背景:国际高危克隆大肠埃希菌(Escherichia coli) ST131是重要的医院获得性病原体,会导致尿路感染,血流感染以及呼吸道感染等,甚至死亡,给全球健康带来重大威胁。目的:探究ST131菌株的群体遗传特征,为控制大肠埃希菌抗生素耐药问题提供...背景:国际高危克隆大肠埃希菌(Escherichia coli) ST131是重要的医院获得性病原体,会导致尿路感染,血流感染以及呼吸道感染等,甚至死亡,给全球健康带来重大威胁。目的:探究ST131菌株的群体遗传特征,为控制大肠埃希菌抗生素耐药问题提供更多见解。结果:本研究收集2014~2023年来自全球的7735株ST131菌株全基因组测序数据,对其基因组特征进行了分析发现sul1 (54.6%)、mph(A) (53.3%)、tet(A) (52.5%)、dfrA17 (49.4%)、sul2 (38.4%)、aph(3'')-Ib (38.0%),aph(6)-Id (37.6%),blaTEM-1B (37.2%),blaCTX-M-15 (37.2%)是全球ST131菌株的优势磺胺类、大环内酯类、四环素类、甲氧苄啶类、氨基糖苷类、β-内酰胺类抗生素耐药基因(ARGs)变体。blaCTX-M-27、blaNDM-5、blaKPC-2、blaKPC-3的检出率随时间呈升高趋势;而aac(3)-IId、aac(6')-Ib-cr、blaOXA-1、catB3的检出呈下降趋势;同时aadA5、aph(3'')-Ib、aph(6)-Id、blaTEM-1B、dfrA17、mph(A)、tet(A)的检出呈波动变化(先升高再降低)。O25:H4是ST131的优势血清型。共检出543种毒力基因(VGs),与菌株粘附、定植等功能有关。抗生素耐药基因(ARGs)和毒力基因(VGs)的数量随时间增加而增多(P Background: The international high-risk clone Escherichia coli (ST131) is an important hospital-acquired pathogen that causes urinary tract infections, bloodstream infections, and respiratory infections, among other things, and even death, posing a major threat to global health. Objective: To explore the population genetic characteristics of ST131 strains and provide more insights into the problem of controlling antibiotic resistance in Escherichia coli. Results: In this study, we collected whole genome sequencing data of 7735 ST131 strains from around the world from 2014 to 2023 and analysed their genomic characteristics, found that sul1 (54.6%), mph(A) (53.3%), tet(A) (52.5%), dfrA17 (49.4%), sul2 (38.4%), aph(3'')-Ib (38.0%), aph(6)-Id (37.6%), blaTEM-1B (37.2%), blaCTX-M-15 (37.2%) are the dominant antibiotic-resistant gene(ARGs) variants of sulfonamides, macrolides, tetracyclines, mephedrone, aminoglycosides, and β-lactams for the global ST131 strain. blaCTX-M-27, blaNDM-5, blaKPC-2, blaKPC-3 showed an increasing trend in detection over time;while aac(3)-IId, aac(6')-Ib-cr, blaOXA-1, catB3 showed a decreasing trend in detection, while aadA5, aph(3'')-Ib, aph(6)-Id, blaTEM-1B, dfrA17, mph(A), tet(A) were detected with a fluctuating change (increasing and then decreasing). O25:H4 was the dominant serotype of ST131. A total of 543 virulence genes (VGs) were detected, which were related to the functions of strain adhesion and colonisation. The number of ARGs and VGs increased over time (P Escherichia coli ST131 continues to spread globally, with strains evolving towards high drug resistance and virulence and is posing a significant threat to clinical care.展开更多
On July 2,2002, Bureau of International Exhibition’s (BIE) 131st General Assemblywas convened in Paris. The assembly examined the investigation report by BIE InvestigationDelegation for those application countries. V...On July 2,2002, Bureau of International Exhibition’s (BIE) 131st General Assemblywas convened in Paris. The assembly examined the investigation report by BIE InvestigationDelegation for those application countries. Various application countries stated reportsrespectively.The Chinese Government Delegation headed by State Councilor Wu Yi attended the assembly, and stated report on China’s Shanghai making application to hold 2010 World Fair.展开更多
