BACKGROUND The prognosis for acute myeloid leukemia(AML)remains poor,underscoring the need for a deeper understanding of its underlying molecular mechanisms.AIM To assess the significance of SOX11 gene expression in t...BACKGROUND The prognosis for acute myeloid leukemia(AML)remains poor,underscoring the need for a deeper understanding of its underlying molecular mechanisms.AIM To assess the significance of SOX11 gene expression in the clinical features,response to treatment,and survival outcomes of adult patients with AML.METHODS This retrospective study enrolled 102 adults with AML.SOX11 gene expression in bone marrow samples was measured using real-time PCR.Data were correlated to the patients’clinical features,response to treatment,and survival rates.RESULTS Increased SOX11 expression was significantly associated with the presence of the FLT3-ITD mutation(P<0.001),the FAB-M2 subtype(P=0.008),and cytogenetic abnormalities(P=0.011).However,no significant association was found between SOX11 expression and other clinical laboratory parameters,complete remission,disease-free survival,or overall survival.CONCLUSION SOX11 expression may serve as a marker to identify specific subsets of AML patients who could benefit from intensive targeted chemotherapy.展开更多
Objective This study aimed to investigate the effect of Astragalus(AST)on osteoporosis(OP)and the downstream mechanisms.Methods Human bone marrow-derived mesenchymal stem cells(hBMSCs)were induced to differentiate int...Objective This study aimed to investigate the effect of Astragalus(AST)on osteoporosis(OP)and the downstream mechanisms.Methods Human bone marrow-derived mesenchymal stem cells(hBMSCs)were induced to differentiate into osteogenic cells.After transfection with relevant plasmids,cell proliferation,cell cycle progression,and apoptosis were assessed.Alizarin red staining was used to detect calcium nodules in the cells,alkaline phosphatase(ALP)staining was used to detect ALP activity in the cells,and quantitative reverse transcription-polymerase chain reaction and western blotting were used to determine RUNX2 and Osterix expression levels.An OP rat model was established using ovariectomy and micro-computed tomography scanning.Hematoxylin and eosin staining and Masson’s trichrome staining were used to evaluate the pathological conditions of bone tissues,while immunohistochemistry was conducted to detect RUNX2 in bone tissues.Results AST promoted the osteogenic differentiation of BMSCs,reduced miR-181d-5p expression levels,and increased SOX11 expression levels.Restoring miR-181d-5p expression or reducing SOX11 expression levels reversed the effects of AST on the osteogenic differentiation of hBMSCs.miR-181d-5p was found to target SOX11 in hBMSCs.AST improved OP in rats,and miR-181d-5p overexpression or SOX11 inhibition reversed the therapeutic effects of AST on OP in rats.Conclusion AST promoted the osteogenic differentiation of hBMSCs and alleviated OP by targeting SOX11 via miR-181d-5p.展开更多
Objective:SOX11 is expressed in numerous malignancies,including hepatocellular carcinomas(HCC),but its oncogenic function has not been elucidated.Here,we performed a comprehensive bioinformatics analysis of the Liver ...Objective:SOX11 is expressed in numerous malignancies,including hepatocellular carcinomas(HCC),but its oncogenic function has not been elucidated.Here,we performed a comprehensive bioinformatics analysis of the Liver Hepatocellular Carcinoma(LIHC)dataset to investigate the function of SOX11 in tumorgenesis.Methods:SOX11 expression data from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)were validated by immunohistochemistry(IHC).Co-expression,differential expression,and functional analyses utilized TCGA-LIHC,Timer 2.0,Metascape,GTEx,and LinkedOmics databases.Associations with immune infiltration,ferroptosis,and immune checkpoint genes were assessed.Genetic changes were explored via CBioPortal.Logistic regression,receiver operating characteristic curve(ROC),Kaplan-Meier analysis,and nomogram modeling evaluated associations with HCC clinicopathological features.SOX11’s impact on proliferation and migration was studied in HepG2 and HuH7 cell lines.Results:SOX11 was significantly elevated in HCC tumors compared to controls.SOX11-associated