Objective:Using neuromyelitis optica immunoglobulin G(NMO-IgG)to induced ex vivo mice spinal cord slice model.Methods:Vibratome-cut transverse spinal cord slices from 7-day-old C57BL/6Jmouse pups were cultured on tran...Objective:Using neuromyelitis optica immunoglobulin G(NMO-IgG)to induced ex vivo mice spinal cord slice model.Methods:Vibratome-cut transverse spinal cord slices from 7-day-old C57BL/6Jmouse pups were cultured on transwell porous supports for 7days,then randomly divided into the control group and NMO model group.Slices of the control group were further cultured with human serum complement,while slices from NMO model group were exposed to complement and NMO-IgG.After 24-hour incubation,slices of both groups were measured for aquaporin-4(AQP4),glial fibrillary acidic protein(GFAP),myelin basic protein(MBP)and neurofilament light chain(NFL)by immunofluorescence.Results:Slices exposed to NMO-IgG showed astrocyte swelling,and a significant loss of AQP4and GFAP staining.Ratios of the loss of AQP4and GFAP staining were 77.74%±6.75%and 75.62%±5.76%respectively in the model group,and NMO-like injury score was 3.11±0.60.But there were no obvious losses of AQP4and GFAP staining in the control group,and NMO-like lesion score was 0.00.There were significant differences between the two groups with regards to the above indexes(P<0.01).Ratios of the loss of MBP and NFL staining in the model group were 37.60%±4.88%and46.29%±4.98%respectively,while the corresponding figures in the control group were 9.10%±1.63%and 5.80%±0.81%,and the differences between the two groups were statistically significant(P<0.01).Conclusion:These results suggested that NMO-IgG-induced ex vivo spinal cord slice model possesses typical features of NMO,and this model might be useful for relevant fundamental studies.展开更多
This paper presents a new closure to slice models for evaluating slopes. The discussion is based on the minimal inter-slice action (MIA) hypothesis, which results in a new slice model without including artificially ad...This paper presents a new closure to slice models for evaluating slopes. The discussion is based on the minimal inter-slice action (MIA) hypothesis, which results in a new slice model without including artificially adjustable parameters. It has been realized that the new slice model predicts the minimum value of the safety factor, while all other slice models available always overestimate the value of the safety factor. Moreover, the gravity moment of each slice is found to be opposite to the overturning moment, which is different from the existing knowledge. In particular, the new slice model overcomes the situation where different assumptions of the inter-slice force function will give different safety factors to the same slope. The related numerical examples indicate that the new slice model can serve as a reliable tool for investigating geotechnical slope stability.展开更多
Abstract：reconstruction method using slice im-ages is proposed. Wanting to extract the outermost contours from slice images, the method of the improved GVF-Snake model with optimized force field and ray method is emp...Abstract：reconstruction method using slice im-ages is proposed. Wanting to extract the outermost contours from slice images, the method of the improved GVF-Snake model with optimized force field and ray method is employed. And then, the 3D model is reconstructed by contour connection using the im-proved shortest diagonal method and judgment function of contour fracture. The results show that the accuracy of reconstruction 3D model is improved.展开更多
In allusion to easy invalidation of damping valve in vehicle shock absorber caused by the impact from the road surface, the importance of the study of damping valve water hammer pressure is presented. The physical mod...In allusion to easy invalidation of damping valve in vehicle shock absorber caused by the impact from the road surface, the importance of the study of damping valve water hammer pressure is presented. The physical model of damping valve with the circle throttle slice is established. The time for the throttle slice deformation is studied by using the finite software, and the laws that how the structure parameters affect the deformation time are obtained. Combining the theory of water hammer, the water hammer initial and boundary condition of the damping valve is deduced, and the water hammer model of throttle slice is established. The analysis of simulation results indicates that the water hammer pressure amplitude and the amount of water hammer oscillation period can be reduced and the dependability of the valve can be enhanced by modifying the structure parameters and aperture width between slice and valve body.展开更多
