[Objectives]To investigate the anti-hepatic fibrosis mechanism of lavandulyl flavonoid Kurarinol A(KA)from Sophora flavescens through the TGF/Smad signaling pathway.[Methods]A hepatic fibrosis model was established by...[Objectives]To investigate the anti-hepatic fibrosis mechanism of lavandulyl flavonoid Kurarinol A(KA)from Sophora flavescens through the TGF/Smad signaling pathway.[Methods]A hepatic fibrosis model was established by TGF-β1-induced activation of human hepatic stellate cells LX-2.Western blot and RT-qPCR techniques were employed to study the anti-fibrotic mechanism of KA through the TGF/Smad signaling pathway.[Results]KA exerted anti-hepatic fibrosis effects by significantly reducing the gene expression levels of TGF-β1,Smad2,Smad3,and Smad4,as well as markedly decreasing the protein expression levels of TGF-β1,p-Smad2/3/Smad2/3,and Smad4.[Conclusions]KA demonstrates significant anti-hepatic fibrosis activity and alleviates liver fibrosis through the TGF/Smad signaling pathway.展开更多
Objective:To assess the efficiency of a Sophora flavescens Ait(S.flavescens,Ku Shen)-soluble microneedle(SFA-MN)for improving skin lesion symptoms in mice with psoriasis.Methods:SFA-MNs were prepared using a two-mold ...Objective:To assess the efficiency of a Sophora flavescens Ait(S.flavescens,Ku Shen)-soluble microneedle(SFA-MN)for improving skin lesion symptoms in mice with psoriasis.Methods:SFA-MNs were prepared using a two-mold molding process with 20%w/v poly-vinylpyrrolidone and 15%w/v polyvinyl alcohol.The SFA-MNs were assessed for morphology,mechanical properties,in vitro dissolution,identification of components,and skin lesion improvement in imiquimod-induced psoriasis mice.Results:The SFA-MNs demonstrated good mechanical properties for efficiently penetrating the dermis,facilitating efficient drug delivery.Furthermore,they effectively inhibited mast cell levels in the dorsal lesion area of psoriasis mice and reduced the expression of the T-lymphocyte factor cluster of differ-entiation 3 and tumor necrosis factor-a.In addition,this system alleviated skin inflammation,splenic swelling,and thymic atrophy in the psoriasis-like mouse model.Seven major components were detected from SFA-MNs by comparison of the mass-to-nucleus ratios(m/z)of the secondary fragments N-methylcytisine,5a,9a-dihydroxymatrine,sophoramine,matrine,oxysophocarpine,oxymatrine,and kushenol O.Conclusion:The drug delivery strategy combining traditional herbal S.flavescens with soluble micro-needle technology provides more targeted and effective immune regulation for treating psoriasis-like mice models,enabling enhanced therapeutic effects compared with the control group.展开更多
In the context of a surging demand for functional foods,this study utilized Sophora japonica L.(SL)and Rosa rugosa Thunb.(RT),which are rich in polyphenols(with flavonoids as the core subclass).High-purity extracts(SL...In the context of a surging demand for functional foods,this study utilized Sophora japonica L.(SL)and Rosa rugosa Thunb.(RT),which are rich in polyphenols(with flavonoids as the core subclass).High-purity extracts(SLE and RTE)were obtained through ethanol reflux extraction and macroporous resin purification,and then formulated with maltodextrin and erythritol to prepare a composite solid beverage.This beverage exhibited excellent antioxidant capabilities.At a concentration of 1 mg/mL,the scavenging rates of DPPH,ABTS,and hydroxyl radicals reached 82.4%,94.6%,and 49.2%,respectively.Network pharmacology indicated that quercetin andβ-sitosterol could modulate lipid metabolism pathways.Moreover,the beverage showed potential for lipid-lowering.Its cholate adsorption capacity was 589.4±2.9 mg/g at pH 7.0,and the IC50 value for pancreatic lipase inhibition was 32.55 mg/mL.However,a 60-day storage stability test revealed that the moisture content approached 5%,likely due to polyphenol-flavonoid reactivity,extending dissolution time to 30.88 s.These changes were attributed to polyphenols(with flavonoids as the core active subclass,and non-flavonoids such as phenolic acids as auxiliary),resulting in color alterations and reduced solubility.This study confirmed the dual functions of the SL-RT beverage in antioxidant and lipid-lowering aspects.Nevertheless,it also pointed out the need to optimize the formula and process to enhance stability,providing an important basis for the development of stable functional beverages.展开更多
