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Impact of sourdough fermentation on nutrient transformations in cereal-based foods:Mechanisms,practical applications,and health implications
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作者 Zhen Wang Luyang Wang 《Grain & Oil Science and Technology》 CAS 2024年第2期124-132,共9页
Sourdough is often considered a healthy choice and quality improver for use in cereal production due to its unique microbial composition and fermentation properties.During sourdough fermentation of cereals,biotransfor... Sourdough is often considered a healthy choice and quality improver for use in cereal production due to its unique microbial composition and fermentation properties.During sourdough fermentation of cereals,biotransformation of nutrients occurs,resulting in notable changes to proteins,carbohydrates,fats,vitamins,and minerals.Each nutrient undergoes specific transformations,providing various advantages for human health.Proteins undergo hydrolysis to produce small molecular weight peptides and amino acids that are more easily digested and absorbed by the human body.Carbohydrates break down to improve the digestibility and absorption of cereals and lower the glycemic index.Fatty acids experience oxidation to produce new substances with health benefits.Additionally,the application of sourdough fermentation can enhance the texture,flavor,and nutritional value of cereal foods while also extending their shelf life and improving food safety.In conclusion,sourdough fermentation has a broad range of applications in cereal food processing.Further research is encouraged to investigate the mechanisms and processes of sourdough fermentation to develop even more nutritious,healthy,and flavorful cereal-based foods. 展开更多
关键词 sourdough fermentation Lactic acid bacteria PROTEOLYSIS Starch hydrolysis Low-GI NUTRIENTS
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A study revealing the key aroma compounds of steamed bread made by Chinese traditional sourdough 被引量:9
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作者 Guo-hua ZHANG Tao WU +4 位作者 Faizan A.SADIQ Huan-yi YANG Tong-jie LIU Hui RUAN Guo-qing HE 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2016年第10期787-797,共11页
Aroma of Chinese steamed bread(CSB) is one of the important parameters that determines the overall quality attributes and consumer acceptance. However, the aroma profile of CSB still remains poorly understood, mainl... Aroma of Chinese steamed bread(CSB) is one of the important parameters that determines the overall quality attributes and consumer acceptance. However, the aroma profile of CSB still remains poorly understood, mainly because of relying on only a single method for aroma extraction in previous studies. Therefore, the objective of this study was to determine the volatile aroma compounds of five different samples of CSB using three different aroma extraction methods, namely solid-phase microextraction(SPME), simultaneous distillation–extraction(SDE), and purge and trap(PT). All samples showed a unique aroma profile, which could be attributed to their unique microbial consortia.(E)-2-Nonenal and(E,E)-2,4-decadienal were the most prevalent aromatic compounds revealed by SDE, which have not been reported previously, while ethanol and acetic acid proved to be the most dominant compounds by both SPME and PT. Our approach of combining three different aroma extraction methods provided better insights into the aroma profile of CSB, which had remained largely unknown in previous studies. 展开更多
