The Sox genes of P. dabryanus were amplified by degenerate primers within HMG-box. From male and female individuals, three amplification bands with the lengths 220, 550 and 1500 hp were displayed, respectively. A frag...The Sox genes of P. dabryanus were amplified by degenerate primers within HMG-box. From male and female individuals, three amplification bands with the lengths 220, 550 and 1500 hp were displayed, respectively. A fragment of potential novel gene was displayed in the band of 550 hp. It possibly coded 53 amino acid residues, whose amino acids had sequences in 96%, 94%, 90% and 47% similarities to those of the genes Sox 8, Sox 9, Sod o and SRY of mouse, and in 64%, 94%, 58% and 40% similarities to them of human, respectively. Accordingly, it was named PdSox 8. Northern blotting result indicated overexpression of it in adult liver and brain.展开更多
基金his project was supported by the National Doctorate Research Foundation of China the National Natural Science Foundation of
文摘The Sox genes of P. dabryanus were amplified by degenerate primers within HMG-box. From male and female individuals, three amplification bands with the lengths 220, 550 and 1500 hp were displayed, respectively. A fragment of potential novel gene was displayed in the band of 550 hp. It possibly coded 53 amino acid residues, whose amino acids had sequences in 96%, 94%, 90% and 47% similarities to those of the genes Sox 8, Sox 9, Sod o and SRY of mouse, and in 64%, 94%, 58% and 40% similarities to them of human, respectively. Accordingly, it was named PdSox 8. Northern blotting result indicated overexpression of it in adult liver and brain.