Background:Sophorae Tonkinensis Radix et Rhizoma(ST,also known as ShanDouGen)pertains to the Sophora genus,which is generally distributed in Southwest China.As a celebrated folk medicine,it has heat-clearing and detox...Background:Sophorae Tonkinensis Radix et Rhizoma(ST,also known as ShanDouGen)pertains to the Sophora genus,which is generally distributed in Southwest China.As a celebrated folk medicine,it has heat-clearing and detoxifying,reducing swelling and soothing pharynx.In recent years,there has been a notable rise in adverse events,including hepatotoxicity,associated with the use of ST,however,the mechanism behind ST-induced hepatotoxicity is unclear.Methods:The effects of ST on liver injury were investigated in vivo.Then,serum and liver samples were then analyzed using high-throughput metabolomics techniques.Furthermore,the application of network pharmacology and multivariate statistical analysis has been instrumental in identifying and predicting biomarkers and targets linked to liver toxicity.Results:In this study,we found that ST extract has a certain degree of damage to the rat liver.Using UPLC-MS/MS techniques,463 compounds were identified from ST,of which 73 compounds were absorbed in the blood of the STH group.Metabolomics results showed that amino acid metabolic biomarkers were associated with liver toxicity induced by ST.Notably,we identified 22 core ingredient as toxic effects Q-markers of ST through correlation analysis between biomarkers and absorbed components.On this basis,it is concluded by network pharmacology that ST may cause liver toxicity through PI3K-Akt signaling pathway,NF-kappa B signaling pathway,MAPK signaling pathway,and other pathways.Conclusion:Our findings also demonstrated that the“chemical composition-blood migration component-liver metabolism”stactics has an enormous potential to discern biomarkers and ingredients,and to elaborate the complexity toxicity mechanism of ST.展开更多
A lectin protein(SFL) with molecular weight about 32 kD which markedly agglutinated rabbit and human red blood cells was purified from the roots of Sophora flavescens Ait. This protein, and apparently inhibited the gr...A lectin protein(SFL) with molecular weight about 32 kD which markedly agglutinated rabbit and human red blood cells was purified from the roots of Sophora flavescens Ait. This protein, and apparently inhibited the growth of Fusarium vasinfectum Atk., Gibberella saubinetii (Mont.) Sacc., and Piricularia oryzae Cav. A set of degenerate PCR primer was synthesized according to the N-terminal sequence of the purified protein. The full-length cDNA coding the lectin was cloned by RT-PCR and 5'-RACE and sequenced (GenBank AF285121). The deduced amino acid sequence indicates that a preprotein with 284 amino acid residues is firstly translated and then processed to a mature protein with 254 amino acids. A N-Glycosylation site is the Asn 182 residue.展开更多
[Objective] We aimed to investigate the preventive and therapeutical effect of compound of traditional Chinese drugs （Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root） on acute alc...[Objective] We aimed to investigate the preventive and therapeutical effect of compound of traditional Chinese drugs （Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root） on acute alcohol intoxication in mice. [Method] Acute alcohol intoxication was induced by administering alcohol to mice. Three different doses （low, middle and high） of compound of traditional Chinese drugs were administered to mice before and after administering alcohol respectively to investigate the preventive and therapeutical effect of drugs on acute alcohol intox-ication through doing statistical analysis about drunk mice and their sleeping time. The concentration of malondialdehyde （MDA）, reduced glutathione （GSH） and triglyc-erides （TG） in liver was also determined to investigate the protective effect of drugs on liver. [Result] The efficacy of compound of traditional Chinese drugs on acute al-cohol intoxication was dose-dependent. High-dose administration decreased the number of drunk mice significantly compared with control group; middle- and high-dose administration reduced the sleeping time of drunk mice and the concentration of MDA and TG in liver tissue; three doses al increased the concentration of GSH. [Conclusion] The compound of Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root had preventive and therapeutical effect on hangover, and it also had certain preventive and therapeutical effect on liver damage caused by alcohol.展开更多
Flos Sophorae and Fructus Sophorae are two kinds of traditional Chinese medicines. In this work, the two kinds of traditional Chinese medicines collected from eleven areas of Dezhou, were analyzed by inductively coupl...Flos Sophorae and Fructus Sophorae are two kinds of traditional Chinese medicines. In this work, the two kinds of traditional Chinese medicines collected from eleven areas of Dezhou, were analyzed by inductively couple plasma-mass spectrometry （ICP-MS） to compare the content and distribution of 14 kinds of rare earth elements （REEs）. The method was verified by analyzing GBW07605 certified reference material. The results showed that ICP-MS is an accurate, sensitive and reliable technique for determining REEs in traditional Chinese medicine. There were big differences in contents for REEs in Flos Sophorae and Fmctus Sophorae from different areas. The contents of total REEs in Flos Sophorae samples from different areas ranged from 1.0785 to 2.2659 μg/g, while