Objective:To identify presence of inflammasome activated in mouse cochlea with sensorineural hearing loss (SNHL) caused by cytomegalovirus (CMV) infection. Method:MCMV was injected into the right cerebral hemisphere i...Objective:To identify presence of inflammasome activated in mouse cochlea with sensorineural hearing loss (SNHL) caused by cytomegalovirus (CMV) infection. Method:MCMV was injected into the right cerebral hemisphere in neonatal BALB/c mice at 2000 pfu virus titers. Auditory brainstem responses (ABRs) were tested to evaluate hearing at 21 days. Histopathological studies were conducted to confirm localizations of MCMV infected cells in the inner ear. Expression of inflammasome related factors was assessed by immunofluorescence, Quantitative real-time PCR and Western blotting. Results:In the mouse model of CMV induced SNHL, inflammasome related kinase Caspase-1 and downstream inflammatory factor IL-1b and IL-18 were found increased and activated after CMV infection in the cochlea. These factors could further up-regulate expression of IL-6 and TNF-a. These inflammatory factors are neurotoxicity and may contribute to hearing impairment. Furthermore, we also detected significantly increased AIM2 protein that accumulated in the SGN of cochleae with CMV infection. Significance:We have shown that inflammasome as a novel inherent immunity mechanism may contribute to hearing impairment. Conclusion:Our data indicate that imflammasome assemble in mouse inner ear in response to CMV infection. We have revealed a novel pa-thology event in CMV induced SNHL involving activation of inflammasome in mouse cochlea. Additionally, we have shown that inflammasome may be a novel target for prevention and treatment of CMV related SNHL. Copyright ? 2016, The Authors. Production & hosting by Elsevier (Singapore) Pte Ltd On behalf of PLA General Hospital Department of OtolaryngologyHead and Neck Surgery. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).展开更多
Background: Drill and surgical tools can cause noise induced hearing loss when used on or adjacent to the ossicular chain and stapes footplate or while working on the mastoid bone. Therefore the drill generated noise ...Background: Drill and surgical tools can cause noise induced hearing loss when used on or adjacent to the ossicular chain and stapes footplate or while working on the mastoid bone. Therefore the drill generated noise has been incriminated as a cause of sensorineural hearing loss in the operated ear. Objective: This study has been undertaken to estimate the proportion of sensorineural hearing loss following middle ear surgery and to assess the bone conduction thresholds of operated ear following middle ear surgery at different follow up periods. Methods: 100 cases of chronic suppurative otitis media posted for middle ear exploration surgery were included in the study. Results: Sensorineural hearing loss was present in 3 cases out of 100 cases (3%) after undergoing mastoid surgery. There was a mean increase in bone conduction thresholds of 0.75 dB at 500 Hz, 1.4 dB at 1 kHz, 1.7 dB at 2 kHz and 2.7 dB at 4 kHz at 6th month of follow up period after surgery with statistical significance at 2 kHz (p = 0.011) and 4 kHz (p Conclusion: Post mastoidectomy hearing loss is of high frequency in nature.展开更多
Aim: This study was carried out to assess association between Sensorineural hearing loss (SNHL) and Chronic Suppurative Otitis Media (CSOM), to identify possible risk factors and determine correlation of duration of C...Aim: This study was carried out to assess association between Sensorineural hearing loss (SNHL) and Chronic Suppurative Otitis Media (CSOM), to identify possible risk factors and determine correlation of duration of CSOM and incidence of SNHL. Material and Method: The study consisted of 100 patients of either sex between 15 and 40 years of age, suffering from unilateral CSOM for the past three months or more, the normal ear served as control. Results: This study showed duration of symptoms is 18% patients for 5 - 10 years and below 5 years in 73% patients. It was found that mean BC thresholds were significantly higher in the diseased ears as compared to the control ears. Though the higher frequencies are more affected than the lower frequencies, in subtotal and attic perforations the speech frequencies seemed to be maximally affected. Conclusion: CSOM should be diagnosed early and should be managed effectively so as to prevent the chances of developing SNHL.展开更多
8头颈癌患者放化疗和放疗后的感觉神经性耳聋：文献系统评价
Sensorineural hearing loss in patients with head and neck cancer after chemoradiotherapy and radiotherapy： A systematic review of the literature. Theunissen E...8头颈癌患者放化疗和放疗后的感觉神经性耳聋：文献系统评价
Sensorineural hearing loss in patients with head and neck cancer after chemoradiotherapy and radiotherapy： A systematic review of the literature. Theunissen EA,Bosma SC,Zuur CL,et al. Head Neck, 2013 Nov 7.
