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赤雹根总皂苷对类风湿性关节炎大鼠足跖组织中NF-κB p65活性作用的研究 被引量:7
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作者 顾士栋 阎昊 +1 位作者 佟继铭 曹凯 《时珍国医国药》 CAS CSCD 北大核心 2013年第8期1845-1847,共3页
目的研究赤雹根总皂苷(SRTD)对类风湿性关节炎大鼠足跖组织中NF-κB p65蛋白表达的影响,探讨SRTD治疗类风湿性关节炎的机制。方法雄性SD大鼠80只,随机取10大鼠作为正常对照,其余大鼠采用弗氏完全佐剂诱导佐剂性关节炎,将造摸成功的50只... 目的研究赤雹根总皂苷(SRTD)对类风湿性关节炎大鼠足跖组织中NF-κB p65蛋白表达的影响,探讨SRTD治疗类风湿性关节炎的机制。方法雄性SD大鼠80只,随机取10大鼠作为正常对照,其余大鼠采用弗氏完全佐剂诱导佐剂性关节炎,将造摸成功的50只大鼠随机分为模型组,赤雹根总皂苷160,80,40 mg/(kg.d)组和雷公藤多苷12 mg/(kg.d)组,各组分别给予相应药物,模型组ig蒸馏水,连续21 d。计算各组大鼠关节炎指数,给药后第22天,处死大鼠。用免疫组化法和Western blot法检测足跖组织中NF-κB p65蛋白的表达。结果赤雹根总皂苷能明显降低RA大鼠的关节炎指数(P<0.01,P<0.05),显著下调NF-κB p65蛋白的表达(P<0.01,P<0.05)。结论 SRTD对大鼠类风湿性关节炎有明显治疗作用,下调NF-κB p65蛋白的表达是其作用机制之一。 展开更多
关键词 赤雹根总皂苷 类风湿性关节炎 NF-ΚBP65
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一IFT172基因突变家系的临床表型及分子遗传学分析
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作者 杨赛赛 吴庆华 +4 位作者 陈心 史惠蓉 任淑敏 焦智慧 翟仪稳 《郑州大学学报(医学版)》 CAS 北大核心 2022年第4期542-546,共5页
目的:通过对一例以双肾及肝脏多发囊肿及全内脏反位等为主要症状的患者及其家系的临床表型及基因突变分析,揭示其遗传发病机制。方法:对该家系先证者行全面检查提示有多发异常,包括双肾及肝脏多发囊肿、全内脏反位、肾功能不全、肾性贫... 目的:通过对一例以双肾及肝脏多发囊肿及全内脏反位等为主要症状的患者及其家系的临床表型及基因突变分析,揭示其遗传发病机制。方法:对该家系先证者行全面检查提示有多发异常,包括双肾及肝脏多发囊肿、全内脏反位、肾功能不全、肾性贫血及甲状旁腺功能亢进等,采集先证者及其家系相关成员的外周血各2 mL,应用全外显子组测序,对筛查出的可疑突变行PCR并Sanger测序。结果:全外显子组测序及Sanger测序结果显示先证者携带IFT172基因c.4630C>T(p.R1544C)和c.4120T>C(p.W1374R)复合杂合突变,分别来自正常临床表型的父母,其弟未携带这两个突变。根据美国医学遗传学与基因组学学会指南,c.4120T>C(p.W1374R)判定为疑似致病性变异,包括2个中等证据(PM1+PM3)+3个辅助证据(PP3+PP4+PM2);c.4630C>T(p.R1544C)突变为致病性变异。结合该家系先证者临床表现和基因检测结果,明确先证者为IFT172基因复合杂合突变导致的短肋胸廓发育不良伴或不伴多指综合征10型(SRTD10)。结论:SRTD10临床表现复杂多样,根据临床表现难以确诊,基因诊断可以明确病因。 展开更多
关键词 多囊肾 多囊肝 全内脏反位 IFT172 srtd10
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Sensitive Resource and Traffic Density Risk Analysis of Marine Spill Accidents Using Automated Identification System Big Data 被引量:1
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作者 Eunlak Kim Hyungmin Cho +3 位作者 Namgyun Kim Eunjin Kim Jewan Ryu Heekyung Park 《Journal of Marine Science and Application》 CSCD 2020年第2期173-181,共9页
This study developed a new methodology for analyzing the risk level of marine spill accidents from two perspectives,namely,marine traffic density and sensitive resources.Through a case study conducted in Busan,South K... This study developed a new methodology for analyzing the risk level of marine spill accidents from two perspectives,namely,marine traffic density and sensitive resources.Through a case study conducted in Busan,South Korea,detailed procedures of the methodology were proposed and its scalability was confirmed.To analyze the risk from a more detailed and microscopic viewpoint,vessel routes as hazard sources were delineated on the basis of automated identification system(AIS)big data.The outliers and errors of AIS big data were removed using the density-based spatial clustering of applications with noise algorithm,and a marine traffic density map was evaluated by combining all of the gridded routes.Vulnerability of marine environment was identified on the basis of the sensitive resource map constructed by the Korea Coast Guard in a similar manner to the National Oceanic and Atmospheric Administration environmental sensitivity index approach.In this study,aquaculture sites,water intake facilities of power plants,and beach/resort areas were selected as representative indicators for each category.The vulnerability values of neighboring cells decreased according to the Euclidean distance from the resource cells.Two resulting maps were aggregated to construct a final sensitive resource and traffic density(SRTD)risk analysis map of the Busan–Ulsan sea areas.We confirmed the effectiveness of SRTD risk analysis by comparing it with the actual marine spill accident records.Results show that all of the marine spill accidents in 2018 occurred within 2 km of high-risk cells(level 6 and above).Thus,if accident management and monitoring capabilities are concentrated on high-risk cells,which account for only 6.45%of the total study area,then it is expected that it will be possible to cope with most marine spill accidents effectively. 展开更多
关键词 srtd risk analysis AIS big data Sensitive resource Marine spill accidents Marine traffic Traffic density Marine oil spill
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