design of mechanized production and mechanical deep processing of crops, and can better reveal the physical natures of organisms. The methods used in this study can also be used to screen excellent crop stalks.展开更多
[Objective] The experiment was conducted to explore the suitable planting density and nitrogen amount for summer maize in Sichuan Basin with the objective to provide technical reservation and scientific basis for high...[Objective] The experiment was conducted to explore the suitable planting density and nitrogen amount for summer maize in Sichuan Basin with the objective to provide technical reservation and scientific basis for high-yielding cultivation technique.[Method] A widely planted maize cultivar ＇Chengdan 30＇ was used as experimental material to study the effects of planting density and nitrogen amount on the stalk agronomic traits,stalk lodging-resistance mechanical characters,stalk breaking percentage and yield of maize.Experiment was arranged in a two-factor split plot design with three replicates.The planting density was the main factor with three density gradients（4.5×10^4,6.0×10^4 and 7.5×10^4 plants/hm^2） and the nitrogen amount was the second factor with two different levels of nitrogen content（300 and 375 kg/hm^2）.[Result] The stalk lodging-resistance and yield were affected by planting density significantly.The increase of planting density would result in an increase of internode length and decrease of internode diameter,dry matter weight of per unit stalk length,rind penetration strength and breaking resistance of 3rd and 4th basal internodes.When planting density increased from 6.0×10^4 plants/hm2 to 7.5×10^4 plants/hm^2,the stalk breaking percentage in the whole growing season increased by 17.17%,and the yield reduced by 17.58%.The interaction between planting density and nitrogen amount affected the stalk breaking percentage in the whole growing season and yield significantly.The treatment with planting density of 6.0×104 plants/hm^2 and nitrogen amount of 375 kg/hm^2 of pure N was an optimal combination,which may not only control the stalk breaking percentage of whole growing stage effectively,but also could obtain an optimum grain yield.[Conclusion] In Sichuan Basin,the appropriate planting density and nitrogen amount for summer maize were 6.0×10^4 plants/hm^2 and 375 kg/hm^2.展开更多
[Objective] The study aimed at reducing the economic cost in feeding Tenebrio molitor and improving the utilization ratio of corn stalks.[Method] The effects of different proportions of corn stalks on the weigh of Ten...[Objective] The study aimed at reducing the economic cost in feeding Tenebrio molitor and improving the utilization ratio of corn stalks.[Method] The effects of different proportions of corn stalks on the weigh of Tenebrio molitor and the contents of crude protein,crude fat,Ca,P,dry matter and crude ash in the half-dry matter of Tenebrio molitor had been studied in the laboratory.[Result] Compared with the recipes of all wheat bran and pure fine fodder,feeding Tenebrio molitor by applying 30%-60% of corn stalks could significantly improve the content of crude protein in the half-dry materials of Tenebrio molitor,but it was detrimental to the improvement in weight of Tenebrio molitor and the accumulation of crude fat.To be specific,the recipe V（50% of corn stalks ＋ 50% of fine fodder） was beneficial to the accumulation of dry matter and crude ash.The recipe VII（70% of corn stalks ＋ 30% of fine fodder） and the recipe VIII（70% of corn stalks treated with Guangda feed leaven ＋ 30% of fine fodder） were beneficial to the accumulation of crude protein,Ca and P.There were differences among the influences of corn stalks in different treatments on the crude protein and other nutrition indicators of Tenebrio molitor.[Conclusion] Appropriately applying corn stalks can improve the accumulation of the crude protein,crude ash,dry matter,Ca and P of Tenebrio molitor but was detrimental to the growth and development of Tenebrio molitor and the accumulation of crude fat.展开更多
[Objective] This study was to explore the effects of dilute acid hydrolysis on fermentative biohydrogen production capacity of maize stalk. [Method] Using maize stalks subjected to mechanical disintegration,steam expl...[Objective] This study was to explore the effects of dilute acid hydrolysis on fermentative biohydrogen production capacity of maize stalk. [Method] Using maize stalks subjected to mechanical disintegration,steam explosion and dilute acid hydrolysis as experimental materials,we measured and analyzed the effects of different treatments and particle size of maize stalk were analyzed. [Result] The optimal fermentative biohydrogen production was found under following parameters：pretreatment of 0.8% dilute H2SO4 following steam explosion,particle size of maize stalk of 0.425-0.850 mm,liquid-solid ratio [0.8% H2SO4 （M）：stalk （W）] of 10：1. [Conclusion] Post steam explosion,dilute 0.8% dilute H2SO4 intensified hydrolysis on maize stalk could produce fermentative biohydrogen production capacity.展开更多
