Peanut(Arachis hypogaea),which is widely cultivated across the world,provides high-quality vegetable oil,protein,dietary fiber,minerals,and vitamins for humans.However,in field conditions,the peanut is easily affected...Peanut(Arachis hypogaea),which is widely cultivated across the world,provides high-quality vegetable oil,protein,dietary fiber,minerals,and vitamins for humans.However,in field conditions,the peanut is easily affected by various biotic and abiotic stresses.Diplodia gossypina is the dominant pathogen causing severe collar rot on peanuts.To dissect the pathogenic mechanism of D.gossypina,genome sequencing analysis was performed by using the D.gossypina strain A20_4.The sequencing data showed that the genome assembly size of D.gossypina A20_4 is 43.03 Mb with a GC content of 54.91%.The de novo assembly identified a total of 10,745 genes,containing 41,526 coding sequences and 2.20%of repeat sequences,of which 6,461 genes(60.13%)were annotated using BlastP from GO annotation,3,245 genes(30.20%)and 3,093 genes(28.79%)were annotated from KOG and KEGG annotations,respectively.Meanwhile,the secreted proteins and effectors in 10,745 protein sequences encoded by the whole genome of D.gossypina A20_4 were analyzed,and the results showed that there are 790 secreted protein genes including 220 carbohydrate-active enzymes and 224 potential effector proteins.The functions of 222 potential effector proteins can be annotated by PHI-base.According to the annotation results,12 key pathogenic factors were identified in D.gossypina A20_4.Moreover,a serine/threonine protein kinase SNF1 gene required for autophagy process was identified and analyzed.Deciphering the whole genome of D.gossypina A20_4 provides us with novel insights into understanding evolution,pathogenic molecular mechanism,host-pathogen interaction,and many other complexities of the pathogen.展开更多
基金The Major Science and Technology Project of Henan Province(Grant No.201300111000)The Basic scientific Research Project of Henan Academy of Agricultural Sciences(Grant No.2022ZC37,2023ZC46)。
文摘Peanut(Arachis hypogaea),which is widely cultivated across the world,provides high-quality vegetable oil,protein,dietary fiber,minerals,and vitamins for humans.However,in field conditions,the peanut is easily affected by various biotic and abiotic stresses.Diplodia gossypina is the dominant pathogen causing severe collar rot on peanuts.To dissect the pathogenic mechanism of D.gossypina,genome sequencing analysis was performed by using the D.gossypina strain A20_4.The sequencing data showed that the genome assembly size of D.gossypina A20_4 is 43.03 Mb with a GC content of 54.91%.The de novo assembly identified a total of 10,745 genes,containing 41,526 coding sequences and 2.20%of repeat sequences,of which 6,461 genes(60.13%)were annotated using BlastP from GO annotation,3,245 genes(30.20%)and 3,093 genes(28.79%)were annotated from KOG and KEGG annotations,respectively.Meanwhile,the secreted proteins and effectors in 10,745 protein sequences encoded by the whole genome of D.gossypina A20_4 were analyzed,and the results showed that there are 790 secreted protein genes including 220 carbohydrate-active enzymes and 224 potential effector proteins.The functions of 222 potential effector proteins can be annotated by PHI-base.According to the annotation results,12 key pathogenic factors were identified in D.gossypina A20_4.Moreover,a serine/threonine protein kinase SNF1 gene required for autophagy process was identified and analyzed.Deciphering the whole genome of D.gossypina A20_4 provides us with novel insights into understanding evolution,pathogenic molecular mechanism,host-pathogen interaction,and many other complexities of the pathogen.