关键词 Steamed bread sourdough Simultaneous distillation–extraction(SDE) Solid-phase microextraction(SPME) Purge and trap(P&T)
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Effects of Sourdough Addition on the Quality and Shelf Life of Chinese Steamed Bread 被引量:4
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作者 CHEN Di WANG Jinshui +1 位作者 JIA Feng ZHANG Changfu 《Grain & Oil Science and Technology》 2018年第2期85-90,共6页
The effect of adding sourdough with lactic acid bacteria(Lactobacillus plantarum M616) on dough quality and the sensory scores, specific volume, crumb firmness, and spread ratio of resultant Chinese steamed bread were... The effect of adding sourdough with lactic acid bacteria(Lactobacillus plantarum M616) on dough quality and the sensory scores, specific volume, crumb firmness, and spread ratio of resultant Chinese steamed bread were studied. The moisture content of crumb and texture characteristics of the Chinese steamed bread with sourdough during storage were analyzed. In this study, 10%,20%, 30%, and 40% of sourdough addition was investigated. The sourdough starter resulted in significant decrease in the water absorption, development time, stability, and farinograph quality number of the dough. The addition of sourdough improved the quality of Chinese steamed bread such as improving the sensory scores and specific volume, decreasing the crumb firmness, and a lower spread ratio was observed with an increase in the sourdough concentration up to 30%. Sourdough addition delayed a fast decrease in the moisture content of the Chinese steamed bread crumb during storage. The texture profile analysis of the bread crumb indicated that the addition of sourdough improved the quality and delayed the staling speed of Chinese steamed bread during storage.Moreover, inclusion of 30% sourdough produced the best protective effect on product staling. 展开更多
关键词 sourdough CHINESE STEAMED BREAD DOUGH QUALITY Shelf life Texture profile analysis
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Potential Application of Exopolysaccharides from Lactobacillus delbrueckii FASHADFF1 (LDYG2) and Weissella confusa FASHADFF1 (WCFF1) in Sourdough Bread Production 被引量:1
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作者 Racheal Oluwayemisi Fashogbon Jadesola Sanusi +2 位作者 Gbemisola Elizabeth Ogunleye Moyinoluwa Oreoluwa Akintunde Bukola Christianah Adebayo-Tayo 《Advances in Microbiology》 CAS 2022年第8期481-499,共19页
Baking of sourdough is a common practice and has the advantage of improving the nutritional value, sensory qualities and increasing the shelf life of the bread. This study therefore focus on the antimicrobial and anti... Baking of sourdough is a common practice and has the advantage of improving the nutritional value, sensory qualities and increasing the shelf life of the bread. This study therefore focus on the antimicrobial and antioxidant capacity of exopolysaccharides form Lactic Acid Bacteria (LAB) and its application in sourdough production. The Lactobacillus delbrueckii LDYG2 and Weissella confusa WCFF1 were collected from the culture collection Centre and the LABs were maintained in De Man, Rogosa and Sharpe (MRS) broth. Modified Exopolysaccharide Selection Medium (mESM) was used to produce the EPS while the total sugar concentration was determined using phenol-sulfuric acid method. The antibacterial, antioxidant, proximate, physical, organoleptic properties and the shelf life of the SDB produced were also evaluated. The quantity of