A mathematical model for the inactivation of nitric oxide by rat cerebellar slices under non-steady state condition has been analyzed. This diffusion-inactivation model was used to estimate the kinetics of NO consumpt...A mathematical model for the inactivation of nitric oxide by rat cerebellar slices under non-steady state condition has been analyzed. This diffusion-inactivation model was used to estimate the kinetics of NO consumption by the rat cerebellar slices. He’s Homotopy perturbation method is used to solve the first order nonlinear differential equations which describe the concentrations given by net of diffusion and inactivation by the slices. Analytical expressions for the concentration of nitric oxide have been derived for all values of parameters. The obtained analytical results are compared with the simulation results (Matlab/Scilab program) and are found to be in good agreement.展开更多
A nonlinear finite element model is developed to examine the lateral behaviors of monopiles, which support offshore wind turbines(OWTs) chosen from five different offshore wind farms in Europe. The simulation is using...A nonlinear finite element model is developed to examine the lateral behaviors of monopiles, which support offshore wind turbines(OWTs) chosen from five different offshore wind farms in Europe. The simulation is using this model to accurately estimate the natural frequency of these slender structures, as a function of the interaction of the foundations with the subsoil. After a brief introduction to the wind power energy as a reliable alternative in comparison to fossil fuel, the paper focuses on concept of natural frequency as a primary indicator in designing the foundations of OWTs. Then the range of natural frequencies is provided for a safe design purpose. Next, an analytical expression of an OWT natural frequency is presented as a function of soil-monopile interaction through monopile head springs characterized by lateral stiffness KL, rotational stiffness KRand cross-coupling stiffness KLRof which the differences are discussed. The nonlinear pseudo three-dimensional finite element vertical slices model has been used to analyze the lateral behaviors of monopiles supporting the OWTs of different wind farm sites considered. Through the monopiles head movements(displacements and rotations), the values of KL, KRand KLRwere obtained and substituted in the analytical expression of natural frequency for comparison. The comparison results between computed and measured natural frequencies showed an excellent agreement for most cases. This confirms the convenience of the finite element model used for the accurate estimation of the monopile head stiffness.展开更多
Salmonella spp. surface transfer patterns/models between ready-to-eat (RTE) deli meat and a typical meat slicer are presented. A five-strain cocktail of Salmonella spp. was inoculated directly onto the rim of a round ...Salmonella spp. surface transfer patterns/models between ready-to-eat (RTE) deli meat and a typical meat slicer are presented. A five-strain cocktail of Salmonella spp. was inoculated directly onto the rim of a round slicer blade at an initial level of ca. 4, 5, 6, 7 or 8 log CFU/blade (ca. 3, 4, 5, 6 or 7 log CFU/cm2 of the blade edge area). Thereafter, a RTE deli meat (ham) was sliced to a thickness of 1.5 - 2.0 mm. In another cross-contamination scenario, a clean blade was initially used to slice ham, which was pre-surface-inoculated with the Salmonella cocktail (ca. 2, 3, 4, 5, 6 or 7 log CFU/cm2 with a total area of 100 cm2), followed by slicing of an un-inoculated ham. The log CFU of Salmonella per ham slice was determined and empirical models were developed. The models, follow the decreasing Power law, predict the surface cross-contamination of Salmonella spp. (at any initial level) for sliced deli meat (ham) and will provide a useful tool in developing RTE meat risk assessments. Surface transfer patterns of three foodborne pathogens, i.e. Listeria monocytogenes, E. coli O157:H7 and Salmonella predicted by models are also presented.展开更多
An algorithm for compensating the varying section contour within the extent of each layer thickness is proposed to improve the rapid prototyping part’s shape and size precision.
