该文通过RNA干扰方法下调染色体结构维持蛋白3(structural maintenance of chromosomes protein 3,SMC3)的表达,探究其对人肺癌细胞A549的影响。用Western blot验证SMC3敲低的效果,用CCK-8实验、Transwell实验检测SMC3敲低对A549细胞的...该文通过RNA干扰方法下调染色体结构维持蛋白3(structural maintenance of chromosomes protein 3,SMC3)的表达,探究其对人肺癌细胞A549的影响。用Western blot验证SMC3敲低的效果,用CCK-8实验、Transwell实验检测SMC3敲低对A549细胞的增殖和迁移能力的影响,用细胞成球等实验检测SMC3敲低对A549细胞干性的影响。结果显示,该研究成功地构建了p SUPERSMC3干扰质粒,将其转染A549细胞后SMC3蛋白质水平明显降低。实验组(p SUPER-SMC3)与正常对照组(control)及阴性对照组(p SUPER)相比,细胞增殖和迁移能力明显下降(P<0.01),细胞成球数减少,干性减弱。该实验结果表明,下调SMC3蛋白质水平可以抑制A549细胞的增殖和迁移能力,减弱细胞干性,提示SMC3对肺癌的发生和发展可能具有促进作用。该实验为寻找潜在的抗肺癌方法提供了新的实验依据。展开更多
So far quite a few ultraluminous X-ray(ULX) pulsars have been discovered.In this work,we construct a super-Eddington,magnetic accretion disk model to estimate the dipole magnetic field of eight ULX pulsars based on th...So far quite a few ultraluminous X-ray(ULX) pulsars have been discovered.In this work,we construct a super-Eddington,magnetic accretion disk model to estimate the dipole magnetic field of eight ULX pulsars based on their observed spin-up variations and luminosities.We obtain two branches of dipole magnetic field solutions.They are distributed in the range of B-(0.156-64.5) × 10^(10) G and-(0.275-79.0) × 10^(13) G corresponding to the low-and high-B solutions respectively.The low magnetic field solutions correspond to the state that the neutron stars are far away from the spin equilibrium,and the high magnetic field solutions are close to the spin equilibrium.The ultra-strong magnetic fields derived in Be-type ULX pulsars imply that the accretion mode in Be-type ULX pulsars could be more complicated than in the persistent ULX pulsars and may not be accounted for by the magnetized accretion disk model.We suggest that the transition between the accretor and the propeller regimes may be used to distinguish between the low-and high-B magnetic field solutions in addition to the detection of the cyclotron resonance scattering features.展开更多
文摘该文通过RNA干扰方法下调染色体结构维持蛋白3(structural maintenance of chromosomes protein 3,SMC3)的表达,探究其对人肺癌细胞A549的影响。用Western blot验证SMC3敲低的效果,用CCK-8实验、Transwell实验检测SMC3敲低对A549细胞的增殖和迁移能力的影响,用细胞成球等实验检测SMC3敲低对A549细胞干性的影响。结果显示,该研究成功地构建了p SUPERSMC3干扰质粒,将其转染A549细胞后SMC3蛋白质水平明显降低。实验组(p SUPER-SMC3)与正常对照组(control)及阴性对照组(p SUPER)相比,细胞增殖和迁移能力明显下降(P<0.01),细胞成球数减少,干性减弱。该实验结果表明,下调SMC3蛋白质水平可以抑制A549细胞的增殖和迁移能力,减弱细胞干性,提示SMC3对肺癌的发生和发展可能具有促进作用。该实验为寻找潜在的抗肺癌方法提供了新的实验依据。
基金supported by the National Key Research and Development Program of China (2016YFA0400803)the National Natural Science Foundation of China under grant Nos.11773015 and 10241301Project U1838201 supported by NSFC and CAS。
文摘So far quite a few ultraluminous X-ray(ULX) pulsars have been discovered.In this work,we construct a super-Eddington,magnetic accretion disk model to estimate the dipole magnetic field of eight ULX pulsars based on their observed spin-up variations and luminosities.We obtain two branches of dipole magnetic field solutions.They are distributed in the range of B-(0.156-64.5) × 10^(10) G and-(0.275-79.0) × 10^(13) G corresponding to the low-and high-B solutions respectively.The low magnetic field solutions correspond to the state that the neutron stars are far away from the spin equilibrium,and the high magnetic field solutions are close to the spin equilibrium.The ultra-strong magnetic fields derived in Be-type ULX pulsars imply that the accretion mode in Be-type ULX pulsars could be more complicated than in the persistent ULX pulsars and may not be accounted for by the magnetized accretion disk model.We suggest that the transition between the accretor and the propeller regimes may be used to distinguish between the low-and high-B magnetic field solutions in addition to the detection of the cyclotron resonance scattering features.