EPS produced by LDYG2 and WCFF1 ranged from 4743.75 - 5090.03 g/L. Eight different sugars were present in both EPSLD and EPSWC with high antibacterial activity (24 mm and 23 mm) against B. cereus and S. aureus respectively. EPSLD and EPSWC had antioxidant capacity increased in a dose dependent (0.5 - 10 mg/mL) manner. EPSWCSDB had the highest proximate content except for moisture content. There was a significantly different (P ≤ 0.05) in the shelf life extension of the sourdough bread. WCEPSSDB was generally accepted in terms of colour, aroma, taste, texture and palatability. EPS produced by L. delbrueckii (EPSLD) and W. confusa (EPSWC) has antimicrobial and antioxidant capacity and can be used in production of nutraceutical sourdough bread with an improved shelf life and high consumer acceptability. 展开更多
关键词 Lactic Acid Bacteria EXOPOLYSACCHARIDE ANTIMICROBIAL Antioxidant sourdough Bread Shelf Life
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Effects of acetic acid bacteria in starter culture on the properties of sourdough and steamed bread 被引量:1
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作者 Haifeng Li Shuang Hu Jiake Fu 《Grain & Oil Science and Technology》 2022年第1期13-21,共9页
Sourdough starters for making traditional steamed bread are rich in microbes.Studies have shown that,in addition to yeast and lactic acid bacteria(LAB),acetic acid bacteria(AAB)are other functional species in sourdoug... Sourdough starters for making traditional steamed bread are rich in microbes.Studies have shown that,in addition to yeast and lactic acid bacteria(LAB),acetic acid bacteria(AAB)are other functional species in sourdough,but the influences of AAB on the properties of sourdough and steamed bread were rarely reported.This study aimed to assess the effects of a selected strain of acetic acid bacterium on the properties of sourdough and steamed bread.Sourdoughs and steamed breads were prepared from five different starter cultures,marked as CK(control check,with no starters),Y(only yeast),YL(yeast+LAB),YA(yeast+AAB)and YLA(yeast+LAB+AAB),and their properties were determined.The results of sour dough properties showed that the YLA sourdough had the highest total titratable acid of 14.6 mL and the lowest pH of 3.87 compared with the other groups after fermentation to 8 h;and the YLA sourdough had the greatest elasticity and viscosity.The results of quality properties of steamed bread revealed that the L*value(88.40±0.09)of the YA steamed bread was significantly higher than those of the YLA and YL steamed breads;the YLA steamed bread had the highest specific volume and the highest sensory score of 89.6±3.31 in comparison to those of the other groups;the hardness and chewiness of YLA and YA steamed breads were significantly lower than those of the other groups.Volatile compounds in the steamed breads were also determined by SPME-GC-MS.A total of 42 flavor substances were detected,including 3 kinds of alcohols,8 kinds of aldehydes,7 kinds of ketones,2 kinds of acids,15 kinds of esters,1 furan,and 6 kinds of other types of compounds,mainly alkanes and esters,followed by alcohols and aldehydes.The YLA steamed bread was richer in esters,aldehydes,and acids than those from the other treatment groups.By contrast,alkanes and aldehydes were dominant in the Y and YL steamed breads.Therefore,the flavor and quality of the steamed bread made from the YLA sourdough could be improved obviously.These results could provide references for the application of AAB in dough fermentation and the improvement of new kinds of starters. 展开更多