[Purpose]To research upon the structure and pyrolysis of cotton stalk.[Method] In this paper thermogravimetric analysis is adopted to study pyrolysis characteristics on the longitudinal direction of cotton stalk（the ...[Purpose]To research upon the structure and pyrolysis of cotton stalk.[Method] In this paper thermogravimetric analysis is adopted to study pyrolysis characteristics on the longitudinal direction of cotton stalk（the stick and the root）and on the traverse direction（the cuticle,cortex and medulla）.[Result] Cotton stalk is a material of uneven and irregular texture,and there are great differences in the structure of all these parts on the longitudinal and traverse directions,especially in the contents of different components in each layer.The reaction in the stick and root becomes violent along with the increase in the rising rate of temperature,and carbon yield rate decreases in turn.Besides with the same rising rate of temperature,carbon yield rate of cotton stick is higher than the one of cotton root.Carbon yield rates of cuticle,cortex and medulla decrease in turn while the violence of the reaction in cuticle is less severe than in cortex and medulla.[Conclusion] It has provided theoretical basis for obtaining activated carbon technology of cotton stick.展开更多
[Objective] This study aimed to study the adsorption performance of activated carbon prepared from corn stalks. [Methed] With granular activated carbon prepared from corn stalks as research object, adsorption performa...[Objective] This study aimed to study the adsorption performance of activated carbon prepared from corn stalks. [Methed] With granular activated carbon prepared from corn stalks as research object, adsorption performance simulation test equipment was set up to investigate the adsorption performance of the prepared activated carbon for methanol by static weight method. In addition, the effects of adsorption bed structure, activated carbon particle size in adsorption bed, addition amount of graphite powder in activated carbon and modified activated carbon on systematic adsorption performance were studied. [Result] Under conditions of same activated carbon and same adsorption temperature, the adsorption performance of new adsorption bed A （installed with finned diaphragm adsorbate tubes） was signifi- cantly better than that of unmodified adsorption bed B. Compared with adsorption bed B, adsorption bed A took 5 min shorter to reach the adsorption amount of 0.22 g/g. Under the same adsorption temperature, the adsorption performance of bed loaded with different-particle size activated carbon was significantly better than that loaded with same-particle size activated carbon. The bed loaded with different-particle size activated carbon took 16 min shorter to reach the adsorption capacity of 0.22 g/g compared with the bed loaded with same-particle size activated carbon. Adding proper amount of graphite powder in activated carbon could enhance the thermal conductivity and strengthen the adsorption properties. The optimum addition amount of graphite powder was 20% of the total amount of activated carbon. Com- pared with that of the control, the adsorption performance of activated carbon soaked by weak acidic solution was significantly improved. It took 3 min shorter to reach 87.1% of the equilibrium adsorption amount. [Conclusion] This study will provide reference for optimizing structural design of adsorption bed and adsorption refrigeration system.展开更多
In order to explore the method of returning corn stalk into field, the effects of returning corn stalk direcfly into soil and applying corn stalk-composted orgar^c fertilizer into soil on the physiochemical properties...In order to explore the method of returning corn stalk into field, the effects of returning corn stalk direcfly into soil and applying corn stalk-composted orgar^c fertilizer into soil on the physiochemical properties of tobacco-growing soil were studied. The results showed that returning corn stalk into soil could reduce soil bulk density and increase soil porosity, so as to improve the water and fertilizer retention capacity of tobacco-growing soil. At the same time, returning corn stalk into soil could also increase the organic matter, available phosphorus and available potassi- um contents in tobacco-growing soil. In the early field growth stage of tobacco, soil alkali-hydrolyzable nitrogen content decreased slightly; but in the middle and later field growth stage, the alkali-hydrolyzable nitrogen content in tobacco-growing soil increased. The treatment effect of corn stalk＋urea＋fermentation bacteria and corn stalk＋urea＋BM was better than that of the control （returning corn stalk directly into soil）, indicating that the corn stalk-composted organic fertilizer had certain popular- ization value.展开更多