头颈癌患者放疗（RT）和以顺铂为主的放化疗（CRT）可以引起感觉神经性耳聋fSNHL）。该文旨在回顾CRT与RT引起的SNHL，并作进一步探究。以“放疗”，“耳毒性”，“头颈鳞癌”及其同义词为关键词，在Medline和Embase进行检索。展开更多
Objectives: Correlation between Narrow Band Chirp Auditory Brainstem Response (NB-CE chirp ABR) as a frequency specific method for hearing threshold detection in children and Tone burst Auditory Brainstem Response (Tb...Objectives: Correlation between Narrow Band Chirp Auditory Brainstem Response (NB-CE chirp ABR) as a frequency specific method for hearing threshold detection in children and Tone burst Auditory Brainstem Response (Tb-ABR) in reference to behavioral hearing threshold audiometry. Material and Methods: This study was conducted on 100 patients at audiology unit, Menoufia University hospital, in the period from Oct. 2015 to Feb. 2017. Patients enrolled within this study were classified into four groups: Group I: included 40 patients diagnosed with normal hearing, Group II: included 48 patients with mild to moderate sensorineural hearing loss (SNHL), Group III: included 56 patients with moderate to moderately severe SNHL and Group IV: included 56 patients with severe to profound SNHL. All patients’ enrolled NB-CE chirp ABR, Tb-ABR as well as pure tone audiogram (PTA). Results: there was a significant correlation between NB-CE chirp ABR and PTA hearing threshold. The use of a chirp-ABR testing ensures higher sensitivity and accuracy than that of Tb-ABR for measuring frequency-specific thresholds in young children. Conclusion: NB-CE Chirp ABR is more efficient than Tb-ABR as a frequency specific tool for hearing threshold estimation in children.展开更多
Background:Previous studies that assessed the association of sensorineural hearing loss(SNHL)and pseudoexfoliation syndrome(PEX)produced contradictory results.We conducted a meta-analysis to further evaluate this rela...Background:Previous studies that assessed the association of sensorineural hearing loss(SNHL)and pseudoexfoliation syndrome(PEX)produced contradictory results.We conducted a meta-analysis to further evaluate this relationship.Methods:Eligible studies that evaluated the association between SNHL and PEX were identified.Summary odds ratios(ORs)and 95%confidence intervals(CIs)were calculated employing random-effects models.Subgroup analysis and meta-regression were performed to assess heterogeneity by several covariates.Publication bias was tested by Begg’s funnel plot and Egger’s regression test.Results:A total of 14 eligible studies,involving 1,142 PEX patients and 9,914 controls,were included in this meta-analysis.Overall analysis showed that patients with PEX,when compared with the control group,experienced a significantly increased risk for hearing loss[OR:3.74(95%CI:2.56 to 5.47);P<0.001].Substantial heterogeneity was observed.Subgroup analysis revealed a decrease in this heterogeneity in ageand sex-matched studies and in studies that used the same definition of hearing loss.Meta-regression analysis showed that definition of hearing loss contributed to substantial heterogeneity(P=0.044).No evidence of publication bias was observed.Discussion:We found that PEX is associated with an increased risk of SNHL.The effect of PEX on the prevalence of hearing loss indicates that PEX is clearly a systemic disease with potential otological complications.展开更多
基金supported by the National Natural Science Foundation of China [grant numbers 31300624]Postdoctoral Science Foundation of China [grant numbers 2015M571818]+1 种基金Six Major Categories Talent [grant numbers 2014-WSN-043]Innovation and Entrepreneurship Training Program for College Student in Jiangsu Province [grant numbers 201510313003Z]
文摘Objective:To identify presence of inflammasome activated in mouse cochlea with sensorineural hearing loss (SNHL) caused by cytomegalovirus (CMV) infection. Method:MCMV was injected into the right cerebral hemisphere in neonatal BALB/c mice at 2000 pfu virus titers. Auditory brainstem responses (ABRs) were tested to evaluate hearing at 21 days. Histopathological studies were conducted to confirm localizations of MCMV infected cells in the inner ear. Expression of inflammasome related factors was assessed by immunofluorescence, Quantitative real-time PCR and Western blotting. Results:In the mouse model of CMV induced SNHL, inflammasome related kinase Caspase-1 and downstream inflammatory factor IL-1b and IL-18 were found increased and activated after CMV infection in the cochlea. These factors could further up-regulate expression of IL-6 and TNF-a. These inflammatory factors are neurotoxicity and may contribute to hearing impairment. Furthermore, we also detected significantly increased AIM2 protein that accumulated in the SGN of cochleae with CMV infection. Significance:We have shown that inflammasome as a novel inherent immunity mechanism may contribute to hearing impairment. Conclusion:Our data indicate that imflammasome assemble in mouse inner ear in response to CMV infection. We have revealed a novel pa-thology event in CMV induced SNHL involving activation of inflammasome in mouse cochlea. Additionally, we have shown that inflammasome may be a novel target for prevention and treatment of CMV related SNHL. Copyright ? 2016, The Authors. Production & hosting by Elsevier (Singapore) Pte Ltd On behalf of PLA General Hospital Department of OtolaryngologyHead and Neck Surgery. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
文摘Background: Drill and surgical tools can cause noise induced hearing loss when used on or adjacent to the ossicular chain and stapes footplate or while working on the mastoid bone. Therefore the drill generated noise has been incriminated as a cause of sensorineural hearing loss in the operated ear. Objective: This study has been undertaken to estimate the proportion of sensorineural hearing loss following middle ear surgery and to assess the bone conduction thresholds of operated ear following middle ear surgery at different follow up periods. Methods: 100 cases of chronic suppurative otitis media posted for middle ear exploration surgery were included in the study. Results: Sensorineural hearing loss was present in 3 cases out of 100 cases (3%) after undergoing mastoid surgery. There was a mean increase in bone conduction thresholds of 0.75 dB at 500 Hz, 1.4 dB at 1 kHz, 1.7 dB at 2 kHz and 2.7 dB at 4 kHz at 6th month of follow up period after surgery with statistical significance at 2 kHz (p = 0.011) and 4 kHz (p Conclusion: Post mastoidectomy hearing loss is of high frequency in nature.
