The development of insect appendages requires the expression of multiple genes in a strict spatial and temporal order. The odd-skipped family genes are vital transcriptional factors involved in embryonic development. ...The development of insect appendages requires the expression of multiple genes in a strict spatial and temporal order. The odd-skipped family genes are vital transcriptional factors involved in embryonic development. The development and morphogenesis of the insect wing requires multiple transcription factors to regulate the expression of wing patterning genes at the transcriptional level. However, the function of odd-related genes in insect wing morphogenesis and development during postembryonic stages is unclear. We focused on the roles of the sister of odd and bowl (sob) gene, a member of odd-skipped family genes, during the wing morphopoiesis in Bombyx mori using the clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 system and in Tribolium castaneum by RNA interference. The results showed that the wings were significantly smaller and degenerated, and wing veins were indistinct in the sob gene loss-of-function group in both B. mori and T. castaneum. Quantitative real-time polymerase chain reaction revealed that the Tcsob gene regulated the expression of wing development genes, such as the cht 7 and the vg gene. The findings suggest the importance of sob gene in insect wing morphology formation during postembryonic stages.展开更多
筛选高效脱硫菌株并揭示其去除硫化物的机理对生物脱硫技术的发展具有重要意义.从天然气净化厂污水站废水中分离出一株化能自养型硫氧化细菌,命名为DS-B.根据16S r DNA基因序列相似性分析,结合形态特征,对该菌株进行鉴定.进一步研究DS-...筛选高效脱硫菌株并揭示其去除硫化物的机理对生物脱硫技术的发展具有重要意义.从天然气净化厂污水站废水中分离出一株化能自养型硫氧化细菌,命名为DS-B.根据16S r DNA基因序列相似性分析,结合形态特征,对该菌株进行鉴定.进一步研究DS-B的硫代硫酸盐耐受性,并通过测定培养体系中各价态含硫物质的含量,探讨该菌的硫氧化性能.结果显示,菌株DS-B与Thioalkalivibrio thiocyanoxidans ARh2的序列相似性最高,初步鉴定其属于Thioalkalivibrio.DS-B的最佳生长和降解温度为35℃,35℃下培养7 d,硫代硫酸盐(S2O32-)的去除率能达到98.7%.该菌对S2O32-有较强的耐受性,且最适S2O32-浓度为2×104 mg/L.此外,对各价态硫氧化物的分析表明该菌氧化S2O32-的途径为:S2O32-→SO42-/S2O32-→S→SO42-.上述表明,菌株DS-B具有较强的耐盐能力,对硫代硫酸盐具有较高的去除能力,是一株具有应用价值的硫氧化细菌.展开更多
基金This project received funding from the National Natural Science Foundation of China(No.U20A2058,No.31830094,No.31472153)Funds of China Agriculture Research System(No.CARS-18-ZJO102).
文摘The development of insect appendages requires the expression of multiple genes in a strict spatial and temporal order. The odd-skipped family genes are vital transcriptional factors involved in embryonic development. The development and morphogenesis of the insect wing requires multiple transcription factors to regulate the expression of wing patterning genes at the transcriptional level. However, the function of odd-related genes in insect wing morphogenesis and development during postembryonic stages is unclear. We focused on the roles of the sister of odd and bowl (sob) gene, a member of odd-skipped family genes, during the wing morphopoiesis in Bombyx mori using the clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 system and in Tribolium castaneum by RNA interference. The results showed that the wings were significantly smaller and degenerated, and wing veins were indistinct in the sob gene loss-of-function group in both B. mori and T. castaneum. Quantitative real-time polymerase chain reaction revealed that the Tcsob gene regulated the expression of wing development genes, such as the cht 7 and the vg gene. The findings suggest the importance of sob gene in insect wing morphology formation during postembryonic stages.
基金Supported by the National Natural Science Foundation of China (31600299)the Youth Science and Technology Nova Program of Shaanxi Province,China (2022KJXX-01)the Scientific Research Program Funded by Yunnan Provincial Education Department,China (2022J0949)。