文摘To investigate the formation mechanism of stalk mechanical strength,clarify the mechanical mechanisms underlying stalk rot resistance,and establish core mechanical indicators for evaluating stalk strength,this study utilized six maize varieties with different levels of stalk rot resistance including ZD619,HXD88,XZY801,XY696,LS1,and ZH431 as test materials.The density,moisture content,bending strength,rind puncture strength,and elastic modulus of maize stalks at different growth stages were determined,and their intrinsic relationships with stalk rot resistance were analyzed.Our results indicated that the stalk density,moisture content,bending strength,and rind puncture strength were responsive to stalk rot resistance.However,these indicators were in significant correlations with stalk rot resistance only after the physiological maturity of maize.In particular,stalk rind puncture strength exhibited the closest correlation with stalk rot resistance at the late stage of physiological maturity.Stalk density directly affected bending strength and rind puncture strength,and it indirectly affected the elastic modulus.The elastic modulus encompassing both load and displacement can improve the reliability of stalk strength evaluation,serving as the best indicator of stalk toughness.The attenuation amplitude of the elastic modulus was always the largest whether it was caused by the aggravation of the disease,the growth stage delay,or the internode position rise,which significantly improved the sensitivity of stalk strength evaluation.The elastic modulus of maize stalks was correlated with stalk rot resistance from the silking stage to late physiological maturity stage,which not only effectively improved the accuracy of stalk strength evaluation but also was beneficial to improving the efficiency of maize variety selection.In conclusion,the elastic modulus of maize stalks can be used to evaluate the maize stalk strength truly and accurately.Especially in the region with a high stalk rot incidence,it was beneficial to improving the breeding efficiency of the maize varieties suitable for mechanical harvesting.
基金supported by National Natural Science Foundation of China(32302371 to Junbin Chen)the National Key Research and Development Program,Ministry of Science and Technology of China(2022YFD1201802 to Wangsheng Zhu)Research Program from State Key Laboratory of Maize Biobreeding(SKLMB2424 to Wangsheng Zhu).
文摘Pythium stalk rot(PSR)is a destructive disease of maize,severely affecting yield and grain quality.The identification of quantitative trait loci(QTL)or genes for resistance to PSR forms the basis of diseaseresistant hybrids breeding.In this study,a major QTL,Resistance to Pythium stalk rot 1(RPSR1),was identified from a set of recombinant inbred lines derived from MS71 and POP.Using a recombinant progeny testing strategy,RPSR1 was fine-mapped in a 472 kb interval.Through candidate gene expression,gene knock-down and knock-out studies,a leucine-rich repeat receptor-like kinase gene,PEP RECEPTOR 2(ZmPEPR2),was assigned as a PSR resistance gene.These results provide insights into the genetic architecture of resistance to PSR in maize,which should facilitate breeding maize for resistance to stalk rot.
基金supported financially by the grants from the JBGS[2021]002 project from the Jiangsu Governmentthe National Nature Science Foundation of China(32472095)+2 种基金the National Key Research and Development Program of China(2020YFE02029002)Collaborative Innovation Center for Modern Crop Production(CIC-MCP)to Xiquan Gaosupported in part by the high-performance computing platform of Bioinformatics Center,Nanjing Agricultural University。
文摘Gibberella stalk rot(GSR)caused by Fusarium graminearum is one of the most devastating diseases of maize,seriously impacting maize yield and quality,as well as the ability to use technology of mechanical harvesting.Environmental conditions including photoperiod affect crop disease resistance.However,the mechanism underlying photoperiod-regulated maize GSR resistance remains unexplored.We found in this study that GSR resistance is regulated by the ZmPIF4.1(Phytochrome-Interacting Factor4)-ZmPTI1c(Pto-Interacting 1)-ZmMYB31 module coupled with photoperiod.The functional analysis of zmpti1c mutant indicated that ZmPTI1c negatively regulates maize GSR resistance.Short day promoted the disease progression in both zmpti1c and wild-type plants.ZmPTI1c promoter contains multiple predicted cis-acting elements for light responses.Yeast one-hybrid assay(Y1H),Electrophoretic mobility shift analysis(EMSA),and Dual-luciferase(LUC)reporter assays demonstrated that ZmPIF4.1 binds to the G-box in ZmPTI1c promoter and activates its expression.Moreover,expression levels of ZmPIF4 and ZmPTI1c were significantly higher under short day than under long day.ZmPTI1c interacted with and phosphorylated ZmMYB31.GSR resistance in zmmyb31 mutant was significantly increased than in wild type,indicating that ZmMYB31 also negatively regulated GSR resistance.Furthermore,ZmMYB31 suppressed the transcriptional activation of ZmPTI1c by ZmPIF4.1.Overall,ZmPIF4.1-ZmPTI1c-ZmMYB31negatively regulates maize immunity to GSR,which is likely modulated by photoperiod.