those in Fmctus Sophorae from 0.6826 to 1.0527 ktg/g. The contents of total REEs in Flos Sophorae samples from different areas were obviously higher than those in Fmctus Sophorae of the same area and there was big difference between various Flos Sophorae samples. Interestingly, the higher the content of total REEs in Flos Sophorae samples, the lower the content of total REEs in Fmctus Sophorae samples of the same area. The plots of normalized element concentration versus atomic number showed some characteristic distribution trends. The distribution trend of light REEs （La-Gd） was relatively fiat except a positive Eu anomaly, however, that was steep and discrepant for heavy REEs （Tb-Lu）. The results could provide a valuable reference for understanding the relationship between the curative mechanism, pharmacology characteristics and their geological condition for the two traditional Chinese medicines investigated.展开更多
Sophorae Flavescentis Radix(Sophora flavescens Ait., SFR) and Sophorae Tonkinensis Radix et Rhizoma(S. tonkinensis Gapnep., STR) are two commonly used traditional Chinese medicines from Sophora(Leguminosae) plants, wh...Sophorae Flavescentis Radix(Sophora flavescens Ait., SFR) and Sophorae Tonkinensis Radix et Rhizoma(S. tonkinensis Gapnep., STR) are two commonly used traditional Chinese medicines from Sophora(Leguminosae) plants, which are believed to possess similar bioactive components with entirely different clinical applications. In order to find out the characteristic chemical constituents potentially leading to the unique medicinal properties claimed for each of the two closely related TCMs, an HPLC fingerprint method was developed for analyses of the alkaloid and flavonoid constituents of SFR and STR, respectively, which were further evaluated and compared through similarity calculation and hierarchical clustering analysis(HCA). The results from the present study showed that the alkaloid fingerprints of the two herbs were similar, with many components co-existing in both drugs and various batches of samples from different species being mixed together in the HCA dendrogram. However, their flavonoid constituents were totally different with specific fingerprints being yielded for each herb, and further HCA analysis showed that the tested samples could almost be clearly divided into two groups based on their origins of species. The results from the present study indicated that the flavonoid constituents could serve as the differentially diagnostic constituents of SFR and STR and might potentially attributed to their distinct therapeutic effects.展开更多
[ Objective ] The paper was to study and analyze the genetic diversity of 48 endophytic bacteria isolated from Sophora alopecuroide with strong antagonis- tic effect against Fusarium oxysporum and Verticillium dahliae...[ Objective ] The paper was to study and analyze the genetic diversity of 48 endophytic bacteria isolated from Sophora alopecuroide with strong antagonis- tic effect against Fusarium oxysporum and Verticillium dahliae, and carry out sequence measurement and phylogenetic analysis on 10 representative strains. [ Meth- od] Using total DNA ERIC-PCR and 16S rRNA sequencing analysis method, a total of 48 endophytic bacteria were carried out genetic diversity and phylogenetic analysis. [Result] ERIC-PCR fingerprint map showed that 48 endophytic bacteria were divided into 6 ERIC groups and 2 strains with independent groups at Wat- son distance of 0.31. Representative strains were selected from each group to determine 16S rRNA gene for phylogenetic analysis, and the results showed that these strains belonged to Bacillus atrophaeus, Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Bacillus pumilus and Serratia marcescens, respectively. [ Conclu- sion ] The genetic diversity of 48 strains of bacteria was obvious. Determination of 48 strains of bacteria on diversity and phylogenetic status could lay foundation for study on mechanism of F. oxysporum and V. dahliae, which could also provide new strain sources for biological control of diseases in cotton production.展开更多
[Objective] The research aimed to discuss the inhibitory effect of Sophora japonica extracts against the growth of Microcystis aeruginosa.[Method] The inhibitory effect of extracted liquid of Sophora japonica leaf aga...[Objective] The research aimed to discuss the inhibitory effect of Sophora japonica extracts against the growth of Microcystis aeruginosa.[Method] The inhibitory effect of extracted liquid of Sophora japonica leaf against the growth of M.aeruginosa was measured.Moreover,the active component was studied and analyzed initially.[Result] The absolute alcohol extract of Sophora japonica leaf was separated by n-hexane,ethyl acetate,n-butanol and water phases in turn.The polar fractions were found being the majority （〉60%）.The non-polar fraction in n-nexane （about 25%） was found significantly inhibiting the growth of M.aeruginosa.The inhibition rates of fraction in n-hexane at the concentrations of 25 and 50 mg/L against M.aeruginosa in 7 d were higher than 75% and 90% respectively.In addition,chlorophyll a of M.aeruginosa was also destroyed in the presence of the hexane fraction.[Conclusion] The research provided the theoretical basis for preventing and controlling the water bloom of M.aeruginosa.展开更多
