Induced pluripotent stem （iPS） cells can be derived from human somatic cells by cellular reprograrnming. This technology provides a potential source of non-controversial therapeutic ceils for tissue repair, drug dis...Induced pluripotent stem （iPS） cells can be derived from human somatic cells by cellular reprograrnming. This technology provides a potential source of non-controversial therapeutic ceils for tissue repair, drug discovery, and opportunities for studying the molecular basis of human disease. Normally, mouse embryonic fibroblasts （MEFs） are used as feeder layers in the initial derivation of iPS lines. The pur- pose of this study was to determine whether SNL fibrohlasts can be used to support the growth of human iPS cells reprogrammed from somatic cells using lentiviral expressed reprogramming factors. In our study, iPS cells expressed common pluripotency markers, dis- played human embryonic stem cells （hESCs） morphology and unmethylated promoters of NANOG and OCT4. These data demonstrate that SNL feeder cells can support the derivation and maintenance of human iPS cells.展开更多
Summary The Laser intervention in early stages of age-related macular degeneration(LEAD)study(1)is a 36-month,multicenter,randomized,sham-controlled trial conducted from 2012-2015 of 292 participants with bilateral so...Summary The Laser intervention in early stages of age-related macular degeneration(LEAD)study(1)is a 36-month,multicenter,randomized,sham-controlled trial conducted from 2012-2015 of 292 participants with bilateral soft drusen,aka intermediate AMD(iAMD),who underwent q 6-month treatment with sub-threshold nanosecond laser(SNL)or sham treatment to the study eye.展开更多
基金supported by the National Institute of Health,USA (No.R21 RR025408)
文摘Induced pluripotent stem （iPS） cells can be derived from human somatic cells by cellular reprograrnming. This technology provides a potential source of non-controversial therapeutic ceils for tissue repair, drug discovery, and opportunities for studying the molecular basis of human disease. Normally, mouse embryonic fibroblasts （MEFs） are used as feeder layers in the initial derivation of iPS lines. The pur- pose of this study was to determine whether SNL fibrohlasts can be used to support the growth of human iPS cells reprogrammed from somatic cells using lentiviral expressed reprogramming factors. In our study, iPS cells expressed common pluripotency markers, dis- played human embryonic stem cells （hESCs） morphology and unmethylated promoters of NANOG and OCT4. These data demonstrate that SNL feeder cells can support the derivation and maintenance of human iPS cells.
基金This work was supported in part by NIH/NEI R01EY015520.
文摘Summary The Laser intervention in early stages of age-related macular degeneration(LEAD)study(1)is a 36-month,multicenter,randomized,sham-controlled trial conducted from 2012-2015 of 292 participants with bilateral soft drusen,aka intermediate AMD(iAMD),who underwent q 6-month treatment with sub-threshold nanosecond laser(SNL)or sham treatment to the study eye.
