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SOME/IP在车载安卓系统中的研究与应用
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作者 谭伟浩 孙庆岩 +2 位作者 刘勋邦 齐思伟 刘芳芳 《汽车电器》 2025年第8期99-103,共5页
随着汽车新技术的不断发展,电子电气系统日益复杂化,以太网、面向服务架构SOA、汽车开放系统架构AUTOSAR等先进技术成为发展的重要方向。为满足可扩展性和以太网兼容,AUTOSAR联盟提出了基于网络层IP的可扩展的面向服务的中间件SOME/IP... 随着汽车新技术的不断发展,电子电气系统日益复杂化,以太网、面向服务架构SOA、汽车开放系统架构AUTOSAR等先进技术成为发展的重要方向。为满足可扩展性和以太网兼容,AUTOSAR联盟提出了基于网络层IP的可扩展的面向服务的中间件SOME/IP协议。本文详细阐述SOME/IP协议在车载Android系统中的集成,通过引入vsomeip等第三方库,利用Java本地接口JNI实现SOME/IP在Android系统中的应用,并结合应用案例实现了Ubuntu与Android系统间的SOME/IP通信。随着集成技术的成熟,SOME/IP将更深入地融入Android生态系统,进而促进设备与应用间通信,并展现出积极且具有潜力的前景。 展开更多
关键词 以太网 面向服务的架构 some/IP ANDROID
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基于车载以太网SOME/IP协议的研究及其在商用车中的应用 被引量:1
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作者 任勇 刘康 +2 位作者 王静 王涛 李小燕 《汽车电器》 2024年第5期47-50,共4页
由于汽车智能化、网联化的进一步发展,车载以太网不仅在乘用车领域得到了越来越广泛的应用,而且在商用车领域也逐步得到了应用。目前国内乘用车已经逐步采用SOME/IP的通信方案,商用车采用SOME/IP通信技术的报道较少。本文主要介绍SOME/I... 由于汽车智能化、网联化的进一步发展,车载以太网不仅在乘用车领域得到了越来越广泛的应用,而且在商用车领域也逐步得到了应用。目前国内乘用车已经逐步采用SOME/IP的通信方案,商用车采用SOME/IP通信技术的报道较少。本文主要介绍SOME/IP数据格式和通信机制,并着重介绍在OTA升级中的应用、SOME/IP的测试内容及相关测试实例。 展开更多
关键词 车载以太网 some/IP 协议测试 OTA
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基于SOME/IP协议的汽车通信数据异常检测方法研究 被引量:1
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作者 李林桥 方锴 《时代汽车》 2024年第17期7-9,共3页
SOME/IP协议作为一种重要的汽车通信中间件协议,为车辆之间和车辆与基础设施之间的数据交换提供了高效的解决方案。本文基于SOME/IP协议研究一种新的汽车通信数据异常检测方法,针对复制攻击、断连攻击和发布订阅攻击等进行深入研究,引入... SOME/IP协议作为一种重要的汽车通信中间件协议,为车辆之间和车辆与基础设施之间的数据交换提供了高效的解决方案。本文基于SOME/IP协议研究一种新的汽车通信数据异常检测方法,针对复制攻击、断连攻击和发布订阅攻击等进行深入研究,引入SOME/IP协议进行数据交互和导向,利用广播一致性检测、请求响应配对检测、网络流量分析识别攻击的异常通信数据,并引入加密与签名技术,从而有效识别和防御潜在的安全威胁,提高车载网络通信的安全性和可靠性。 展开更多
关键词 some/IP协议 汽车通信 通信数据 异常检测
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车载以太网SOME/IP测试的研究与分析 被引量:8
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作者 李志涛 姬志博 耿伟峰 《汽车电器》 2022年第6期95-98,共4页
随着汽车技术的飞速发展,车辆智能化、网联化、电动化快速推进,软件定义汽车成为关键,SOA设计方法引用至汽车软件开发中,车辆诊断、软件刷新、影音娱乐、智能驾驶等功能日益增多,对汽车电子电器的开发提出了新的挑战。为了实现技术整合... 随着汽车技术的飞速发展,车辆智能化、网联化、电动化快速推进,软件定义汽车成为关键,SOA设计方法引用至汽车软件开发中,车辆诊断、软件刷新、影音娱乐、智能驾驶等功能日益增多,对汽车电子电器的开发提出了新的挑战。为了实现技术整合,满足用户驾驶功能、性能需求,采用基于以太网技术的SOA架构几乎是所有整车厂关注的焦点,而面向服务的通信协议SOME/IP是实现SOA的重要一环。本文基于车载以太网测试工作实践,阐述SOME/IP协议应用需求、通信机制、SOME/IP协议测试及相关实例等,以便为车载以太网测试方面的同仁提供一些参考。 展开更多
关键词 车载以太网 SOA some/IP 协议 服务
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洛克教育思想的未解之谜——以Some Thoughts Concerning Education为线索 被引量:2
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作者 韦永琼 《高等理科教育》 2017年第3期7-14,共8页
近年来国内兴起了新一轮的洛克研究热潮,主要集中于洛克政治哲学方面的研究。洛克流亡荷兰期间,出版了其生前唯一一本以"教育"一词命名的著作——Some Thoughts Concerning Education,到目前为止,此书仍未有一个统一认可的汉... 近年来国内兴起了新一轮的洛克研究热潮,主要集中于洛克政治哲学方面的研究。洛克流亡荷兰期间,出版了其生前唯一一本以"教育"一词命名的著作——Some Thoughts Concerning Education,到目前为止,此书仍未有一个统一认可的汉语译名。这实际透露出洛克的教育论说此前在教育学界并未得到真正符合其本义的透彻理解。经梳理与分析后认为:Some Thoughts Concerning Education其实尤为洛克本人所重视!它与《人类理解论》《政府论》等一同构成了洛克学说的整体核心部分。从洛克政治哲学的整体设计高度上看待他的家庭幼儿教育理论与实践,是以往研究未能充分关注到的。 展开更多
关键词 洛克研究 政治哲学 some THOUGHTS Concerning EDUCATION 幼儿家庭教育