Method of obtaining landslide evaluating information by using Interferometric Synthetic Aperture Radar (InSAR) technique was discussed. More precision landslide surface deformation data extracted from InSAR image need...Method of obtaining landslide evaluating information by using Interferometric Synthetic Aperture Radar (InSAR) technique was discussed. More precision landslide surface deformation data extracted from InSAR image need take suitable SAR interferometric data selecting, path tracking, phase unwrapping processes. Then, the DEM model of scope and surface shape of the landslide was built. Combining with geological property of landslide and sliding displacements obtained from InSAR/D-InSAR images, a new landslide forecasting model called equal central angle slice method for those not obviously deformed landslides was put forward. This model breaks the limits of traditional research methods of geology. In this model, the landslide safety factor was calculated by equal central angle slice method, then considering the persistence ratio of the sliding surface based on plastic theory, the minimum safety factor was the phase when plastic area were complete persistence. This new model makes the application of InSAR/D-InSAR technology become more practical in geology hazard research.展开更多
提出一种切片Gibbs抽样的马尔可夫链蒙特卡罗(Markov chain Monte Carlo,MCMC)算法来计算空间误差模型未知参数的联合贝叶斯估计,通过2个模拟仿真说明提出的贝叶斯估计方法的有效性与切片Gibbs抽样算法的优势,实证分析说明模型和提出的...提出一种切片Gibbs抽样的马尔可夫链蒙特卡罗(Markov chain Monte Carlo,MCMC)算法来计算空间误差模型未知参数的联合贝叶斯估计,通过2个模拟仿真说明提出的贝叶斯估计方法的有效性与切片Gibbs抽样算法的优势,实证分析说明模型和提出的贝叶斯估计方法的有效性。展开更多
基金supported by the National Natural Science Foundation of China (No.81460194 No.81260188)
文摘Objective:Using neuromyelitis optica immunoglobulin G(NMO-IgG)to induced ex vivo mice spinal cord slice model.Methods:Vibratome-cut transverse spinal cord slices from 7-day-old C57BL/6Jmouse pups were cultured on transwell porous supports for 7days,then randomly divided into the control group and NMO model group.Slices of the control group were further cultured with human serum complement,while slices from NMO model group were exposed to complement and NMO-IgG.After 24-hour incubation,slices of both groups were measured for aquaporin-4(AQP4),glial fibrillary acidic protein(GFAP),myelin basic protein(MBP)and neurofilament light chain(NFL)by immunofluorescence.Results:Slices exposed to NMO-IgG showed astrocyte swelling,and a significant loss of AQP4and GFAP staining.Ratios of the loss of AQP4and GFAP staining were 77.74%±6.75%and 75.62%±5.76%respectively in the model group,and NMO-like injury score was 3.11±0.60.But there were no obvious losses of AQP4and GFAP staining in the control group,and NMO-like lesion score was 0.00.There were significant differences between the two groups with regards to the above indexes(P<0.01).Ratios of the loss of MBP and NFL staining in the model group were 37.60%±4.88%and46.29%±4.98%respectively,while the corresponding figures in the control group were 9.10%±1.63%and 5.80%±0.81%,and the differences between the two groups were statistically significant(P<0.01).Conclusion:These results suggested that NMO-IgG-induced ex vivo spinal cord slice model possesses typical features of NMO,and this model might be useful for relevant fundamental studies.
文摘This paper presents a new closure to slice models for evaluating slopes. The discussion is based on the minimal inter-slice action (MIA) hypothesis, which results in a new slice model without including artificially adjustable parameters. It has been realized that the new slice model predicts the minimum value of the safety factor, while all other slice models available always overestimate the value of the safety factor. Moreover, the gravity moment of each slice is found to be opposite to the overturning moment, which is different from the existing knowledge. In particular, the new slice model overcomes the situation where different assumptions of the inter-slice force function will give different safety factors to the same slope. The related numerical examples indicate that the new slice model can serve as a reliable tool for investigating geotechnical slope stability.
基金Supported by National Natural Science Foundation of China(No.61272286)
文摘Abstract：reconstruction method using slice im-ages is proposed. Wanting to extract the outermost contours from slice images, the method of the improved GVF-Snake model with optimized force field and ray method is employed. And then, the 3D model is reconstructed by contour connection using the im-proved shortest diagonal method and judgment function of contour fracture. The results show that the accuracy of reconstruction 3D model is improved.
基金Sponsored by the Ministerial Level Advanced Research Foundation (623010202 4)
文摘In allusion to easy invalidation of damping valve in vehicle shock absorber caused by the impact from the road surface, the importance of the study of damping valve water hammer pressure is presented. The physical model of damping valve with the circle throttle slice is established. The time for the throttle slice deformation is studied by using the finite software, and the laws that how the structure parameters affect the deformation time are obtained. Combining the theory of water hammer, the water hammer initial and boundary condition of the damping valve is deduced, and the water hammer model of throttle slice is established. The analysis of simulation results indicates that the water hammer pressure amplitude and the amount of water hammer oscillation period can be reduced and the dependability of the valve can be enhanced by modifying the structure parameters and aperture width between slice and valve body.