文摘In view of the problems that the drilling depth can not be adjusted and the amount of liquid injection can not be accurately modulated in the local test device of maize variety breeding and disease resistance,combining the test technical requirements of drilling,liquid injection and sealing of the penultimate radial pitch of maize straw from the ground,a quantitative adjustable liquid injection device for maize stalk center borehole was designed.Its structure,working principle,key technical parameters and practical application effect were elaborated in detail.The field experiment demonstrated that the quantitative adjustable liquid injection device for maize stalk center borehole could meet the requirements of the local test of maize stalk rot.
基金Funded by the Natural Science Foundation of Hubei Province(No.2024AFB930)the Scientific Research Project of Department of Education of Hubei Province(No.B2022096)+2 种基金the Scientific Research Project of Enshi Tujia&Miao Autonomous Prefecture(No.D20220073)the Open Fund of Hubei Key Laboratory of Biological Resources Protection and Utilization(Hubei Minzu University)(No.PT012210)the Hengsheng Industry Donation Fund(No.2025001)。
文摘Carbonaceous cathode materials were prepared by a low-cost and facile molten salt carbonization of lotus stalks in molten carbonates at 850℃for aqueous zinc-ion hybrid supercapacitors(ZHSCs).The lotus stalk-derived carbon by carbonization of one hour displayed excellent capacitive performance benefits from the comprehensive effect of hierarchically porous structure with large SSA,more mesopores,good electrical conductivity and high heteroatom doping.Coin-type ZHSCs deliver 164.4 F·g^(-1)at 0.2 A·g^(-1)and 70.0 F·g^(-1)at 20 A·g^(-1)with capacitance retention of 42.6%assembled with carbonic cathode and Zn@Zn_(3)(PO_(4))_(2)anode using 2 M ZnSO4 solution as electrolyte.Moreover,coin-type ZHSCs deliver the maximum energy density of 65.0 Wh·kg^(-1)at 168.7 W·kg^(-1)and the maximum power density of 11.4 kW·kg^(-1)at 12.7 Wh·kg^(-1).Thanks to the multifunctional Zn_(3)(PO_(4))_(2)interphase as Zn^(2+)-transfer ionic conductor and physical barrier.Moreover,coin-type ZHSCs exhibit outstanding recyclability with capacitance retention of 97.5%and coulombic efficiency of 100%after 10000 charge-discharge cycles at 1 A·g^(-1).
基金supported by the Fundamental Research Funds for the Central Universities of China(No.B220202066)the Natural Science Foundation of Jiangsu Province(No.BK20200527)the Anhui Provincial Key Laboratory of Environmental Pollution Control and Resource Reuse(No.2021EPC05).
文摘Anaerobic digestion has been defined as a competitive approach to facilitate the recycling of corn stalks.However,few studies have focused on the role of direct interspecies electron transfer(DIET)pathway in the acidification stage under the addition of different particle sizes of zero-valent iron(ZVI).In this study,three types of ZVI,namely iron filings,iron powder and nanoscale iron,were investigated,respectively,to enhance its high-value conversion.Variations in volatile fatty acids(VFAs)and methane(CH4)production associated with the underlyingmechanisms were emphatically determined.Results indicated that the addition of ZVI could increase the concentration of VFAs,with the most outstanding performance observed with the use of nanoscale iron.Importantly,the conversion of propionic acid to acetic acid was driven by adding ZVI with no between-group differences in acidizing phase.Conversely,the substrate was more fully utilized when supplied with iron powder compared with other groups in methanogenic phase,thereby displaying the maximumCH4 yield with a value of 263.1 mL/(g total solids(TS)).However,adding nanoscale iron could signally shorten the digestion time(T80),saving 7 days in comparison to the group of iron powder.