The method of cultivating seedlings of Sophora japonica f.flavi-rameus on rootstocks of Sophora japonica Linn.was introduced in this study,including rootstock cultivation,grafting and post-grafting management,specific...The method of cultivating seedlings of Sophora japonica f.flavi-rameus on rootstocks of Sophora japonica Linn.was introduced in this study,including rootstock cultivation,grafting and post-grafting management,specifically,seed collection,sowing,and breeding large seedlings in the cultivation of rootstocks;grafting time,scion collection and treatment,rootstock treatment,as well as techniques of stump grafting and bud grafting;focuses of post-grafting management,such as checking the survival conditions and untying the film,bud picking,pinching,topiary work,moisture and fertilization management,disease and pest control.Then the application of Sophora japonica f.flavi-rameus in gardens was analyzed,and it was proposed that biological characteristics and aesthetic principles should be followed,proper planting patterns should be applied,such as isolated planting,group planting,mass planting,linear planting and so on,moreover,it could also be combined with other species to create outstanding landscape effects.展开更多
Three novel matrine-type alkaloids(1-3)and two unprecedented aloperine-type alkaloids(4 and 5)were isolated from the root of Sophora tonkinensis and the seeds of Sophora alopecuroides respectively.Notably,compound 1 p...Three novel matrine-type alkaloids(1-3)and two unprecedented aloperine-type alkaloids(4 and 5)were isolated from the root of Sophora tonkinensis and the seeds of Sophora alopecuroides respectively.Notably,compound 1 possessed an unprecedented 6/5/6 tricyclic skeleton,while compounds 2 and 3 characterized by rare 6/6/5/6 tetracyclic system and 6/6/6/6/6 pentacyclic system respectively.Moreover,compound 4 possessed an unprecedented 6/7/6/6 tetracyclic core,and compound 5 characterized by rare 6/6/6/6tetracyclic skeleton.Their structures were elucidated by comprehensive spectroscopic data analysis and electronic circular dichroism(ECD)calculations.Biological tests indicated that compound 5 displayed significant anti-tobacco mosaic virus(TMV)activity compared with the positive control ningnanmycin.展开更多
Sophora moorcroftiana(S. moorcroftiana) is an endemic leguminous dwarf shrub in Tibet, China. Decoctions of the seeds have been used in Chinese folk medicine for dephlogistication, detoxication, and infectious disease...Sophora moorcroftiana(S. moorcroftiana) is an endemic leguminous dwarf shrub in Tibet, China. Decoctions of the seeds have been used in Chinese folk medicine for dephlogistication, detoxication, and infectious diseases. The present study aimed to investigate the constituent and biological effects of polysaccharides from S. moorcroftiana seeds in Caenorhabditis elegans(C. elegans). Polysaccharides from S. moorcroftiana seeds(SMpol) were extracted with 60% ethanol and constituent was analyzed by GC-MS. SMpol was composed of glucose, galactose and inositol in the molar ratio of 35.7 : 1.3 : 17.0. Synchronized worms were treated with SMpol and then lifespan, motility, reproduction, stress resistance and antimicrobial activity were examined. Compared with the control group, the lifespan was increased to the average of 27.3 days and the number of laying eggs showed a 1.3-fold increase in nematodes treated with SMpol(4 mg×mL^(–1)). In SMpol(4 mg×mL^(–1)) treated worms, there was a 1.1-fold increase in 24-h survival of acute heat stress and a 1.6-fold increase in 2-h survival of oxidative stress The colonization of the bacteria in the SMpol treated nematode was significantly lower than that of the untreated group by 68.3%. In vivo studies showed SMpol significantly extended the life span, improved reproduction, increased stress resistance and antimicrobial capacity of C. elegans. In conclusion, those results indicated that the polysaccharides from S. moorcroftiana seeds were involved in a variety of biological activities leading to its modulatory effects on C. elegans which may be developed as a natural supplement agent.展开更多
基金Supported by Guizhou Provincial Science and Technology Project(2024-023ZK2024-047,2024-015)+3 种基金the Innovation and Entrepreneurship Training Program for Undergraduates from China(202310660082,S2024106601432X)University Engineering Research Center for the Prevention and Treatment of Chronic Diseases by Authentic Medicinal Materials in Guizhou Province(2023-035)Administration of Traditional Chinese Medicine of Guizhou Province(QZYY-2024-134)Science Foundation of the Health Commission of Guizhou Province(gzwkj2025-538).
