Methicillin-resistant Staphylococcus aureus(MRSA)ST398 is a well-known livestock MRSA(LA-MRSA)in Europe.Notably,it has been increasingly detected in China recently.Food serves as a significant reservoir for S.aureus,a...Methicillin-resistant Staphylococcus aureus(MRSA)ST398 is a well-known livestock MRSA(LA-MRSA)in Europe.Notably,it has been increasingly detected in China recently.Food serves as a significant reservoir for S.aureus,and its transmission ability of MRSA ST398 remains to be explored in China.Recently,we screened four MRSA ST398 isolates from food sources in Shanghai,China,and subsequently performed whole-genome sequencing to identify their resistome and transmission potential.Phylogenetic analysis of these four MRSA ST398 isolates and other ST398 strains from China revealed that food serves as a significant vector for MRSA transmission among humans.Moreover,the four isolates in our study and infection-borne strains were classified within Clade 4.1,indicating their close genetic relationship.The most common antimicrobial resistance genes in Clade 4.1 isolates were mecA,blaZ,and ermC.Specifically,all mecA genes were located on SCCmec elements,which were excised from the genomes at a frequency of approximately 1×10^(-5).By prediction in silico,88.5%of the blaZ and 74.4% of the ermC genes were located on mobilizable plasmids.Overall,these findings indicated that SCCmec and plasmids are critical drivers for resistome transmission of MRSA ST398.Consequently,targeting mobile genetic elements could serve as an effective strategy for preventing the spread of antimicrobial resistance in S.aureus.展开更多
Background：Staphylococcus aureus is one of the predominant causes of skin and soft tissue infections （SSTIs）,but limited data were available regarding the characterization of S.aureus from SSTIs patients in Jiangsu...Background：Staphylococcus aureus is one of the predominant causes of skin and soft tissue infections （SSTIs）,but limited data were available regarding the characterization of S.aureus from SSTIs patients in Jiangsu Province in China.We aimed to investigate the molecular epidemiology ofS.aureus among SSTIs patients in two hospitals of Jiangsu Province.Methods：Sixty-two patients with SSTIs from two Chinese hospitals in Jiangsu Province were enrolled in this study,and 62 S.aureus isolates were collected from February 2014 to January 2015.S.aureus isolates were characterized by antimicrobial susceptibility testing,toxin gene detection,and molecular typing with sequence type,Staphylococcus protein A gene type,accessorygeneregulator（agr）group,and Staphylococcal cassette chromosome mec type.Results：Sixteen （25.8%） methicillin-resistant S.aureus （MRSA） isolates were detected,and there was no isolate found resistant to vancomycin,teicoplanin,sulfamethoxazole-trimethoprim,and linezolid.The sei was the toxin gene most frequently found,and no lukS/F-PV-positive isolates were detected among the SSTIs＆#39; patients.Molecular analysis revealed that ST398 （10/62,16.1%;2 MRSA and 8 methicillin-susceptible S.aureus） to be the dominant clone,followed by ST5 （8/62,12.9%） and ST7 （8/62,12.9%）.Conclusions：The livestock ST398 was the most common clone among patients with S.aureus SSTIs in Jiangsu Province,China.Surveillance and further studies on the important livestock ST398 clone in human infections are necessarily requested.展开更多
基金supported by the National Key R&D program of China(No.2024YFE0199000)the National Natural Science Foundation of China(No.32472458).
文摘Methicillin-resistant Staphylococcus aureus(MRSA)ST398 is a well-known livestock MRSA(LA-MRSA)in Europe.Notably,it has been increasingly detected in China recently.Food serves as a significant reservoir for S.aureus,and its transmission ability of MRSA ST398 remains to be explored in China.Recently,we screened four MRSA ST398 isolates from food sources in Shanghai,China,and subsequently performed whole-genome sequencing to identify their resistome and transmission potential.Phylogenetic analysis of these four MRSA ST398 isolates and other ST398 strains from China revealed that food serves as a significant vector for MRSA transmission among humans.Moreover,the four isolates in our study and infection-borne strains were classified within Clade 4.1,indicating their close genetic relationship.The most common antimicrobial resistance genes in Clade 4.1 isolates were mecA,blaZ,and ermC.Specifically,all mecA genes were located on SCCmec elements,which were excised from the genomes at a frequency of approximately 1×10^(-5).By prediction in silico,88.5%of the blaZ and 74.4% of the ermC genes were located on mobilizable plasmids.Overall,these findings indicated that SCCmec and plasmids are critical drivers for resistome transmission of MRSA ST398.Consequently,targeting mobile genetic elements could serve as an effective strategy for preventing the spread of antimicrobial resistance in S.aureus.
基金This work was supported by a grant from the National Natural Science Foundation of China （No. 81472010）.
文摘Background：Staphylococcus aureus is one of the predominant causes of skin and soft tissue infections （SSTIs）,but limited data were available regarding the characterization of S.aureus from SSTIs patients in Jiangsu Province in China.We aimed to investigate the molecular epidemiology ofS.aureus among SSTIs patients in two hospitals of Jiangsu Province.Methods：Sixty-two patients with SSTIs from two Chinese hospitals in Jiangsu Province were enrolled in this study,and 62 S.aureus isolates were collected from February 2014 to January 2015.S.aureus isolates were characterized by antimicrobial susceptibility testing,toxin gene detection,and molecular typing with sequence type,Staphylococcus protein A gene type,accessorygeneregulator（agr）group,and Staphylococcal cassette chromosome mec type.Results：Sixteen （25.8%） methicillin-resistant S.aureus （MRSA） isolates were detected,and there was no isolate found resistant to vancomycin,teicoplanin,sulfamethoxazole-trimethoprim,and linezolid.The sei was the toxin gene most frequently found,and no lukS/F-PV-positive isolates were detected among the SSTIs＆#39; patients.Molecular analysis revealed that ST398 （10/62,16.1%;2 MRSA and 8 methicillin-susceptible S.aureus） to be the dominant clone,followed by ST5 （8/62,12.9%） and ST7 （8/62,12.9%）.Conclusions：The livestock ST398 was the most common clone among patients with S.aureus SSTIs in Jiangsu Province,China.Surveillance and further studies on the important livestock ST398 clone in human infections are necessarily requested.