基金supported by the National Key Research and Development Program for Grain High yield Science and Technology Innovation Project of China (2016YFD0300307,2016YFD0300209,2017YFD0301704,2018YFD0301206).
文摘Maize stalk lignin and cellulose contents are linked to lodging resistance,disease resistance,feed quality and ethanol conversion efficiency.After the six-leaf stage of maize(V6),these constituents are biosynthesized and accumulated under the control of related enzymes and genes.However,the key enzymes,critical MYB transcription factors,and their dynamic alterations pattern under natural field circumstances are still unknown.Hence,we selected five cultivars with significant differences in lignocellulose content and lodging resistance as testing materials,performed field experiments for two years,and investigated the dynamics of lignin and cellulose content,related enzyme concentrations,and gene expression levels in the 3^(rd) and 5^(th) internodes above the ground after V6.The results showed that lignin and cellulose content increased after V6,stabilizing during the silking stage.This study identified COMT(caffeic acid 3-Omethyltransferase),TAL(tyrosine ammonia-lyase)and PAL(phenylalanine ammonia-lyase)as the key enzymes of lignin biosynthesis,while ZmCOMT,ZmCesA10 and ZmCesA8 were identified as essential genes.ZmMYB8,ZmMYB31 and ZmMYB39 were involved in regulating the expression of genes related to lignin synthesis,with ZmMYB31 potentially acting as a key negative regulator,while ZmMYB39 and ZmMYB8 acting as positive regulators.The study also found that around 14 d after V6 was a critical stage for regulating lignocellulose synthesis in the 3^(rd) to 5^(th) basal internode.This provides a theoretical foundation for developing regulatory techniques and breeding new cultivars to enhance lodging and disease resistance as well as the utility of maize stalks.
基金funded by the CGIAR Research Program(CRP)on MAIZEthe USAID through the Accelerating Genetic Gains Supplemental Project(Amend.No.9 MTO 069033),and the One CGIAR Initiative on Accelerated Breeding+1 种基金funding from the governments of Australia,Belgium,Canada,China,France,India,Japan,the Republic of Korea,Mexico,the Netherlands,New Zealand,Norway,Sweden,Switzerland,the United Kingdom,the United States,and the World Banksupported by the China Scholarship Council。
文摘Maize stalk rot reduces grain yield and quality.Information about the genetics of resistance to maize stalk rot could help breeders design effective breeding strategies for the trait.Genomic prediction may be a more effective breeding strategy for stalk-rot resistance than marker-assisted selection.We performed a genome-wide association study(GWAS)and genomic prediction of resistance in testcross hybrids of 677 inbred lines from the Tuxpe?o and non-Tuxpe?o heterotic pools grown in three environments and genotyped with 200,681 single-nucleotide polymorphisms(SNPs).Eighteen SNPs associated with stalk rot shared genomic regions with gene families previously associated with plant biotic and abiotic responses.More favorable SNP haplotypes traced to tropical than to temperate progenitors of the inbred lines.Incorporating genotype-by-environment(G×E)interaction increased genomic prediction accuracy.
文摘The demand for particleboard is increasing along with economic and population growth.However,two major barriers to the manufacture of particleboard are a shortage of raw materials(woodchips)and the emission of formaldehyde from conventional adhesives.Agricultural by-products such as corn stalks contain an abundance of renewable lignocellulosic fiber.This study evaluates the effect of citric acid as a natural adhesive and fire retardant addition on the physical,mechanical,and fire retardancy properties of particleboards fabricated from corn stalks.A cost-effective and inorganic salt,calcium carbonate,was tested to enhance the fire retardancy.Ammonium dihydrogen phosphate was also considered as a comparative control.Particleboards with the addition of calcium carbonate was pretreated with sodium chloride.The particleboards were pressed for 10 min at 200℃.Japanese Industrial Standard JIS A 5908:2022 was used as the benchmark for the physical and mechanical tests.Fire retardancy was dynamically tested by simulating a Bushfire Attack Level of 19 kW/m^(2).The particleboard with 25 wt%citric acid had superior mechanical properties and complied with the JIS A 5908 standard for Type 13 base particleboard.Particleboard with the addition of calcium carbonate(5%and 10%)showed significantly delayed pyrolysis time.
