Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. 7__X09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan ...Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. 7__X09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan a promising material for applications in coagulation, lubrication, protection against acute liver injury, and alleviating constipation. In this study, we cloned the Vitreosci/la hemoglobin gene into a broad-host-range plasmid pCM158. Without antibiotic selection, there was negligible loss of the plasmid in the host Agrobacterium sp. ZX09 after one passage of cultivation. The expression of Vitreoscilla hemoglobin was demonstrated by carbon monoxide （CO） dif- ference spectrum. The engineered strain Agrobacterium sp. ZX09 increased Salecan yield by 30%. The other physi- ological changes included its elevated respiration rate and cellular invertase activity.展开更多
基金supported by the Fundamental Research Funds for the Central Universities of China(No.30920130121013)the National Natural Science Foundation of China(No.31300111)
文摘Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. 7__X09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan a promising material for applications in coagulation, lubrication, protection against acute liver injury, and alleviating constipation. In this study, we cloned the Vitreosci/la hemoglobin gene into a broad-host-range plasmid pCM158. Without antibiotic selection, there was negligible loss of the plasmid in the host Agrobacterium sp. ZX09 after one passage of cultivation. The expression of Vitreoscilla hemoglobin was demonstrated by carbon monoxide （CO） dif- ference spectrum. The engineered strain Agrobacterium sp. ZX09 increased Salecan yield by 30%. The other physi- ological changes included its elevated respiration rate and cellular invertase activity.
