Sri Lankan cassava mosaic virus(SLCMV)is a prominent causative agent of cassava mosaic disease in Asia and relies on the whitefly Bemisia tabaci cryptic complex for its transmission.However,the molecular mechanisms in...Sri Lankan cassava mosaic virus(SLCMV)is a prominent causative agent of cassava mosaic disease in Asia and relies on the whitefly Bemisia tabaci cryptic complex for its transmission.However,the molecular mechanisms involved in SLCMV transmission by B.tabaci have yet to be understood.In this study,we identified an aminopeptidase N-like protein(BtAPN)in B.tabaci Asia II 1,an efficient vector of SLCMV,which is involved in the SLCMV transmission process.Through the use of glutathione S-transferase pull-down assay and LC-MS/MS analysis,we demonstrated the interaction between BtAPN and the coat protein(CP)of SLCMV.This interaction was further confirmed in vitro,and we observed an induction of BtAPN gene expression following SLCMV infection.By interfering with the function of BtAPN,the quantities of SLCMV were significantly reduced in various parts of B.tabaci Asia II 1,including the whole body,midgut,hemolymph,and primary salivary gland.Furthermore,we discovered that BtAPN is conserved in B.tabaci Middle East-Asia Minor 1(MEAM1)and interacts with the CP of tomato yellow leaf curl virus(TYLCV),a begomovirus known to cause severe damage to tomato production.Blocking BtAPN with antibody led to a significant reduction in the quantities of TYLCV in whitefly whole body and organs/tissues.These results demonstrate that BtAPN plays a generic role in interacting with the CP of begomoviruses and positively regulates their acquisition by the whitefly.展开更多
Cassava(Manihot esculenta Crantz)is a major staple food crop for more than a billion people in the world.Cassava mosaic virus(CMV),belonging to the Geminiviridae family,is a primary threat to cassava production.Sri La...Cassava(Manihot esculenta Crantz)is a major staple food crop for more than a billion people in the world.Cassava mosaic virus(CMV),belonging to the Geminiviridae family,is a primary threat to cassava production.Sri Lankan cassava mosaic virus(SLCMV)is the only emergent CMV prevalent in South Asia and Southeast Asia since its identification in 2002.We reported the identification of two invasive strains of SLCMV,Col and HN7,in China in 2018.However,the occurrence and distribution of these known SLCMV strains and the presence of unknown geminivirus in China are still elusive.In this study,we firstly reported an improved CMV detection system based on molecular and serological methods,which was further used to determine the distribution of CMV in major cassava plantations in China.Two optimized PCR primer pairs based on the conserved regions of AV1 and AC1 genes were designed to detect different CMV species and distinguish SLCMV simultaneously.For a serological method,a polyclonal antibody against SLCMV AV1-encoded capsid protein was raised and used for enzyme-linked immunosorbent assay(ELISA).Consistent detection results were achieved by PCR-and ELISA-based methods.Among 62 examined samples collected in 2018,10 were SLCMV positive,with 4 coinfection cases of two strains(HN7 and Col)in the same cassava plant.Two primer pairs could also be used to detect the presence of CMV in whitefly(Bemisia tabaci)sensitively.All positive samples were from Fujian and Hainan Provinces,indicating a limited distribution of SLCMV in cassava plants in China.Our detection methods could be used for future surveillance system to control and manage cassava mosaic disease in China and other countries.展开更多
基金Financial support for this article was provided by the National Natural Science Foundation of China(32161143008,31925233)the Bill&Melinda Gates Foundation(Investment ID OPP1149777)Wilmer J.Cuellar,is supported by the Australian Centre for International Agricultural Research(ACIAR),and the One CGIAR initiative on Plant Health(PHI).
文摘Sri Lankan cassava mosaic virus(SLCMV)is a prominent causative agent of cassava mosaic disease in Asia and relies on the whitefly Bemisia tabaci cryptic complex for its transmission.However,the molecular mechanisms involved in SLCMV transmission by B.tabaci have yet to be understood.In this study,we identified an aminopeptidase N-like protein(BtAPN)in B.tabaci Asia II 1,an efficient vector of SLCMV,which is involved in the SLCMV transmission process.Through the use of glutathione S-transferase pull-down assay and LC-MS/MS analysis,we demonstrated the interaction between BtAPN and the coat protein(CP)of SLCMV.This interaction was further confirmed in vitro,and we observed an induction of BtAPN gene expression following SLCMV infection.By interfering with the function of BtAPN,the quantities of SLCMV were significantly reduced in various parts of B.tabaci Asia II 1,including the whole body,midgut,hemolymph,and primary salivary gland.Furthermore,we discovered that BtAPN is conserved in B.tabaci Middle East-Asia Minor 1(MEAM1)and interacts with the CP of tomato yellow leaf curl virus(TYLCV),a begomovirus known to cause severe damage to tomato production.Blocking BtAPN with antibody led to a significant reduction in the quantities of TYLCV in whitefly whole body and organs/tissues.These results demonstrate that BtAPN plays a generic role in interacting with the CP of begomoviruses and positively regulates their acquisition by the whitefly.
基金supported by the National Science Foundation of China(31830073,31672001 and 31901853)National key research and development program(2018YFD0201503).
文摘Cassava(Manihot esculenta Crantz)is a major staple food crop for more than a billion people in the world.Cassava mosaic virus(CMV),belonging to the Geminiviridae family,is a primary threat to cassava production.Sri Lankan cassava mosaic virus(SLCMV)is the only emergent CMV prevalent in South Asia and Southeast Asia since its identification in 2002.We reported the identification of two invasive strains of SLCMV,Col and HN7,in China in 2018.However,the occurrence and distribution of these known SLCMV strains and the presence of unknown geminivirus in China are still elusive.In this study,we firstly reported an improved CMV detection system based on molecular and serological methods,which was further used to determine the distribution of CMV in major cassava plantations in China.Two optimized PCR primer pairs based on the conserved regions of AV1 and AC1 genes were designed to detect different CMV species and distinguish SLCMV simultaneously.For a serological method,a polyclonal antibody against SLCMV AV1-encoded capsid protein was raised and used for enzyme-linked immunosorbent assay(ELISA).Consistent detection results were achieved by PCR-and ELISA-based methods.Among 62 examined samples collected in 2018,10 were SLCMV positive,with 4 coinfection cases of two strains(HN7 and Col)in the same cassava plant.Two primer pairs could also be used to detect the presence of CMV in whitefly(Bemisia tabaci)sensitively.All positive samples were from Fujian and Hainan Provinces,indicating a limited distribution of SLCMV in cassava plants in China.Our detection methods could be used for future surveillance system to control and manage cassava mosaic disease in China and other countries.