基金Weifang Fundamental Research Projects,No.WFWSJK-2023-052.
文摘BACKGROUND Pituitary stalk interruption syndrome(PSIS)is a rare anatomical defect of the pituitary gland falling under the spectrum of holoprosencephaly phenotypes.It is characterized by a deficiency in anterior pituitary hormones,such as growth hormone,gonadotropins,and thyroid hormones.Due to the syndrome's rarity and nonspecific manifestations,there is a lack of standardized treatment strategies.Consequently,early diagnosis through imaging and on-time intervention are crucial for improving patients’outcomes.CASE SUMMARY A 30-year-old man presented with absent secondary sexual characteristics and azoospermia.Laboratory evaluation revealed a deficiency in gonadotropins,while thyroid function was mostly within normal ranges.Magnetic resonance imaging of the pituitary gland showed pituitary stalk agenesis,hypoplasia of the anterior pituitary,and ectopic posterior pituitary,leading to the diagnosis of PSIS.Initially,the patient underwent 6 mo of gonadotropin therapy without significant changes in hormone levels and secondary sexual characteristics.Pulsatile gonadotropin-releasing hormone therapy was then administered,resulting in the detection of sperm in the semen analysis within 3 mo.After 6 mo,routine semen tests showed normal semen quality.The couple faced challenges in conceiving due to abstinence and underwent three cycles of artificial insemination,which was unsuccessful.They also attempted in vitro fertilization,but unfortunately,the woman experienced a miscarriage 10 wk after the embryo transfer.CONCLUSION Early detection,accurate diagnosis,and timely treatment are crucial in improving the quality of life and fertility of PSIS patients.
文摘BACKGROUND Pituitary stalk interruption syndrome(PSIS)is a rare disorder,often characterized by delayed growth and development,short stature,and hypogonadism as the main clinical manifestations.It is not clear whether PSIS can lead to liver cirrhosis.CASE SUMMARY This paper reported a case of liver cirrhosis of unknown origin.The patient was admitted to Beijing Ditan Hospital Affiliated to Capital Medical University in November 2023.The diagnosis of PSIS complicated with liver cirrhosis was established after a series of blood tests and pituitary magnetic resonance imaging examination.CONCLUSION We also reviewed the literature from both domestic and international sources to deepen the clinical understanding of PSIS in conjunction with liver cirrhosis among medical practitioners.
基金Supported by the Special Fund for Crop Breeding of Sichuan Provincial Department of Education,China (2006LD006)the Rapeseed Breeding Research Program of Science & Technology Department of Sichuan Province,China (2006YZGG-5-5)~~
文摘[Objective] To study the correlation between the biomechanical properties of rape stalks and rape stem lodging. [Method] Through axial compression tests to the stalks of 4 different rape varieties, the change rules of maximum stem bearing ca- pacity, maximum compressive strength, elastic modulus and moment of inertia along plant height were analyzed, as well as the effect of different varieties and water contents on the biomechanical property indices of rape stalks. [Result] The maximum loads of rape stalks presented liner decrease trend along with the increase of stem height, and all reached the maximums below the height of 50 cm. The maximum stem compressive strength and elastic modulus of the 4 varieties were increased with ascending height, but in a slow rate with small change, thus the modulus of e- lasticity could be considered as unchanged. The maximum bearing capacity, maxi- mum compressive strength and elastic modulus of dry rape stalks were higher than wet stalks, indicating that the water contents of rape stalks had significant effect on their mechanical properties. According to the actual lodging situations in filed, stalks of variety No. 1 owned the worst biomechanical properties and lodging degree, while the biomechanical properties of No. 6 and F5 were better than No. 1 and No. 9, and they also had stronger lodging-resistance. [Conclusion] The study provides parameters and bases for the design of mechanized production and mechanical deep processing of crops, and can better reveal the physical natures of organisms. The methods used in this study can also be used to screen excellent crop stalks.