文摘背景:国际高危克隆大肠埃希菌(Escherichia coli) ST131是重要的医院获得性病原体,会导致尿路感染,血流感染以及呼吸道感染等,甚至死亡,给全球健康带来重大威胁。目的:探究ST131菌株的群体遗传特征,为控制大肠埃希菌抗生素耐药问题提供更多见解。结果:本研究收集2014~2023年来自全球的7735株ST131菌株全基因组测序数据,对其基因组特征进行了分析发现sul1 (54.6%)、mph(A) (53.3%)、tet(A) (52.5%)、dfrA17 (49.4%)、sul2 (38.4%)、aph(3'')-Ib (38.0%),aph(6)-Id (37.6%),blaTEM-1B (37.2%),blaCTX-M-15 (37.2%)是全球ST131菌株的优势磺胺类、大环内酯类、四环素类、甲氧苄啶类、氨基糖苷类、β-内酰胺类抗生素耐药基因(ARGs)变体。blaCTX-M-27、blaNDM-5、blaKPC-2、blaKPC-3的检出率随时间呈升高趋势;而aac(3)-IId、aac(6')-Ib-cr、blaOXA-1、catB3的检出呈下降趋势;同时aadA5、aph(3'')-Ib、aph(6)-Id、blaTEM-1B、dfrA17、mph(A)、tet(A)的检出呈波动变化(先升高再降低)。O25:H4是ST131的优势血清型。共检出543种毒力基因(VGs),与菌株粘附、定植等功能有关。抗生素耐药基因(ARGs)和毒力基因(VGs)的数量随时间增加而增多(P Background: The international high-risk clone Escherichia coli (ST131) is an important hospital-acquired pathogen that causes urinary tract infections, bloodstream infections, and respiratory infections, among other things, and even death, posing a major threat to global health. Objective: To explore the population genetic characteristics of ST131 strains and provide more insights into the problem of controlling antibiotic resistance in Escherichia coli. Results: In this study, we collected whole genome sequencing data of 7735 ST131 strains from around the world from 2014 to 2023 and analysed their genomic characteristics, found that sul1 (54.6%), mph(A) (53.3%), tet(A) (52.5%), dfrA17 (49.4%), sul2 (38.4%), aph(3'')-Ib (38.0%), aph(6)-Id (37.6%), blaTEM-1B (37.2%), blaCTX-M-15 (37.2%) are the dominant antibiotic-resistant gene(ARGs) variants of sulfonamides, macrolides, tetracyclines, mephedrone, aminoglycosides, and β-lactams for the global ST131 strain. blaCTX-M-27, blaNDM-5, blaKPC-2, blaKPC-3 showed an increasing trend in detection over time;while aac(3)-IId, aac(6')-Ib-cr, blaOXA-1, catB3 showed a decreasing trend in detection, while aadA5, aph(3'')-Ib, aph(6)-Id, blaTEM-1B, dfrA17, mph(A), tet(A) were detected with a fluctuating change (increasing and then decreasing). O25:H4 was the dominant serotype of ST131. A total of 543 virulence genes (VGs) were detected, which were related to the functions of strain adhesion and colonisation. The number of ARGs and VGs increased over time (P Escherichia coli ST131 continues to spread globally, with strains evolving towards high drug resistance and virulence and is posing a significant threat to clinical care.
文摘On July 2,2002, Bureau of International Exhibition’s (BIE) 131st General Assemblywas convened in Paris. The assembly examined the investigation report by BIE InvestigationDelegation for those application countries. Various application countries stated reportsrespectively.The Chinese Government Delegation headed by State Councilor Wu Yi attended the assembly, and stated report on China’s Shanghai making application to hold 2010 World Fair.