文摘[Objectives]To investigate the anti-hepatic fibrosis mechanism of lavandulyl flavonoid Kurarinol A(KA)from Sophora flavescens through the TGF/Smad signaling pathway.[Methods]A hepatic fibrosis model was established by TGF-β1-induced activation of human hepatic stellate cells LX-2.Western blot and RT-qPCR techniques were employed to study the anti-fibrotic mechanism of KA through the TGF/Smad signaling pathway.[Results]KA exerted anti-hepatic fibrosis effects by significantly reducing the gene expression levels of TGF-β1,Smad2,Smad3,and Smad4,as well as markedly decreasing the protein expression levels of TGF-β1,p-Smad2/3/Smad2/3,and Smad4.[Conclusions]KA demonstrates significant anti-hepatic fibrosis activity and alleviates liver fibrosis through the TGF/Smad signaling pathway.
基金supported by the National Natural Science Foundation of China(82274225)NATCM's Project of High-level Construction of Key TCM Disciplines-Beijing University of Chinese Medicine-Life Science from the Perspective of Chinese Medicine(zyyzdxk-2023263).
文摘Objective:To assess the efficiency of a Sophora flavescens Ait(S.flavescens,Ku Shen)-soluble microneedle(SFA-MN)for improving skin lesion symptoms in mice with psoriasis.Methods:SFA-MNs were prepared using a two-mold molding process with 20%w/v poly-vinylpyrrolidone and 15%w/v polyvinyl alcohol.The SFA-MNs were assessed for morphology,mechanical properties,in vitro dissolution,identification of components,and skin lesion improvement in imiquimod-induced psoriasis mice.Results:The SFA-MNs demonstrated good mechanical properties for efficiently penetrating the dermis,facilitating efficient drug delivery.Furthermore,they effectively inhibited mast cell levels in the dorsal lesion area of psoriasis mice and reduced the expression of the T-lymphocyte factor cluster of differ-entiation 3 and tumor necrosis factor-a.In addition,this system alleviated skin inflammation,splenic swelling,and thymic atrophy in the psoriasis-like mouse model.Seven major components were detected from SFA-MNs by comparison of the mass-to-nucleus ratios(m/z)of the secondary fragments N-methylcytisine,5a,9a-dihydroxymatrine,sophoramine,matrine,oxysophocarpine,oxymatrine,and kushenol O.Conclusion:The drug delivery strategy combining traditional herbal S.flavescens with soluble micro-needle technology provides more targeted and effective immune regulation for treating psoriasis-like mice models,enabling enhanced therapeutic effects compared with the control group.
文摘In the context of a surging demand for functional foods,this study utilized Sophora japonica L.(SL)and Rosa rugosa Thunb.(RT),which are rich in polyphenols(with flavonoids as the core subclass).High-purity extracts(SLE and RTE)were obtained through ethanol reflux extraction and macroporous resin purification,and then formulated with maltodextrin and erythritol to prepare a composite solid beverage.This beverage exhibited excellent antioxidant capabilities.At a concentration of 1 mg/mL,the scavenging rates of DPPH,ABTS,and hydroxyl radicals reached 82.4%,94.6%,and 49.2%,respectively.Network pharmacology indicated that quercetin andβ-sitosterol could modulate lipid metabolism pathways.Moreover,the beverage showed potential for lipid-lowering.Its cholate adsorption capacity was 589.4±2.9 mg/g at pH 7.0,and the IC50 value for pancreatic lipase inhibition was 32.55 mg/mL.However,a 60-day storage stability test revealed that the moisture content approached 5%,likely due to polyphenol-flavonoid reactivity,extending dissolution time to 30.88 s.These changes were attributed to polyphenols(with flavonoids as the core active subclass,and non-flavonoids such as phenolic acids as auxiliary),resulting in color alterations and reduced solubility.This study confirmed the dual functions of the SL-RT beverage in antioxidant and lipid-lowering aspects.Nevertheless,it also pointed out the need to optimize the formula and process to enhance stability,providing an important basis for the development of stable functional beverages.
