Insecticides are anthropogenic environmental stressors and also a common stressor for mosquito vectors.However,the use of insecticides is often guided by short-term efficacy,and the sublethal effect on their target or...Insecticides are anthropogenic environmental stressors and also a common stressor for mosquito vectors.However,the use of insecticides is often guided by short-term efficacy,and the sublethal effect on their target or nontarget species has long been ignored.Here,we analyzed how sublethal exposure of the promising vector-control bioinsecticide spinetoram to Aedes aegypti larvae alter adult performance and susceptibility to dengue virus(DENV)infection.We found that the surviving adult mosquitoes were significantly smaller and exhibited weaker blood-feeding capacity than control females,apart from the extended immature development period.In terms of reproductive potential,although the F_(0) generation produced a similar number of eggs and offspring during the first gonotrophic cycle,the survival rates of the F_(1) generations were significantly lower as compared to the control group,suggesting transgenerational sublethal effects on the F_(1) generation.Notably,surviving adult females had higher DENV-2 viral loads than the control group after spinetoram sublethal exposure.Mechanistically,transcriptomic analysis showed that inhibition of oxidative phosphorylation may function in stimulating DENV production in adult Ae.aegypti.In Aag2 cells,significant accumulation of apoptosis,mitochondrial reactive oxygen species production,and DENV-2 replication by spinetoram exposure consistently support our conclusion.Our study highlights the threat of sublethal spinetoram exposure on outbreaks of mosquito-borne viruses.展开更多
采用浸叶法并结合长沙、泰安、南昌等1年3地田间小区试验评价环丙氟虫胺等4种杀虫剂对甜菜夜蛾的室内活性和田间防效,为甜菜夜蛾的防控和抗药性治理提供数据支持。结果表明:环丙氟虫胺、溴虫氟苯双酰胺、氯虫苯甲酰胺、乙基多杀菌素对...采用浸叶法并结合长沙、泰安、南昌等1年3地田间小区试验评价环丙氟虫胺等4种杀虫剂对甜菜夜蛾的室内活性和田间防效,为甜菜夜蛾的防控和抗药性治理提供数据支持。结果表明:环丙氟虫胺、溴虫氟苯双酰胺、氯虫苯甲酰胺、乙基多杀菌素对甜菜夜蛾表现出较好的杀虫活性,其LC50值分别为0.0453、0.0411、0.0419、0.0425 mg/L:10%环丙氟虫胺可分散液剂(DC)在7.5、15、22.5 g a.i./hm^(2)剂量下,药后3、7 d防效达73.43%~100%,用量越高防效越优;100 g/L溴虫氟苯双酰胺悬浮剂(SC)在15 g a.i./hm^(2)剂量下药后3、7 d防效达87.48%~98.61%;200 g/L氯虫苯甲酰胺SC在30 g a.i./hm^(2)剂量下药后3、7 d防效达32.13%~72.01%;60 g/L乙基多杀菌素SC在36 g a.i./hm^(2)剂量下药后3、7 d防效达83.47%~99.76%。建议在氯虫苯甲酰胺抗性较高的区域优先推荐环丙氟虫按、溴虫氟苯双酰胺、乙基多杀菌素等防效优异的化学药剂,并选择不同作用机制的药剂轮替使用以延缓害虫抗药性的产生。展开更多
基金funded by the National Natural Science Foundation of China(82002166)the fellowship of China Postdoctoral Science Foundation(2020M672676)+3 种基金the Guangdong Project of Basic and Applied Basic Research(20201910240000150)funded by the National Natural Science Foundation of China(81902093)Guangzhou Science and Technology Project(202002030100)Funding from State Key Laboratory of Biocontrol(Sun Yat-sen University)(2020SKLBC-KF01).
文摘Insecticides are anthropogenic environmental stressors and also a common stressor for mosquito vectors.However,the use of insecticides is often guided by short-term efficacy,and the sublethal effect on their target or nontarget species has long been ignored.Here,we analyzed how sublethal exposure of the promising vector-control bioinsecticide spinetoram to Aedes aegypti larvae alter adult performance and susceptibility to dengue virus(DENV)infection.We found that the surviving adult mosquitoes were significantly smaller and exhibited weaker blood-feeding capacity than control females,apart from the extended immature development period.In terms of reproductive potential,although the F_(0) generation produced a similar number of eggs and offspring during the first gonotrophic cycle,the survival rates of the F_(1) generations were significantly lower as compared to the control group,suggesting transgenerational sublethal effects on the F_(1) generation.Notably,surviving adult females had higher DENV-2 viral loads than the control group after spinetoram sublethal exposure.Mechanistically,transcriptomic analysis showed that inhibition of oxidative phosphorylation may function in stimulating DENV production in adult Ae.aegypti.In Aag2 cells,significant accumulation of apoptosis,mitochondrial reactive oxygen species production,and DENV-2 replication by spinetoram exposure consistently support our conclusion.Our study highlights the threat of sublethal spinetoram exposure on outbreaks of mosquito-borne viruses.
文摘采用浸叶法并结合长沙、泰安、南昌等1年3地田间小区试验评价环丙氟虫胺等4种杀虫剂对甜菜夜蛾的室内活性和田间防效,为甜菜夜蛾的防控和抗药性治理提供数据支持。结果表明:环丙氟虫胺、溴虫氟苯双酰胺、氯虫苯甲酰胺、乙基多杀菌素对甜菜夜蛾表现出较好的杀虫活性,其LC50值分别为0.0453、0.0411、0.0419、0.0425 mg/L:10%环丙氟虫胺可分散液剂(DC)在7.5、15、22.5 g a.i./hm^(2)剂量下,药后3、7 d防效达73.43%~100%,用量越高防效越优;100 g/L溴虫氟苯双酰胺悬浮剂(SC)在15 g a.i./hm^(2)剂量下药后3、7 d防效达87.48%~98.61%;200 g/L氯虫苯甲酰胺SC在30 g a.i./hm^(2)剂量下药后3、7 d防效达32.13%~72.01%;60 g/L乙基多杀菌素SC在36 g a.i./hm^(2)剂量下药后3、7 d防效达83.47%~99.76%。建议在氯虫苯甲酰胺抗性较高的区域优先推荐环丙氟虫按、溴虫氟苯双酰胺、乙基多杀菌素等防效优异的化学药剂,并选择不同作用机制的药剂轮替使用以延缓害虫抗药性的产生。
