Estrogen mediates multiple functions in the brain through the interaction of estrogen receptor (ER)α and ERβ with a host of nuclear proteins that regulate specific gene transcription. We have identified ERAP 140, AI...Estrogen mediates multiple functions in the brain through the interaction of estrogen receptor (ER)α and ERβ with a host of nuclear proteins that regulate specific gene transcription. We have identified ERAP 140, AIB 1, Trk A, Src, pCREB and CREB as ERβ interacting proteins in the mouse brain. Earlier we showed that the interaction of ERβ with ERAP 140 decreased whereas its expression increased with aging in the brain of female mice. Here we report that the pattern of interaction and expression is different in male mice as compared to females. The interaction of ERAP 140 with ERβ decreased in adult male mouse brain as compared to young and remained almost similar in old whereas its expression was higher in adult than young and old, which were almost similar. Further in silico secondary structure analysis by self-optimized prediction method alignment (SOPMA) and PSIPRED revealed that ERβ interacting proteins were rich in alpha helices and coils. Such findings might help to design ER modulators which can regulate specific functions of estrogen in the brain during aging and degenerative diseases.展开更多
Objective:In the present study, the protein structure model of human amylin was generated to understand the protein's structure, function and mechanism of the action. Methods: The stereo chemical quality of the pr...Objective:In the present study, the protein structure model of human amylin was generated to understand the protein's structure, function and mechanism of the action. Methods: The stereo chemical quality of the protein model was checked by usingin silico analysis withSWISSMODEL,SOPMA,PROCHECK, ProSA andQMEAN servers. Results:The 66.7% residues in the core region of Ramachandran plot showing high accuracy of protein model and the QMEAN Z score of –6.57 indicates the overall model quality of amylin protein. Conclusions: These results may be helpful for further investigation on developing target molecules for amylin proteins that play a key role in type 2 diabetes.展开更多
文摘Estrogen mediates multiple functions in the brain through the interaction of estrogen receptor (ER)α and ERβ with a host of nuclear proteins that regulate specific gene transcription. We have identified ERAP 140, AIB 1, Trk A, Src, pCREB and CREB as ERβ interacting proteins in the mouse brain. Earlier we showed that the interaction of ERβ with ERAP 140 decreased whereas its expression increased with aging in the brain of female mice. Here we report that the pattern of interaction and expression is different in male mice as compared to females. The interaction of ERAP 140 with ERβ decreased in adult male mouse brain as compared to young and remained almost similar in old whereas its expression was higher in adult than young and old, which were almost similar. Further in silico secondary structure analysis by self-optimized prediction method alignment (SOPMA) and PSIPRED revealed that ERβ interacting proteins were rich in alpha helices and coils. Such findings might help to design ER modulators which can regulate specific functions of estrogen in the brain during aging and degenerative diseases.
文摘Objective:In the present study, the protein structure model of human amylin was generated to understand the protein's structure, function and mechanism of the action. Methods: The stereo chemical quality of the protein model was checked by usingin silico analysis withSWISSMODEL,SOPMA,PROCHECK, ProSA andQMEAN servers. Results:The 66.7% residues in the core region of Ramachandran plot showing high accuracy of protein model and the QMEAN Z score of –6.57 indicates the overall model quality of amylin protein. Conclusions: These results may be helpful for further investigation on developing target molecules for amylin proteins that play a key role in type 2 diabetes.