genes exhibited differential expression in pathways involving extracellular membrane ion channels.Significant associations were found between SOX11 levels,immune infiltration,ferroptosis,and immune checkpoint genes in HCC tissue.SOX11 levels correlated with HCC stage,histologic grade,and tumor status,and independently predicted overall and disease-specific survival.SOX11 expression effectively distinguished between tumor and normal liver tissue.Spearman correlations highlighted a significant relationship between SOX11 and ferroptosis-associated genes.Decreased SOX11 levels in HepG2 and HuH7 cells resulted in reduced proliferation and migration.Conclusions:SOX11 was found to represent a promising biomarker within HCC diagnosis and prognosis together with being a possible drug-target.展开更多
目的:探讨过表达SOX11(Sex determination region of Y chromosome 11)基因对人视网膜母细胞瘤Y79细胞增殖、凋亡的影响及参与该过程的细胞信号传到途径。方法:将用重组pc DNA3.1-SOX11质粒转染的Y79细胞作为实验组,以空载体pc-DNA3.1-m...目的:探讨过表达SOX11(Sex determination region of Y chromosome 11)基因对人视网膜母细胞瘤Y79细胞增殖、凋亡的影响及参与该过程的细胞信号传到途径。方法:将用重组pc DNA3.1-SOX11质粒转染的Y79细胞作为实验组,以空载体pc-DNA3.1-mock转染的细胞作为对照组。细胞增殖通过Edu细胞荧光染色法检测,细胞凋亡通过Hoechst 33342细胞染色法检测。PTEN、AKT及pAKT的表达采用westernblot法检测。结果:实验组中SOX11 mRNA的相对表达量显著高于对照组[(354±26.2)%vs(100±1.3)%,P<0.05]。实验组细胞在450nm吸光(OD)值低于对照组(2.8±0.3 vs4.5±0.3,P<0.05)。Edu免疫荧光染色结果证实,实验组细胞增殖比例较对照组低[(12.4±4.3)%vs(32.3±3.3)%,P<0.05]。Hoechst 33342细胞凋亡荧光染色结果表明,实验组细胞凋亡比例较对照组高[(10.3±2.3)%vs(5.3±1.4)%,P<0.05]。Western blot实验结果表明,实验组PTEN蛋白的相对表达量高于对照组(0.6±0.03 vs 0.3±0.03,P<0.05),磷酸化Akt的相对表达量低于对照组(0.4±0.02 vs 0.7±0.02,P<0.05)。结论:过表达SOX11基因显著抑制人视网膜母细胞Y79的增殖并促使细胞凋亡。PTEN/Akt信号通路的激活可能参与该过程。展开更多
文摘BACKGROUND The prognosis for acute myeloid leukemia(AML)remains poor,underscoring the need for a deeper understanding of its underlying molecular mechanisms.AIM To assess the significance of SOX11 gene expression in the clinical features,response to treatment,and survival outcomes of adult patients with AML.METHODS This retrospective study enrolled 102 adults with AML.SOX11 gene expression in bone marrow samples was measured using real-time PCR.Data were correlated to the patients’clinical features,response to treatment,and survival rates.RESULTS Increased SOX11 expression was significantly associated with the presence of the FLT3-ITD mutation(P<0.001),the FAB-M2 subtype(P=0.008),and cytogenetic abnormalities(P=0.011).However,no significant association was found between SOX11 expression and other clinical laboratory parameters,complete remission,disease-free survival,or overall survival.CONCLUSION SOX11 expression may serve as a marker to identify specific subsets of AML patients who could benefit from intensive targeted chemotherapy.
基金supported by the National Natural Science Foundation of China(No.81700888).
文摘Objective This study aimed to investigate the effect of Astragalus(AST)on osteoporosis(OP)and the downstream mechanisms.Methods Human bone marrow-derived mesenchymal stem cells(hBMSCs)were induced to differentiate into osteogenic cells.After transfection with relevant plasmids,cell proliferation,cell cycle progression,and apoptosis were assessed.Alizarin red staining was used to detect calcium nodules in the cells,alkaline phosphatase(ALP)staining was used to detect ALP activity in the cells,and quantitative reverse transcription-polymerase chain reaction and western blotting were used to determine RUNX2 and Osterix expression levels.An OP rat model was established using ovariectomy and micro-computed tomography scanning.Hematoxylin and eosin staining and Masson’s trichrome staining were used to evaluate the pathological conditions of bone tissues,while immunohistochemistry was conducted to detect RUNX2 in bone tissues.Results AST promoted the osteogenic differentiation of BMSCs,reduced miR-181d-5p expression levels,and increased SOX11 expression levels.Restoring miR-181d-5p expression or reducing SOX11 expression levels reversed the effects of AST on the osteogenic differentiation of hBMSCs.miR-181d-5p was found to target SOX11 in hBMSCs.AST improved OP in rats,and miR-181d-5p overexpression or SOX11 inhibition reversed the therapeutic effects of AST on OP in rats.Conclusion AST promoted the osteogenic differentiation of hBMSCs and alleviated OP by targeting SOX11 via miR-181d-5p.