基金Supported by the Special Fund for Agricultural and Rural Research in the Public Interest of Sichuan Province(12ZC1930)~~
文摘[Objective] The experiment was conducted to explore the suitable planting density and nitrogen amount for summer maize in Sichuan Basin with the objective to provide technical reservation and scientific basis for high-yielding cultivation technique.[Method] A widely planted maize cultivar ＇Chengdan 30＇ was used as experimental material to study the effects of planting density and nitrogen amount on the stalk agronomic traits,stalk lodging-resistance mechanical characters,stalk breaking percentage and yield of maize.Experiment was arranged in a two-factor split plot design with three replicates.The planting density was the main factor with three density gradients（4.5×10^4,6.0×10^4 and 7.5×10^4 plants/hm^2） and the nitrogen amount was the second factor with two different levels of nitrogen content（300 and 375 kg/hm^2）.[Result] The stalk lodging-resistance and yield were affected by planting density significantly.The increase of planting density would result in an increase of internode length and decrease of internode diameter,dry matter weight of per unit stalk length,rind penetration strength and breaking resistance of 3rd and 4th basal internodes.When planting density increased from 6.0×10^4 plants/hm2 to 7.5×10^4 plants/hm^2,the stalk breaking percentage in the whole growing season increased by 17.17%,and the yield reduced by 17.58%.The interaction between planting density and nitrogen amount affected the stalk breaking percentage in the whole growing season and yield significantly.The treatment with planting density of 6.0×104 plants/hm^2 and nitrogen amount of 375 kg/hm^2 of pure N was an optimal combination,which may not only control the stalk breaking percentage of whole growing stage effectively,but also could obtain an optimum grain yield.[Conclusion] In Sichuan Basin,the appropriate planting density and nitrogen amount for summer maize were 6.0×10^4 plants/hm^2 and 375 kg/hm^2.
基金Supported by National Special Fund for Scientific Research in Public Welfare Trade(Agriculture)(200803033-B0602-01)Project of Hebei Province Scientific and Technological Department(10221019)+1 种基金Project of Qinghuangdao City Science and Technology Burea of Hebei Province(201001A152)Foundation of Education Committee of Hebei Province(2003308)~~
文摘[Objective] The study aimed at reducing the economic cost in feeding Tenebrio molitor and improving the utilization ratio of corn stalks.[Method] The effects of different proportions of corn stalks on the weigh of Tenebrio molitor and the contents of crude protein,crude fat,Ca,P,dry matter and crude ash in the half-dry matter of Tenebrio molitor had been studied in the laboratory.[Result] Compared with the recipes of all wheat bran and pure fine fodder,feeding Tenebrio molitor by applying 30%-60% of corn stalks could significantly improve the content of crude protein in the half-dry materials of Tenebrio molitor,but it was detrimental to the improvement in weight of Tenebrio molitor and the accumulation of crude fat.To be specific,the recipe V（50% of corn stalks ＋ 50% of fine fodder） was beneficial to the accumulation of dry matter and crude ash.The recipe VII（70% of corn stalks ＋ 30% of fine fodder） and the recipe VIII（70% of corn stalks treated with Guangda feed leaven ＋ 30% of fine fodder） were beneficial to the accumulation of crude protein,Ca and P.There were differences among the influences of corn stalks in different treatments on the crude protein and other nutrition indicators of Tenebrio molitor.[Conclusion] Appropriately applying corn stalks can improve the accumulation of the crude protein,crude ash,dry matter,Ca and P of Tenebrio molitor but was detrimental to the growth and development of Tenebrio molitor and the accumulation of crude fat.