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Some Suggestions for College Students to Improve Listening
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作者 苗琴 《海外英语》 2014年第7X期85-87,共3页
Language is the tool of communication. English is a compulsory course for every student. English listening, as an essential part of English learning, is an effective way to obtain new information. But for many student... Language is the tool of communication. English is a compulsory course for every student. English listening, as an essential part of English learning, is an effective way to obtain new information. But for many students, the most difficult part for them is listening. The paper aims to give some suggestions to the college English learners and to help them to improve their listening comprehension abilities. Such as: to strengthen language knowledge, to build up self-confidence, to use listening strategies, to learn western culture, and to listen extensively. 展开更多
关键词 ENGLISH LISTENING some suggestions IMPROVING liste
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基于AUTOSAR标准的ETH基础通信及SOME/IP通信实现 被引量:5
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作者 张弛 吴志红 +2 位作者 朱元 杜展志 王恩东 《信息通信》 2020年第2期7-12,共6页
概述了AUTOSAR标准的产生背景及基本架构;介绍了Vector MICROSAR ETH和SOME/IP的原理及基本应用;设计了使用Vector MICROSAR ETH进行TCP和UDP协议的通信方案以及使用SOME/IP进行以太网通信的方案;并在英飞凌TC397单片机上运行从而验证... 概述了AUTOSAR标准的产生背景及基本架构;介绍了Vector MICROSAR ETH和SOME/IP的原理及基本应用;设计了使用Vector MICROSAR ETH进行TCP和UDP协议的通信方案以及使用SOME/IP进行以太网通信的方案;并在英飞凌TC397单片机上运行从而验证了设计方案的可行性. 展开更多
关键词 AUTOSAR MICROSAR ETH some/IP 操作系统
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SOME/IP车载以太网服务协议的关键技术与性能分析 被引量:6
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作者 张毅峰 欧阳颂华 +2 位作者 魏鹏 丁炜超 郑佳明 《现代电子技术》 2023年第5期15-19,共5页
首先对SOME/IP协议服务发现(SD)模块和传输协议(TP)模块中客户端与服务端的通信行为与关键技术特性进行了详细分析;然后在Arm Linux环境下基于C++和CommonAPI构建了面向服务的车载应用开发通信架构,并在该架构下结合不同负载规格对SOME... 首先对SOME/IP协议服务发现(SD)模块和传输协议(TP)模块中客户端与服务端的通信行为与关键技术特性进行了详细分析;然后在Arm Linux环境下基于C++和CommonAPI构建了面向服务的车载应用开发通信架构,并在该架构下结合不同负载规格对SOME/IP服务协议的一致性、可靠性和可扩展性进行了详细测试。实验结果表明,SOME/IP协议面向服务的通信方式具备高可用性,能够有效提高整车的数据通信效率,降低车载应用软件开发的复杂性。 展开更多
关键词 车载以太网 some/IP协议 通信需求 ECU通信 性能分析 性能测试
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浅析《夏洛的网》中“SOME PIG”“TERRIFIC”和“RADIANT”的翻译
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作者 陈雪梅 《郑州航空工业管理学院学报(社会科学版)》 2016年第5期117-120,共4页
E.B.怀特的Charlotte’s Web(《夏洛的网》)以其优美朴实的语言与感人至深的故事情节深受人们喜爱,文章评析了康馨和任溶溶对"SOME PIG""TERRIFIC"和"RADIANT"这三个词的中文翻译,认为译文除须准确达意... E.B.怀特的Charlotte’s Web(《夏洛的网》)以其优美朴实的语言与感人至深的故事情节深受人们喜爱,文章评析了康馨和任溶溶对"SOME PIG""TERRIFIC"和"RADIANT"这三个词的中文翻译,认为译文除须准确达意且考虑儿童认知外,还须契合标识语简洁易认的特征。作者认为原作品是儿童文学,那么译作就应该像童话;原作品语言生动朴实,译文也就应该简单活泼;这三个词拼写简单,译文也应该短小凝练。 展开更多
关键词 《夏洛的网》 somePIG” “TERRIFIC” “RADIANT” 任溶溶 康馨
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车载以太网SOME/IP在信息娱乐系统的应用 被引量:4
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作者 赵颖慧 白杨 赵聪聪 《汽车文摘》 2020年第4期31-34,共4页
