A new SPME/HPLC interface is developed. It is based on thermal desorption from the SPME fiber and organic solvent collection of the desorbed analytes by sweeping them with an argon flow into a small organic solvent vo...A new SPME/HPLC interface is developed. It is based on thermal desorption from the SPME fiber and organic solvent collection of the desorbed analytes by sweeping them with an argon flow into a small organic solvent volume which is further injected into the HPLC chromatograph. Extraction and desorption parameters were investigated using five PAHs of different volatilities (naphthalene, acenaphthalene, fluorene, phenanthrene and anthracene) as test compounds and fluorimetric detection. Regression coefficients closed to 0.99 with RDS = 3) and detection limits in the range 0.07 - 0.99 μg.L-1 were found. A method was applied to determine the above PAHs in water samples. The results were compared with those supplied by the 550.1 EPA method showing the agreement of both methods at the 0.05 significance level.展开更多
The solid-phase microextraction（SPME）using C18-MCM-41 fiber coating coupling with high performance liquid chromatography（HPLC）was applied to the determination of trace of phenanthrene in environment water.The expe...The solid-phase microextraction（SPME）using C18-MCM-41 fiber coating coupling with high performance liquid chromatography（HPLC）was applied to the determination of trace of phenanthrene in environment water.The experimental conditions of SPME,.such as extraction time,extraction temperature,desorption time were optimized.The optimized conditions were as below:room temperature,1000r/min of stirring rate,30 min of extraction time and 3min of desorption time,respectively.The proposed method can be used to determine the phenanthrene in environmental water.展开更多
The composite mesoporous materials C2H5-MCM-41 are synthesized by multistep method,and its structure is characerized and evaluated.A solid-phase microextraction(SPME) method coupled to HPLC is proposed for the determi...The composite mesoporous materials C2H5-MCM-41 are synthesized by multistep method,and its structure is characerized and evaluated.A solid-phase microextraction(SPME) method coupled to HPLC is proposed for the determination of trace pyrene with C2H5-MCM-41 mesporous materials as the coating of extractor.Extraction time,extraction temperation,stirring rate,ionic strength,and desorption time had been optimized.The liner range is 0.15～150μg·L-1,detection limit is 0.062μg.L-1(S/N=3),whereas the RSD(n=5) range is 0.035%,which suggests that the sample pretreatment method of SPME is fast,convenient,recise,sensitive and solvent-free.展开更多
文摘A new SPME/HPLC interface is developed. It is based on thermal desorption from the SPME fiber and organic solvent collection of the desorbed analytes by sweeping them with an argon flow into a small organic solvent volume which is further injected into the HPLC chromatograph. Extraction and desorption parameters were investigated using five PAHs of different volatilities (naphthalene, acenaphthalene, fluorene, phenanthrene and anthracene) as test compounds and fluorimetric detection. Regression coefficients closed to 0.99 with RDS = 3) and detection limits in the range 0.07 - 0.99 μg.L-1 were found. A method was applied to determine the above PAHs in water samples. The results were compared with those supplied by the 550.1 EPA method showing the agreement of both methods at the 0.05 significance level.
文摘The solid-phase microextraction（SPME）using C18-MCM-41 fiber coating coupling with high performance liquid chromatography（HPLC）was applied to the determination of trace of phenanthrene in environment water.The experimental conditions of SPME,.such as extraction time,extraction temperature,desorption time were optimized.The optimized conditions were as below:room temperature,1000r/min of stirring rate,30 min of extraction time and 3min of desorption time,respectively.The proposed method can be used to determine the phenanthrene in environmental water.
文摘The composite mesoporous materials C2H5-MCM-41 are synthesized by multistep method,and its structure is characerized and evaluated.A solid-phase microextraction(SPME) method coupled to HPLC is proposed for the determination of trace pyrene with C2H5-MCM-41 mesporous materials as the coating of extractor.Extraction time,extraction temperation,stirring rate,ionic strength,and desorption time had been optimized.The liner range is 0.15～150μg·L-1,detection limit is 0.062μg.L-1(S/N=3),whereas the RSD(n=5) range is 0.035%,which suggests that the sample pretreatment method of SPME is fast,convenient,recise,sensitive and solvent-free.