基金Supported by National Basic Research Program of China(2006CB708407 2009CB220005)+2 种基金National Natural Science Foun-dation of China (90610001 20871106)Program of 211 Projectfor Zhengzhou University from Ministry of Education~~
文摘[Objective] This study was to explore the effects of dilute acid hydrolysis on fermentative biohydrogen production capacity of maize stalk. [Method] Using maize stalks subjected to mechanical disintegration,steam explosion and dilute acid hydrolysis as experimental materials,we measured and analyzed the effects of different treatments and particle size of maize stalk were analyzed. [Result] The optimal fermentative biohydrogen production was found under following parameters：pretreatment of 0.8% dilute H2SO4 following steam explosion,particle size of maize stalk of 0.425-0.850 mm,liquid-solid ratio [0.8% H2SO4 （M）：stalk （W）] of 10：1. [Conclusion] Post steam explosion,dilute 0.8% dilute H2SO4 intensified hydrolysis on maize stalk could produce fermentative biohydrogen production capacity.
文摘[Purpose]To research upon the structure and pyrolysis of cotton stalk.[Method] In this paper thermogravimetric analysis is adopted to study pyrolysis characteristics on the longitudinal direction of cotton stalk（the stick and the root）and on the traverse direction（the cuticle,cortex and medulla）.[Result] Cotton stalk is a material of uneven and irregular texture,and there are great differences in the structure of all these parts on the longitudinal and traverse directions,especially in the contents of different components in each layer.The reaction in the stick and root becomes violent along with the increase in the rising rate of temperature,and carbon yield rate decreases in turn.Besides with the same rising rate of temperature,carbon yield rate of cotton stick is higher than the one of cotton root.Carbon yield rates of cuticle,cortex and medulla decrease in turn while the violence of the reaction in cuticle is less severe than in cortex and medulla.[Conclusion] It has provided theoretical basis for obtaining activated carbon technology of cotton stick.
基金Supported by Science and Technology Innovation Plan for Outstanding Scholars in Henan Province(2014KJCXJCRC015)~~
文摘[Objective] This study aimed to study the adsorption performance of activated carbon prepared from corn stalks. [Methed] With granular activated carbon prepared from corn stalks as research object, adsorption performance simulation test equipment was set up to investigate the adsorption performance of the prepared activated carbon for methanol by static weight method. In addition, the effects of adsorption bed structure, activated carbon particle size in adsorption bed, addition amount of graphite powder in activated carbon and modified activated carbon on systematic adsorption performance were studied. [Result] Under conditions of same activated carbon and same adsorption temperature, the adsorption performance of new adsorption bed A （installed with finned diaphragm adsorbate tubes） was signifi- cantly better than that of unmodified adsorption bed B. Compared with adsorption bed B, adsorption bed A took 5 min shorter to reach the adsorption amount of 0.22 g/g. Under the same adsorption temperature, the adsorption performance of bed loaded with different-particle size activated carbon was significantly better than that loaded with same-particle size activated carbon. The bed loaded with different-particle size activated carbon took 16 min shorter to reach the adsorption capacity of 0.22 g/g compared with the bed loaded with same-particle size activated carbon. Adding proper amount of graphite powder in activated carbon could enhance the thermal conductivity and strengthen the adsorption properties. The optimum addition amount of graphite powder was 20% of the total amount of activated carbon. Com- pared with that of the control, the adsorption performance of activated carbon soaked by weak acidic solution was significantly improved. It took 3 min shorter to reach 87.1% of the equilibrium adsorption amount. [Conclusion] This study will provide reference for optimizing structural design of adsorption bed and adsorption refrigeration system.
文摘In order to explore the method of returning corn stalk into field, the effects of returning corn stalk direcfly into soil and applying corn stalk-composted orgar^c fertilizer into soil on the physiochemical properties of tobacco-growing soil were studied. The results showed that returning corn stalk into soil could reduce soil bulk density and increase soil porosity, so as to improve the water and fertilizer retention capacity of tobacco-growing soil. At the same time, returning corn stalk into soil could also increase the organic matter, available phosphorus and available potassi- um contents in tobacco-growing soil. In the early field growth stage of tobacco, soil alkali-hydrolyzable nitrogen content decreased slightly; but in the middle and later field growth stage, the alkali-hydrolyzable nitrogen content in tobacco-growing soil increased. The treatment effect of corn stalk＋urea＋fermentation bacteria and corn stalk＋urea＋BM was better than that of the control （returning corn stalk directly into soil）, indicating that the corn stalk-composted organic fertilizer had certain popular- ization value.
