BACKGROUND Type 2 diabetic nephropathy(T2DN)is a severe complication of diabetes mellitus,and identifying biomarkers for its prognosis remains a critical challenge.Previous studies have suggested potential roles of mi...BACKGROUND Type 2 diabetic nephropathy(T2DN)is a severe complication of diabetes mellitus,and identifying biomarkers for its prognosis remains a critical challenge.Previous studies have suggested potential roles of microRNAs(e.g.,miR-495-3p),adiponectin(ADPN),and cardiometabolic index(CMI)in metabolic and renal pathologies.However,their combined predictive value for T2DN prognosis is not well understood.AIM To explore serum miR-495-3p,ADPN,and CMI levels in T2DN and their value in predicting prognosis.METHODS A total of 98 T2DN patients(study group)and 49 type 2 diabetic patients with normal renal function(control group)were enrolled from February 2020 to February 2022.Serum levels of miR-495-3p,ADPN,and CMI were measured in both groups.Patients were followed up for 6 months to assess prognosis.Dif-ferences between groups were analyzed,and multivariate logistic regression and receiver operating characteristic(ROC)curve analyses were performed to eva-luate the predictive value of these biomarkers.RESULTS The study group exhibited significantly lower miR-495-3p levels and higher ADPN and CMI levels compared to the control group(P<0.05).Poor prognosis patients had even lower miR-495-3p and higher ADPN and CMI levels than those with good prognosis(P<0.05).Multivariate analysis identified alanine amino-transferase,aspartate aminotransferase,urea nitrogen,serum creatinine,miR-495-3p,ADPN,and CMI as independent predictors of prognosis(P<0.05).ROC analysis revealed area under the curve values of 0.762(miR-495-3p),0.902(ADPN),0.757(CMI),0.899(alanine aminotransferase),0.852(aspartate ami-notransferase),0.916(urea nitrogen),and 0.910(serum creatinine)for predicting poor prognosis(P<0.05).CONCLUSION Low miR-495-3p and high ADPN and CMI levels are linked to T2DN and poor prognosis,highlighting their potential for risk prediction and clinical management.展开更多
[目的]探究miR-325-3p靶向PRDX4对肾细胞癌细胞增殖、侵袭及凋亡的影响。[方法]设肾细胞癌细胞Caki-1组、miR-NC组、miR-325-3p-mimics组(过表达)、miR-325-3p-inhibitor组(低表达),测定各组细胞增殖、单克隆形成数目、凋亡率、侵袭水...[目的]探究miR-325-3p靶向PRDX4对肾细胞癌细胞增殖、侵袭及凋亡的影响。[方法]设肾细胞癌细胞Caki-1组、miR-NC组、miR-325-3p-mimics组(过表达)、miR-325-3p-inhibitor组(低表达),测定各组细胞增殖、单克隆形成数目、凋亡率、侵袭水平以及miR-325-3p、PRDX4水平。[结果]miR-325-3p-inhibitor组OD值(0.93±0.03)、存活率(86.58±6.36)%、单克隆形成数目(1062.29±102.78)、穿膜数(1917.34±425.35)、PRDX4 mRNA和蛋白表达水平(4.63±0.28、1.82±0.18)高于miR-325-3p-mimics组[(0.42±0.02)、(42.25±7.20)%、(239.89±35.27)个、(293.85±95.28)个、(2.04±0.24)、(0.38±0.07)](P<0.05),细胞凋亡率(1.12±0.29)%、miR-325-3p表达水平(1.42±0.38)低于miR-325-3p-mimics组(7.14±1.11)%、(5.68±0.37)(P<0.05)。[结论]miR-325-3p上调可以抑制肾细胞癌细胞的增殖(76.59%±7.30%vs 42.25%±7.20%)、迁移侵袭(702.28±111.52 vs 293.85±95.28),同时诱导细胞凋亡(3.46±1.04 vs 7.14±1.11),而这些过程主要是通过miR-325-3p与PRDX4的相互作用实现的。展开更多
文摘BACKGROUND Type 2 diabetic nephropathy(T2DN)is a severe complication of diabetes mellitus,and identifying biomarkers for its prognosis remains a critical challenge.Previous studies have suggested potential roles of microRNAs(e.g.,miR-495-3p),adiponectin(ADPN),and cardiometabolic index(CMI)in metabolic and renal pathologies.However,their combined predictive value for T2DN prognosis is not well understood.AIM To explore serum miR-495-3p,ADPN,and CMI levels in T2DN and their value in predicting prognosis.METHODS A total of 98 T2DN patients(study group)and 49 type 2 diabetic patients with normal renal function(control group)were enrolled from February 2020 to February 2022.Serum levels of miR-495-3p,ADPN,and CMI were measured in both groups.Patients were followed up for 6 months to assess prognosis.Dif-ferences between groups were analyzed,and multivariate logistic regression and receiver operating characteristic(ROC)curve analyses were performed to eva-luate the predictive value of these biomarkers.RESULTS The study group exhibited significantly lower miR-495-3p levels and higher ADPN and CMI levels compared to the control group(P<0.05).Poor prognosis patients had even lower miR-495-3p and higher ADPN and CMI levels than those with good prognosis(P<0.05).Multivariate analysis identified alanine amino-transferase,aspartate aminotransferase,urea nitrogen,serum creatinine,miR-495-3p,ADPN,and CMI as independent predictors of prognosis(P<0.05).ROC analysis revealed area under the curve values of 0.762(miR-495-3p),0.902(ADPN),0.757(CMI),0.899(alanine aminotransferase),0.852(aspartate ami-notransferase),0.916(urea nitrogen),and 0.910(serum creatinine)for predicting poor prognosis(P<0.05).CONCLUSION Low miR-495-3p and high ADPN and CMI levels are linked to T2DN and poor prognosis,highlighting their potential for risk prediction and clinical management.
文摘[目的]探究miR-325-3p靶向PRDX4对肾细胞癌细胞增殖、侵袭及凋亡的影响。[方法]设肾细胞癌细胞Caki-1组、miR-NC组、miR-325-3p-mimics组(过表达)、miR-325-3p-inhibitor组(低表达),测定各组细胞增殖、单克隆形成数目、凋亡率、侵袭水平以及miR-325-3p、PRDX4水平。[结果]miR-325-3p-inhibitor组OD值(0.93±0.03)、存活率(86.58±6.36)%、单克隆形成数目(1062.29±102.78)、穿膜数(1917.34±425.35)、PRDX4 mRNA和蛋白表达水平(4.63±0.28、1.82±0.18)高于miR-325-3p-mimics组[(0.42±0.02)、(42.25±7.20)%、(239.89±35.27)个、(293.85±95.28)个、(2.04±0.24)、(0.38±0.07)](P<0.05),细胞凋亡率(1.12±0.29)%、miR-325-3p表达水平(1.42±0.38)低于miR-325-3p-mimics组(7.14±1.11)%、(5.68±0.37)(P<0.05)。[结论]miR-325-3p上调可以抑制肾细胞癌细胞的增殖(76.59%±7.30%vs 42.25%±7.20%)、迁移侵袭(702.28±111.52 vs 293.85±95.28),同时诱导细胞凋亡(3.46±1.04 vs 7.14±1.11),而这些过程主要是通过miR-325-3p与PRDX4的相互作用实现的。