随着汽车智能化和娱乐化的不断发展,传统CAN通讯已经无法满足车载娱乐系统对传输带宽的要求,迫切需求高带宽的新一代车载网络技术及架构。因此,车载以太网应运而生,其高传输速率能够满足现代汽车娱乐系统的音频、视频、云端等数据传输... 随着汽车智能化和娱乐化的不断发展,传统CAN通讯已经无法满足车载娱乐系统对传输带宽的要求,迫切需求高带宽的新一代车载网络技术及架构。因此,车载以太网应运而生,其高传输速率能够满足现代汽车娱乐系统的音频、视频、云端等数据传输的要求。SOME/IP协议是车载以太网的重要应用层协议之一,掌握SOME/IP的应用方法对车载信息娱乐模块至关重要。 展开更多
关键词 车载以太网 some/IP 协议 车载信息娱乐
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车载以太网SOME/IP协议及一致性测试系统研究 被引量:5
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作者 曹丽平 徐维 刘敏 《汽车电器》 2023年第6期55-58,62,共5页
随着汽车网联化、智能化、电动化的快速发展,车载以太网已成为新型网络架构中的骨干网络。对汽车而言,汽车的自主度越高,系统故障导致的后果也将变得越来越严重,并且国内车载以太网技术处于发展的初期,为保证这些系统安全可靠地运行,车... 随着汽车网联化、智能化、电动化的快速发展,车载以太网已成为新型网络架构中的骨干网络。对汽车而言,汽车的自主度越高,系统故障导致的后果也将变得越来越严重,并且国内车载以太网技术处于发展的初期,为保证这些系统安全可靠地运行,车载以太网协议一致性测试显得尤为重要。SOME/IP属于车载以太网架构中应用层和传输层之间的中间件,对于SOME/IP而言,它是面向服务的通信。SOME/IP协议一致性测试包括SOME/IP Server测试及SOME/IPETS测试,测试结果表明,该测试系统能够有效检测出DUT在服务请求及服务应答过程中的错误,为供应商及主机厂完善产品协议规范提供帮助。 展开更多
关键词 车载以太网 协议一致性 some/IP 面向服务
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浅谈英语中some和法语中quelque的用法
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作者 徐海燕 《黑龙江教育学院学报》 2014年第2期158-159,共2页
在语义上,英语中some对应法语的quelque。它们都可以做副词、泛指形容词、泛指代词、构词成分,用法极多。基于此,从它们各自的用法出发,通过静态的分析展示其使用特点,比较二者在使用上的异同。
关键词 some quelque 英法对比
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Some Oxidative Stress Biomarkers among Patients with Prostate Cancer in Sokoto, North Western Nigeria
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作者 Osaro Erhabor Asmau Muhammad Hussaini +2 位作者 Abdullahi Abdulwahab-Ahmed Michael Retsky Tosan Erhabor 《Open Journal of Blood Diseases》 CAS 2022年第3期60-78,共19页
Globally, prostate cancer (PCa) is the most common malignancy and the second leading cause of cancer-related death in men. It is a significant contributor to the burden of diseases and affects over a million men. This... Globally, prostate cancer (PCa) is the most common malignancy and the second leading cause of cancer-related death in men. It is a significant contributor to the burden of diseases and affects over a million men. This study investigated the levels of malondialdehyde and plasma total antioxidant capacity among patients with prostate cancer in Sokoto. This case-control study was conducted among 28 confirmed prostate cancer patients attending the Urology clinics in Usmanu Danfodiyo University Teaching Hospital and Sokoto Specialist Hospital in North Western Nigeria. Twenty-eight age-matched healthy males were monitored as controls. Determination of Total Antioxidant Capacity (TAC) was determined using Ferric Reducing Antioxidant Power (FRAP) reagent while the Malondialdehyde in serum was determined as a conjugate with Thiobarbituric acid (TBA) acid. Data were collected using a semi-structured interviewer-administered questionnaire. Data were processed using SPSS version 20 and results were reported as Mean ± Standard deviation. The malondialdehyde level was significantly increased (p < 0.0001) among subjects with prostate cancer (0.215 ± 0.06) compared to controls (0.073 ± 0.04). The plasma total antioxidant capacity decreased significantly among the subjects (247.9 ± 63.3) compared to controls (743.3 ± 104.40) (p < 0.0001). The