"SSTL"(student-subject and teacher-leader),即"学生为主体-教师为主导"的教学模式兼顾传统"以教师为中心"教学模式与现代"以学生为中心"的教学模式优点,针对高职学生学习基础较差,学习积极..."SSTL"(student-subject and teacher-leader),即"学生为主体-教师为主导"的教学模式兼顾传统"以教师为中心"教学模式与现代"以学生为中心"的教学模式优点,针对高职学生学习基础较差,学习积极性、主动性不够等特点,利用教学敏感要素,提高高职课堂教学质量。展开更多
Background:Cervicitis is a common gynecological disease,which triggers local inflammation and tissue damage by a complex mechanism,seriously affecting women’s health.Chinese medicine plays a prominent role in the tre...Background:Cervicitis is a common gynecological disease,which triggers local inflammation and tissue damage by a complex mechanism,seriously affecting women’s health.Chinese medicine plays a prominent role in the treatment of gynecological inflammation.Shuangshi Tongling Capsule(SSTL),as a traditional Chinese medicine(TCM)compound,needs to be further elucidated for the treatment of cervicitis.Methods:Cervicitis was induced with 25%phenol gel(except controls),followed by 12-day intragastric treatment.Outcomes included genital inflammation scores,uterine index,and histopathology(HE staining).Serum tumor necrosis factor-α(TNF-α),Interleukin(IL)-6,IL-8,and IL-1βwere measured via ELISA.Integrated metabolomics-transcriptomics and Western blotting identified mechanistic pathways.Results:SSTL alleviated cervicitis in rats by improving uterine histopathology,reducing external genital inflammation scores(P<0.01 vs.model),and reversing uterine hypertrophy(P<0.05).Serum pro-inflammatory cytokines(TNF-α,IL-1β,IL-6,IL-8)were suppressed(P<0.01),with concurrent weight restoration.Multi-omics analysis identified AMPK signaling as pivotal,validated by downregulation of p-NF-κB(P<0.05)and upregulation of p-AMPK/SIRT1(P<0.05)in uterine tissues,indicating SSTL mitigates inflammation through AMPK/NF-κB/SIRT1 modulation.Conclusion:This study found that SSTL ameliorates cervicitis in rats through the expression of AMPK pathway-related proteins.展开更多
基金supported by Science and Technology Innovative Talent Program of Shaanxi University of Chinese Medicine(No.2024-CXTD-03)Key Research&Development Program of Shaanxi Provincial Department of Science and Technology(No.2024CY-JJQ-36,2024CY-JJQ-78)+4 种基金Shaanxi Provincial Administration of Traditional Chinese Medicine Research and Innovation Team Project(No.TZKN-CXTD-03)Youth Innovation Team of Aromatic Chinese Medicine Industrialization Key Technology(No.2022-95)Scientific Research Project of Shaanxi Administration of Traditional Chinese Medicine(ZYJXG-L23002)the Science and Technology Innovative Team Project of Shaanxi Administration of Traditional Chinese Medicine(No.2025-CXTD-02)The cartoon elements in the mechanism diagram are from https://www.figdraw.com.Shaanxi Provincial Science and Technology Department’s“two chains”integrated enterprise(institute)joint project(2023-LL-QY-39).
文摘Background:Cervicitis is a common gynecological disease,which triggers local inflammation and tissue damage by a complex mechanism,seriously affecting women’s health.Chinese medicine plays a prominent role in the treatment of gynecological inflammation.Shuangshi Tongling Capsule(SSTL),as a traditional Chinese medicine(TCM)compound,needs to be further elucidated for the treatment of cervicitis.Methods:Cervicitis was induced with 25%phenol gel(except controls),followed by 12-day intragastric treatment.Outcomes included genital inflammation scores,uterine index,and histopathology(HE staining).Serum tumor necrosis factor-α(TNF-α),Interleukin(IL)-6,IL-8,and IL-1βwere measured via ELISA.Integrated metabolomics-transcriptomics and Western blotting identified mechanistic pathways.Results:SSTL alleviated cervicitis in rats by improving uterine histopathology,reducing external genital inflammation scores(P<0.01 vs.model),and reversing uterine hypertrophy(P<0.05).Serum pro-inflammatory cytokines(TNF-α,IL-1β,IL-6,IL-8)were suppressed(P<0.01),with concurrent weight restoration.Multi-omics analysis identified AMPK signaling as pivotal,validated by downregulation of p-NF-κB(P<0.05)and upregulation of p-AMPK/SIRT1(P<0.05)in uterine tissues,indicating SSTL mitigates inflammation through AMPK/NF-κB/SIRT1 modulation.Conclusion:This study found that SSTL ameliorates cervicitis in rats through the expression of AMPK pathway-related proteins.