关键词 Acetic acid bacteria sourdough RHEOLOGY Steamed bread quality Volatile compounds
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旧金山果实乳杆菌生物学特性及其对发酵面食影响的研究进展
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作者 张国华 贺霞霞 +3 位作者 路婷 孙天 王灿烂 周世伟 《食品工业科技》 CAS 北大核心 2025年第1期431-438,共8页
旧金山果实乳杆菌(Fructilactobacillus sanfranciscensis)作为酸面团中常见的菌种,是具有良好应用价值的酸面团乳酸菌之一,该菌种通过自身的生长代谢及其与酸面团中酵母菌间的相互作用对发酵面食的风味、营养及质构等方面产生重要影响... 旧金山果实乳杆菌(Fructilactobacillus sanfranciscensis)作为酸面团中常见的菌种,是具有良好应用价值的酸面团乳酸菌之一,该菌种通过自身的生长代谢及其与酸面团中酵母菌间的相互作用对发酵面食的风味、营养及质构等方面产生重要影响。自1971年分离该菌株以来,其分类学地位经历多次变迁,命名从Lactobacillus sanfrancisco变更为Lactobacillus sanfranciscensis,在2020年确定为Fructilactobacillus sanfranciscensis,至今,该菌种相关的研究文献已达500余篇。目前,该菌种已列入欧洲食品安全局的可食用菌种名单和韩国的限制性使用的微生物菌种名单,但尚不在我国可食用菌种名单中。本文系统综述了旧金山果实乳杆菌目前的分类学地位及其在酸面团中的普遍性,菌种的生化特性及基因特性,并阐述了其对发酵面食的影响,探索旧金山果实乳杆菌在酸面团中的应用潜力,以期开发用于酸面团的标准化发酵剂,为传统发酵面食产业化提供理论基础。 展开更多
关键词 酸面团 旧金山果实乳杆菌 发酵面食 酿酒酵母 矮小哈萨克斯坦酵母菌
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老面融合酵母混合发酵制备荞麦南瓜馒头工艺研究
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作者 孙军涛 张智超 +3 位作者 鲁明园 张耀强 魏春刚 王志磊 《中国食品添加剂》 2025年第10期110-115,共6页
在保留老面风味的基础上,缩短面团发酵时间,研究荞麦南瓜老面馒头的生产工艺。以荞麦粉、南瓜粉和小麦粉为主要原料,采用老面发酵剂融合活性干酵母共同发酵,研究荞麦和南瓜复合粉添加量、面粉与老面复配比例、发酵温度、发酵时间和醒发... 在保留老面风味的基础上,缩短面团发酵时间,研究荞麦南瓜老面馒头的生产工艺。以荞麦粉、南瓜粉和小麦粉为主要原料,采用老面发酵剂融合活性干酵母共同发酵,研究荞麦和南瓜复合粉添加量、面粉与老面复配比例、发酵温度、发酵时间和醒发时间对荞麦南瓜老面馒头感官品质、比容和质构的影响;并对比分析不同发酵剂制备的荞麦南瓜馒头的面团发酵时间、感官品质、比容和质构。结果表明,荞麦南瓜老面馒头生产工艺为:荞麦粉和南瓜粉按照质量比1∶1制备的复合粉添加量为20%(以小麦面粉质量计),小麦面粉与老面的质量比为25∶20,发酵温度为35℃,发酵时间为50 min,醒发时间为30 min。与老面作为发酵剂相比,该生产工艺使面团的发酵时间缩短了15.6倍,与活性干酵母面团发酵时间相近,使荞麦南瓜馒头在保留老面风味的基础上,极大缩短面团发酵时间,为老面发酵面制品的工业化生产提供技术支持。 展开更多
关键词 馒头 荞麦 南瓜 老面 发酵时间
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不同酸面团发酵对麦麸膳食纤维馒头品质的影响
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作者 王晓 郑学玲 +3 位作者 王晓曦 孙冰华 李力 马森 《河南工业大学学报(自然科学版)》 北大核心 2025年第3期9-19,共11页
麦麸膳食纤维(wheat bran dietary fiber,WBDF)在强化面制品营养价值的同时会对其品质产生不利影响,为了改善富含WBDF面制品的品质,探究了不同酸面团(植物乳杆菌(LP)、自然发酵老面(LM))发酵对WBDF面团特性及其馒头品质的影响。结果表明... 麦麸膳食纤维(wheat bran dietary fiber,WBDF)在强化面制品营养价值的同时会对其品质产生不利影响,为了改善富含WBDF面制品的品质,探究了不同酸面团(植物乳杆菌(LP)、自然发酵老面(LM))发酵对WBDF面团特性及其馒头品质的影响。结果表明:相比于酿酒酵母和LP发酵,LM的面团内部微观结构更加均匀致密,具有较低的黏弹性;LM馒头的比容与对照组相比升高了29.28%,缓解了WBDF对馒头比容的负面影响;当WBDF添加量为15%时,LM发酵显著改善了WBDF馒头的硬度、胶黏性和咀嚼性,分别降低了50.12%、51.87%、54.20%;当WBDF添加量为15%时,LM馒头的总酚含量提高了1.61 mg/g,DPPH自由基清除能力和ABTS自由基清除能力分别提高了5.82、9.07百分点。酸面团发酵可有效改善WBDF馒头的比容和质构,提升抗氧化能力,且LM所制馒头的品质整体较好。 展开更多
关键词 酸面团 小麦麸皮 膳食纤维 馒头
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雪莲菌发酵乳制备老面发酵过程中微生物群落的变化
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作者 郑义鑫 张国华 +1 位作者 桑亚新 高洁 《中国食品学报》 北大核心 2025年第3期317-328,共12页
利用雪莲菌发酵乳制备老面,探究其传代过程中微生物多样性的变化。采用Illumina Miseq测序技术检测雪莲菌发酵乳和老面在传代过程中微生物群落组成及变化。结果表明:子囊菌门、未分类真菌、罗兹菌门、变形菌门、厚壁菌门、绿弯菌门为雪... 利用雪莲菌发酵乳制备老面,探究其传代过程中微生物多样性的变化。采用Illumina Miseq测序技术检测雪莲菌发酵乳和老面在传代过程中微生物群落组成及变化。结果表明:子囊菌门、未分类真菌、罗兹菌门、变形菌门、厚壁菌门、绿弯菌门为雪莲菌发酵乳和老面中的优势菌门,罗兹菌属、不动杆菌属为雪莲菌发酵乳和老面团中共同的优势菌属,片球菌属、酵母菌属为老面团中独有的优势菌属。老面传代4次仍能保持与前几代相同的微生物群落组成,在第6代时微生物多样性急剧下降,说明利用雪莲菌制备发酵乳能在老面中稳定传代4次,有潜力成为一种用于老面制作的发酵剂。本研究可为开发我国传统面制品发酵剂提供理论参考。 展开更多
关键词 雪莲菌发酵乳 老面 发酵剂 微生物多样性 传统发酵
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液态中种发酵法制作酸面团馒头及工艺参数的优化
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作者 田昊 赵仁勇 《河南工业大学学报(自然科学版)》 北大核心 2025年第4期1-9,共9页