文摘Aim: This study was carried out to assess association between Sensorineural hearing loss (SNHL) and Chronic Suppurative Otitis Media (CSOM), to identify possible risk factors and determine correlation of duration of CSOM and incidence of SNHL. Material and Method: The study consisted of 100 patients of either sex between 15 and 40 years of age, suffering from unilateral CSOM for the past three months or more, the normal ear served as control. Results: This study showed duration of symptoms is 18% patients for 5 - 10 years and below 5 years in 73% patients. It was found that mean BC thresholds were significantly higher in the diseased ears as compared to the control ears. Though the higher frequencies are more affected than the lower frequencies, in subtotal and attic perforations the speech frequencies seemed to be maximally affected. Conclusion: CSOM should be diagnosed early and should be managed effectively so as to prevent the chances of developing SNHL.
文摘8头颈癌患者放化疗和放疗后的感觉神经性耳聋：文献系统评价
Sensorineural hearing loss in patients with head and neck cancer after chemoradiotherapy and radiotherapy： A systematic review of the literature. Theunissen EA,Bosma SC,Zuur CL,et al. Head Neck, 2013 Nov 7.
头颈癌患者放疗（RT）和以顺铂为主的放化疗（CRT）可以引起感觉神经性耳聋fSNHL）。该文旨在回顾CRT与RT引起的SNHL，并作进一步探究。以“放疗”，“耳毒性”，“头颈鳞癌”及其同义词为关键词，在Medline和Embase进行检索。
文摘Objectives: Correlation between Narrow Band Chirp Auditory Brainstem Response (NB-CE chirp ABR) as a frequency specific method for hearing threshold detection in children and Tone burst Auditory Brainstem Response (Tb-ABR) in reference to behavioral hearing threshold audiometry. Material and Methods: This study was conducted on 100 patients at audiology unit, Menoufia University hospital, in the period from Oct. 2015 to Feb. 2017. Patients enrolled within this study were classified into four groups: Group I: included 40 patients diagnosed with normal hearing, Group II: included 48 patients with mild to moderate sensorineural hearing loss (SNHL), Group III: included 56 patients with moderate to moderately severe SNHL and Group IV: included 56 patients with severe to profound SNHL. All patients’ enrolled NB-CE chirp ABR, Tb-ABR as well as pure tone audiogram (PTA). Results: there was a significant correlation between NB-CE chirp ABR and PTA hearing threshold. The use of a chirp-ABR testing ensures higher sensitivity and accuracy than that of Tb-ABR for measuring frequency-specific thresholds in young children. Conclusion: NB-CE Chirp ABR is more efficient than Tb-ABR as a frequency specific tool for hearing threshold estimation in children.
基金the National Natural Science Foundation of China(No.82000890)Hainan Provincial Natural Science Foundation of China(No.820RC780)+1 种基金Young Talents’Science and Technology Innovation Project of Hainan Association for Science and Technology(No.QCXM202020)Hainan Province Clinical Medical Center.
文摘Background:Previous studies that assessed the association of sensorineural hearing loss(SNHL)and pseudoexfoliation syndrome(PEX)produced contradictory results.We conducted a meta-analysis to further evaluate this relationship.Methods:Eligible studies that evaluated the association between SNHL and PEX were identified.Summary odds ratios(ORs)and 95%confidence intervals(CIs)were calculated employing random-effects models.Subgroup analysis and meta-regression were performed to assess heterogeneity by several covariates.Publication bias was tested by Begg’s funnel plot and Egger’s regression test.Results:A total of 14 eligible studies,involving 1,142 PEX patients and 9,914 controls,were included in this meta-analysis.Overall analysis showed that patients with PEX,when compared with the control group,experienced a significantly increased risk for hearing loss[OR:3.74(95%CI:2.56 to 5.47);P<0.001].Substantial heterogeneity was observed.Subgroup analysis revealed a decrease in this heterogeneity in ageand sex-matched studies and in studies that used the same definition of hearing loss.Meta-regression analysis showed that definition of hearing loss contributed to substantial heterogeneity(P=0.044).No evidence of publication bias was observed.Discussion:We found that PEX is associated with an increased risk of SNHL.The effect of PEX on the prevalence of hearing loss indicates that PEX is clearly a systemic disease with potential otological complications.