基金supported High-level Innovative Talents of Guizhou Province(QianKeHe platform talents-GCC[2023]047)Guizhou Province"14th Five Year Plan"key discipline of traditional Chinese medicine and ethnic medicine(QZYYZDXK(JS)-2021-03)Guizhou Administration of Traditional Chinese Medicine,Traditional Chinese medicine,ethnic medicine science and technology research project(QZYY-2021-098).
文摘Background:Sophorae Tonkinensis Radix et Rhizoma(ST,also known as ShanDouGen)pertains to the Sophora genus,which is generally distributed in Southwest China.As a celebrated folk medicine,it has heat-clearing and detoxifying,reducing swelling and soothing pharynx.In recent years,there has been a notable rise in adverse events,including hepatotoxicity,associated with the use of ST,however,the mechanism behind ST-induced hepatotoxicity is unclear.Methods:The effects of ST on liver injury were investigated in vivo.Then,serum and liver samples were then analyzed using high-throughput metabolomics techniques.Furthermore,the application of network pharmacology and multivariate statistical analysis has been instrumental in identifying and predicting biomarkers and targets linked to liver toxicity.Results:In this study,we found that ST extract has a certain degree of damage to the rat liver.Using UPLC-MS/MS techniques,463 compounds were identified from ST,of which 73 compounds were absorbed in the blood of the STH group.Metabolomics results showed that amino acid metabolic biomarkers were associated with liver toxicity induced by ST.Notably,we identified 22 core ingredient as toxic effects Q-markers of ST through correlation analysis between biomarkers and absorbed components.On this basis,it is concluded by network pharmacology that ST may cause liver toxicity through PI3K-Akt signaling pathway,NF-kappa B signaling pathway,MAPK signaling pathway,and other pathways.Conclusion:Our findings also demonstrated that the“chemical composition-blood migration component-liver metabolism”stactics has an enormous potential to discern biomarkers and ingredients,and to elaborate the complexity toxicity mechanism of ST.
文摘A lectin protein(SFL) with molecular weight about 32 kD which markedly agglutinated rabbit and human red blood cells was purified from the roots of Sophora flavescens Ait. This protein, and apparently inhibited the growth of Fusarium vasinfectum Atk., Gibberella saubinetii (Mont.) Sacc., and Piricularia oryzae Cav. A set of degenerate PCR primer was synthesized according to the N-terminal sequence of the purified protein. The full-length cDNA coding the lectin was cloned by RT-PCR and 5'-RACE and sequenced (GenBank AF285121). The deduced amino acid sequence indicates that a preprotein with 284 amino acid residues is firstly translated and then processed to a mature protein with 254 amino acids. A N-Glycosylation site is the Asn 182 residue.
基金Supported by National Natural Science Foundation of China(31100987)Project of Shandong University of Technology(4040-306018)Young Teacher Development Plan of Shandong University of Technology~~
文摘[Objective] We aimed to investigate the preventive and therapeutical effect of compound of traditional Chinese drugs （Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root） on acute alcohol intoxication in mice. [Method] Acute alcohol intoxication was induced by administering alcohol to mice. Three different doses （low, middle and high） of compound of traditional Chinese drugs were administered to mice before and after administering alcohol respectively to investigate the preventive and therapeutical effect of drugs on acute alcohol intox-ication through doing statistical analysis about drunk mice and their sleeping time. The concentration of malondialdehyde （MDA）, reduced glutathione （GSH） and triglyc-erides （TG） in liver was also determined to investigate the protective effect of drugs on liver. [Result] The efficacy of compound of traditional Chinese drugs on acute al-cohol intoxication was dose-dependent. High-dose administration decreased the number of drunk mice significantly compared with control group; middle- and high-dose administration reduced the sleeping time of drunk mice and the concentration of MDA and TG in liver tissue; three doses al increased the concentration of GSH. [Conclusion] The compound of Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root had preventive and therapeutical effect on hangover, and it also had certain preventive and therapeutical effect on liver damage caused by alcohol.