findings from this indicated a high Malondialdehyde (lipid peroxidation indicator) and low levels of Total Antioxidant Capacity among prostate cancer patients as evidence of redox imbalance. Subjects in monogamous relationships compared to polygamous, rural dwellers, farmers, individuals of Hausa ethnicity and subjects who reported no family history of the disease were more predisposed to prostate cancer. Further epidemiological studies are needed to determine the predisposing factors and the potential role of these markers in the diagnosis, prognosis and management of prostate cancer patients in Sokoto in particular and Nigeria in general. We recommend that Malondialdehyde and Total Antioxidant Capacity be routinely monitored among patients with prostate cancer patients in the area. 展开更多
关键词 some Oxidative Stress Biomarkers Prostate Cancer Sokoto North Western Nigeria
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Evaluation of the Influence of Fixed Orthodontic Treatment Duration on the Severity of Inflammatory Gingival Enlargement (Fixed Orthodontic Induced Gingival Enlargements) and Some Properties of Saliva
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作者 Mohammed M. A. Abdullah Al-Abdaly Abdullah Mohammed A. Asiri +5 位作者 Ghadeer Mohammed Mohammed Al-Abdaly Mohammed Abdullah Ghabri Moayad Abdullah H. Alqaysi Abdulmajeed Mohammed S. Aljathnan Yahya Saleem M. Al Naser Naser Ali N. Alshahrani 《International Journal of Clinical Medicine》 2022年第3期132-146,共15页
Background: Inflammatory gingival enlargement is a more common clinical feature with orthodontic therapy than other features. Therefore, this study was designed to the evaluation of the influence of fixed orthodontic ... Background: Inflammatory gingival enlargement is a more common clinical feature with orthodontic therapy than other features. Therefore, this study was designed to the evaluation of the influence of fixed orthodontic treatment duration on the severity of inflammatory gingival enlargement (fixed orthodontic induced gingival enlargements) and some properties of saliva. Material and Methods: The sample size comprised 145 patients undergoing fixed orthodontic treatment for at least 6 months aged 13 - 32 years. They were divided according to orthodontic treatment duration into three groups. Group I (n = 47) included the patients who were treated for less than 6 months, group II (n = 51) included the patients who were treated for a period of 6 - 12 months, and group III (n = 47) included the patients who were managed for more than 12 months. Data were obtained from the outpatient clinics, college of dentistry, King Khalid University, Abha, Saudi Arabia, and some dental centers in Sana’a city, the Republic of Yemen. This study was conducted from October 2021 G to January 2022 G. Clinical examination was done for plaque index (PLI), gingival index (GI), and gingival enlargement indexes (GEI). Saliva was collected in sterile test tubes then salivary flow and pH were measured. Statistical analysis was done