为探究液态中种发酵法制作酸面团馒头的工艺,采用固态酸种制备液态酸种,通过测定液态酸种和中种中乳酸菌与酵母菌的活菌数、pH值与可滴定酸量(TTA量)等指标,确定液态酸种适合的成熟时间范围和连续培养工艺。在此基础上,研究中种发酵时... 为探究液态中种发酵法制作酸面团馒头的工艺,采用固态酸种制备液态酸种,通过测定液态酸种和中种中乳酸菌与酵母菌的活菌数、pH值与可滴定酸量(TTA量)等指标,确定液态酸种适合的成熟时间范围和连续培养工艺。在此基础上,研究中种发酵时间、酸种熟化时间、中种中小麦粉的添加量以及液态酸种的添加量等单因素对馒头品质的影响。以感官评分为响应值,通过响应面试验,优化制作酸面团馒头的工艺参数。对比分析了酵母快速法、冷藏中种法以及液态中种法等方法制作的馒头品质。结果表明:液态酸种适合的熟化时间为9~21 h;液态酸种自制备之日起,在连续培养4 d后,用其接种的液态中种中乳酸菌和酵母菌的活菌数、pH值与TTA量达到稳定状态;制作酸面团馒头的最佳工艺参数为中种发酵时间7 h、酸种熟化时间15 h、中种中小麦粉的添加量25%、液态酸种的添加量40%,在此条件下制作的馒头比容大,口感和风味好。液态中种发酵法可制作高品质的酸面团馒头,且液态酸种和中种可以通过管道输送,非常适合传统发酵剂馒头的工业化和规模化生产。 展开更多
关键词 液态酸种 液态中种 发酵 酸面团 馒头
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不同温度发酵的全麦酸面团质量评价及乳酸菌多样性分析和选育
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作者 王博 张维清 +3 位作者 高涵 王超 刘玉春 郭超 《食品工业科技》 北大核心 2025年第8期182-191,共10页
为探究发酵温度对全麦酸面团质量及乳酸菌多样性的影响,利用全长高通量测序技术系统解析不同温度发酵的全麦酸面团在成熟过程中细菌群落结构及其演替规律,通过理化分析评价酸面团质量,采用传统培养法分离优势菌种并从中筛选特异菌株。... 为探究发酵温度对全麦酸面团质量及乳酸菌多样性的影响,利用全长高通量测序技术系统解析不同温度发酵的全麦酸面团在成熟过程中细菌群落结构及其演替规律,通过理化分析评价酸面团质量,采用传统培养法分离优势菌种并从中筛选特异菌株。结果表明,发酵温度对酸面团的质量特征存在显著影响,随发酵温度升高,酸面团成熟时的pH从4.18降低到3.87,总滴定酸度(Total titratable acidity,TTA)从15.0 mL增加到24.0 mL,发酵温度≥30℃时,成熟酸面团总抗氧化活性、类黄酮和氨基酸含量显著增加(P<0.05),高温发酵可赋予全麦酸面团更好的质量特征。不同温度发酵成熟的全麦酸面团中优势乳酸菌存在显著物种差异,20℃和25℃以弯曲乳杆菌为主,30℃以弯曲乳杆菌和乳酸片球菌为主,37℃以桥粘液乳杆菌为主。通过酶活力筛选,乳酸菌(Lactic acid bacteria,LAB)LAB 31弯曲乳杆菌的纤维素酶活最高,为77.92±2.26 U/L,LAB 22乳酸片球菌的淀粉酶活最高为42.19±4.12 U/L。该研究为全麦发酵食品发酵剂的开发提供了一定的理论依据与菌种资源。 展开更多
关键词 发酵温度 全麦酸面团 乳酸菌 高通量测序技术 微生物多样性
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Comparison of the probiotic characteristics of Lactic Acid Bacteria (LAB) isolated from sourdough and infant feces 被引量:2
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作者 Yasemin Kaya Tayyibe Erten +3 位作者 Melike Vurmaz Hümeyra İspirli Ömer Şimşek Enes Dertli 《Food Bioscience》 SCIE 2022年第3期992-997,共6页
Probiotics are beneficial microorganisms with certain characteristics such as survival under gastrointestinal conditions,adhesion to intestinal surface and different functions which result in several health benefits t... Probiotics are beneficial microorganisms with certain characteristics such as survival under gastrointestinal conditions,adhesion to intestinal surface and different functions which result in several health benefits to host.With this regards,this study aimed to isolate Lactic Acid Bacteria (LAB) from sourdough and human feces as two different environments and to test their potential probiotic functions.In total nine distinct isolates were assessed with several probiotic functions such as in vitro adhesion to human colon cells,antimicrobial activities and survival under harsh gastrointestinal conditions.In general,distinct strains showed strong antimicrobial activity against the pathogenic and fungal strains tested in this study.Importantly,the adhesion levels to HT29 cells were determined between 0.29 % and 9.54% and Lactobacillus paracasei F7B showed the highest adhesion.All isolates tolerated the bile salt after 24 h and the infant feces isolates showed good survival characteristics at pH 4. 展开更多
关键词 Probiotic sourdough Infant feces ADHESION
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Amino acid, mineral, vitamin B contents and bioactivities of extracts of yeasts isolated from sourdough 被引量:1
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作者 Furkan Demirgul Omer Simsek Osman Sagdic 《Food Bioscience》 SCIE 2022年第6期355-362,共8页