基金Project supported by the Scientific Project of the Dezhou Government (2006067)the Science Fundation of Shandong Province (Q2008B08)the Key Technologies R&D Programme of Shandong Province (2010GSF10615)
文摘Flos Sophorae and Fructus Sophorae are two kinds of traditional Chinese medicines. In this work, the two kinds of traditional Chinese medicines collected from eleven areas of Dezhou, were analyzed by inductively couple plasma-mass spectrometry （ICP-MS） to compare the content and distribution of 14 kinds of rare earth elements （REEs）. The method was verified by analyzing GBW07605 certified reference material. The results showed that ICP-MS is an accurate, sensitive and reliable technique for determining REEs in traditional Chinese medicine. There were big differences in contents for REEs in Flos Sophorae and Fmctus Sophorae from different areas. The contents of total REEs in Flos Sophorae samples from different areas ranged from 1.0785 to 2.2659 μg/g, while those in Fmctus Sophorae from 0.6826 to 1.0527 ktg/g. The contents of total REEs in Flos Sophorae samples from different areas were obviously higher than those in Fmctus Sophorae of the same area and there was big difference between various Flos Sophorae samples. Interestingly, the higher the content of total REEs in Flos Sophorae samples, the lower the content of total REEs in Fmctus Sophorae samples of the same area. The plots of normalized element concentration versus atomic number showed some characteristic distribution trends. The distribution trend of light REEs （La-Gd） was relatively fiat except a positive Eu anomaly, however, that was steep and discrepant for heavy REEs （Tb-Lu）. The results could provide a valuable reference for understanding the relationship between the curative mechanism, pharmacology characteristics and their geological condition for the two traditional Chinese medicines investigated.
基金supported by the Natural Science Foundation of China(Nos.30672597 and 81330089)
文摘Sophorae Flavescentis Radix(Sophora flavescens Ait., SFR) and Sophorae Tonkinensis Radix et Rhizoma(S. tonkinensis Gapnep., STR) are two commonly used traditional Chinese medicines from Sophora(Leguminosae) plants, which are believed to possess similar bioactive components with entirely different clinical applications. In order to find out the characteristic chemical constituents potentially leading to the unique medicinal properties claimed for each of the two closely related TCMs, an HPLC fingerprint method was developed for analyses of the alkaloid and flavonoid constituents of SFR and STR, respectively, which were further evaluated and compared through similarity calculation and hierarchical clustering analysis(HCA). The results from the present study showed that the alkaloid fingerprints of the two herbs were similar, with many components co-existing in both drugs and various batches of samples from different species being mixed together in the HCA dendrogram. However, their flavonoid constituents were totally different with specific fingerprints being yielded for each herb, and further HCA analysis showed that the tested samples could almost be clearly divided into two groups based on their origins of species. The results from the present study indicated that the flavonoid constituents could serve as the differentially diagnostic constituents of SFR and STR and might potentially attributed to their distinct therapeutic effects.
基金Supported by National Natural Science Foundation of China(30960010)KeyProject of Principal Fund in Tarim University(TDZKZD06001)~~
文摘[ Objective ] The paper was to study and analyze the genetic diversity of 48 endophytic bacteria isolated from Sophora alopecuroide with strong antagonis- tic effect against Fusarium oxysporum and Verticillium dahliae, and carry out sequence measurement and phylogenetic analysis on 10 representative strains. [ Meth- od] Using total DNA ERIC-PCR and 16S rRNA sequencing analysis method, a total of 48 endophytic bacteria were carried out genetic diversity and phylogenetic analysis. [Result] ERIC-PCR fingerprint map showed that 48 endophytic bacteria were divided into 6 ERIC groups and 2 strains with independent groups at Wat- son distance of 0.31. Representative strains were selected from each group to determine 16S rRNA gene for phylogenetic analysis, and the results showed that these strains belonged to Bacillus atrophaeus, Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Bacillus pumilus and Serratia marcescens, respectively. [ Conclu- sion ] The genetic diversity of 48 strains of bacteria was obvious. Determination of 48 strains of bacteria on diversity and phylogenetic status could lay foundation for study on mechanism of F. oxysporum and V. dahliae, which could also provide new strain sources for biological control of diseases in cotton production.
