Objective:To examine the chemical variability in inflorescences of wild populations of Stachys lavandulifolia Vahl(S.lavandulifolia)collected throughout two provinces(lsfahan and Chaharmahal va Bakhtiary),Southwest Ir...Objective:To examine the chemical variability in inflorescences of wild populations of Stachys lavandulifolia Vahl(S.lavandulifolia)collected throughout two provinces(lsfahan and Chaharmahal va Bakhtiary),Southwest Iran.Methods:The essential oils of S.lavandulifolia Vahl from seven locations were obtained by hydro-distillation and analysed by gas chromatography and gas chromatography-mass spectrometry.Results:The results revealed that distinct differences in the content of compounds depending on region of sample collection.The main constituents of the essential oils wereα-thujone(0.3%-32.3%),α-pinene(trace to 37.3%),myrcene(0.5%-15.9%),β-phellandrene(1.1%-37.9%),germacrene D(0.4%-11.3%),△-cadinene(trace to 11.6%)and 1,4-methano-1 H-indene(trace to 10.1%).Conclusions:The results of the present study indicated that essential oil components of S.lavandulifolia Vahl can be varied with genetic(ecotype),environmental conditions and geographic origin,In general,the essential oils of various populations of S.lavandulifolia Vahl were rich in monoterpenoids and sesquiterpenoids.展开更多
Objective: To evaluate the antioxidant activity of extracts and fractions from Stachys sieboldii Miq., and to examine its effect on the cellular reactive oxygen species(ROS) and glutathione(GSH) production and genomic...Objective: To evaluate the antioxidant activity of extracts and fractions from Stachys sieboldii Miq., and to examine its effect on the cellular reactive oxygen species(ROS) and glutathione(GSH) production and genomic DNA oxidation in HT-1080 cells. Methods: The ROS generation induced by H2 O2 was measured by the dichlorofluorescein-diacetate assay. GSH levels were measured using a fluorescent method with mBBr. Genomic DNA oxidative damage was measured with levels of oxidative DNA induced by the reaction of ferritin with H2 O2. Results: The n-hexane, 85% aqueous methanol and n-butanol fractions(0.05 mg/mL concentrations) inhibited H2 O2-induced ROS generation by 63%, 35% and 45%, respectively. GSH levels were significantly increased in both acetone+methylene chloride and methanol extracts(P<0.05). Supplementation of cells with n-hexane significantly increased GSH levels at concentrations of 0.05 mg/mL(P<0.05). Both the acetone+methylene chloride and methanol extracts, as well as all fractions significantly inhibited oxidative DNA damage(P<0.05). Conclusions: These results indicate that cellular oxidation was inhibited by the n-hexane fraction and this fraction may contain valuable active compounds.展开更多
Graphene oxide (GO) reduced by Stachys lavandulifolia extract (SLE) was produced and characterised. The anti-corrosion behaviour of epoxy coatings containing GO and rGO nanosheets was investigated. FESEM-EDS, FT-IR, a...Graphene oxide (GO) reduced by Stachys lavandulifolia extract (SLE) was produced and characterised. The anti-corrosion behaviour of epoxy coatings containing GO and rGO nanosheets was investigated. FESEM-EDS, FT-IR, and Raman spectroscopy were used to examine the microstructure and chemical composition of the nanosheets and epoxy coatings. EIS experiment was used to explore the corrosion behaviour of the coatings. The O/C ratio for GO and rGO-SLE was found to be 2.5 and 4.5, indicating a decrease in the carbon content after the reduction of GO, confirming the adsorption of SLE onto the GO nanosheets. The successful reduction of GO in the presence of SLE particles was confirmed by disappearing the C=O peak and a significant decrease in the C-O-C bond intensity. The epoxy/rGO- SLE coatings exhibited the highest double-layer thickness and excellent corrosion resistance compared to neat epoxy and epoxy/GO coatings, emphasizing the significant role of rGO in enhancing the protective performance of epoxy coatings. The highest values for total charge transfer and film resistances and the inhibition efficiency were observed to be 6529 Ω·cm^(2) and 90%, respectively, for the epoxy/rGO-SLE coated steel plate. It was also found that the epoxy/0.15 wt.% rGO-SLE coating demonstrates the best corrosion resistance performance.展开更多
