Objectives: To report three cases of gonorrhea withspectinomycin treatment failure, and to analyze thepossible causes.Methods: Identification of the three Neisseriagonorrhoeae isolates was made by an oxidase test anda...Objectives: To report three cases of gonorrhea withspectinomycin treatment failure, and to analyze thepossible causes.Methods: Identification of the three Neisseriagonorrhoeae isolates was made by an oxidase test anda rapid carbohydrate utilization test. The sensitivitytesting was performed by an agar dilution method.Results: All three isolates were identified as Ngonorrhoeae strains by positive oxidase test andbecause they only utilized glucose in the carbohydrateutilization test. All three strains were resistant tociprofloxacin and susceptible to ceftriaxoneSpectinomycin-resistant strains were found in onlytwo of the three patients, including one penicillinase-producing N. gonorrhoeae (PPNG). The third patienthad a spectinomycin sensitive strain, which was alsoPPNG and tetracycline resistant (TRNG). Based onthe medical history, spectinomycin treatment failurefor the third patient was due to inadequate injection.Conclusion: The causes of spectinomycin treatmentfailure of gonorrhea include spectinomycin resistanceand inadequate injection.展开更多
[Objectives] This study was conducted to establish a rapid detection method for spectinomycin in pork,chicken,fish,shrimp flesh and water.[Methods]A test strip for rapid detection of spectinomycin in milk was develope...[Objectives] This study was conducted to establish a rapid detection method for spectinomycin in pork,chicken,fish,shrimp flesh and water.[Methods]A test strip for rapid detection of spectinomycin in milk was developed by colloidal gold immunochromatography assay. [Results]The test strip had a detection limit of 50 μg/kg to milk with a detection time of 15 min,and the false positive rate and false negative rate were both 0. [Conclusions]The method is accurate,simple,reliable and convenient,and is suitable for rapid on-site spectinomycin detection.展开更多
A La^3＋-Cu/Pt modified electrode was fabricated by electrodepositing process in CuS04 solution by adding a small amount of lanthium compound, and it was employed for direct current （DC） amperometric detection of sp...A La^3＋-Cu/Pt modified electrode was fabricated by electrodepositing process in CuS04 solution by adding a small amount of lanthium compound, and it was employed for direct current （DC） amperometric detection of spectinomycin by anion-exchange chromatography. Without derivatization, this method can simultaneously determine the main component and impurities in spectinomycin pharmaceutical raw material. Ease of preparation, being applied in DC detection mode and good catalytic stability confirmed the interests of this modified electrode as amperometric sensor for the determination of spectinomycin.展开更多
A mathematical model for dilution crystallization of spectinomycin dihydrochloride pentahydrate was established on the basis of the population and mass balance. Three operating modes, i.e., constant mass rate addition...A mathematical model for dilution crystallization of spectinomycin dihydrochloride pentahydrate was established on the basis of the population and mass balance. Three operating modes, i.e., constant mass rate addition of diluent, constant mass fraction of diluent and constant size-independent growth rate, were investigated over a wide range of controlled parameters. The intrinsic characteristics of the crystallization process and the influence of operation parameters were analyzed in detail. Suitable strategy for better performance was suggested.展开更多
文摘Objectives: To report three cases of gonorrhea withspectinomycin treatment failure, and to analyze thepossible causes.Methods: Identification of the three Neisseriagonorrhoeae isolates was made by an oxidase test anda rapid carbohydrate utilization test. The sensitivitytesting was performed by an agar dilution method.Results: All three isolates were identified as Ngonorrhoeae strains by positive oxidase test andbecause they only utilized glucose in the carbohydrateutilization test. All three strains were resistant tociprofloxacin and susceptible to ceftriaxoneSpectinomycin-resistant strains were found in onlytwo of the three patients, including one penicillinase-producing N. gonorrhoeae (PPNG). The third patienthad a spectinomycin sensitive strain, which was alsoPPNG and tetracycline resistant (TRNG). Based onthe medical history, spectinomycin treatment failurefor the third patient was due to inadequate injection.Conclusion: The causes of spectinomycin treatmentfailure of gonorrhea include spectinomycin resistanceand inadequate injection.
基金Supported by Beijing Training Project for the Leading Talents in Science and Technology(Z171100001117158)
文摘[Objectives] This study was conducted to establish a rapid detection method for spectinomycin in pork,chicken,fish,shrimp flesh and water.[Methods]A test strip for rapid detection of spectinomycin in milk was developed by colloidal gold immunochromatography assay. [Results]The test strip had a detection limit of 50 μg/kg to milk with a detection time of 15 min,and the false positive rate and false negative rate were both 0. [Conclusions]The method is accurate,simple,reliable and convenient,and is suitable for rapid on-site spectinomycin detection.
基金supported by National Natural Science Foundation of China(No.20775070)Zhejiang Provincial Natural Science Foundation of China(No.Y407153)sponsored by Zhejiang Provincial Assay Foundation of China(No.2007F70017)
文摘A La^3＋-Cu/Pt modified electrode was fabricated by electrodepositing process in CuS04 solution by adding a small amount of lanthium compound, and it was employed for direct current （DC） amperometric detection of spectinomycin by anion-exchange chromatography. Without derivatization, this method can simultaneously determine the main component and impurities in spectinomycin pharmaceutical raw material. Ease of preparation, being applied in DC detection mode and good catalytic stability confirmed the interests of this modified electrode as amperometric sensor for the determination of spectinomycin.
文摘A mathematical model for dilution crystallization of spectinomycin dihydrochloride pentahydrate was established on the basis of the population and mass balance. Three operating modes, i.e., constant mass rate addition of diluent, constant mass fraction of diluent and constant size-independent growth rate, were investigated over a wide range of controlled parameters. The intrinsic characteristics of the crystallization process and the influence of operation parameters were analyzed in detail. Suitable strategy for better performance was suggested.
