目的:分析胃癌患者血清SOX9蛋白(SRY-related high mobility group-box 9,SOX9)、尾型同源盒转录因子(caudal related homeodomain transcription-2,CDX2)、胃蛋白酶水平变化及其与临床病理特征和预后的关系。方法:选取2021年1月至2024...目的:分析胃癌患者血清SOX9蛋白(SRY-related high mobility group-box 9,SOX9)、尾型同源盒转录因子(caudal related homeodomain transcription-2,CDX2)、胃蛋白酶水平变化及其与临床病理特征和预后的关系。方法:选取2021年1月至2024年1月于本院进行治疗的胃癌患者120例为胃癌组,另选择健康体检志愿者为健康组,采用酶联免疫吸附法检测血清中SOX9蛋白与胃蛋白酶水平;采用实时荧光定量PCR法检测血清中CDX2 mRNA表达量,比较两组受试者血清SOX9蛋白、CDX2、胃蛋白酶水平;并分析胃癌患者临床病理特征、预后与血清SOX9蛋白、CDX2、胃蛋白酶的关系。结果:胃癌组患者血清SOX9蛋白、胃蛋白酶水平高于健康组,血清CDX2 mRNA表达量低于健康组,(P<0.05)。胃癌患者血清SOX9蛋白、CDX2 mRNA表达量、胃蛋白酶均与临床TNM分期、是否淋巴结转移、是否侵入肌层、Lauren分型相关(P<0.05),血清SOX9蛋白、胃蛋白酶水平还与肿瘤分化程度有关(P<0.05)。Pearson相关性分析结果显示,胃癌患者无进展生存期、总生存期与血清SOX9蛋白、胃蛋白酶呈负相关,与血清CDX2呈正相关(P<0.05)。结论:胃癌患者血清SOX9蛋白、CDX2、胃蛋白酶水平与临床病理特征以及预后关系密切,可作为预后的重要参考指标。展开更多
Background:Human adipose-derived stem cells(hADSCs)are seed cells with application prospects in cartilage repair.However,the mechanism of hADSC chondrogenic differentiation is still unclear.This study identifies a nov...Background:Human adipose-derived stem cells(hADSCs)are seed cells with application prospects in cartilage repair.However,the mechanism of hADSC chondrogenic differentiation is still unclear.This study identifies a novel circRNA,circNR3C2,which is significantly upregulated during the chondrogenic differentiation of hADSCs.Methods:To analyze their role in hADSC chondrogenic differentiation,hADSCs were separated and identified by flow cytometry.Thereafter,we conducted Alcian Blue staining to assess chondrogenic differentiation levels.Additionally,RT-qPCR was carried out to detect levels of the cartilage-related genes COL2,Aggrecan and SOX9.Moreover,overlapping target SOX9 and circNR3C2 miRNAs were detected by bioinformatics and luciferase analyses.Finally,the role of circNR3C2 was confirmed in vivo using animal models.Results:We confirmed that the cell surface receptors CD44,CD90 and CD105 were positively expressed on hADSCs,and their cartilage differentiation levels dramatically increased after 2 weeks.Expression of the cartilage-related genes COL2 and Aggrecan and circNR3C2 also markedly increased.CircNR3C2 overexpression enhanced cartilage differentiation of hADSCs,while up-regulating COL2,SOX9 and Aggrecan.Bioinformatics analysis identified hsa-miR-647 as the target miRNA of circ-NR3C2 and SOX9.Hsa-miR-647 overexpression in hADSCs can antagonize the effect of circNR3C2 on chondrogenic differentiation,and reverse its effect on regulating the expression of COL2,Aggrecan,and SOX9.We also showed that hADSCs overexpressing circNR3C2 promote cartilage repair in vivo.Conclusions:We show that circNR3C2 modulates SOX9 expression to promote hsamiR-647-mediated hADSC chondrogenic differentiation;targeting circNR3C2 may help to develop new treatments to manage cartilage-related disorders.展开更多
基金supported by the Science and Technology Projects in Guangzhou(202201020566)Medical Joint Fund of Jinan University(YXJC2022005)+1 种基金Fundamental Research Funds for the Central Universities(21623319)Huadu District Basic and Applied Basic Research District and Hospital Joint Funding Program(23HDQYLH20).
文摘Background:Human adipose-derived stem cells(hADSCs)are seed cells with application prospects in cartilage repair.However,the mechanism of hADSC chondrogenic differentiation is still unclear.This study identifies a novel circRNA,circNR3C2,which is significantly upregulated during the chondrogenic differentiation of hADSCs.Methods:To analyze their role in hADSC chondrogenic differentiation,hADSCs were separated and identified by flow cytometry.Thereafter,we conducted Alcian Blue staining to assess chondrogenic differentiation levels.Additionally,RT-qPCR was carried out to detect levels of the cartilage-related genes COL2,Aggrecan and SOX9.Moreover,overlapping target SOX9 and circNR3C2 miRNAs were detected by bioinformatics and luciferase analyses.Finally,the role of circNR3C2 was confirmed in vivo using animal models.Results:We confirmed that the cell surface receptors CD44,CD90 and CD105 were positively expressed on hADSCs,and their cartilage differentiation levels dramatically increased after 2 weeks.Expression of the cartilage-related genes COL2 and Aggrecan and circNR3C2 also markedly increased.CircNR3C2 overexpression enhanced cartilage differentiation of hADSCs,while up-regulating COL2,SOX9 and Aggrecan.Bioinformatics analysis identified hsa-miR-647 as the target miRNA of circ-NR3C2 and SOX9.Hsa-miR-647 overexpression in hADSCs can antagonize the effect of circNR3C2 on chondrogenic differentiation,and reverse its effect on regulating the expression of COL2,Aggrecan,and SOX9.We also showed that hADSCs overexpressing circNR3C2 promote cartilage repair in vivo.Conclusions:We show that circNR3C2 modulates SOX9 expression to promote hsamiR-647-mediated hADSC chondrogenic differentiation;targeting circNR3C2 may help to develop new treatments to manage cartilage-related disorders.