Bread is one of the most consumed major leavened foods all around the world. Yeasts are responsible for leavening the bread dough. However, bread dough contains high amounts of yeast biomass that have the potential to... Bread is one of the most consumed major leavened foods all around the world. Yeasts are responsible for leavening the bread dough. However, bread dough contains high amounts of yeast biomass that have the potential to contribute to the bioavailability of the bread. This study aims to find out the potential effects of yeast biomass on bread bioavailability. For this purpose, yeast extracts were produced from three Saccharomyces cerevisiae , two Kazachstania humilis and one Torulaspora delbrueckii strains by autolysis and subsequently the nutritional compositions, phenolic compound profiles, antioxidant activities and immune modulation properties of the yeast extracts were determined. Among yeast samples, S . cerevisiae PFC401 showed high protein, essential amino acids, vitamin B7, total phenolic content and immune modulation property. However, K . humilis PFC393 stood out in terms of DPPH radical scavenging activity, and T . delbrueckii PFC394 in terms of major and minor minerals. These results indicated that the bioactive properties of yeast extracts vary depending on the yeast strain. In conclusion, results showed that yeasts not only leaven the bread dough but also contribute to the bioavailability of bread due to their rich nutritional compositions and bioactive properties. 展开更多
关键词 sourdough Yeast extract BIOAVAILABILITY Saccharomyces cerevisiae Kazachstania humilis Torulaspora delbrueckii
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Gluten-free flour fermented with autochthonous starters for sourdough production:Effect of the fermentation process 被引量:1
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作者 R.Lancetti E.Salvucci +2 位作者 M.Moiraghi G.T.Pérez L.S.Sciarini 《Food Bioscience》 SCIE 2022年第3期176-183,共8页
Refined rice flour and wholegrain quinoa and buckwheat flours were used to prepare gluten-free sourdoughs.Two autochthonous LAB strains were isolated,one from quinoa (Limosilactobacillus fermentum Q3) and the other fr... Refined rice flour and wholegrain quinoa and buckwheat flours were used to prepare gluten-free sourdoughs.Two autochthonous LAB strains were isolated,one from quinoa (Limosilactobacillus fermentum Q3) and the other from buckwheat (Lim.fermentum T5).Lactiplantibacillus plantarum ATCC8014 was used as a reference starter.Two fermentation processes were evaluated,a single-step process (30℃,24 h) named SD1,and a daily refreshment process (backslopping for 10 days) named SD2.Sourdoughs were freeze-dried prior to analyses.Pasting properties,pentosan and polyphenol contents,and antioxidant activity were analysed.Flour properties were significantly modified by fermentation.Polyphenol extractability increased during fermentation,which resulted in a higher antioxidant capacity,mainly through a reducing mechanism.Q3 and T5 strains produced the most remarkable modifications of flour properties.Differences were found between both fermentation processes,mainly as a result of the development of different microbiota.The single-step fermentation (SD1) showed to be a simple and appropriate method for improving flour properties. 展开更多
关键词 Rice QUINOA Buckwheat Wholegrain flours Spontaneous sourdough Autochthonous starter Antioxidant capacity Flour modification
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河南传统酸面团中酵母菌的筛选鉴定及其发酵性能的研究
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作者 李斌 杜帆帆 +3 位作者 袁嘉璐 黄维鹏 杨孝祖 李越 《中国酿造》 北大核心 2025年第4期139-143,共5页
为了获得传统酸面团专用优良酵母菌,采用传统培养分离方法从郑州、南阳、安阳、周口、商丘、三门峡6地采集的传统酸面团样品中分离纯化酵母菌,通过分子生物学技术对其进行菌种鉴定,并对其发酵性能进行研究。结果表明,分离纯化得到9株酵... 为了获得传统酸面团专用优良酵母菌,采用传统培养分离方法从郑州、南阳、安阳、周口、商丘、三门峡6地采集的传统酸面团样品中分离纯化酵母菌,通过分子生物学技术对其进行菌种鉴定,并对其发酵性能进行研究。结果表明,分离纯化得到9株酵母菌,经鉴定,1株为扣囊复膜酵母(Saccharomycopsis fibuligera),8株为酿酒酵母(Saccharomyces cerevisiae)。9株酵母菌的耐酸及耐乙醇能力不同,其中菌株Y2-1的耐受性较好,可耐受pH 1.5、乙醇体积分数为16%的环境。随着发酵时间的延长,添加不同酵母菌的酸面团的滴定酸度呈现上升趋势,pH值呈现下降趋势,面团体积逐渐增大,其中,酿酒酵母Y2-1发酵3 h时可以使面团的体积增加1.23倍。综上,酿酒酵母Y2-1的耐受性较好、发酵力最强,可作为优良菌株为后续直投式发酵剂的开发提供理论支持。 展开更多