基金supported by grants from Guizhou Nursing Vocational College Foundation(No.gzhlyj2023-04)Guizhou Nursing Vocational College Foundation(No.gzhlyj2021-02)+1 种基金Science and Technology Foundation of Guizhou Provincial Health Committee(No.gzwkj2022-518)Nature Science Foundation of Beijing,China(No.7214253).
文摘Objective:SOX11 is expressed in numerous malignancies,including hepatocellular carcinomas(HCC),but its oncogenic function has not been elucidated.Here,we performed a comprehensive bioinformatics analysis of the Liver Hepatocellular Carcinoma(LIHC)dataset to investigate the function of SOX11 in tumorgenesis.Methods:SOX11 expression data from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)were validated by immunohistochemistry(IHC).Co-expression,differential expression,and functional analyses utilized TCGA-LIHC,Timer 2.0,Metascape,GTEx,and LinkedOmics databases.Associations with immune infiltration,ferroptosis,and immune checkpoint genes were assessed.Genetic changes were explored via CBioPortal.Logistic regression,receiver operating characteristic curve(ROC),Kaplan-Meier analysis,and nomogram modeling evaluated associations with HCC clinicopathological features.SOX11’s impact on proliferation and migration was studied in HepG2 and HuH7 cell lines.Results:SOX11 was significantly elevated in HCC tumors compared to controls.SOX11-associated genes exhibited differential expression in pathways involving extracellular membrane ion channels.Significant associations were found between SOX11 levels,immune infiltration,ferroptosis,and immune checkpoint genes in HCC tissue.SOX11 levels correlated with HCC stage,histologic grade,and tumor status,and independently predicted overall and disease-specific survival.SOX11 expression effectively distinguished between tumor and normal liver tissue.Spearman correlations highlighted a significant relationship between SOX11 and ferroptosis-associated genes.Decreased SOX11 levels in HepG2 and HuH7 cells resulted in reduced proliferation and migration.Conclusions:SOX11 was found to represent a promising biomarker within HCC diagnosis and prognosis together with being a possible drug-target.
文摘目的:探讨过表达SOX11(Sex determination region of Y chromosome 11)基因对人视网膜母细胞瘤Y79细胞增殖、凋亡的影响及参与该过程的细胞信号传到途径。方法:将用重组pc DNA3.1-SOX11质粒转染的Y79细胞作为实验组,以空载体pc-DNA3.1-mock转染的细胞作为对照组。细胞增殖通过Edu细胞荧光染色法检测,细胞凋亡通过Hoechst 33342细胞染色法检测。PTEN、AKT及pAKT的表达采用westernblot法检测。结果:实验组中SOX11 mRNA的相对表达量显著高于对照组[(354±26.2)%vs(100±1.3)%,P<0.05]。实验组细胞在450nm吸光(OD)值低于对照组(2.8±0.3 vs4.5±0.3,P<0.05)。Edu免疫荧光染色结果证实,实验组细胞增殖比例较对照组低[(12.4±4.3)%vs(32.3±3.3)%,P<0.05]。Hoechst 33342细胞凋亡荧光染色结果表明,实验组细胞凋亡比例较对照组高[(10.3±2.3)%vs(5.3±1.4)%,P<0.05]。Western blot实验结果表明,实验组PTEN蛋白的相对表达量高于对照组(0.6±0.03 vs 0.3±0.03,P<0.05),磷酸化Akt的相对表达量低于对照组(0.4±0.02 vs 0.7±0.02,P<0.05)。结论:过表达SOX11基因显著抑制人视网膜母细胞Y79的增殖并促使细胞凋亡。PTEN/Akt信号通路的激活可能参与该过程。