with SPSS (version 23) and ANOVA test to evaluate the impact of orthodontic treatment duration on the severity of inflammatory gingival enlargement and some properties of saliva. Results: The statistical analysis demonstrated the highest mean plaque index (PLI) was among groups III and I participants whereas, the highest mean gingival index and mean gingival enlargement were among groups II and III participants. The present study revealed an increase in salivary flow with decreased salivary pH values with an increase in orthodontic therapy duration. There were statistically significant differences in clinical findings and salivary flow and pH values were observed in the comparison between groups I, II and III except PLI (p Conclusion: There was a higher inflammatory gingival enlargement associated with a higher plaque index in patients under orthodontic treatment for more than 12 months more than the patients for less than 6 months and the patients for a period of 6 - 12 months. There were correlations between an increase of salivary flow and pH values and an increase of other variables in this study, such as plaque index, gingival index, and gingival enlargement index with an increased orthodontic therapy duration. 展开更多
关键词 Inflammatory Gingival Enlargement Orthodontic Treatment Duration some Salivary Properties
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基于语料库的《经济学原理》两个汉译本中“some”的翻译研究
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作者 杨露 瞿思敏 《科教文汇》 2018年第24期177-178,共2页
本文基于自建英汉平行语料库,结合定性分析和定量分析,对《经济学原理》梁小民译本和曹乾译本中some的翻译进行了分析。经考察,两译本中some对应的翻译在选词上有一定差别。从某种程度上来说,梁译本侧重忠实于原文内容,曹译本在选词上... 本文基于自建英汉平行语料库,结合定性分析和定量分析,对《经济学原理》梁小民译本和曹乾译本中some的翻译进行了分析。经考察,两译本中some对应的翻译在选词上有一定差别。从某种程度上来说,梁译本侧重忠实于原文内容,曹译本在选词上更富于灵活变化且更加注重表达的流畅性。 展开更多
关键词 some 语料库 《经济学原理》
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The Effect of Some Soil Physical and Chemical Properties on Soil Aggregate Stability in Different Locations in Sulaimani and Halabja Governorate
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作者 Salahaddin Abdulqadir Aziz Saman Mahmood Karim 《Open Journal of Soil Science》 2016年第4期81-88,共8页
Some soil properties were studied in relation soil aggregate along a climatologically region and different crop land use in the Northeast of Sulaimani City/Kurdistan Region of Iraq. Five locations were selected along ... Some soil properties were studied in relation soil aggregate along a climatologically region and different crop land use in the Northeast of Sulaimani City/Kurdistan Region of Iraq. Five locations were selected along these regions ranging from semiarid to sub-humid climatologically conditions. The soil physical, chemical properties, aggregate stability and size distribution were analyzed. A mean-weight-diameter (MWD) value was determined on 5 soils, which was the sum of the percentage of soil on each sieve (6, 3, 1.5, 0.75, 0.375 and 0.125 mm). The results of the measurements could evaluated with linear correlation coefficients for the relationships between