关键词 酸面团 酵母菌 筛选 鉴定 耐受性 发酵力
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酸面团源酿酒酵母的发酵性能及其对精酿啤酒风味和品质的影响
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作者 卢玉 刘涛 +4 位作者 李慧 杨海莺 陈文波 牛兴和 赵凯 《中国酿造》 北大核心 2025年第7期195-202,共8页
以特种麦芽为原料,以商业酿酒酵母(Saccharomyces cerevisiae)Y71为对照,采用分离自我国传统酸面团中的2株酿酒酵母Y3和Y4酿造精酿啤酒,通过监测发酵过程中CO_(2)质量损失和酵母细胞数比较3种酿酒酵母的生长状况及发酵特性。采用常规检... 以特种麦芽为原料,以商业酿酒酵母(Saccharomyces cerevisiae)Y71为对照,采用分离自我国传统酸面团中的2株酿酒酵母Y3和Y4酿造精酿啤酒,通过监测发酵过程中CO_(2)质量损失和酵母细胞数比较3种酿酒酵母的生长状况及发酵特性。采用常规检测方法、顶空固相萃取(HS-SPME)结合气质联用(GC-MS)技术分析3种精酿啤酒理化指标及挥发性风味物质,通过相对气味活度值(ROAV)确定关键风味化合物,并进行主成分分析(PCA)及感官评价。结果表明,与酵母Y71相比,酵母Y3和Y4展现出了更优异的发酵特性和生长性能。酵母Y3和Y4的可发酵糖利用率分别为70.3%和70.0%,发酵度分别为77.06%和76.54%,酒精度分别为3.77%vol和3.74%vol。酵母Y3和Y4酿造精酿啤酒的各项理化指标均符合相关国家标准。HS-SPME-GC-MS结果显示,3种精酿啤酒中共检测出67种挥发性风味物质,基于ROAV>1共筛选出26种关键风味化合物,其中己酸乙酯、1-癸醇、椰子醛是酵母Y3、Y4酿造精酿啤酒中特有的香气物质。PCA能有效区分3种精酿啤酒样品,酵母Y3制备精酿啤酒风味协调,花香、果香突出,酒体协调、柔和。 展开更多
关键词 酸面团 酿酒酵母 精酿啤酒 发酵性能 挥发性风味物质 主成分分析
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Impact of starter culture on biochemical properties of sourdough bread related to composition and macronutrient digestibility
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作者 Jayani Kulathunga Kristin Whitney Senay Simsek 《Food Bioscience》 SCIE 2023年第3期1879-1888,共10页
Due to the desire for nutritionally dense foods,there is interest in whole grain and sourdough bread products.This research study was carried out to evaluate the effect of different starter cultures and stone mill set... Due to the desire for nutritionally dense foods,there is interest in whole grain and sourdough bread products.This research study was carried out to evaluate the effect of different starter cultures and stone mill settings on the nutritional attributes of sourdough bread.Using a stone mill,a blend of three hard red spring wheat varieties was ground into whole-grain flour.Three different gap settings and two different rotation speed values were used to produce flour samples with six different treatments,and those six flour samples were used to make sourdough bread with two different starter cultures.The experimental design was a randomized complete block design with a factorial arrangement.Significant differences(p<0.05)were observed in dietary fiber content,arabinoxylan content,total starch content,and protein digestibility between sourdough bread made with rye and wheat starter cultures.Higher protein digestibility values were observed for most of the sourdough samples with the rye starter culture compared to the wheat starter culture.In addition,the protein digestibility showed a strong negative correlation(r=-0.85)with the coarse particles and a positive correlation(r=0.70)with the fine particle size fraction.These results demonstrated that selected starter cultures and the raw material with the desired particle size could improve sourdough bread’s nutritional attributes and potential application in the baking industry. 展开更多
关键词 ARABINOXYLANS Dietary fiber DIGESTIBILITY Particle size sourdough Stone mill
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Effect of high-molecular weight dextran-enriched sourdough fermented using Leuconostoc mesenteroides ATCC 8293 on bread quality and gluten
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作者 Qing Bi Tingting Hong +2 位作者 Xing Mei Xueming Xu Dan Xu 《Food Bioscience》 SCIE 2023年第3期2663-2670,共8页
