Acute kidney injury(AKI)is a critical clinical condition characterized by rapid renal function decline,with high morbidity,mortality,and healthcare costs.Traditional Chinese medicine(TCM)has shown potential effects on...Acute kidney injury(AKI)is a critical clinical condition characterized by rapid renal function decline,with high morbidity,mortality,and healthcare costs.Traditional Chinese medicine(TCM)has shown potential effects on mitigating oxidative stress and programmed cell death in AKI models.Scutellaria barbata D.Don(SB)and Scleromitrion diffusum(Willd.)R.J.Wang(SD),a classic TCM herbal pair exhibited anti-inflammatory and antioxidant activities.Using advanced chromatographic separation technology,we enriched the effective fractions of water extracts from SB-SD,obtaining selfassembled herbal nanoparticles(SB and SD nanoparticles,SSNPs)rich in flavonoids and terpenoids.These SSNPs demonstrated robust antioxidant properties in vitro and mitigated AKI progression in vivo by activating the nuclear factor erythroid 2-related factor 2(Nrf2)signaling pathway.Oral administration of SSNPs in mice resulted in absorption into the bloodstream,formation of a protein corona,reduced macrophage phagocytosis,and enhanced bioavailability and renal targeting.Furthermore,we investigated the self-assembly principle of SSNPs using representative flavonoids and terpenoids.Kinetic studies and in situ transmission electron microscopy(in situ TEM)revealed that these compounds selfassemble via supramolecular forces like hydrogen bonding andπ-πinteractions,forming stable nanostructures.This study elucidates the renoprotective effects and mechanisms of SB and SD,and provides a novel approach for the development of TCM-based nanomedicines,highlighting the potential of nanoTCM in AKI treatment.展开更多
基金supported by the National Natural Science Foundation of China(Nos.81873195,32471458,and 82473933)the Applied Basic Research Program of Liaoning Province(No.2023JH2/101300103,China)+4 种基金the Liaoning Revitalization Talents Program(No.XLYC1907113,China)Chongqing Talents Program(cstc2022ycjh-bgzxm0111,China)the Natural Science Foundation of Chongqing,China(cstc2020jcyj-msxmX0411)Science and Technology Research Program of Chongqing Municipal Education Commission(KJZD-K202312802,China)the Distinguished Young Scholars in Dalian(2022RJ19,China).
文摘Acute kidney injury(AKI)is a critical clinical condition characterized by rapid renal function decline,with high morbidity,mortality,and healthcare costs.Traditional Chinese medicine(TCM)has shown potential effects on mitigating oxidative stress and programmed cell death in AKI models.Scutellaria barbata D.Don(SB)and Scleromitrion diffusum(Willd.)R.J.Wang(SD),a classic TCM herbal pair exhibited anti-inflammatory and antioxidant activities.Using advanced chromatographic separation technology,we enriched the effective fractions of water extracts from SB-SD,obtaining selfassembled herbal nanoparticles(SB and SD nanoparticles,SSNPs)rich in flavonoids and terpenoids.These SSNPs demonstrated robust antioxidant properties in vitro and mitigated AKI progression in vivo by activating the nuclear factor erythroid 2-related factor 2(Nrf2)signaling pathway.Oral administration of SSNPs in mice resulted in absorption into the bloodstream,formation of a protein corona,reduced macrophage phagocytosis,and enhanced bioavailability and renal targeting.Furthermore,we investigated the self-assembly principle of SSNPs using representative flavonoids and terpenoids.Kinetic studies and in situ transmission electron microscopy(in situ TEM)revealed that these compounds selfassemble via supramolecular forces like hydrogen bonding andπ-πinteractions,forming stable nanostructures.This study elucidates the renoprotective effects and mechanisms of SB and SD,and provides a novel approach for the development of TCM-based nanomedicines,highlighting the potential of nanoTCM in AKI treatment.
