Objective: To study the expression of substance P receptor gene in the brain and marginal division in rats. Methods:In situ hybridiztion was performed on sections of the rat brain using digoxingenin--labeled substance...Objective: To study the expression of substance P receptor gene in the brain and marginal division in rats. Methods:In situ hybridiztion was performed on sections of the rat brain using digoxingenin--labeled substance P receptor (SPR ) cDNA as probes. Results: The positive reactive neurons distributed unevenly in the central nervous system. There were numerous strong SPR positive neurons in the cerebral cortex, hippocampus, amygdaloid nucleus and marginal division of the rat striatum. These results suggest that the marginal division cells of the striatum contain SPR mRNA and express SP receptor. Conclusion: It is supposed that the expression of SPR in marginal division of the striatum may play an important role in the reception and regulation of the neurotransmitter substance P, and the modulation of cholinergic activity involved in learning and memory of the marginal division.展开更多
目的观察比较眼针及体针对腹泻型肠易激综合征(D-IBS)大鼠下丘脑及结肠组织P物质(SP)及神经激肽Ⅰ型受体(NK-1)表达的影响。方法将40只雄性SD大鼠随机分为正常组、模型组、眼针组、体针组4组,每组10只。采用慢性应激结合束缚方法建立IB...目的观察比较眼针及体针对腹泻型肠易激综合征(D-IBS)大鼠下丘脑及结肠组织P物质(SP)及神经激肽Ⅰ型受体(NK-1)表达的影响。方法将40只雄性SD大鼠随机分为正常组、模型组、眼针组、体针组4组,每组10只。采用慢性应激结合束缚方法建立IBS大鼠模型。眼针组取下焦区、大肠区、肝区、脾区进行针刺,治疗7 d。用免疫组化方法检测大鼠下丘脑及结肠组织中SP和NK-1蛋白的表达;用RT-PCR方法检测大鼠下丘脑及结肠组织中SP m RNA的表达。结果与模型组比较,眼针组大鼠下丘脑和结肠组织SP和NK-1蛋白的表达以及SP m RNA的表达均明显减少(P<0.01)。结论眼针疗法优于体针,对D-IBS有治疗作用。展开更多
文摘Objective: To study the expression of substance P receptor gene in the brain and marginal division in rats. Methods:In situ hybridiztion was performed on sections of the rat brain using digoxingenin--labeled substance P receptor (SPR ) cDNA as probes. Results: The positive reactive neurons distributed unevenly in the central nervous system. There were numerous strong SPR positive neurons in the cerebral cortex, hippocampus, amygdaloid nucleus and marginal division of the rat striatum. These results suggest that the marginal division cells of the striatum contain SPR mRNA and express SP receptor. Conclusion: It is supposed that the expression of SPR in marginal division of the striatum may play an important role in the reception and regulation of the neurotransmitter substance P, and the modulation of cholinergic activity involved in learning and memory of the marginal division.
文摘目的观察比较眼针及体针对腹泻型肠易激综合征(D-IBS)大鼠下丘脑及结肠组织P物质(SP)及神经激肽Ⅰ型受体(NK-1)表达的影响。方法将40只雄性SD大鼠随机分为正常组、模型组、眼针组、体针组4组,每组10只。采用慢性应激结合束缚方法建立IBS大鼠模型。眼针组取下焦区、大肠区、肝区、脾区进行针刺,治疗7 d。用免疫组化方法检测大鼠下丘脑及结肠组织中SP和NK-1蛋白的表达;用RT-PCR方法检测大鼠下丘脑及结肠组织中SP m RNA的表达。结果与模型组比较,眼针组大鼠下丘脑和结肠组织SP和NK-1蛋白的表达以及SP m RNA的表达均明显减少(P<0.01)。结论眼针疗法优于体针,对D-IBS有治疗作用。