The synthesis of nontoxic stable gold nanoparticles is important for medical applications. An aqueous extract of the plant Stachys lavandulifolia Vahl was used to synthesize gold nanoparticles. This green method invol...The synthesis of nontoxic stable gold nanoparticles is important for medical applications. An aqueous extract of the plant Stachys lavandulifolia Vahl was used to synthesize gold nanoparticles. This green method involved the S. lavandulifolia Vahl extract acting as a reducing and stabilizing agent. The nanoparticles were characterized by transmission electron microscopy, dynamic light scattering analysis and UV-vis absorption and Fourier transform-infrared spectroscopies. Stability under physiological conditions is important for medical applications. The stability of the nanoparticles was compared with that of conventional citrate-capped nanoparticles, under both synthetic and physiological conditions. The nanoparticles synthesized from the S. lavandulifolia Vahl extract were stable under physiological conditions, in contrast with conventional citrate-capped nanoparticles.展开更多
Unconventional vegetables, in general, are plants that have been largely consumed by the population at some point and, because of changes in eating behavior, now present reduced economic and social expression and have...Unconventional vegetables, in general, are plants that have been largely consumed by the population at some point and, because of changes in eating behavior, now present reduced economic and social expression and have lost ground to other vegetables. The objectives of this study were to perform phytochemical screening of the ethanol extracts of Rumex acetosa L., Tropaeolum majus L., Solanum muricatum, Stachys byzantina K. Koch and Solanum betaceum Cav. and evaluate their antioxidant potentials via the methods involving scavaging of the DPPH free radical and the ABTS radical, phosphomolybdenum and reducing power. In phytochemical screening, five species of unconventional vegetables tested positive for tannins;for sesquiterpene, lactones and other lactones. These tests were positive for Tropaeolum majus L. and Rumex acetosa L., Solanum betaceum Cav. and Solanum muricatum tested negative for steroids. Only Solanum betaceum Cav. gave positive tests for flavonoids. Among the five plant species studied, Stachys byzantina K. Koch presented the greatest antioxidant potential in all the methods evaluated.展开更多
基金Supported by deputy of Researches and Technology.Islamic Azad University of Shahrckord Branch.Iran(Grant no.IAUSHK:6121)
文摘Objective:To examine the chemical variability in inflorescences of wild populations of Stachys lavandulifolia Vahl(S.lavandulifolia)collected throughout two provinces(lsfahan and Chaharmahal va Bakhtiary),Southwest Iran.Methods:The essential oils of S.lavandulifolia Vahl from seven locations were obtained by hydro-distillation and analysed by gas chromatography and gas chromatography-mass spectrometry.Results:The results revealed that distinct differences in the content of compounds depending on region of sample collection.The main constituents of the essential oils wereα-thujone(0.3%-32.3%),α-pinene(trace to 37.3%),myrcene(0.5%-15.9%),β-phellandrene(1.1%-37.9%),germacrene D(0.4%-11.3%),△-cadinene(trace to 11.6%)and 1,4-methano-1 H-indene(trace to 10.1%).Conclusions:The results of the present study indicated that essential oil components of S.lavandulifolia Vahl can be varied with genetic(ecotype),environmental conditions and geographic origin,In general,the essential oils of various populations of S.lavandulifolia Vahl were rich in monoterpenoids and sesquiterpenoids.