This study aimed to prepare high-molecular-weight(HMw)dextran-enriched sourdough(HMw-DT-SD)using Leuconostoc mesenteroides ATCC 8293 and to evaluate its effect on the quality of bread.The results demonstrated that HMw... This study aimed to prepare high-molecular-weight(HMw)dextran-enriched sourdough(HMw-DT-SD)using Leuconostoc mesenteroides ATCC 8293 and to evaluate its effect on the quality of bread.The results demonstrated that HMw-DT-SD improved the bread quality by increasing the bread volume from 3.86 cm^(3)/g to 5.04 cm^(3)/g;the bread staling rate decreased by 49.02%compared with that in control.The relationship between bread quality and dough gluten was further investigated.The acidity during bread making greatly influenced the effect of dextran on gluten.The HMw-DT-SD hindered the gluten depolymerization under acidic conditions(pH=3.6)and had a low free thiol content.In addition,the lower content of antiparallelβ-sheets in the bread dough might contribute to the depolymerization of macromolecular gluten(pH>4.4),thus improving the bread volume and texture.The HMw-DT-SD increased the content ofβ-turn structures in bread,suggesting gluten of lower resis-tance to extension. 展开更多
关键词 Bread GLUTEN High-molecular-weight dextran Leuconostoc mesenteroides sourdough
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Effects of freeze-dried pure strains to replace typeⅡsourdough in bread production
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作者 Yao Gu Xiaohu Luo +3 位作者 Haifeng Qian Yan Li Mingcong Fan Li Wang 《Food Bioscience》 SCIE 2023年第3期2281-2290,共10页
Type Ⅱ sourdough is the most popular application method in the industry,but it still has quality problems in bread production.This study evaluated the effect of replacing type Ⅱ sourdough with a new type of sourdoug... Type Ⅱ sourdough is the most popular application method in the industry,but it still has quality problems in bread production.This study evaluated the effect of replacing type Ⅱ sourdough with a new type of sourdough prepared from a pure freeze-dried strain(FDSS)on bread structure and flavor quality.It was found that 2-hep-tanone,2-undecanone,and 2-nonanone were the feature volatile compounds in type Ⅱ sourdough bread(SB).The variable importance in projection(VIP)scores of aroma-related compounds retained 72.23%,proving that FDSS retained SB’s aroma characteristics.The change rules of volatile compounds from freeze-dried strains were explored as the fermentation process proceeds.Analysis of texture quality revealed that proper acidity improves the quality of bread.All these results support the potential use of FDSS in the bread-making industry. 展开更多
关键词 sourdough bread FREEZE-DRIED Volatile compound TEXTURE
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传统酸面团中乳酸菌的筛选鉴定及耐受性研究
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作者 王梦珂 王猛 +6 位作者 康超 王婷 潘静 张坤 李璐 吴军林 方志刚 《农产品加工》 2025年第17期70-74,82,共6页
为从传统酸面团中筛选出耐逆境性良好的乳酸菌菌株,在MRS培养基上分离纯化、鉴定其中的乳酸菌,并进行耐受性分析,从酸面团中共分离纯化出2株耐受性较好的乳酸菌,经过形态学观察、生理生化鉴定和分子生物学鉴定分析,菌株R-GS1502鉴定为... 为从传统酸面团中筛选出耐逆境性良好的乳酸菌菌株,在MRS培养基上分离纯化、鉴定其中的乳酸菌,并进行耐受性分析,从酸面团中共分离纯化出2株耐受性较好的乳酸菌,经过形态学观察、生理生化鉴定和分子生物学鉴定分析,菌株R-GS1502鉴定为植物乳杆菌(Lactiplantibacillus argentoratensis),菌株R-HN21-0201鉴定为发酵乳杆菌(Limosilactobacillus fermentum),并测得2株菌的最适温度均为34℃,最适pH值均为5.5~6.5。耐受性结果表明,菌株R-HN21-0201对葡萄糖耐受性高于R-GS1502;耐酸试验中,R-HN21-0201的耐酸性高于R-GS1502,最高耐酸均为pH值3.0;耐乙醇和耐SO2试验中,R-GS1502的耐受性均高于R-HN21-0201。上述结果丰富了筛选耐受性良好乳酸菌的经验,为开发新型面团产品奠定了基础。 展开更多
关键词 酸面团 乳酸菌 分子生物学鉴定 耐受性
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