aggregate stability (MWD) and soil physical and chemical properties of the different crop land use. The correlation coefficient for the relationship between aggregate stability and organic matter was highly significant (P < 0.0l%) which is in agreement with the findings of [1]. Generally large aggregates (large 6, 3, 3 - 1.5 mm) were present in highest proportions in the most semiarid of the studied areas. Aggregates 0.75 - 0.125 mm were positively correlated to fine, very fine sand and silt fractions and to organic matter. Stability of aggregates showed a positive correlation with clay content and organic matter content, while the carbonate content was strongly correlated with aggregate stability. The land use history affecting soil overlaps the pattern of climatological situations and cultivated crop lands and has to be taken into account. Aggregate size distribution and stability can be used as indicator of soil conservation and productivity. 展开更多
关键词 Aggregate Stability Organic Matter some Physical and Chemical Properties Soil Consistency Limits
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Some Group Runs Based Multivariate Control Charts for Monitoring the Process Mean Vector
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作者 Mukund Parasharam Gadre Vikas Chintaman Kakade 《Open Journal of Statistics》 2016年第6期1098-1109,共13页
In this article, we propose two control charts namely, the “Multivariate Group Runs’ (MV-GR-M)” and the “Multivariate Modified Group Runs’ (MV-MGR-M)” control charts, based on the multivariate normal processes, ... In this article, we propose two control charts namely, the “Multivariate Group Runs’ (MV-GR-M)” and the “Multivariate Modified Group Runs’ (MV-MGR-M)” control charts, based on the multivariate normal processes, for monitoring the process mean vector. Methods to obtain the design parameters and operations of these control charts are discussed. Performances of the proposed charts are compared with some existing control charts. It is verified that, the proposed charts give a significant reduction in the out-of-control “Average Time to Signal” (ATS) in the zero state, as well in the steady state compared to the Hotelling’s T2 and the synthetic T2 control charts. 展开更多
关键词 some Group Runs Based Multivariate Control Charts for Monitoring the Process Mean Vector
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基于SOME/IP的整车电气通信网络设计研究 被引量:4
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作者 李阳春 《汽车文摘》 2020年第8期32-38,共7页
汽车功能的发展依赖于汽车电子数据处理和传输能力的升级。数据传输能力升级的主要方式包括通过提升现有车载通讯速率。当前满足车载环境的以太网技术逐渐被应用,部分车厂已经有车载以太网应用的量产车型,以太网速度也逐渐从100 Mbps提... 汽车功能的发展依赖于汽车电子数据处理和传输能力的升级。数据传输能力升级的主要方式包括通过提升现有车载通讯速率。当前满足车载环境的以太网技术逐渐被应用,部分车厂已经有车载以太网应用的量产车型,以太网速度也逐渐从100 Mbps提升到1000 Mbps,车载以太网已经走向大规模应用。SOME/IP(Scalable service-Oriented MiddlewarE over IP)协议是基于车载以太网的通信接口,与当前汽车总线CAN面向信号的通信方式有很大不同,所以整车厂在应用车载以太网作为通信媒介设计电气通信网络架构时会出现很多问题,甚至是无法合理应用与开发。面向服务的通信方式是SOME/IP的关键特性和优势,如何有效与正确应用是基于车载以太网设计电气通信网络的关键,提供基于SOME/IP的整车电子电气通信网络设计方法。 展开更多
关键词 车载以太网 中间件 服务发现