文摘A mathematical model for the inactivation of nitric oxide by rat cerebellar slices under non-steady state condition has been analyzed. This diffusion-inactivation model was used to estimate the kinetics of NO consumption by the rat cerebellar slices. He’s Homotopy perturbation method is used to solve the first order nonlinear differential equations which describe the concentrations given by net of diffusion and inactivation by the slices. Analytical expressions for the concentration of nitric oxide have been derived for all values of parameters. The obtained analytical results are compared with the simulation results (Matlab/Scilab program) and are found to be in good agreement.
文摘A nonlinear finite element model is developed to examine the lateral behaviors of monopiles, which support offshore wind turbines(OWTs) chosen from five different offshore wind farms in Europe. The simulation is using this model to accurately estimate the natural frequency of these slender structures, as a function of the interaction of the foundations with the subsoil. After a brief introduction to the wind power energy as a reliable alternative in comparison to fossil fuel, the paper focuses on concept of natural frequency as a primary indicator in designing the foundations of OWTs. Then the range of natural frequencies is provided for a safe design purpose. Next, an analytical expression of an OWT natural frequency is presented as a function of soil-monopile interaction through monopile head springs characterized by lateral stiffness KL, rotational stiffness KRand cross-coupling stiffness KLRof which the differences are discussed. The nonlinear pseudo three-dimensional finite element vertical slices model has been used to analyze the lateral behaviors of monopiles supporting the OWTs of different wind farm sites considered. Through the monopiles head movements(displacements and rotations), the values of KL, KRand KLRwere obtained and substituted in the analytical expression of natural frequency for comparison. The comparison results between computed and measured natural frequencies showed an excellent agreement for most cases. This confirms the convenience of the finite element model used for the accurate estimation of the monopile head stiffness.
文摘Salmonella spp. surface transfer patterns/models between ready-to-eat (RTE) deli meat and a typical meat slicer are presented. A five-strain cocktail of Salmonella spp. was inoculated directly onto the rim of a round slicer blade at an initial level of ca. 4, 5, 6, 7 or 8 log CFU/blade (ca. 3, 4, 5, 6 or 7 log CFU/cm2 of the blade edge area). Thereafter, a RTE deli meat (ham) was sliced to a thickness of 1.5 - 2.0 mm. In another cross-contamination scenario, a clean blade was initially used to slice ham, which was pre-surface-inoculated with the Salmonella cocktail (ca. 2, 3, 4, 5, 6 or 7 log CFU/cm2 with a total area of 100 cm2), followed by slicing of an un-inoculated ham. The log CFU of Salmonella per ham slice was determined and empirical models were developed. The models, follow the decreasing Power law, predict the surface cross-contamination of Salmonella spp. (at any initial level) for sliced deli meat (ham) and will provide a useful tool in developing RTE meat risk assessments. Surface transfer patterns of three foodborne pathogens, i.e. Listeria monocytogenes, E. coli O157:H7 and Salmonella predicted by models are also presented.
基金Supported by College Science Foundation of Jiangsu(07KJB460113)
文摘An algorithm for compensating the varying section contour within the extent of each layer thickness is proposed to improve the rapid prototyping part’s shape and size precision.
基金Project(BK2006171) supported by the Natural Sciences Foundation of Jiangsu Province
文摘Method of obtaining landslide evaluating information by using Interferometric Synthetic Aperture Radar (InSAR) technique was discussed. More precision landslide surface deformation data extracted from InSAR image need take suitable SAR interferometric data selecting, path tracking, phase unwrapping processes. Then, the DEM model of scope and surface shape of the landslide was built. Combining with geological property of landslide and sliding displacements obtained from InSAR/D-InSAR images, a new landslide forecasting model called equal central angle slice method for those not obviously deformed landslides was put forward. This model breaks the limits of traditional research methods of geology. In this model, the landslide safety factor was calculated by equal central angle slice method, then considering the persistence ratio of the sliding surface based on plastic theory, the minimum safety factor was the phase when plastic area were complete persistence. This new model makes the application of InSAR/D-InSAR technology become more practical in geology hazard research.