基金Supported by National Natural Science Foundation of China(41076097,41006097)Science and Technology Research Key Project of ChineseMinistry of Education(211065)+3 种基金Natural Science Foundation of Jiangsu Province,China(BK2010322)Open Research of Jiangsu Key Laboratory of Environmental Material and Environmental Engineering(K090027,K090025,K090026,K090028)Graduate Science and Technology Innovation Project of Department of Education,Jiangsu Province,China(M080960)"New Century"Talent Project of Yangzhou University,China~~
文摘[Objective] The research aimed to discuss the inhibitory effect of Sophora japonica extracts against the growth of Microcystis aeruginosa.[Method] The inhibitory effect of extracted liquid of Sophora japonica leaf against the growth of M.aeruginosa was measured.Moreover,the active component was studied and analyzed initially.[Result] The absolute alcohol extract of Sophora japonica leaf was separated by n-hexane,ethyl acetate,n-butanol and water phases in turn.The polar fractions were found being the majority （〉60%）.The non-polar fraction in n-nexane （about 25%） was found significantly inhibiting the growth of M.aeruginosa.The inhibition rates of fraction in n-hexane at the concentrations of 25 and 50 mg/L against M.aeruginosa in 7 d were higher than 75% and 90% respectively.In addition,chlorophyll a of M.aeruginosa was also destroyed in the presence of the hexane fraction.[Conclusion] The research provided the theoretical basis for preventing and controlling the water bloom of M.aeruginosa.
文摘The method of cultivating seedlings of Sophora japonica f.flavi-rameus on rootstocks of Sophora japonica Linn.was introduced in this study,including rootstock cultivation,grafting and post-grafting management,specifically,seed collection,sowing,and breeding large seedlings in the cultivation of rootstocks;grafting time,scion collection and treatment,rootstock treatment,as well as techniques of stump grafting and bud grafting;focuses of post-grafting management,such as checking the survival conditions and untying the film,bud picking,pinching,topiary work,moisture and fertilization management,disease and pest control.Then the application of Sophora japonica f.flavi-rameus in gardens was analyzed,and it was proposed that biological characteristics and aesthetic principles should be followed,proper planting patterns should be applied,such as isolated planting,group planting,mass planting,linear planting and so on,moreover,it could also be combined with other species to create outstanding landscape effects.
基金financially supported by the National Natural Science Foundation of China(Nos.32160103 and U1812403)the Science and Technology Department of Guizhou Province(Nos.QKH ZC-[2021]-YB181,QKH CXTD-[2022]-007 and QKH ZYD-[2022]-4015)Guizhou Provincial Engineering Research Center for Natural Drugs。
文摘Three novel matrine-type alkaloids(1-3)and two unprecedented aloperine-type alkaloids(4 and 5)were isolated from the root of Sophora tonkinensis and the seeds of Sophora alopecuroides respectively.Notably,compound 1 possessed an unprecedented 6/5/6 tricyclic skeleton,while compounds 2 and 3 characterized by rare 6/6/5/6 tetracyclic system and 6/6/6/6/6 pentacyclic system respectively.Moreover,compound 4 possessed an unprecedented 6/7/6/6 tetracyclic core,and compound 5 characterized by rare 6/6/6/6tetracyclic skeleton.Their structures were elucidated by comprehensive spectroscopic data analysis and electronic circular dichroism(ECD)calculations.Biological tests indicated that compound 5 displayed significant anti-tobacco mosaic virus(TMV)activity compared with the positive control ningnanmycin.
基金supported by the National Science Foundation of China(No.31360604)
文摘Sophora moorcroftiana(S. moorcroftiana) is an endemic leguminous dwarf shrub in Tibet, China. Decoctions of the seeds have been used in Chinese folk medicine for dephlogistication, detoxication, and infectious diseases. The present study aimed to investigate the constituent and biological effects of polysaccharides from S. moorcroftiana seeds in Caenorhabditis elegans(C. elegans). Polysaccharides from S. moorcroftiana seeds(SMpol) were extracted with 60% ethanol and constituent was analyzed by GC-MS. SMpol was composed of glucose, galactose and inositol in the molar ratio of 35.7 : 1.3 : 17.0. Synchronized worms were treated with SMpol and then lifespan, motility, reproduction, stress resistance and antimicrobial activity were examined. Compared with the control group, the lifespan was increased to the average of 27.3 days and the number of laying eggs showed a 1.3-fold increase in nematodes treated with SMpol(4 mg×mL^(–1)). In SMpol(4 mg×mL^(–1)) treated worms, there was a 1.1-fold increase in 24-h survival of acute heat stress and a 1.6-fold increase in 2-h survival of oxidative stress The colonization of the bacteria in the SMpol treated nematode was significantly lower than that of the untreated group by 68.3%. In vivo studies showed SMpol significantly extended the life span, improved reproduction, increased stress resistance and antimicrobial capacity of C. elegans. In conclusion, those results indicated that the polysaccharides from S. moorcroftiana seeds were involved in a variety of biological activities leading to its modulatory effects on C. elegans which may be developed as a natural supplement agent.