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Some(Anti-)Self Duality Solutions on Six Manifolds
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作者 Ibrahim Sener 《Communications in Theoretical Physics》 SCIE CAS CSCD 2016年第10期379-384,共6页
The bundle of the 2-forms on 6-manifold decomposes into three subbundles such that Λ~2(R^6) = Λ~2_1⊕Λ~2_6⊕Λ~2_8 with dimensions 1,6 and 8,respectively.The self and anti self duality solutions of the 2-forms,call... The bundle of the 2-forms on 6-manifold decomposes into three subbundles such that Λ~2(R^6) = Λ~2_1⊕Λ~2_6⊕Λ~2_8 with dimensions 1,6 and 8,respectively.The self and anti self duality solutions of the 2-forms,called Φ-duality,are handled and these solutions show that the anti self dual gauge fields live on the subbundles Λ~2_1 and Λ~2_6 while the self ones equations on Λ28.Also the solution on Λ~2_1 presents a flat connection.In addition,the curvatures of the connections on Λ~2_6 and Λ~2_8 have Tr[F^3] = 0,and so the topological invariants determined by the Chern classes,i.e.topological charge,consist only on the second Chern class.In the result of this case,the anti self and self Φ-dual gauge invariant Lagrangians of defined on both subbundles are bounded by the same topological charge.Also,one gives a quantization case to be relating to the instanton number. 展开更多
关键词 six manifolds (anti-) self duality topological bound quantization
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线粒体自噬调控前列腺组织中NLRP3炎症小体的表达在实验性自身免疫性前列腺炎大鼠中的作用机制
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作者 陆良喜 陆海旺 +3 位作者 王文杰 史珺 黄志敏 宾彬 《实用医学杂志》 北大核心 2025年第12期1816-1824,共9页
目的探讨在实验性自身免疫性前列腺炎(experimental autoimmune prostatitis,EAP)大鼠前列腺组织中线粒体自噬调控NLRP3炎症小体的机制,以期为新药物研发提供理论支持。方法将40只SD雄性大鼠数字表随机分为8组,即正常组(N)、模型组(M)... 目的探讨在实验性自身免疫性前列腺炎(experimental autoimmune prostatitis,EAP)大鼠前列腺组织中线粒体自噬调控NLRP3炎症小体的机制,以期为新药物研发提供理论支持。方法将40只SD雄性大鼠数字表随机分为8组,即正常组(N)、模型组(M)、雷帕霉素组(RAP)、雷帕霉素+线粒体自噬抑制剂组(RAP+Mdivi-1)、自噬抑制剂组(3MA)、Caspase1抑制剂组(Caspase1)、线粒体自噬抑制剂组(Mdivi-1)、NLRP3抑制剂组(NLRP3),每组5只。药物干预后,采用HE染色、免疫荧光、比色法、WB法等观察相关指标。结果与N组比较,M组大鼠前列腺腺体结构损伤明显;与M组比较,RAP组、Caspase-1组、NLRP3组前列腺腺体结构有改善;而3-MA组、Mdivi-1组大鼠前列腺组织结构无改善,甚至破坏更明显。与N组比较,M组大鼠前列腺组织LC3-II和LAMP-1共表达增强,线粒体膜电位下降,ROS释放水平明显增加。与M组比较,RAP组、NLRP3组上述指标明显改善;但是,3-MA组、Mdivi-1组上述指标变得更差。与N组比较,M组大鼠前列腺线粒体DRP1、PINK1、Parkin蛋白表达升高,OPA1蛋白表达降低。与M组比较,RAP组、NLRP3组DRP1、PINK1、Parkin蛋白表达显著升高,RAP组OPA1蛋白表达显著降低;3-MA组、Mdivi-1组DRP1、PINK1、Parkin蛋白表达显著下降;Caspase-1组Parkin蛋白表达降低,但是DRP1、OPA1、PINK1蛋白表达差异无统计学意义。与N组比较,M组大鼠前列腺组织自噬蛋白LC3II/LC3I、Beclin1和炎症小体相关蛋白NLRP3、ASC、Cleaced-Caspase1、Cleaced-IL-1β、IL-18表达升高,P62蛋白表达下降;与M组比较,RAP组、NLRP3组LC3II/LC3I、Beclin1蛋白表达显著升高,P62、NLRP3、ASC、Cleaced-Caspase1、Cleaced-IL-1β、IL-18蛋白表达显著降低;3-MA组、Mdivi-1组LC3II/LC3I、Beclin1蛋白表达显著下降,P62、NLRP3、ASC、Cleaced-Caspase1、IL-18蛋白表达升高;与M组比较,Caspase-1组NLRP3、ASC、Cleaced-Caspase1、Cleaced-IL-1β、IL-18蛋白表达显著降低,LC3II/LC3I、Beclin1、P62蛋白表达差异无统计学意义。结论NLRP3炎症小体参与EAP大鼠前列腺炎症进程,线粒体自噬通过调控前列腺组织中NLRP3炎症小体的活化介导EAP大鼠前列腺炎的发生发展。 展开更多
关键词 慢性前列腺炎 线粒体自噬 Pink1/Parkin通路 NLRP3炎症小体 炎症
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