基金supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2017R1A2B4005915)
文摘Objective: To evaluate the antioxidant activity of extracts and fractions from Stachys sieboldii Miq., and to examine its effect on the cellular reactive oxygen species(ROS) and glutathione(GSH) production and genomic DNA oxidation in HT-1080 cells. Methods: The ROS generation induced by H2 O2 was measured by the dichlorofluorescein-diacetate assay. GSH levels were measured using a fluorescent method with mBBr. Genomic DNA oxidative damage was measured with levels of oxidative DNA induced by the reaction of ferritin with H2 O2. Results: The n-hexane, 85% aqueous methanol and n-butanol fractions(0.05 mg/mL concentrations) inhibited H2 O2-induced ROS generation by 63%, 35% and 45%, respectively. GSH levels were significantly increased in both acetone+methylene chloride and methanol extracts(P<0.05). Supplementation of cells with n-hexane significantly increased GSH levels at concentrations of 0.05 mg/mL(P<0.05). Both the acetone+methylene chloride and methanol extracts, as well as all fractions significantly inhibited oxidative DNA damage(P<0.05). Conclusions: These results indicate that cellular oxidation was inhibited by the n-hexane fraction and this fraction may contain valuable active compounds.
文摘Graphene oxide (GO) reduced by Stachys lavandulifolia extract (SLE) was produced and characterised. The anti-corrosion behaviour of epoxy coatings containing GO and rGO nanosheets was investigated. FESEM-EDS, FT-IR, and Raman spectroscopy were used to examine the microstructure and chemical composition of the nanosheets and epoxy coatings. EIS experiment was used to explore the corrosion behaviour of the coatings. The O/C ratio for GO and rGO-SLE was found to be 2.5 and 4.5, indicating a decrease in the carbon content after the reduction of GO, confirming the adsorption of SLE onto the GO nanosheets. The successful reduction of GO in the presence of SLE particles was confirmed by disappearing the C=O peak and a significant decrease in the C-O-C bond intensity. The epoxy/rGO- SLE coatings exhibited the highest double-layer thickness and excellent corrosion resistance compared to neat epoxy and epoxy/GO coatings, emphasizing the significant role of rGO in enhancing the protective performance of epoxy coatings. The highest values for total charge transfer and film resistances and the inhibition efficiency were observed to be 6529 Ω·cm^(2) and 90%, respectively, for the epoxy/rGO-SLE coated steel plate. It was also found that the epoxy/0.15 wt.% rGO-SLE coating demonstrates the best corrosion resistance performance.
文摘The synthesis of nontoxic stable gold nanoparticles is important for medical applications. An aqueous extract of the plant Stachys lavandulifolia Vahl was used to synthesize gold nanoparticles. This green method involved the S. lavandulifolia Vahl extract acting as a reducing and stabilizing agent. The nanoparticles were characterized by transmission electron microscopy, dynamic light scattering analysis and UV-vis absorption and Fourier transform-infrared spectroscopies. Stability under physiological conditions is important for medical applications. The stability of the nanoparticles was compared with that of conventional citrate-capped nanoparticles, under both synthetic and physiological conditions. The nanoparticles synthesized from the S. lavandulifolia Vahl extract were stable under physiological conditions, in contrast with conventional citrate-capped nanoparticles.
基金The authors thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)the Coordenacao de Aperfeiçoamento de Pessoal de Nível Superior(CAPES) the Fundacao de AmparoàPesquisa do Estado de Minas Gerais(FAPEMIG)for financial support,a scholarship and a PVNS fellowship(D.L.Nelson).
文摘Unconventional vegetables, in general, are plants that have been largely consumed by the population at some point and, because of changes in eating behavior, now present reduced economic and social expression and have lost ground to other vegetables. The objectives of this study were to perform phytochemical screening of the ethanol extracts of Rumex acetosa L., Tropaeolum majus L., Solanum muricatum, Stachys byzantina K. Koch and Solanum betaceum Cav. and evaluate their antioxidant potentials via the methods involving scavaging of the DPPH free radical and the ABTS radical, phosphomolybdenum and reducing power. In phytochemical screening, five species of unconventional vegetables tested positive for tannins;for sesquiterpene, lactones and other lactones. These tests were positive for Tropaeolum majus L. and Rumex acetosa L., Solanum betaceum Cav. and Solanum muricatum tested negative for steroids. Only Solanum betaceum Cav. gave positive tests for flavonoids. Among the five plant species studied, Stachys byzantina K. Koch presented the greatest antioxidant potential in all the methods evaluated.
